电化学发光法测定盐酸普鲁卡因

基于盐酸普鲁卡因对鲁米诺在中性介质中铂电极上电化学发光的催化增敏作用 ,建立了测定盐酸普鲁卡因电化学发光新方法。电化学发光强度与盐酸普鲁卡因质量浓度在 4 .0× 1 0 -7～6 .0× 1 0 -6g mL范围内有良好的线性关系 ,检测限为 2 .0× 1 0 -7g mL,相对标准偏差为 4 .4 %。该方法已用于针剂中盐酸普鲁卡因的测定     

流动注射电化学发光分析法测定氨苄西林

基于氨苄西林对鲁米诺在铂电极上弱的电氧化发光信号的强增敏作用与流动注射技术的结合,建立了一种测定氨苄西林的电化学发光分析新方法。该法测定氨苄西林的检出限为5.0×10-8g mL,线性范围为8.0×10-8～5.0×10-5g mL,相对标准偏差为2.0%(n=11)。已成功地用于样品中氨苄西林的测定。     

流动注射电化学发光分析法测定诺氟沙星的研究

基于诺氟沙星对鲁米诺在铂电极上弱的电氧化发光信号的强增敏作用与流动注射技术的结合 ,建立了一种测定诺氟沙星的电化学发光分析新方法。该法测定诺氟沙星的检出限为 4 .0× 10 -6g/L ;线性范围为 1.0× 10 -5～ 0 .2g/L ;相对标准偏差为 1.2 % (n =11)。该法简单、快速、灵敏 ,已成功地用于药物制剂和尿样中诺氟沙星的测定。     

流动注射电化学发光分析法测定间苯三酚

基于间苯三酚对鲁米诺在铂电极上弱的电氧化发光信号的强增敏作用与流动注射技术的结合 ,建立了一种测定间苯三酚的电化学发光新方法。该法测定间苯三酚的检出限为 1.2× 10 - 4g L ;线性范围为 4 .0× 10 - 4～ 4 .0× 10 - 2 g L ;相对标准偏差为 4 .7%。     

半胱氨酸对鲁米诺电化学发光的增敏作用

在pH 7.0~13.0溶液中,半胱氨酸对鲁米诺的电化学发光有敏化作用,电化学发光强度随溶液中半胱氨酸浓度线性增强。在pH 8.0时其线性范围为2.0×10-8~4.5×10-5mol/L。本法的重现性和稳定性较好,为半胱氨酸的测定提供了一种新的方法。     

流动注射电化学发光分析法测定左旋多巴的研究

基于左旋多巴对鲁米诺在铂电极上弱的电氧化发光信号的强增敏作用与流动注射技术的结合,建立了一种测定左旋多巴的电化学发光分析新方法。该法测定左旋多巴的检出限为2.0×10-10g/mL,线性范围为4.0×10-10~2.0×10-6g/mL,相对标准偏差为2.0%(n=11)。该法简单、快速、灵敏,已成功地用于样品中左旋多巴的测定。     

流动注射电化学发光分析法测定甲磺酸培氟沙星

甲磺酸培氟沙星为第三代喹诺酮类抗菌药,疗效好,抗菌谱更广,且对青霉素、头孢菌素、氨基苷类耐药菌株均有效,在临床上被广泛使用。本文发现,在Na2CO3-NaHCO3碱性溶液中,1．40V电解电位下,鲁米诺在铂电极上的弱电化学发光信号可以被甲磺酸培氟沙星强烈地增敏,据此建立了甲磺酸培氟沙星的流动注射电化学发光分析方法。     

流动注射抑制电化学发光法测定维生素C

将在线恒电流电解产生ClO-与Luminol构成了较强的化学发光体系,基于维生素C对该化学发光体系有强抑制作用,结合流动注射技术,建立了测定维生素C的流动注射抑制电化学发光新方法.该方法在维生素C的浓度为2.0×10-10～1.5×10-8 mmol/mL之间分段回归,呈良好的线性,检出限达到5.0×10-11 mmol/mL.     

流动注射电化学发光法测定头孢氨苄

根据在强酸性介质中初生态Mn(Ⅲ)直接氧化头孢氨苄产生强化学发光,将在线恒电流电解产生Mn(Ⅲ)与流动注射技术结合,建立了流动注射电化学发光测定头孢氨苄的新方法。在优化的实验条件下,测定头孢氨苄的线性范围为1.0×10-7~6.0×10-5g/mL,检出限为5×10-8g/mL,相对标准偏差为1.8%(c=5.0×10-6g/mL,n=11)。方法简便快速,用于头孢氨苄胶囊中头孢氨苄含量的测定,结果满意。     

流动注射电化学发光法测定盐酸麻黄碱

基于盐酸麻黄碱(EP)对三联吡啶合钌(Ru(bpy)32 )电化学发光(ECL)的增敏作用,建立了流动注射ECL检测EP的新方法,并将其应用于EP的测定。结果表明,在pH为10.0的0.1 mol/L Na2B4O7-NaOH介质中,在电位1.10 V下进行恒电位电解,Ru(bpy)32 的浓度为1.0×10-4mol/L时,EP对ECL的增敏效果最好。在优化的条件下,测定EP的线性范围为2.40~24.0μg/mL(r=0.9995),检出限为2.00μg/mL,相对标准偏差(RSD)小于1.6%(n=10),加标回收率为97.0%~105%。     

Flow injection chemiluminescence determination of isoniazid using luminol and silver nanoparticles

The effect of silver colloidal nanoparticles (AgNPs) on the luminol–isoniazid system was investigated. It was found that AgNPs could act as a nanocatalyst on the luminol–isoniazid system to generate chemiluminescence (CL). The CL emission spectrum of the luminol–isoniazid–AgNPs system showed a peak with a maximum at 425 nm. It was suggested that the luminophor species was the excited state 3-aminophthalate. The reduction of dissolved O₂ to H₂O₂ by isoniazid and decomposition of H₂O₂ to the oxygen-related radicals were attributed to the catalytic effect of AgNPs. Under optimized conditions, the CL signal intensity was linear with the isoniazid concentration in the range of 10–1000 ng mL^{− 1}, with the correlation coefficient of 0.9996. The limit of detection was 2.7 ng mL^{− 1} isoniazid. The relative standard deviations for seven repeated measurements of 60 and 200 ng mL^{− 1} isoniazid were 1.4 and 2.4%, respectively. The effect of potent interfering compounds on the CL signal intensity of the proposed luminol–isoniazid–AgNPs system was investigated. The proposed method was successfully applied to the determination of isoniazid in a pharmaceutical sample.     

流动注射化学发光增强法测定巯嘌呤的应用研究

试验发现6-巯基嘌呤对Luminol-H2O2-OH-体系的化学发光有较强的增敏作用。据此建立了一种流动注射化学发光增强法测定6-巯基嘌呤的新方法。该法测定6-巯基嘌呤的检出限为7.5×10-9mol/L,线性范围为2.0×10-8~2.5×10-6mol/L,对6.0×10-7mol/L的6-巯基嘌呤测定的相对标准偏差为1.6%(n=11),已用于合成样中6-巯基嘌呤的测定。     

流动注射化学发光分析法测定利血平

研究发现在碱性介质中,利血平对Cr3 催化的Luminol-H2O2体系的化学发光有较强的抑制作用,据此建立了流动注射化学发光抑制法测定痕量利血平的新方法。该法线性范围宽,发光信号的降低值(ΔI)与利血平的质量浓度在1.0×10-8~2.0×10-5g/mL范围内呈良好的线性关系;检出限(3σ)为7.0×10-9g/mL;对于2.0~10-6g/mL利血平进行11次测定,相对标准偏差为1.8%。已用于针剂中利血平的测定。通过对化学发光光谱、紫外吸收光谱的研究,对机理进行了初步探讨。     

Flow injection chemiluminescence analysis of phenolic compounds using the NCS-luminol system

A flow injection system coupled with two simple and sensitive chemiluminescence (CL) methods is described for the determination of some phenolic compounds. The methods are based on the inhibition effects of the investigated phenols on the CL signal intensities of N-chlorosuccinimide-KI-luminol (NCS-KI-luminol) and NCS-luminol systems. The influences of the chemical and hydrodynamic parameters on the decrease in CL signal intensities of NCS-KI-luminol and NCS-luminol systems for hydroquinone, catechol, and resorcinol, serving as the model compounds of analyte, were studied in the flow injection mode of analysis. Under the selected conditions, the proposed CL systems were used for the determination of some phenolic compound and analytical characteristics of the systems including calibration equation, correlation coefficient, linear dynamic range, limit of detection, and sample throughput. The limits of detection for hydroquinone, catechol, and resorcinol were 0.002, 0.01, and 0.3 μM using the NCS-KI-luminol system; for the NCS-luminol system these were 0.01, 0.17, and 1.6 μM, respectively. The relative standard deviation for 10 repeated measurements of 0.04, 0.06, and 1 μM of hydroquinone, catechol, and resorcinol were 1.9, 1.4, and 2.0%, respectively, with the NCS-KI-luminol system; for 0.2, 0.5, and 4 μM of hydroquinone, catechol, and resorcinol these were 2.6, 2.2, and 3.7%, respectively, using the NCS-luminol system. The method was applied to the determination of catechol in known environmental water samples with a relative error of less than 6%. A possible reaction mechanism of the proposed CL system is discussed briefly.      

Determination of cysteine in a pharmaceutical formulation by flow injection analysis with a chemiluminescence detector

A simple and convenient flow injection-chemiluminescence (FI-CL) method for the determination of cysteine is reported, based on a fast and strong CL in a basic luminol-cysteine-NaIO₄ solution. The linear range was 1.0×10⁻⁸ to 1.0×10⁻⁶ M with a detection limit (3s) of 5×10⁻⁹ M, which was 100 times more sensitive than previously reported CL methods. Singlet oxygen, hydroxyl radical and hydrogen peroxide were suggested to be produced in this reaction and were responsible for the CL of cysteine. This simple method has been successfully applied for the determination of cysteine in a pharmaceutical formulation.     

流动注射阻抑化学发光法测定己烯雌酚

基于在碱性介质中,己烯雌酚对N-溴代丁二酰亚胺-鲁米诺化学发光体系的阻抑作用,建立了测定己烯雌酚的流动注射化学发光分析新方法,探讨并优化了流动注射化学发光的分析条件。该方法测定己烯雌酚的线性范围为1.5×10-8~2.6×10-7g/mL,检出限为5.0×10-9g/mL,对1.0×10-7g/mL的己烯雌酚标准溶液进行11次测定,相对标准偏差为2.6%。并应用于饲料、己烯雌酚片剂中的己烯雌酚的测定。     

鲁米诺-铁氰化钾化学发光体系测定左旋多巴

铁氰化钾在碱性条件能氧化鲁米诺而发光,左旋多巴能大大增强该发光强度。基于此现象并结合流动注射技术,建立起一种直接测定左旋多巴的流动注射化学发光分析法。方法检出限为1.2×10-10g mL,左旋多巴质量浓度在5 0×10-10～5.0×10-8g mL范围内与发光强度呈良好的线性关系。相对标准偏差为2.9%(左旋多巴5.0×10-9g mL,n=11)。该方法可用于片剂中左旋多巴量的测定。     

流动注射协同化学发光法测定盐酸多巴胺

基于在碱性介质中,盐酸多巴胺和单宁协同促进KIO4氧化鲁米诺反应而产生发光,建立了流动注射化学发光法测定盐酸多巴胺的新方法。该方法测定盐酸多巴胺的线性范围为2.0×10-10～6.0×10-8g/mL,检出限为1.17×10-10g/mL,对于8.0×10-10g/mL盐酸多巴胺测定11次的相对标准偏差为1.92%。方法可应用于盐酸多巴胺注射液和尿样中盐酸多巴胺的测定。     

流动注射化学发光法测定茶饮料中的茶多酚

在碱性条件下,茶多酚对鲁米诺-KMnO4化学发光体系具有较强的抑制作用,据此建立了茶多酚的流动注射化学发光测定法。该法的化学发光抑制值ΔICL与茶多酚质量浓度在2.0×10-6~5.0×10-4mg/mL范围内,呈现出良好的线性关系,检出限为1.2×10-7mg/mL,对1.0×10-5mg/mL茶多酚测定的相对标准偏差为2.9%(n=11)。用于茶饮料中茶多酚的测定。