New Polypeptides from Chinese Mistletoe, Viscum coloratum （Kom.） Nakai

Mistletoes are perennial evergreen, semi-parasitic plants growing on branches or stems of deciduous trees. As a medical plant, mistletoe has the reputation of “magic herb” in Europe and has been used for numerous conditions. Its aqueous extractions have…     

Sebiferone,a New Triterpenoid from Stem Bark of Sapium sebiferum （L.）Roxb.

From the stem bark of Sapium sebiferum a new triterpenoid,anmed sebiferone (1),was isolated.The structure of the new compound was clucidated as 3β-acetoxy-D-friedoolen-14-en-1-one-28-oic acid on the basis of spectral and chemical methods.     

槲寄生药材的高效液相色谱指纹图谱研究

采用Diamonsil C<,18>(2)色谱柱,乙腈-0.1%磷酸梯度洗脱,测定了15批不同产地的槲寄生样品,建立其指纹图谱共有模式;采用"中药色谱指纹图谱相似度评价系统"软件进行数据处理,聚类分析,建立槲寄生药材高效液相色谱指纹图谱.结果表明:以高圣草素-7-O-β-D-芹菜糖基(1→2)-β-D-葡萄糖苷为参照物...     

Purification and Primary Structure Determination of a Novel Polypeptide Isolated from Mistletoe Viscum coloratum

A novel polypeptide was isolated from mistletoe Viscum coloratum. The primarystructure of the polypeptide ‘named viscotoxin B2‘ was determined to be KSCCKNTTGRNIYNT CRFAGGSRERCAKLSGCKIISASTCPSDYPK by Edman degradation. Viscotoxin B2 shared highsequence homology with viscotoxins isolated from Viscum album. Pharmacological experimentsshowed that viscotoxin B2 had distinct cytotoxic activity on tumor cells. Viscotoxin B2 could beused as a leading compound in cancer therapy.     

A novel plant growth regulator from Pholiota lubrica

Nine compounds including a new cinnamamide (1), N-(1-cinnamoylpyrrolidin-2-yl)cinnamamide, and two compounds (8 and 9) first isolated from natural sources, were obtained from the edible mushroom Pholiota lubrica. Their structures were determined by the interpretation of spectroscopic data. Compounds 1, 3 and 9 exhibited the inhibitory activity against lettuce, while compounds 2 and 7 promoted the growth of lettuce.     

Asymmetric bioreduction of substituted acenaphthenequinones using plant enzymatic systems： A novel strategy for the preparation of （＋）- and （-）-mono hydroxyacenaphthenones

Regio- and enantioselective reduction of substituted acenaphthenequinones were conducted under mild reaction conditions using plant enzymatic systems. A screening of 15 plants allowed the selection of two suitable plants fulfilling enantiocomple- mentarity. The （＋）- and （-）-mono hydroxyacenaphthenones were achieved with high conversion and good enantiomeric purity using peach （Prunus persica （L.） Batsch., conversion 98%, 71% ee） and carrot （Daucus carota L., conversion 95%, 81% ee）, respectively.     

A novel flavonoid from the root of Macrothelypteris torresiana （Gaud.） Ching

A novel flavonoid was isolated from the root of Macrothelypteris torresiana(Gaud.) Ching.The structure of the product was identified as 5,7-dihydroxy-2-(1,2-isopropyldioxy-4-oxocyclohex-5-enyl)-chromen-4-one on the ground of chemical and spectroscopic methods.     

Vaccariside A, Novel Saponin from Vaccaria segetalis (Neck.) Garcke

TheseedsofVaccariasegetalis(Neck.)Garcke,belongingtocaryophyllaceaefamily,isawell-knowncommonly-usedtraditionalChinesemedicine(TCM)called'wang-bu-fluxing',andhasbeenrecordedinChinesePharmacopoeiaasafolkmedicineforthetreatmentofblood-stasissyndromewithameniaordysmenorrhea,carbuncle,stranguriacomplicatedbyhematuria.Severaltriterpenoidsaponinsandeightcyclopeptideshavebeenisolatedfromtheseedstogetherwithmanyothercompoundsl-4.Duringourfurthersearchingforbioactivesecondarymetabolitesinthisplant,a…     

A novel antimicrobial flavonoid from the stem bark of Commiphora pedunculata (Kotschy & Peyr.) Engl.

A new flavonoid, 2-(3,5-dihydroxy-4-methoxy-phenyl)-3,5-dihydroxy-8,8-dimethyl-2,3-dihydro-8H-pyrano[3,2]chromen-4-one, together with previously reported epicatechin was isolated from the ethyl acetate soluble fraction of the methanol extract of the stem bark of Commiphora pedunculata. The structures of these compounds were elucidated based on extensive analysis of their spectral data, including 1 and 2D NMR. The compounds were active against 9 out of 12 tested microorganisms including a resistant strain; vancomycin-resistant entrococci (VRE), Escherichia coli, Staphylococcus aureus and Candida albicans. The zones of inhibition ranged between 22 and 34 mm against the microorganisms. The minimum inhibitory concentration was as low as 6.25 μg/mL against Shigella dysentriae, Bacillus cereus and S. aureus while the minimum bactericidal concentration was as low as 50 μg/mL against Pseudomonas aeruginosa, VRE and C. albicans. This is the first report of the isolation of the compound.     

Anti-inflammatory,antioxidant and antiangiogenic activities of diosgenin isolated from traditional medicinal plant,Costus speciosus (Koen ex.Retz.) Sm

Costus speciosus is an important medicinal plant widely used in several indigenous medicinal formulations. The present study was conducted to evaluate the in vitro anti-inflammatory, antioxidant and antiangiogenic activities of diosgenin isolated from C. speciosus. The diosgenin was isolated from C. speciosus by HPTLC and its biological activities were studied by different protocols. The results demonstrated that LPS stimulated TNF-α generation in RAW 264.7 macrophage culture supernatant up to 3.7-fold of the control and that sample treatment (50 μg/mL) resulted in a highly significant inhibitory effect on LPS-stimulated TNF-α (p < 0.01) in a similar manner to methotrexate inhibitory effect. The tested sample possessed an effective antioxidant scavenging affinity against DPPH radicals as compared with the standard antioxidant activity of vitamin C. The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer, apoptotic and inhibitory effects on cell proliferation.