共查询到19条相似文献,搜索用时 78 毫秒
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氧化还原蛋白质在模拟生物膜修饰电极上的直接电化学 总被引:8,自引:0,他引:8
评述了氧化还原蛋白在模拟生物膜这种新型的化学修饰电极上的直接电化学研究的进展。对蛋白质在表面活性剂薄膜电极和多层复合薄膜电极上的电化学行为、模拟生物膜的超分子结构以及蛋白质在该类薄膜修饰电极上对不同底物的电催化性质进行了较详细的介绍。 相似文献
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蛋白质的翻译后修饰对于生命体执行正常的生理功能具有十分重要的作用,是蛋白质科学研究的重要内容.目前研究蛋白质修饰的方法主要有质谱法、亲和层析等,然而由于蛋白质修饰研究的复杂性,迫切需要发掘新的技术手段.电化学方法理论成熟、应用广泛,在生命科学许多领域发挥着越来越重要的作用.蛋白质的体外修饰必将导致蛋白质特定位点基团的变化,可以利用巧妙设计的电化学方法予以表征和分析,以期探明修饰对蛋白质结构和功能的影响.此外,又可以利用电化学定量分析的独特优势快速准确地测定蛋白质修饰中涉及的相关酶活.正因为如此,蛋白质体外修饰的电化学研究已引起越来越多的关注.本文以作者课题组近期研究工作为主,结合国内外同行的相关代表性工作,介绍电化学方法在蛋白质修饰方面的近期研究进展,并探讨了今后的发展方向和趋势. 相似文献
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介孔分子筛上的蛋白质直接电化学 总被引:2,自引:0,他引:2
将介孔分子筛用于不同含血红素蛋白质的直接电子传递研究,分别研究了辣根过氧化物酶、血红蛋白和肌红蛋白在六方介孔硅(HMS)上的直接电化学,探讨了介孔分子筛与这些蛋白质间的相互作用,构建了过氧化氢和亚硝酸根的新型的生物传感器. 这些工作扩展了HMS在蛋白质固定、直接电子传递研究和无试剂生物传感器制备方面的应用. 相似文献
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双层类脂膜及其在电化学生物传感器中的应用 总被引:11,自引:0,他引:11
详细评述了各种双层类脂膜包括传统的双层类脂膜(BLM)、固体载体支撑的自组双层类脂膜(s-BLM)、固体载体支撑的混合双层类脂膜(e-BLM)的制备方法和特性,比较了其优缺点。介绍了双层类脂膜在电化学生物传感器中的应用,并展望了发展前景。 相似文献
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本文对生物大分子电化学行为这一新兴研究领域作了概述,介绍了其研究意义和重要性,主要讨论了血红素蛋白质的直接电化学行为,电极表面电子传递过程,目前的研究概况及已经取得了成果,引用参考文献97篇。 相似文献
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氧化还原蛋白质电化学研究* 总被引:10,自引:0,他引:10
研究氧化还原蛋白质与电极之间的电子传递过程不仅为理解代谢过程提供有价值的信息,而且为制备生物传感器奠定基础。本文从蛋白质修饰电极、蛋白质在电极表面的定向固定及蛋白质人工改造三方面,评述了近年来氧化还原蛋白质电化学研究的进展,并提出了今后可能的研究方向。 相似文献
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辅酶Q在CPT自组装修饰电极上的电化学行为及其分析应用 总被引:1,自引:0,他引:1
采用自组装方法制备了卡托普利(CPT)修饰电化学传感器,并对其在金电极表面形成的单分子膜进行了表征。循环伏安试验结果表明,辅酶Q在CPT修饰电极上能发生准可逆的电化学反应。该传感器对辅酶Q测定具有良好的响应,测定范围为5.0×10-6~6.0×10-5mol.L-1,检出限为2.0×10-6mol.L-1,异相电子传递速率常数ks为5.4×10-3cm.s-1。对CPT修饰膜的稳定性进行考察,讨论辅酶Q发生电化学反应的异相电子传递速率常数ks及其影响机制。 相似文献
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《Electroanalysis》2004,16(15):1193-1204
The knowledge of the redox chemistry of nucleic acids (NA) is of paramount importance in cancer and aging research. Charge migration through DNA is also involved in biologically relevant functions such as DNA damage and repair. In the first part of this article the main aspects of the electrochemistry of nucleic acids at solid electrodes are revised, including redox processes, photoelectroactivity and electrical conductivity. In the second part, an overview of its applications is presented. Methods for electrochemical detection of NA, NA‐based biosensors for detection of nonnucleic acid molecules, studies on the nature and dynamics of interactions and structural conformations of NA, are some applications that take advantage of NA electrochemistry at solid electrodes. 相似文献
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Xinyi Zhang Dr. Dan Li Dr. Laure Bourgeois Dr. Huanting Wang Dr. Paul A. Webley Dr. 《Chemphyschem》2009,10(2):436-441
Nanochannel alumina templates are used as templates for fabrication of porous gold nanowire arrays by a direct electrodeposition method. After modification with glucose oxidase, a porous gold nanowire‐array electrode is shown to be an excellent electrochemical biosensor for the detection of glucose. The picture shows an SEM image of a nanowire array after removal of the alumina template by acid dissolution.
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Eva Alvarez de Eulate Shane O'Sullivan Sharon Fletcher Prof. Dr. Philip Newsholme Prof. Dr. Damien W. M. Arrigan 《化学:亚洲杂志》2013,8(9):2096-2101
The behavior of proteins and polypeptides at electrified aqueous–organic interfaces is of benefit in label‐free detection strategies. In this work, rat amylin (or islet amyloid polypeptide) was studied at the interface formed between aqueous liquid and gelled organic phases. Amylin is a polypeptide that is co‐secreted with insulin from islet beta‐cells and is implicated in fibril formation. In this study, rat amylin was used, which does not undergo aggregation. The polypeptide underwent an interfacial transfer process, from water to the gelled organic phase, under applied potential stimulation. Cyclic voltammetry revealed steady‐state forward and peak‐shaped reverse voltammograms, which were consistent with diffusion‐controlled water‐to‐organic transfer and thin‐film stripping or desorptive back‐transfer. The diffusion‐controlled forward current was greater when amylin was present in an acidic aqueous phase than when it was present in an aqueous phase at physiological pH; this reflects the greater charge on the polypeptide under acidic conditions. The amylin transfer current was concentration dependent over the range 2–10 μM , at both acidic and physiological pH. At physiological pH, amylin was selectively detected in the presence of a protein mixture, which illustrated the bioanalytical possibilities for this electrochemical behavior. 相似文献
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对1991~2008年间各种纳米材料,包括纳米金属粒子、纳米无机材料、纳米碳材料等在蛋白质直接电化学中的应用进行了综述,引用文献58篇。 相似文献
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Taek Dong Chung 《Journal of inclusion phenomena and macrocyclic chemistry》1998,32(2-3):179-193
The electrochemistry of calixarene as a redox-dependent ionophore and its structural dependence are described. One or more redox-centers such as quinone, ferrocene, cobaltocenium and ruthenium bipyridine moieties have been introduced into the calixarene frame of the lower or upper rim. Although the electrochemical behavior depends mainly on the inherent redox property of these electrochemically active groups, the structural effect and solvent also play important roles, especially, in the presence of charged guests. When cationic species such as metal ions and ammonium ion are added to a quinone-functionalized calixarene solution, electron transfer to quinone is enhanced by the electrostatic effect or the formation of hydrogen bonds. In addition to redox-active hosts for voltammetric use, a number of calixarenes with novel structures have been developed as ionophores for potentiometric analysis and found to be successful for some target ions. In terms of Na+, Cs+ and Ca2+ selective ionophores for ion-selective electrodes, calixarenes are found to be excellent compared to crown ether derivatives or cryptands. Calixarenes can be also utilized to construct chemically modified electrodes, which are sensitive to gas species and biologically important compounds. The sophisticated design and synthesis of calixarenes are essential to specific potential applications to diverse fields. 相似文献
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0IntroductionStudies of direct electrochemistry of proteins orenzymes at electrodes can serve as a basis for build-ing electrochemical biosensors,enzymatic bioreactors,and biomedical devices[1].This approach simplifiessuch devices without using mediators and is of partic-ular significance for fabricating the third generationbiosensors[2].For example,if a protein or enzyme im-mobilized on electrode surface is capable of directelectron transfer without loss of bioactivities,it can beused in the … 相似文献
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Jing Zheng Jing‐Li Xu Tang‐Bo He Jin Jia‐Ling Wang Wen‐Qian Zhang Yu‐Xiao Hu Pin‐Gang He Yu‐Zhi Fang 《Electroanalysis》2013,25(9):2159-2165
A novel magnetic ordered mesoporous carbon composite was prepared. Electrochemical measurements showed that the ordered mesoporous carbon composite provided an excellent matrix for the co‐adsorption of horseradish peroxidase (HRP). HRP could be separated and collected by the application of a magnetic field and its direct electron‐transfer could be achieved in the solution, not on the electrode thereby preventing the degradation of the enzyme. The cyclic voltammetric experimental results of HRP indicated that HRP displayed a pair of stable peaks with a formal potential of ?0.306 V in PBS. The resulting biosensor exhibited fast amperometric response to hydrogen peroxide. 相似文献