双轴准直测量调节系统

根据神龙一号和神龙二号两台直线感应加速器的直线性偏差不超过±0.2 mm、角度误差不超过±2″和高程误差不超过±0.2 mm的总体精度要求,分析了影响准直安装精度的主要因素和误差限定值;确定了采用激光跟踪仪、全站仪和水准仪相结合,建立包括大六边形网和小四边形网两个层次的高精度控制测量网;再通过专门设计的串并联精密调节机构,依据合理的准直安装工艺达到准直安装的精度要求。经过在神龙一号直线加速器准直安装的验证,此方法满足精度要求。     

Coverage percentage and coverage rate of different DNA nanostructures grown on a mica substrate

In this study, we simultaneously grew different DNA nanostructures, including 5 helix ribbon (5HR), double-crossover (DX), and double-crossover with single hairpin (DX-SH) lattices, on a mica substrate, and we analyzed the concentration-dependent coverage percentages of 5HR at a fixed concentration of DX ([DX]), DX at a fixed [5HR], and DX-SH with both fixed [5HR] and [DX]. The structural features of the various nanostructures formed through mica-assisted growth annealing were confirmed via atomic force microscopy. To assess the temperature-dependent coverage rates on the given substrates, we introduced two simple models, i.e., the equipartition model (EPM) and the un-equipartition model (UEPM) which provide information on the amount of coverage for each of the given temperature. EPM provides an equal amount of the coverage percentage per temperature change due to its linear nature, but UEPM does not since it has a non-linear nature. The coverage percentages obtained by these models allow us to calculate, as a function of temperature, the coverage rates of 5HR at a fixed [DX] and DX-SH for both fixed [5HR] and [DX]. Although with EPM the coverage rates for the 5HR and DX-SH decreased linearly as the temperature decreased, they decreased non-linearly as the temperature decreased with UEPM.     

Fabrication and tribological properties of super-hydrophobic surfaces based on porous silicon

In the present work, super-hydrophobic surfaces based on porous silicon (PS) were constructed by the self-assembled molecular films and their tribological properties were investigated. A simple chemical etching approach was developed to fabricate PS with the certain rough microstructure surface, which can be observed by the environmental scanning electron microscopy (ESEM). The hydrocarbon and fluorocarbon alkylsilane molecular films were self-assembled on PS, which was confirmed by the X-ray photoelectron spectroscopy (XPS) measurement. In contrast to PS, the alkylsilane molecular films modified PS (mPS) were super-hydrophobic since the apparent water contact angle (CA) exceeded 160°. The tribological properties of PS and the mPS were investigated by a ball-on-disk tribometer during the processes of different sliding velocities and normal loads. The experimental results showed that the alkylsilane molecular films could decrease the friction coefficient. Due to the difference of chain structure and functional groups, the fluorinated alkylsilane films are better candidates for improving the hydrophobicity and lubricating characteristics of PS comparing to the non-fluorinated ones. The carbon chain length of alkylsilane molecules self-assembling on the Si or PS substrates could have little effects on the hydrophobic properties and the tribology performances.     

Surface layer reflective index changes of Au nanoparticle functionalized porous silicon microcavity for DNA detection

A technique is demonstrated to detect DNA hybridization based on surface layer of Au/porous silicon microcavity (Au/PSM) substrate for very small amount of biomolecules. Simulations show that the increase of effective refractive index for the first layer of PSM will cause a blue shift for its reflectance spectrum, and the blue shift becomes less with the increase of refractive index for one more layers. In experiments, such a blue shift of reflectance spectrum of PSM comes from the increase of refractive index by DNA hybridization on the surface. The detection limit of Au/PSM biosensor is 15.15 nM for 19-base pair DNA, which is comparable to that of reported biosensors based on porous silicon (PS). Therefore such an Au/PSM could be very useful to develop simple, rapid and sensitive optical biosensors when the amount of target is very small.     

Stabilization of Pt nanoparticles by single stranded DNA and the binary assembly of Au and Pt nanoparticles without hybridization

The non-specific interaction between single stranded DNA (ssDNA) and 12 nm Pt nanoparticles is investigated in this work. The data show a strong and non-specific interaction between the two which can be exploited for the stabilization of Pt nanoparticles in aqueous solutions. Based on the experimental findings, a non-hybridization based protocol to assemble 17 nm Au and Pt nanoparticles (12 nm cubic and 3.6 nm spherical) by single-stranded DNA was developed. Transmission electron microscopy (TEM) and UV–visible spectroscopy confirmed that Au and Pt nanoparticles could be assembled by the non-specific interaction in an orderly manner. The experimental results also caution against the potential pitfalls in using DNA melting point analysis to infer metal nanoparticle assembly by DNA hybridization.     

Control of surfaces and heterointerfaces of AlGaN/GaN system for sensor devices and their on-chip integration on nanostructures

For successful construction of sensor devices and their future on-chip integration on nanostructures, this paper discusses the present status of understanding and control of surfaces and heterointerfaces of the AlGaN/GaN material system by reviewing a series of works recently carried out by the authors’ group.Leakage currents in Schottky contacts are explained by the authors’ thin surface barrier (TSB) model. An important role is played by oxygen shallow donors in leakage in AlGaN Schottky diodes. A large leakage reduction has been achieved by a novel surface control process for oxygen gettering. An unprecedented high sensitivity has been obtained in AlGaN/GaN Schottky diode hydrogen sensor by applying the surface control process. Liquid-phase AlGaN/GaN sensors having an open-gate HFET structure show a very good pH sensing capability as well as a good sensing capability of polar liquids. Finally, the selective MBE growth of AlGaN/GaN nanowire network is discussed as a basic hardware structure for the on-chip integration of sensors, paying attention to the heterointerface control.     

Quantum Logic Networks for Probabilistic and Controlled Teleportation of Unknown Quantum States

We present simplification schemes for probabilistic and controlled teleportation of the unknown quantum states of both one particle and two particles and construct efficient quantum logic networks for implementing the new schemes by means of the primitive operations consisting of single-qubit gates, two-qubit controlled-not gates, Von Neumann measurement, and classically controlled operations. In these schemes the teleportation are not always successful but with certain probability.     

Molecular devices for high fidelity of DNA replication and gene expression

Certain types of DNA lesions, produced through cellular metabolic processes and also by external environmental stresses, are responsible for the induction of mutations as well as of cancer. Most of these lesions can be eliminated by DNA repair enzymes, and cells carrying the remaining DNA lesions are subjected to apoptosis. The persistence of damaged bases in RNA can cause errors in gene expression, and the cells appear to possess a mechanism which can prevent damaged RNA molecules from entering the translation process. We have investigated these processes for high fidelity of DNA replication and gene expression, by using both biochemical and genetic means. We herein describe (1) the molecular mechanisms for accurate DNA synthesis, (2) mammalian proteins for sanitizing the DNA precursor pool, (3) error avoidance mechanisms for gene expression under oxidative stress, and (4) the roles of DNA repair and apoptosis in the prevention of cancer.     

Quantum Logic Networks for Probabilistic and Controlled Teleportation of Unknown Quantum States

We present simplification schemes for probabilistic and controlled teleportation of the unknown quantum states of both one particle and two particles and construct efficient quantum logic networks for implementing the new schemes by means of the primitive operations consisting of single-qubit gates, two-qubit controlled-not gates, Von Neumann measurement, and classically controlled operations. In these schemes the teleportation are not always successful but with certain probability.     

New developments of the R&;D silicon tracking for linear collider on silicon trackers

The status of the R&D activity achieved so far within the SiLC (silicon tracking for the linear collider) collaboration is reported here. It includes the following items: present status of the collaboration, new developments on sensors, on mechanics (new directions for module construction, large support structure, cooling, and alignment and integration issues), new lab test bench results on electronics and sensors. The perspectives over a period of four years are presented with a detailed test beam schedule and the roadmap including the construction of new mechanical prototypes equipped with front end and readout chips in deep sub-micron CMOS technology are discussed. Combined tests with other sub-detectors are finally addressed. This test beam program is inserted in the framework of the EUDET European project. on behalf of the SiLC R&D Collaboration     

Influence of steps and defects on the dissociative adsorption of molecular hydrogen on silicon surfaces

-4 . Neither the absolute values nor the temperature dependence of adsorption on the terraces are affected by the misorientation. Received: 3 November 1998     

Preliminary thoughts on the data acquisition for the next generation of silicon tracking systems

Preliminary thoughts about the data acquisition system to be developed for the next generation of large area silicon tracker are presented in this paper. This paper describes the set of data delivered by these tracking systems, and the various stages of processing and data flow transmission from the front-end chip sitting on the detector to the latest stage in the data processing. How to best profit from the status of the art technologies is a major goal.      

A solid-state electrochemiluminescence biosensing switch for detection of DNA hybridization based on ferrocene-labeled molecular beacon

A solid-state electrochemiluminescence (ECL) biosensing switch incorporating quenching of ECL of ruthenium(II) tris-(bipyridine) (Ru(bpy)₃²⁺) by ferrocene (Fc) has been successfully developed for DNA hybridization detection. The important issue for this biosensing system is based on the ferrocene-labeled molecular beacon (Fc-MB), i.e. using the special Fc-MB to react with the target DNA and then change its structure, resulting in an ECL intensity change. Under the optimal conditions, the difference of ECL intensity before and after the hybridization reaction (ΔI_ECL) was linearly related to the concentration of the complementary sequence in the range of 10 fM-10 pM and the detection limit was down to 1.0 fM.     

Copper-induced crystallization of sputtered silicon on ZnO:Al substrate and the textured interface for light trapping

Copper-induced crystallization of a-Si on ZnO:Al (AZO) substrate is studied. On Ar sputtered AZO substrate, the optimized crystallite and crystalline ratio of poly-Si are ∼30 nm and ∼71%, respectively. O₂ is also introduced and optimized when preparing AZO substrates. On AZO substrate with O₂/Ar + O₂ = 3%, the crystallite and crystalline ratio of poly-Si are greatly improved, showing ∼40 nm and ∼82%, respectively. Textured AZO is prepared for analyzing the light-trapping efficiency. With 40 s etching in 0.5% HCl, ∼0.7 μm lateral scale and ∼119 nm root mean square roughness is obtained. The scattering property is verified by the flat step over a large angle range in the angular distribution measurement. 660 nm Cu-induced poly-Si on this AZO substrate shows an average reflectivity of ∼17.7%, only 45% of the flat Si, showing a good light-trapping efficiency and a potential use in solar cells.     

Self-assembly of silanated poly(ethylene glycol) on silicon and glass surfaces for improved haemocompatibility

Surface immobilization of poly(ethylene glycol) (PEG) is an effective method to produce a material surface with protein repulsive property. This property could be made permanent by using covalent grafting of the PEG molecules onto material surfaces. In this study, self-assembled monolayers (SAMs) of PEG on silicon-containing materials (silicon chip and glassplate) were obtained through a one-step coating procedure of one kind of silanated PEG molecules made through the reaction between monomethoxy PEG and 3-isocyanatopropyltriethoxysilane. Atomic force microscopy (AFM) and water static contact angle measurement were employed to investigate the surface topography and wettability of the PEGylated material surfaces. The changes in the topography and the water contact angle of the surfaces with time of incubation in PBS solution were also measured. The results revealed that stable and uniform self-assembled monolayers of PEG could be formed on silicon or glass surfaces by simply soaking the substrates in the solution of silanated PEGs. The covalent coupling of PEGs to the substrates was also confirmed. In order to evaluate the stability of the SAMs, blood compatibility of the modified glassplate surface was evaluated by measuring full blood activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT), as well as by scanning electron microscopy (SEM) analysis of the appearance of adherence and denaturation of blood platelets onto the glassplate. The silanated PEGs were shown to have good effect on the protein-repulsion as well as haemocompatibility of the substrates.     

The molecular orientation of DNA bases on H-passivated Si(1 1 1) surfaces investigated by means of near edge X-ray absorption fine structure spectroscopy

Layers of the DNA bases adenine, cytosine, and guanine were deposited onto hydrogen passivated Si(1 1 1) surfaces. The average tilt angles of these molecules with respect to the substrate surface were determined by the angular dependence of the Near Edge X-ray Absorption Fine Structure (NEXAFS) of the carbon K-edge. The interpretation of the NEXAFS spectra was assisted by a semi-empirical approach to the calculation of the π^∗-transition region which employs density functional theory calculations and core level photoemission data.     

Adsorption and surface dynamics of short DNA and LNA oligonucleotides on single-crystal Au(1 1 1) electrode surfaces

We have studied the surface dynamics of a double-strand decanucleotide (HS-10AT^L) with 10 adenine-thymine base pairs linked to a Au(1 1 1)-electrode surface via a hexamethylene thiol linker. The study is based on a combination of voltammetry, interfacial capacitance data, electrochemical in situ scanning tunnelling microscopy, and X-ray photoelectron spectroscopy. The thymine bases of the oligonucleotide are connected to furanoses locked in a C3′-endo configuration called LNA (locked nucleic acid). Hybridization in solution is effected prior to linking to the Au(1 1 1)-surface. The melting point of the linker-free locked decanucleotide, 10AT^L is >63 °C. However, voltammetric reductive desorption of the adsorbed thiol-modified double-strand decanucleotide, HS-10AT^L, gives almost the same charge as single-strand HS-10A, 29 ± 3 and 27 ± 5 μC cm⁻², respectively. In situ STM after HS-10AT^L-immobilization also gives images showing highly ordered domains, virtually indistinguishable from those of immobilized HS-10A. X-ray photoelectron spectroscopy gives an N/P ration of 5.0 for HS-10AT^L in line with the expected value for single-strand HS-10A (5.0).All three sets of data suggest that HS-10AT^L hybridized in solution is significantly dissociated on binding to the Au(1 1 1)-electrode surface. This points to an adsorption mechanism in which a stable high density of Au-S bonds is achieved but at the expense of significant unzipping of the more voluminous duplex form.     

A simple and accurate approach for calculating the vibration spectra of molecules on surfaces: Comparisons to high resolution electron energy loss data for ethylene on silicon

Peak assignment is a complex but important task for analyzing the vibration spectra of surface-bound molecules. Here we describe a simple approach for calculating infrared and Raman spectra for surface-bound molecules using a cluster model approach with quantum capping potentials (QCPs). The utility of the approach is demonstrated by comparisons to the measured high resolution electron energy loss spectra for ethylene on clean silicon. By capping the silicon cluster with QCPs we computed spectra that agree very well with the HREEL spectrum, allowing us to easily assign the experimental peaks. QCPs are similar to effective core potentials, can be used with any ab initio technique and most computational chemistry packages, and their use requires no special expertise.     

A study on interaction of DNA molecules and carbon nanotubes for an effective ejection of the molecules

The ejection of DNA molecules from carbon nanotubes is reported from interaction energy perspectives by molecular dynamics simulations. The critical ejection energy, which is to be applied to a DNA molecule for a successful ejection from a carbon nanotube, is investigated based on a study on the friction and binding energy between the DNA molecule and the tube. An effective ejection is realized by subjecting a kinetic energy on the DNA molecule that is larger than the solved critical ejection energy. In addition, the relationship between ejection energies and sizes of DNA molecules and carbon nanotubes is investigated.     

The frequency characteristics analysis of series photodetector frequency circuit system based on circuit parameters of quartz crystal and its application for bacterial DNA identification

The circuit parameters of quartz crystal were employed for frequency sensitivity analysis of series photodetector frequency circuit system. The influence of circuit parameters of quartz crystal on the oscillation frequency and response sensitivity were theoretically derived and experimentally verified. On the basis of optimal circuit parameters, the DNA probe detection limit 2 pmol/L can be measured by 49.4 MHz sensor system. In comparison with the conventional fluorescence technique, the frequency method showed that the detection limits of DNA probe AH642 with Cy5 fluorescence dye and DNA probe VA180 with Cy5 fluorescence dye were lower than the conventional fluorescence technique by 2–3 orders; meanwhile, through the feature of probe uniqueness, Aeromonas hydrophila DNA and fluorescence probe AH642-Cy5 can be successfully judged for hybridization reaction. Moreover, Vibro alginolyticus DNA and fluorescence probe VA180-Cy5 can be successfully judged for hybridization reaction.