钼磷酸杂多蓝分光光度法测定异烟肼含量

研究异烟肼与钼磷酸杂多蓝反应的条件.在水介质中,异烟肼与钼磷酸在90C时发生荷移反应,标题化合物的最大吸收波长为700nm.IR数据显示化合物依然保持Keggin结构.表观摩尔吸光系数为5.26×108L·mol-1·cm-1,异烟肼的浓度在4.00-24.0mg·L-1范围内与吸光度呈线性关系,相对标准偏差为0.44%(n=6).回收率在97%以上.用本法测定了异烟肼片剂中有效成分的含量,并与文献值进行比较,二者结果吻合.     

流动注射抑制化学发光法测定异烟肼

以异烟肼与K3 Fe(CN) 6的氧化还原反应对Luminol K3 Fe(CN) 6的化学发光反应的抑制为基础 ,设计出一种简便、快速、灵敏度高的流动注射抑制化学发光测定异烟肼的新方法。测定线性范围为 1 4× 10 -3 ～5 5 μg·mL-1,检出限为 4 8× 10 -4 μg·mL-1(3σ) ,RSD小于 3 5 % (n =9) ,并对 45种物质进行了干扰试验。本法成功地应用于药剂中异烟肼含量测定 ,回收率为 98 2 %～ 10 1%。本法与DSC法进行了对照 ,结果满意。     

基于鲁米诺修饰碳糊电极电化学发光异烟肼传感器的研制

通过化学修饰的方法将直接参与电化学发光反应的试剂鲁米诺固定于电极表面,结合醋酸纤维素修饰碳糊电极构建异烟肼电化学发光传感器。在最佳实验条件下,该传感器具有重现性好、选择性高等优点,异烟肼浓度在4.0×10-6—4.0×10-5g/mL范围内与相对电化学发光强度呈线性关系。该方法测定异烟肼的检出限为4.6×10-6g/mL,相对标准偏差为1.2%,相关系数为0.9985,用于样品测定,结果与对照方法吻合。     

火焰原子吸收光谱法测定云南蒙自石榴中微量元素

采用干灰化法消化溶解样品,用原子吸收光谱法连续测定石榴汁中的K、Na、Ca、Mg、Cu、Cr等元素的含量。系统试验了测定条件,实现了同一溶液中测定多种元素,方法简单、快速、准确。测定结果为进一步开发石榴资源提供具有参考价值的数据。     

原子吸收光谱法测定铝锂合金中锂

本文研究了用原子吸收光谱法在笑气－乙炔火焰中测定铝锂合金中锂的最佳条件。其电离干扰可通过加进钾进盐来控制。应用本法测定合金中 锂的含量，获得了满意结果。     

活性炭富集—GFAAS法测定乙烯气中的砷含量

提出一种乙烯气中砷的富集，消解方法，详细地考察了实验条件，用ＧＦＡＡＳ法对乙然气中砷含量进行了测定。相对标准偏差〈４．７％，测定结果与氢化物原子荧光法测定结果相吻合。     

火焰发射法测定冶炼废渣中的铟

采用火焰原子发射光谱法对含铟冶炼废渣中的铟含量进行了测定，对测定条件，干扰因素进行了研究，并与火焰原子吸收法的结果进行了对比。     

高效毛细管电泳内标法与外标法含量测定的比较

比较研究高效毛细管电泳内标法与外标法进行含量测定结果的差异.采用高效毛细管电泳内标法(以二苯胍为内标物)和外标法分别测定三黄泻心汤3种不同剂型中盐酸小檗碱的含量,并进行了比较.外标法测得结果较内标法偏低,高效毛细管电泳内标法比外标法含量测定结果理想.     

两种测定果汁中钾磷含量方法的比较与分析

采用微波消解-等离子体发射光谱法同时测定果汁中K、P含量,优化了实验所需的各种条件。与国家标准方法湿法消解-分光光度法的测定结果作了实验比较,结果表明本实验方法更简单、高效,节省了样品的前处理时间,减小了最终结果的误差。该法灵敏度高、线性范围宽、加标回收率高,结果令人满意。     

HPLC测定双黄连口服液中黄芩苷含量的实验条件优化

对HPLC测定双黄连口服液中黄芩苷含量的实验条件采用正交试验法进行研究,结合直观分析和方差分析综合讨论了色谱条件,寻找出影响HPLC测定双黄连口服液中黄芩苷含量较显著的因子以及色谱条件的最佳化趋势,通过系统设计实验,确定水中冰乙酸的百分含量为影响检测最显著的因子,并优选出最佳色谱条件趋势为:流动相甲醇百分含量为45%,水中冰乙酸百分含量为2%,柱温40℃。     

PMR Determination of Isoniazid

A PMR analytical procedure is described for the quantitation of isoniazid (4-pyridinecarboxylic acid hydrazide) as a drug entity and in tablets dosage form. The method is accurate and precise with a standard deviation of ± 1.51 and ± 1.34 in the bulk drug and in tablets respectively. The results obtained comply with the official standards required by different pharmacopeias. The PMR spectrum, in addition, provides a very specific mean for identification of isoniazid.     

Optical Detection of Some Hydrazine Compounds Based on the Surface Plasmon Resonance Band of Silver Nanoparticles

An indirect colorimetric method is presented for spectrophotometric determination of hydrazine, phenylhydrazine, and isoniazid. Reduction of silver ions to silver nanoparticles (AgNPs) by these analytes as active reducing agents in the presence of polyvinylpyrrolidone (PVP) and also cetyltrimethylammonium chloride (CTAC) as a stabilizer is the basis of the proposed method. The changes in plasmon absorbance of the AgNPs at λ = 415 nm in the presence of PVP were proportional to concentration of hydrazine, phenylhydrazine, and isoniazid in the ranges of 4.0–150.0 µM, 1.0–55.0 µM, and 2.0–30.0 µM, respectively, and the detection limit obtained was 0.79 µM. In the presence of CTAC, the linear ranges were 0.5–10.0 and 10.0–300.0 µM for hydrazine, 1.0–40.0 µM for phenylhydrazine, and 0.2–10.0 and 10.0–90.0 µM for isoniazid, and the detection limit was 0.12 µM. The method has been applied for determination of these analytes in different real samples such as boiler feed water and tablet.     

DERIVATIVE RATIO SPECTROPHOTOMETRY AND DIFFERENTIAL DERIVATIVE SPECTROPHOTOMETRIC DETERMINATION OF ISONIAZID AND PYRIDOXINE HYDROCHLORIDE IN DOSAGE FORMS

Derivative ratio spectrophotometric and differential derivative spectrophotometric methods, for the determination of isoniazid and pyridoxine hydrochloride in pharmaceutical dosage forms (tablets) is described. The first method depends on ratio derivative spectra spectrophotometry where isoniazid and pyridoxine hydrochloride were determined by the measurement of the first derivative ratio spectra of amplitudes (Abs.) at 250.7 and 305.8 nm, respectively. The other method, is based on differential derivative spectrophotometry for the simultaneous determination of isoniazid and pyridoxine hydrochloride in binary mixture without any pre-treatment by measuring the ΔD₁ values.

Both methods showed good linearity, precision and reproducibility. The proposed methods were successfully applied to the pharmaceutical dosage forms containing the above-mentioned drug combination without any interference by the excipients.     

Remote sensing by open-path FTIR spectroscopy. Comparison of different analysis techniques applied to ozone and carbon monoxide detection

The analysis of FTIR spectra is usually carried out by commercial programs that use methods based on classical least-squares (CLS) procedures These procedures are normally appropriate to analyse O-P FTIR spectra, but some occasional discrepancies with standard extractive methods have been observed.In this work, a line-by-line method (SFIT) is additionally used. Our purpose is to explain the sporadic discrepancies observed between CLS and standard extractive method results and to study the capability of both methods (CLS and SFIT) to analyse open-path measurements. This capability has been studied for ozone and carbon monoxide.It has been demonstrated that in CLS-based methods discrepancies are induced by the experimental background reference spectrum. In these cases, SFIT results are in very good agreement with the standard extractive methods.     

5‘—硝基水杨基荧光酮双波长标准加入分光光度法同时测定？…

在０．３－０．９ｍｏｌ／Ｌ硫酸介质中，在溴化十六烷基三甲铵存在下，５’－硝基水杨基荧光酮与锗和钼形成最大吸收波长分别为５１３和５３３ｎｍ的红色配合物，其吸收光谱严重重叠。基于此，本文采用双波长标准加入法对该混合物的显色体系进行了研究，建立了同时分光光度测定锗和钼的新方法，并与等吸收双波长法作了比较。     

Reduction of external noise by building facades: tolerance of standard EN 12354-3

The European standard EN 12354-3 describes a calculation model designed to estimate the reduction of outdoor sound by facades of buildings. The accuracy of the prediction method is not specified in the standard. In this paper, the tolerance of the method is studied by comparing the calculation results to the laboratory and field measurement results of different types of facades. For the weighted sound reduction index, the mean and standard deviation of the difference between the calculated and measured values of nineteen facades is 0.3±0.4 dB in laboratory conditions. In field conditions, the mean and standard deviation of the difference between the calculated and measured values of twelve facades is 3.8±3.8 dB. The tolerance is considerably larger compared to the laboratory measurements because the sound insulation of the single building facade elements have been evaluated based on the empirical estimations and not on measurement results.     

钇,钡,铜超导薄膜的非破坏测定

本文配合阶段性薄膜制备工艺,用有限厚度范围内的转换因子法及模拟薄膜标样的基本参数法,X-射线荧光光谱非破坏测定钇、钡、铜超导薄膜。经我们实验,它们与破坏法ICP相比,A_((Ba/Y))克原子比的平均绝对偏差分别为0.15与0.065,A_((Cu/Y))克原子比的平均绝对偏差分别为0.14与0.088,模拟薄膜标样的基本参数法优于转换因子法。作者还对薄膜试样非破坏测定中的标样选用进行了探讨。     

ICP—AES法同时测定硼铁中的主要成分及杂质

本文研究了用ＩＣＰ－ＡＥＳ法同时测定硼铁中的主要成分Ｂ、Ｆｅ及杂质元素、Ａｌ、Ｍｎ，Ｓｉ的方法。采用碱熔融法分解样品，选Ｃｄ作为内标元素。     

异烟肼乳酸-乙醇酸共聚物磁性微球的制备和体外释放

采用化学共沉法制备磁性Fe3O4纳米粒,再采用W/O/W复乳-溶剂挥干法制备异烟肼乳酸-乙醇酸共聚物[Poly(lactide-co-glycolide),PLGA]磁性微球(INH-PLGA-MMS);电镜考察INH-PLGA-MMS形态、激光粒径分析仪考察粒径分布、磁场计测定磁感应强度、高效液相色谱法(HPLC)测定包封率、载药量及其释放度。结果表明,W/O/W复乳-溶剂挥干法制得的INH-PLGA-MMS外观圆整、表面光滑,平均粒径为3.02μm,磁感应强度为11.403emu/g,平均包封率为62.52%,平均载药量为9.21%,体外释放表明该制剂具有明显的缓释功能,外加振荡磁场可以增加磁性微球中药物的释放。