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Preeclapsia (PE) is a severe disorder that occurs during pregnancy, leading to maternal and fetal morbidity and mortality. PE affects about 3-8% of all pregnancies. In this study, we conducted liquid chromatography- mass spectrometry/mass spectrometry (LC-MS/MS) to analyze serum samples depleted of the six most abundant proteins from normal and PE-affected pregnancies to profile serum proteins. A total of 237 proteins were confidently identified with <1% false discovery rate from the two groups of duplicate analysis. The expression levels of those identified proteins were compared semiquantitatively by spectral counting. To further validate the candidate proteins with a quantitative mass spectrometric method, selective reaction monitoring (SRM) and enzyme linked immune assay (ELISA) of serum samples collected from pregnant women with severe PE (n = 8) or normal pregnant women (n = 5) was conducted. α2- HS-glycoprotein (AHSG), retinol binding protein 4 (RBP4) and α-1-microglobulin/bikunin (AMBP) and Insulin like growth factor binding protein, acid labile subunit (IGFBP-ALS) were confirmed to be differentially expressed in PE using SRM (P<0.05). Among these proteins, AHSG was verified by ELISA and showed a statistically significant increase in PE samples when compared to controls. 相似文献
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Nazar Manko Marina Starykovych Yaroslav Bobak Rostyslav Stoika Vladimir Richter Olga Koval Inna Lavrik Daniel Hork Serhiy Souchelnytskyi Yuriy Kit 《Biomedical chromatography : BMC》2019,33(11)
The cytopoxic effect of RL2 lactaptin (the recombinant analog of proteolytic fragment of human kappa‐casein) toward tumor cells in vitro and in vivo presents it as a novel promising antitumor drug. The binding of any drug with serum proteins can affect their activity, distribution, rate of excretion and toxicity in the human body. Here, we studied the ability of RL2 to bind to various blood serum proteins. Using magnetic microparticles bearing by RL2 as an affinity matrix, in combination with mass spectrometry and western blot analysis, we found a number of blood serum proteins possessing affinity for RL2. Among them IgA, IgM and IgG subclasses of immunoglobulins, apolipoprotein A1 and various cortactin isoforms were identified. This data suggests that in the bloodstream RL2 lactaptin takes part in complicate protein–protein interactions, which can affect its activity. 相似文献
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Dr. Tristan Doussineau Dr. Carole Mathevon Dr. Lucie Altamura Dr. Charlotte Vendrely Dr. Philippe Dugourd Dr. Vincent Forge Dr. Rodolphe Antoine 《Angewandte Chemie (International ed. in English)》2016,55(7):2340-2344
Amyloid fibrils are self‐assembled protein structures with important roles in biology (either pathogenic or physiological), and are attracting increasing interest in nanotechnology. However, because of their high aspect ratio and the presence of some polymorphism, that is, the possibility to adopt various structures, their characterization is challenging and basic information such as their mass is unknown. Here we show that charge‐detection mass spectrometry, recently developed for large self‐assembled systems such as viruses, provides such information in a straightforward manner. 相似文献
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Alfred Böttcher Christoph Möllers Karl J. Lackner Prof. Dr. med. Gerd Schmitz 《Electrophoresis》1998,19(7):1110-1116
An automated free-solution isotachophoresis system (FS-ITP) for preparative fractionation of biopolymers is described, operated in a batch mode. The dimension of the separation chamber allows an up to 1200-fold higher sample load compared to separation in capillaries of 180 μm inner diameter as used in analytical capillary isotachophoresis (C-ITP). The preparative capacity of the system is within the milligram range. The method is fully compatible with analytical C-ITP, which is essential for preparative-scale isotachophoresis with regard to optimization of electrolyte systems and the search for suitable spacers. As a model application the fractionation of human serum proteins is reported. The collected fractions were analyzed by C-ITP and agarose gel electrophoresis. 相似文献
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质谱在肽和蛋白质序列分析中的应用 总被引:7,自引:0,他引:7
了解肽和蛋白质的序列对理解其功能具有重要意义,测定其序列也是当前生命 科学研究中的重要内容之一,质谱作为高灵敏度的测定分子结构的仪器,其高灵敏 度、广泛的适用性及快速性等特性使它具有很大潜力发展成为辅助传统测序方法的 新方法,并得到了广泛的关注。从离子活化方法(包括碰撞诱导解离CID、源后裂 解PSD、源内裂解ISD等)、衍生化作用以及氨基酸残基消除方式(高能活化产生亚 稳离子、化学降解、酶降解)等多个角度介绍了利用质谱分析多肽和蛋白质序列的 方法,并对其发展前景作出展望。 相似文献
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Pesavento IC Bertazzo A Flamini R Vedova AD De Rosso M Seraglia R Traldi P 《Journal of mass spectrometry : JMS》2008,43(2):234-241
Until now the study of pathogenic related proteins in grape juice and wine, performed by ESI-MS, LC/ESI-MS, and MALDI/MS, has been proposed for differentiation of varieties. In fact, chitinases and thaumatin-like proteins persist through the vinification process and cause hazes and sediments in bottled wines. An additional instrument, potentially suitable for the grape varieties differentiation, has been developed by MALDI/MS for the grape seed protein analysis. The hydrosoluble protein profiles of seeds extract from three different Vitis vinifera grape (red and white) varieties were analyzed and compared. In order to evaluate the environmental conditions and harvest effects, the seed protein profiles of one grape variety from different locations and harvests were studied. 相似文献
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Fabio Gomes Betsegaw Lemma Dulith Abeykoon Dapeng Chen Yan Wang David Fushman Catherine Fenselau 《Journal of mass spectrometry : JMS》2019,54(1):19-25
A strategy for top‐down analysis of branched proteins has been reported earlier, which relies on electron transfer dissociation assisted by collisional activation, and software designed for graphic interpretation of tandem mass spectra and adapted for branched proteins. In the present study, the strategy is applied to identify unknown and novel products of reactions in which rationally mutated proteoforms of Rub1 are used to probe the selectivity of E1 and E2 enzymes normally active in ubiquitination. To test and demonstrate this application, components and attachment sites of three branched dimers are deduced and the mutations are confirmed. 相似文献
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同位素稀释热电离质谱法测定人血清中痕量铜和锌 总被引:7,自引:0,他引:7
采用热电离同位素稀释质谱法(ID-TIMS)准确测定了欧盟标准物质与测量研究院(EC-JRC-IRMM)组织的国际测量评估计划IMEP-17人血清样品中的痕量铜和锌。由于锌和铜都是易受污染的元素,本工作建立了仅用少量硝酸消解的低流程本底和适于热电离质谱测量的生物基体血清中痕量铜和锌的样品前处理方法;采用适当比例的硅胶和磷酸作为电离增强剂,在热电离质谱(TIMS)测量时获得了较高强度且稳定的铜和锌离子束;血清中痕量铜和锌的测量结果可直接溯源到国际单位mole。2种人血清样品中铜和锌测量结果的不确定度(k=2)分别为0.94%、0.83%和0.49%,测量值被EC-JRC-IRMM采用作为该样品的标准值。 相似文献
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Development of simultaneous analysis of tryptophan metabolites in serum and gastric juice – an investigation towards establishing a biomarker test for gastric cancer diagnosis 下载免费PDF全文
Jong Min Choi Won Sang Park Kyo Young Song Hwa Jeong Lee Byung Hwa Jung 《Biomedical chromatography : BMC》2016,30(12):1963-1974
To evaluate changes in tryptophan metabolism and discover diagnostic biomarkers for gastric cancer, a quantitative method was developed for tryptophan and its seven metabolites (indole‐3‐lactic acid, anthranilic acid, serotonin, nicotinic acid, kynurenic acid, kynurenine and 3‐indoxyl sulfate) in both human serum and gastric juice using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Serum and gastric juice were prepared with a simple protein precipitation using aqueous 0.1% formic acid and acetonitrile. As a result, it was found that the kynurenine pathway of tryptophan metabolism was activated in gastric cancer and that the metabolic ratio of kynurenine/tryptophan, which reflects the enzyme activity of indoleamine‐2,3‐dioxygenase, was associated with the observed metabolic changes. Finally, the investigation of tryptophan metabolites, especially kynurenic acid, in serum and gastric juice might serve as biomarkers for gastric cancer. The findings in this study provide critical information of tryptophan metabolism which can be applied to a serum‐based diagnostic test for gastric cancer. 相似文献
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《Journal of mass spectrometry : JMS》2018,53(7):ii-ii
Ambient surface mass spectrometry is an emerging field which shows great promise for the analysis of biomolecules directly from their biological substrate. In this article, we describe ambient ionisation mass spectrometry techniques for the in situ analysis of intact proteins. As a broad approach, the analysis of intact proteins offers unique advantages for the determination of primary sequence variations and posttranslational modifications, as well as interrogation of tertiary and quaternary structure and protein‐protein/ligand interactions. In situ analysis of intact proteins offers the potential to couple these advantages with information relating to their biological environment, for example, their spatial distributions within healthy and diseased tissues. Here, we describe the techniques most commonly applied to in situ protein analysis (liquid extraction surface analysis, continuous flow liquid microjunction surface sampling, nano desorption electrospray ionisation, and desorption electrospray ionisation), their advantages, and limitations and describe their applications to date. We also discuss the incorporation of ion mobility spectrometry techniques (high field asymmetric waveform ion mobility spectrometry and travelling wave ion mobility spectrometry) into ambient workflows. Finally, future directions for the field are discussed. 相似文献
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报道了血样中异烟肼的串联质谱快速分析,利用这种分析方法,证实了一男性死者血样中异烟肼及其代谢产物的存在,从而为法庭诉讼提供了可靠的证据。这种方法具有快速性,高灵敏度和高选择性,适合于需要快速分析的场合。 相似文献
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Marta Zalewska Agnieszka Gawin Milena Ściskalska Marcin Moczulski Halina Milnerowicz 《International journal of environmental analytical chemistry》2013,93(14-15):1422-1434
Metallurgy processes are associated with many hazardous and toxic factors, including heavy metals. Exposure to heavy metals can cause damage to different organs, which can be observed through variation in the concentration of proteins in serum. The aim of this study was to evaluate the changes in a serum protein profile of copper smelters exposed to As, Cd and Pb ions, and xenobiotics present in tobacco smoke. A 2.3-fold higher Pb concentration in the blood and a 2.8-fold greater As concentration have been observed in the urine of non-smoking smelters compared to a control group. In the blood of smoking smelters, Cd concentration was 2-fold higher than in non-smoking ones. Serum proteins were separated by capillary electrophoresis, and in the group of non-smoking smelters, a higher amount of α1-globulins was observed. In the group of smoking smelters, fewer α1-globulins were noted. Furthermore, a greater amount of α2-globulins in the serum of smoking and non-smoking workers in relation to the control group was revealed. A positive correlation between the concentration of Cd in the blood and the content of a fraction containing α1- and α2-globulins was revealed. Urine Cd concentration was found to be negatively associated with the α1- and α2-globulins fraction. Observed abnormalities in the proteins profiles of smelters can be important markers when assessing exposure to heavy metals and in the early diagnosis of diseases caused by them. 相似文献
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Mass spectrometry in the structural analysis of flavonoids 总被引:5,自引:0,他引:5
Flavonoids are very common and widespread secondary plant metabolites. They have a wide range of biological and physiological activities and serve as chemotaxonomic marker compounds. Therefore, they have been extensively investigated both in the past and during recent years. The interest in them is still increasing. In the search for new compounds, and also in quality control, there is a need to have reliable methodology for the analysis of flavonoids. Mass spectrometry can make an invaluable contribution because of its high sensitivity, possibilities of coupling with liquid chromatography and the availability of powerful tandem mass spectrometric techniques. A review of currently available mass spectrometric methodology used in the structure elucidation of flavonoids is presented. Sample preparation, liquid chromatographic/mass spectrometric analysis and tandem mass spectrometric procedures for the characterization of flavonoid aglycones, O-glycosides, C-glycosides and acylated glycosides are considered. 相似文献
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In this paper, we review the mass probes used for the derivation of a variety of biomolecules efficiently detected by the electrospray ionization-mass spectrometry and mass probe-assisted ionization method for total analysis and determination by consecutive detection with a single instrument. We describe mass probes for a variety of molecules including proteins, nucleobases, metallic cations, and other small molecules. 相似文献