A Coarse Estimation of Cell Size Region from a Mesoscopic Stochastic Cell Cycle Model

Based on a deterministic cell cycle model of fission yeast, the effects of the finite cell size on the cell cycle regulation in wee1- cdc25△ double mutant type are numerically studied by using of the chemical Langevin equations. It is found that at a certain region of cell size, our numerical results from the chemical Langevin equations are in good qualitative agreement with the experimental observations. The two resettings to the G2 phase from early stages of mitosis can be induced under the moderate cell size. The quantized cycle times can be observed during such a cell size region. Therefore, a coarse estimation of cell size is obtained from the mesoscopic stochastic cell cycle model.     

P53及其相关蛋白对X射线照射肝癌细胞周期的调节

X射线照射人肝癌细胞HepG2， 照射后细胞存活随照射剂量增大明显下降。 流式细胞术分析， 不同剂量组照射后24 h均发生G2期阻滞。 照射后不同时间组的细胞周期分布也有不同， 照射后12 h， 有显著的S期延迟。 Western Blot 显示照射后24 h P53， MDM2， P21蛋白表达上升， 并有时间效应: P53在照射后24 h之内始终维持较高表达, MDM2和P21分别在照射后6和12 h的表达最高。 X射线照射通过影响P53及其相关蛋白的表达影响细胞周期。 HepG2 cells were irradiated with X ray at the doses of 0, 1.0, 2.0, 4.0 or 8.0 Gy and separately maintained in DMEM at 37 ℃ for 0, 6, 12 or 24 h. Colony forming assay showed that cell survival decreased with the irradiation dose increasing. Cell cycle was detected by FACS, the arrest of S phase was found after 12 h irradiation and arrest of G2 phase took place at 24 h after all irradiation doses, which suggested that cell cycle distribution was different in groups gathered after different maintaining time. The results of Western blotting showed that the expression of P53, MDM2 and P21 increased more after irradiation than the control. The expression of P53 remained high at 24 h after irradiation, while the levels of MDM2 or P21 arrived at the highest at 6 h or 12 h after irradiation respectively. The expressions of P21 after irradiation were in corresponding with the cell cycle distribution in the groups of different maintaining time. In conclusion, irradiation change the distribution of cell cycle by effecting the expression of P53 and its related proteins.     

Effects of non-Gaussian noise on the dynamical properties of a logistic system

The dynamical properties of a tumor cell growth system described by the logistic system with coupling between non- Gaussian and Gaussian noise terms are investigated. The effects of the nonextensive index q on the stationary properties and the transient properties are discussed, respectively. The results show that the nonextensive index q can induce the tumor cell numbers to decrease greatly in the case of q 〉 1. Moreover, the switch from the steady stable state to the extinct state is speeded up as the increases of q, and the tumor cell numbers can be more obviously restrained for a large value of q. The numerical results are found to be in basic agreement with the theoretical predictions.     

Radiation effects on MOS and bipolar devices by 8 MeV protons, 60 MeV Br ions and 1 MeV electrons

The radiation effects of the metal-oxide-semiconductor (MOS) and the bipolar devices are characterised using 8~MeV protons, 60~MeV Br ions and 1~MeV electrons. Key parameters are measured {\it in-situ} and compared for the devices. The ionising and nonionising energy losses of incident particles are calculated using the Geant4 and the stopping and range of ions in matter code. The results of the experiment and energy loss calculation for different particles show that different incident particles may give different contribution to MOS and bipolar devices. The irradiation particles, which cause larger displacement dose within the same chip depth of bipolar devices at a given total dose, would generate more severe damage to the voltage parameters of the bipolar devices. On the contrary, the irradiation particles, which cause larger ionising damage in the gate oxide, would generate more severe damage to MOS devices. In this investigation, we attempt to analyse the sensitivity to radiation damage of the different parameter of the MOS and bipolar devices by comparing the irradiation experimental data and the calculated results using Geant4 and SRIM code.     

Evaluation of Influence of Multiple Scattering Effect in Light-Scattering-Based Applications

The extinction cross sections of a system containing two particles are calculated by the T-matrix method, and the results are compared with those of two single particles with single-scattering approximation. The necessity of the correction of the refractive indices of water and polystyrene for different incident wavelengths is particularly addressed in the calculation. By this means, the volume fractions allowed for certain accuracy requirements of single-scattering approximation in the light scattering experiment can be evaluated. The volume fractions calculated with corrected refractive indices are compared with those obtained with fixed refractive indices which have been rather commonly used, showing that fixed refractive indices may cause significant error in evaluating multiple scattering effect. The results also give a simple criterion for selecting the incident wavelength and particle size to avoid the ＇blind zone＇ in the turbidity measurement, where the turbidity change is insensitive to aggregation of two particles.     

Bifurcation and chaos analysis for aeroelastic airfoil with freeplay structural nonlinearity in pitch

The dynamics character of a two degree-of-freedom aeroelastic airfoil with combined freeplay and cubic stiffness nonlinearities in pitch submitted to supersonic and hypersonic flow has been gaining significant attention. The Poincaré mapping method and Floquet theory are adopted to analyse the limit cycle oscillation flutter and chaotic motion of this system. The result shows that the limit cycle oscillation flutter can be accurately predicted by the Floquet multiplier. The phase trajectories of both the pitch and plunge motion are obtained and the results show that the plunge motion is much more complex than the pitch motion. It is also proved that initial conditions have important influences on the dynamics character of the airfoil system. In a certain range of airspeed and with the same system parameters, the stable limit cycle oscillation, chaotic and multi-periodic motions can be detected under different initial conditions. The figure of the Poincaré section also approves the previous conclusion.     

Extinction Effects of Multiplicative Non-Gaussian L′evy Noise in a Tumor Growth System with Immunization

The extinction phenomenon induced by multiplicative non-Gaussian Levy noise in a tumor growth model with immune response is discussed. Under the influence of the stochastic immune rate, the model is analyzed in terms of a stochastic differential equation with multiplicative noise. By means of the theory of the infinitesimal generator of Hunt processes, the escape probability, which is used to measure the noise-induced extinction probability of tumor cells, is explicitly expressed as a function of initial tumor cell density, stability index and noise intensity. Based on the numerical calculations, it is found that for different initial densities of tumor cells, noise parameters play opposite roles on the escape probability. The optimally selected values of the multiplicative noise intensity and the stability index are found to maximize the escape probability.     

Dynamical behaviors of a system with switches between the Rssler oscillator and Chua circuits

The behaviors of a system that alternates between the R¨ossler oscillator and Chua＇s circuit is investigated to explore the influence of the switches on the dynamical evolution.Switches related to the state variables are introduced,upon which a typical switching dynamical model is established.Bifurcation sets of the subsystems are derived via analysis of the related equilibrium points,which divide the parameters into several regions corresponding to different types of attractors.The dynamics behave typically in period orbits with the variation of the parameters.The focus/cycle periodic switching phenomenon is explored in detail to present the mechanism of the movement.The period-doubling bifurcation to chaos can be observed via the doubling increase of the turning points related to the switches.Furthermore,period-decreasing sequences have been obtained,which can be explained by the variation of the eigenvalues associated with the equilibrium points of the subsystems.     

Comparisons of ZnO codoped by group ⅢA elements （Al,Ga, In） and N： a first-principle study

The electronic structures and effective masses of the N mono-doped and Al N, Ga-N, In-N codoped ZnO system have been calculated by a first-principle method, and comparisons among different doping cases are made. According to the results, the impurity states in the codoping cases are more delocalised compared to the N mono-doping case, which means a better conductive behaviour can be obtained by codoping. Besides, compared to the Al-N and Ga-N codoping cases, the hole effective mass of In-N codoped system is much smaller, indicating the p-type conductivity can be more enhanced by In N codoping     

Dynamical behaviors of a system with switches between the Rssler oscillator and Chua circuits

The behaviors of a system that alternates between the R¨ossler oscillator and Chua’s circuit is investigated to explore the influence of the switches on the dynamical evolution.Switches related to the state variables are introduced,upon which a typical switching dynamical model is established.Bifurcation sets of the subsystems are derived via analysis of the related equilibrium points,which divide the parameters into several regions corresponding to different types of attractors.The dynamics behave typically in period orbits with the variation of the parameters.The focus/cycle periodic switching phenomenon is explored in detail to present the mechanism of the movement.The period-doubling bifurcation to chaos can be observed via the doubling increase of the turning points related to the switches.Furthermore,period-decreasing sequences have been obtained,which can be explained by the variation of the eigenvalues associated with the equilibrium points of the subsystems.     

In vitro three-dimensional cancer metastasis modeling:Past,present, and future

Metastasis is the leading cause of most cancer deaths, as opposed to dysregulated cell growth of the primary tumor.Molecular mechanisms of metastasis have been studied for decades and the findings have evolved our understanding of the progression of malignancy. However, most of the molecular mechanisms fail to address the causes of cancer and its evolutionary origin, demonstrating an inability to find a solution for complete cure of cancer. After being a neglected area of tumor biology for quite some time, recently several studies have focused on the impact of the tumor microenvironment on cancer growth. The importance of the tumor microenvironment is gradually gaining attention, particularly from the perspective of biophysics. In vitro three-dimensional(3-D) metastatic models are an indispensable platform for investigating the tumor microenvironment, as they mimic the in vivo tumor tissue. In 3-D metastatic in vitro models, static factors such as the mechanical properties, biochemical factors, as well as dynamic factors such as cell–cell, cell–ECM interactions, and fluid shear stress can be studied quantitatively. With increasing focus on basic cancer research and drug development, the in vitro 3-D models offer unique advantages in fundamental and clinical biomedical studies.     

Influence of temperature and alloying elements on the threshold displacement energies in concentrated Ni–Fe–Cr alloys

Concentrated solid-solution alloys(CSAs) have demonstrated promising irradiation resistance depending on their compositions. Under irradiation, various defects can be produced. One of the most important parameters characterizing the defect production and the resulting defect number is the threshold displacement energies(E_d). In this work, we report the results of E_dvalues in a series of Ni–Fe–Cr concentrated solid solution alloys through molecular dynamics(MD)simulations. Based on several different empirical potentials, we show that the differences in the E_dvalues and its angular dependence are mainly due to the stiffness of the potential in the intermediate regime. The influences of different alloying elements and temperatures on E_dvalues in different CSAs are further evaluated by calculating the defect production probabilities. Our results suggest a limited influence of alloying elements and temperature on E_dvalues in concentrated alloys. Finally, we discuss the relationship between the primary damage and E_dvalues in different alloys. Overall, this work presents a thorough study on the E_dvalues in concentrated alloys, including the influence of empirical potentials,their angular dependence, temperature dependence, and effects on primary defect production.     

Hydrogen passivation of multi—crystalline silicon solar cells

The effects of hydrogen passivation on multi-crystalline silicon (mc-Si) solar cells are reported in this paper. Hydrogen plasma was generated by means of ac glow discharge in a hydrogen atmosphere. Hydrogen passivation was carried out with three different groups of mc-Si solar cells after finishing contacts. The experimental results demonstrated that the photovoltaic performances of the solar cell samples have been improved after hydrogen plasma treatment, with a relative increase in conversion efficiency up to 10.6\%. A calculation modelling has been performed to interpret the experimental results using the model for analysis of microelectronic and photonic structures developed at Pennsylvania State University.     

Comparisons of ZnO codoped by group IIIA elements (Al, Ga, In) and N: a first-principle study

The electronic structures and effective masses of the N mono-doped and Al-N,Ga-N,In-N codoped ZnO system have been calculated by a first-principle method,and comparisons among different doping cases are made.According to the results,the impurity states in the codoping cases are more delocalised compared to the N mono-doping case,which means a better conductive behaviour can be obtained by codoping.Besides,compared to the Al-N and Ga-N codoping cases,the hole effective mass of In-N codoped system is much smaller,indicating the p-type conductivity can be more enhanced by In-N codoping.     

Short-Wavelength Recording Properties of TeOx Thin Films

We prepare TeOx thin films by vacuum evaporation of TeO2 powder.It is found that the as-deposited TeOx films can represent a two-component system comprising crystalline tellurium particles dispersed in an amorphous TeO2 matrix.Results of the static recording test show that the TeOx films have good writing sensitivity for shortwavelength laser beam (514.5nm).Primary results of the dynamic recording test at 514.5 nm are also reported.The carrier-to-noise ratio of 30dB is obtained for the disc using a TeOx film as the recording medium.Atomic force microscopy is used to study the microstructure of recorded marks.Micro-area morphology images show that the marks are mechanically deformed,and depressions and bulges have been imaged in the recorded marks,resulting in the scattering of the reading laser beam.The analytical results of transmission electron microscopy show that there is not obvious difference between the phase states of the tellurium particles before and after laser irradiation.Recording mechanisms of the TeOx thin films are discussed based on the experimental results.     

Photoacoustic measurement of the thermal property of condensed matter using a strong light-absorbing surface layer

By using a strong light-absorbing surface layer and front-surfaceillumination with a low power He-Ne laser(6mW),photoacousticmeasurements of the thermal effusivities of materials have been made,based on the photoacoustic theory,derived in this paper,of condensedmatter with a strong light-absorbing surface layer.This method can elimi-nate the stray light,give full play to the power of the light irradiation,and improve the signal to noise ratio.The experiment results are in goodagreement with the theoretical values.     

Design of the sample cell in near-field surface-enhanced Raman scattering by finite difference time domain method

The rational design of the sample cell may improve the sensitivity of surface-enhanced Raman scattering (SERS) detection in a high degree. Finite difference time domain (FDTD) simulations of the configuration of Ag film-Ag particles illuminated by plane wave and evanescent wave are performed to provide physical insight for design of the sample cell. Numerical solutions indicate that the sample cell can provide more "hot spots" and the massive field intensity enhancement occurs in these "hot spots". More information on the nanometer character of the sample can be got because of gradient-field Raman (GFR) of evanescent wave.     

Monitoring photo-thermal response and collagen remodeling of mouse skin with multiphoton microscopy

Two-photon excitation fluorescence （TPEF） and second-harmonic generation （SHG） are detected through multiphoton microscopy （MPM）. The major signals have the potential to monitor the process of tissue changes. TPEF and SHG are used to monitor the skin photo-thermal response to irradiation with intense pulsed light sources （A is in the range of 560-1200 nm） and trace the process of skin remodeling in vivo at different time intervals. TPEF intensity is nearly unchanged at different time intervals after irradiation, whereas SHG intensity changes considerably. The results reveal the photo-thermal effect of nonablative light sources and the process of collagen remodeling at the sub-micron level.     

低剂量辐射诱导hepG2细胞克隆存活和细胞周期适应性反应

以低剂量γ射线(0.05 Gy)预照射人肝癌细胞hep G2, 8 h后再用高剂量(3 Gy)照射, 测定了细胞的克隆存活率和细胞周期。 结果表明, 低剂量辐射预处理可诱导hep G2细胞产生克隆存活适应性反应, 并且有助于细胞通过G2/M期阻滞; 低剂量辐射诱导的克隆存活适应性反应与增强的通过细胞周期阻滞的能力之间有一定的相关性。 Human hepatoma cells hep G2 were irradiated with 3 Gy of γ ray 8 hours after primed with 0.05 Gy of γ ray, thereafter,cell survival and cell cycle were determined. The results indicated that both survival adaptive response and the enhanced ability to overcome G2/M arrest could be induced by pre irradiation with low dose of γ ray. It is suggested that there is a certain correlation between the survival adaptive response and the enhanced ability to overcome cell cycle arrest.