Supercritical fluid extraction of t-resveratrol and other phenolics from a spiked solid

Supercritical fluid extraction of spiked phenolics including gallic acid, (+)-catechin, (–)-epicatechin, caffeic acid, p-coumaric acid, myricetin, t-resveratrol, quercetin and salicylic acid from an inert support using pure CO₂ and methanol-carbon dioxide mixtures was studied. Extraction and collection variables including modifier percentage, extraction temperature, flow rate, extraction time, trap packing and rinse solvent were optimized. The study revealed that the use of methanol as modifier was mandatory. Only the less hydroxylated compounds such as p-coumaric acid, t-resveratrol and salicylic acid could be quantitatively recovered (mean recovery ≥ 95%) from spiked diatomaceous earth. Mean recoveries of more polar phenolic acids and flavonoids such as gallic acid, caffeic acid, catechins and quercetin were between 30% and 70%. Myricetin was not recovered at all. Received: 19 June 1997 / Revised: 20 October 1997 / Accepted: 26 October 1997     

Silicon-titanium xerogels: Synthesis and application to the determination of ascorbic acid and polyphenoles

Titanium and silicon-titanium xerogels have been obtained and it has been demonstrated that titanium(IV) incorporated into the xerogel matrix can participate in complexation reactions with ascorbic, gallic, ferulic, and caffeic acids and also rutin, quercetin, and dihydroquercetin present in aqueous solutions. In the case of dihydroquercetin 1 min of contact has been sufficient for achieving the equilibrium; the other compounds required 7–10 min. The stability constants of titanium(IV) complexes incorporated into xerogels reduced in the order dihydroquercetin > gallic acid > ascorbic acid > quercetin > caffeic acid > rutin. The procedures have been developed for solid-phase spectrophotometric determination of ascorbic, gallic, ferulic, caffeic acid as well as rutin, quercetin, and dihydroquercetin. The accuracy of the procedure has been examined by the standard addition method. The procedures for the determination of dihydroquercetin and ascorbic acid have been applied to the analysis of pharmaceutical preparations.     

Determination of phenolic acids by capillary zone electrophoresis and HPLC

Selected phenolic acids are determined by capillary zone electrophoresis and HPLC, each using UV detection. The optimised CZE background electrolyte contained 50 mM acetic acid, 95 mM 6-aminocaproic acid, 0.1% polyacrylamide, 1% polyvinylpyrrolidone, and 10% methanol. Twelve phenolic acids (gallic, p-hydroxybenzoic, 3,4-dihydroxybenzoic, vanillic, syringic, o-coumaric, p-coumaric, caffeic, sinapic, ferulic, salicylic and chlorogenic) were separated within 10 minutes. Chromatographic separation of these phenolic acids was carried out on an Eclipse XBD C8 column using a mobile phase gradient (acetonitrile / methanol / water / 0.1% phosphoric acid); all were separated within 25 minutes. Electrophoretic and chromatographic determinations of ferulic and chlorogenic acids were compared on barley, malt, and potato samples. The methods’ characteristics were: linearity (1–20 mg ml and 0.2–4 mg ml⁻¹), accuracy (recovery 94 ± 5% and 96 ± 4%), intra-assay repeatability (4.1% and 3.5%), and detection limit (0.2 and 0.02 mg ml⁻¹).      

Electrochemical behavior and antioxidant and prooxidant activity of natural phenolics

We have investigated the electrochemical oxidation of a number natural phenolics (salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, protocatechuic acid, o-coumaric acid, m-coumaric acid, p-coumaric acid, caffeic acid, quercetin and rutin) using cyclic voltammetry. The antioxidant properties of these compounds were also studied. A structural analysis of the tested phenolics suggests that multiple OH substitution and conjugation are important determinants of the free radical scavenging activity and electrochemical behavior. Compounds with low oxidation potentials (Epa lower than 0.45) showed antioxidant activity, whereas compounds with high Epa values (>0.45) act as prooxidants.     

Antioxidant and antimicrobial attributes and phenolics of different solvent extracts from leaves, flowers and bark of Gold Mohar [Delonix regia (Bojer ex Hook.) Raf

This paper describes the antioxidant and antimicrobial activities and phenolic components of different solvent (absolute methanol, absolute ethanol, absolute acetone, 80% methanol, 80% ethanol, 80% acetone and deionized water) extracts of leaves, flowers and bark of Gold Mohar [Delonix regia (Bojer ex Hook.) Raf.]. The extract yields from leaves, flowers and bark ranged from 10.19 to 36.24, 12.97 to 48.47 and 4.22 to 8.48 g/100 g dry weight (DW), respectively. Overall, 80% methanol extract produced from the leaves exhibited significantly (P < 0.05) higher antioxidant activity, with high phenolic contents (3.63 g GAE/100 g DW), total flavonoid contents (1.19 g CE/100 g DW), inhibition of peroxidation (85.54%), DPPH scavenging capacity (IC(50) value 8.89 μg/mL) and reducing power (1.87). Similarly, this 80% methanol leaves extract also showed superior antimicrobial activity. HPLC analysis of the 80% methanol extracts for individual phenolics revealed the presence of gallic, protocatechuic and salicylic acid in leaves; gallic, protocatechuic, salicylic, trans-cinnamic and chlorogenic acid in flowers, and gallic acid in bark as the main (amount > 1.50 mg/100 g DW) phenolic acids. Besides, small amounts ( < 1.50 mg/100 g DW) of some other phenolic acids such as sorbic, sinapic, p-coumaric, m-coumaric, ferulic, caffeic, 3-hydroxybenzoic, 4-hydroxycinnamic and 4-hydroxybenzoic acids were also detected. The extracts of the tested parts of Gold Mohar, especially, the leaves, might be valuable for functional food and therapeutic applications.     

Quantification of polyphenols with potential antioxidant properties in wines using reverse phase HPLC

A RP-HPLC method with photodiode array detection (DAD) was developed to separate, identify and quantify simultaneously the most representative phenolic compounds present in Madeira and Canary Islands wines. The optimized chromatographic method was carefully validated in terms of linearity, precision, accuracy and sensitivity. A high repeatability and a good stability of phenolics retention times (< 3%) were obtained, as well as relative peak area. Also high recoveries were achieved, over 80.3%. Polyphenols calibration curves showed a good linearity (r(2) >0.994) within test ranges. Detection limits ranged between 0.03 and 11.5 microg/mL for the different polyphenols. A good repeatability was obtained, with intra-day variations less than 7.9%. The described method was successfully applied to quantify several polyphenols in 26 samples of different kinds of wine (red, rosé and white wines) from Madeira and Canary Islands. Gallic acid was by far the most predominant acid. It represents more than 65% of all phenolics, followed by p-coumaric and caffeic acids. The major flavonoid found in Madeira wines was trans-resveratrol. In some wines, (-)-epicatechin was also found in highest amount. Canary wines were shown to be rich in gallic, caffeic and p-coumaric acids and quercetin.     

Solid-phase extraction procedure for determination of phenolic acids and some flavonols in honey

The solid-phase extraction procedure (SPE) for isolation and preconcentration of phenolic acids (gallic, p-HBA, p-coumaric, vanillic, caffeic and syringic acid) and some flavonols (rutin, quercetin and kaempferol) from honey samples prior to their determination by HPLC is reported. Different solid sorbents such as Bond Elut octadecyl C(18), Oasis HLB, Strata-X and Amberlite XAD-2 were tested for this purpose. The best results were obtain when aqueous solution of honey (100 mL) was adjusted to pH 2 and passed through the microcolumn containing 2.5 g of Oasis HB followed by washing the sorbent with 50 mL of acidified water (pH 2). The analytes were then eluted with methanol. The proposed method permits the quantification of the studied compounds with the limit of detections ranged from 25 ng kg(-1) to 0.75 microg kg(-1) for p-HBA and quercetin, respectively. The precision of the overall analytical procedure was estimated by measuring the within-day repeatability and the relative standard deviations of the parallel (n=3) results were in the range of 1.9-10.1%. The method was tested for real honey samples from different botanical origins.     

Phytochemical investigation and hepatoprotective activity of Cupressus sempervirens L. leaves growing in Egypt

Three phenolic compounds cosmosiin, caffeic acid, and p-coumaric acid were isolated for the first time from the leaves of Cupressus sempervirens L., together with cupressuflavone, amentoflavone, rutin, quercitrin, quercetin, myricitrin. The isolated compounds were identified using (1)H- and (13)C-NMR spectra. The hepatoprotective activity of the MeOH extract was carried out in liver homogenate of normal and CCl(4)-treated rats; a significant decrease in glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, cholesterol level, and triglycerides, while a significant increase in the total protein level, was observed after the oral administration of MeOH extract. The free radical scavenging activity against stable 2,2-diphenyl-2-picrylhydrazyl (DPPH*) was measured for MeOH extract and some of the isolated phenolic compounds in comparison with alpha-tocopherol and butylated hydroxy toluene as standard antioxidants using ESR technique, showed high antioxidant activity for quercetin, rutin, caffeic acid, and p-coumaric acid.     

Comparison of the contents of various antioxidants of sea buckthorn berries using CE

The increased interest in sea buckthorn (Hippophae rhamnoides L.) made it possible to investigate the antioxidant content in it. To address this issue, the presence of following antioxidant compounds were analyzed: trans-resveratrol, catechin, myricetin, quercetin, p-coumaric acid, caffeic acid, L-ascorbic acid (AA), and gallic acid (linear range of 50-150 micromol/L) in six different varieties of sea buckthorn berries extracts (sea buckthorn varieties: "Trofimovskaja (TR)," "Podarok Sadu (PS)," and "Avgustinka (AV),") received from two local Estonian companies. Trans-Resveratrol, catechin, AA, myricetin, and quercetin were found in extracts of sea buckthorn. Moreover, AA, myricetin, and quercetin contents were quantified. The biggest average AA content was found in TR (740 mg/100 g of dried berries, respectively). Furthermore, the same varieties gave the biggest quercetin content 116 mg/100 g of dried berries, respectively. For analysis, CZE was used and the results were partly validated by HPLC. Statistically no big differences in levels of antioxidants were consistently found in different varieties of sea buckthorn extracts investigated in this work.     

Determination of anti-oxidant capacity and content of phenols,phenolic acids,and flavonols in Indian and European gooseberry

Phenolic acids and derivatives of quercetin in Indian (amla) and European gooseberry were determined by high-performance liquid chromatography with diode array detector. The calibration curves were constructed using phenolic compounds standards (the coefficient of determination (R ²) was 0.9990–0.9997 for phenolic acids and 0.9989–0.9994 for flavonols, respectively). The lowest detection limit was 0.28 mg L⁻¹ and 0.35 mg L⁻¹ for hyperoside and gallic acid, respectively, whereas the highest was 1.80 mg L⁻¹ and 7.98 mg L⁻¹ for quercetin and chlorogenic acid, respectively. The quantification limits calculated were 0.85–24.04 mg L⁻¹ for hyperoside and chlorogenic acid, respectively. The predominant phenolic acid in amla and gooseberry is gallic acid: (5.37 ± 0.04) mg per 100 g of dry mass (d.m.) and (3.21 ± 0.03) mg per 100 g of d.m., respectively. The next one was caffeic acid, 0.65–1.22 mg per 100 g of d.m., followed by p-coumaric acid, 0.84–1.17 mg per 100 g of d.m. Out of the flavonols, rutin is predominant: (3.11 ± 0.13) mg per 100 g of d.m. and (2.12 ± 0.03) mg per 100 g of d.m., respectively. Anti-oxidant activity was also determined.     

GC-MS quantitation of benzoic and aralkyl carboxylic acids as their trimethylsilyl derivatives: In model solution I

Summary This paper describes the fragmentation patterns and the GC-MS quantitation possibilities of the trimethylsilyl derivatives of thirty-one aromatic carboxylic acids, using ion trap detection (ITD). Sixteen aralkyl carboxylic acids, including those containing a saturated aliphatic side chain {phenylacetic, 2-phenylbutyric, phenylglycolic (mandelic acid), β-phenyllactic, 3-hydroxyphenylacetic, β-phenylpyruvic and 3-(4-hydroxyphenyl)-propionic acids} and those with an unsaturated aliphatic side chain {cinnamic, 2-hydroxycinnamic (o-coumaric), 4-methoxycinnamic, 3-hydroxycinnamic (m-coumaric), 4-hydroxycinnamic (p-coumaric), 4-hydroxy-3-methoxycinnamic (ferulic acid), 3,4-dihydroxycinnamic (caffeic), and 4-dihydroxy-3,5-dimethoxycinnamic (sinapic) acids}, as well as, the fifteen hydroxy(methoxy) benzoic acids {benzoic, 2-hydroxybenzoic (salicylic), 3-hydroxybenzoic, 4-hydroxybenzoic, 3,5-dimethoxybenzoic, 3,4-dimethoxybenzoic (veratric), 2,6-dihydroxybenzoic (γ-resorcylic), 3-methoxy-4-hydroxybenzoic (vanillic), 2,5-dihydroxybenzoic (gentisic), 2,4-dihydroxybenzoic (β-resorcylic), 3,4-dihydroxybenzoic (protocatechuic), 3,5-dihydroxybenzoic (α-resorcylic), 2,4,5-trimethoxybenzoic (asaronic), 3,5-dimethoxy-4-hydroxybenzoic (syringic) and 3,4,5-trihydroxybenzoic (gallic) acids}, provided distinct fragmentation characteristics that were very useful for their identification and simultaneously quantitation. Based on 1–20 ng amounts of acids, very informative ions of high mass with considerable intensities ([M+TMS]⁺, [M+1]⁺), , ([M−CH₃]⁺) were obtained. In the case of the cinnamic acid derivatives, several odd electron fragments are formed by the loss of CO, HCHO and/or Si(CH₃)₄ molecules. In the case of benzoic acids the molecular ion proved to be abundant in three, the [M−CH₃]⁺ ion in nine cases out of fifteen. The special MacLafferty rearrangement product ([C₆H₅Si(CH₃)₂]⁺) was obtained in different yields. In addition to the TIC values, at least three, and in most cases four, selective fragment ions could be utilized for quantitation. The reproducibility of the data in the concentration range of 1–20 ng acids proved to be between 1.2 and 13.0% (R.S.D.). Presented at: Balaton Symposium on High-Performance Separation Methods, Siófok, Hungary, September 3–5, 1997     

A silver nanoparticle-based method for determination of antioxidant capacity of rapeseed and its products

A novel silver nanoparticle-based (AgNP) method and two modified procedures, ferric reducing antioxidant power (FRAP) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH), were used for determination of antioxidant capacities of the ethanolic, methanolic, methanolic-aqueous (1?:?1 v/v) and aqueous extracts of rapeseed and its products. The AgNP method based on the electron-transfer reaction between silver ions and antioxidants in an optimized ammonium buffer medium (pH = 8.4) and determination of silver nanoparticle formation has been elaborated. The novel AgNP method was validated using sinapic acid, gallic acid, caffeic acid, ascorbic acid and quercetin as standard antioxidant solutions in concentration ranges of 0.03-0.21 μmol mL(-1), 0.02-0.20 μmol mL(-1), 0.01-0.18 μmol mL(-1), 0.03-0.30 μmol mL(-1) and 0.001-0.009 μmol mL(-1). The calculated detection (DL = 0.01, 0.02, 0.009, 0.02 and 0.0004 μmol mL(-1) for sinapic, gallic, caffeic, ascorbic acids and quercetin, respectively) and quantification limits (QL = 0.04, 0.06, 0.03, 0.08 and 0.001 μmol mL(-1) for sinapic, gallic, caffeic, ascorbic acids and quercetin, respectively) confirm linearity concentration ranges for determination of antioxidant capacity by AgNP assay. The average antioxidant capacities of the studied rapeseed samples ranged between 14.7 and 126.2 μmol sinapic acid per gram for the proposed AgNP method, 7.4-112.7 μmol sinapic acid per gram for the FRAP method and 39.1-339.8 μmol sinapic acid per gram for DPPH assay. The methanol-water mixture (1:1 v/v) was the most efficient solvent for extraction of antioxidants from the studied rapeseed samples. There are significant, positive correlations between the novel AgNP and the modified FRAP, DPPH and FC methods for all extracts of the studied rapeseed samples (r = 0.7564-0.8516, p < 0.001). Satisfactory values of precision (RSD = 1.2-4.4%) and accuracy (recovery = 95.6-104.6%, except methanolic extracts) demonstrate the benefit of the proposed AgNP method for analysis of the antioxidant capacity of rapeseed samples. Results of the principal component analysis (PCA) indicate that there are differences between the total amounts of antioxidants in rapeseed samples extracted by different solvents.     

Optimization of microwave-assisted extraction of polyphenols from apple pomace using response surface methodology and HPLC analysis

A simple and efficient microwave-assisted extraction of polyphenols from industrial apple pomace was developed and optimized by the maximization of the yield using response surface methodology. A Box-Behnken design was used to monitor the effect of microwave power, extraction time, ethanol concentration and ratio of solvent to raw material (g/mL) on the polyphenols yield. The results showed that the optimal conditions were as follows: microwave power 650.4?W, extraction time 53.7?s, ethanol concentration 62.1% and ratio of solvent to raw material 22.9:1. Validation tests indicated that the actual yield of polyphenols was 62.68±0.35?mg gallic acid equivalents per 100?g dry apple pomace with RSD=0.86% (n=5) under the optimal conditions, which was in good agreement with the predicted yield and higher than those of reflux and ultrasonic-assisted extraction methods. HPLC analysis indicated that the major polyphenols of apple pomace consisted of chlorogenic acid, caffeic acid, syrigin, procyanidin B2, (-)-epicatechin, cinnamic acid, coumaric acid, phlorizin and quercetin, of which procyanidin B2 had the highest content of 219.4?mg/kg.     

Optimisation of supercritical fluid extraction of polycyclic aromatic hydrocarbons and their nitrated derivatives adsorbed on highly sorptive diesel particulate matter

Supercritical fluid extraction (SFE) was performed to extract complex mixtures of polycyclic aromatic hydrocarbons (PAHs), nitrated derivatives (nitroPAHs) and heavy n-alkanes from spiked soot particulates that resulted from the incomplete combustion of diesel oils. This polluted material, resulting from combustion in a light diesel engine and collected at high temperature inside the particulate filter placed just after the engine, was particularly resistant to conventional extraction techniques, such as soxhlet extraction, and had an extraction behaviour that differed markedly from certified reference materials (SRM 1650). A factorial experimental design was performed, simultaneously modelling the influence of four SFE experimental factors on the recovery yields, i.e.: the temperature and the pressure of the supercritical fluid, the nature and the percentage of the organic modifier added to CO₂ (chloroform, tetrahydrofuran, methylene chloride), as a means to reach the optimal extraction yields for all the studied target pollutants. The results of modelling showed that the supercritical fluid pressure had to be kept at its maximum level (30 MPa) and the temperature had to be kept relatively low (75 °C). Under these operating conditions, adding 15% of methylene chloride to the CO₂ permitted quantitative extraction of not only light PAHs and their nitrated derivatives, but also heavy n-alkanes from the spiked soots. However, heavy polyaromatics were not quantitatively extracted from the refractory carbonaceous solid surface. As such, original organic modifiers were tested, including pyridine, which, as a strong electron donor cosolvent (15% into CO₂), was the most successful. The addition of diethylamine to pyridine, which enhanced the electron donor character of the cosolvent, even increased the extraction yields of the heaviest PAHs, leading to a quantitative extraction of all PAHs (more than 79%) from the diesel particulate matter, with detection limits ranging from 0.5 to 7.8 ng for 100 mg of spiked material. Concerning the nitrated PAHs, a small addition of acetic acid into pyridine, as cosolvents, gave the best results, leading to fair extraction yields (approximately 60%), with detection limits ranging from 18 to 420 ng.     

Mesoporous silica SBA-15, a new adsorbent for bioactive polyphenols from red wine

The aim of this paper is to evaluate the ability of the mesoporous silica SBA-15 to adsorb polyphenols from red wine. The mesoporous molecular sieve silica SBA-15 was hydrothermally synthesized in acidic media and characterized by SAXRD, BET, EDX and SEM. The adsorption behavior of mesoporous silica SBA-15 was investigated at 5 °C for 24 h using an adsorbent dose of 8 g SBA-15 L⁻¹ red wine. The total polyphenols content expressed as mg of gallic acid equivalents (GAE L⁻¹) was estimated from the standard curve of gallic acid (absorbance at 280 nm). HPLC chromatograms of methanolic extract from mesoporous SBA-15 at 256, 280, 324, and 365 nm exhibits the strong retention of quercetin and cis-resveratrol and a reasonable retention of trans-resveratrol, catechin, epicatechin, rutin, and phenolic acids (meta- and para-hydroxybenzoic, vanillic, caffeic, syringic, salicylic and para-coumaric acids).     

Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay

We report on the application of a simple and versatile antioxidant capacity assay for dietary polyphenols, vitamin C and vitamin E utilizing the copper(II)-neocuproine (Cu(II)-Nc) reagent as the chromogenic oxidant, which we term the CUPRAC (cupric reducing antioxidant capacity) method. It involves mixing the antioxidant solution (directly or after acid hydrolysis) with solutions of CuCl₂, neocuproine, and ammonium acetate at pH 7, and measuring the absorbance at 450 nm after 30 min. Slowly reacting antioxidants required an incubation at 50 °C for 20 min for color development. The flavonoid glycosides were hydrolyzed to their corresponding aglycones by refluxing in 1.2 M HCl-containing 50% MeOH for fully exhibiting their antioxidant potencies. Certain compounds also needed incubation after acid hydrolysis for color development. The CUPRAC absorbances of mixture constituents were additive, indicating lack of chemical deviations from Beer’s law. The CUPRAC antioxidant capacities of a wide range of polyphenolics are reported in this work and compared to those found by ABTS/persulfate and Folin assays. The trolox-equivalent capacities of the antioxidants were linearly correlated (r = 0.8) to those found by ABTS but not to those of Folin. The highest antioxidant capacities in the CUPRAC method were observed for epicatechin gallate, epigallocatechin gallate, quercetin, fisetin, epigallocatechin, catechin, caffeic acid, epicatechin, gallic acid, rutin, and chlorogenic acid in this order, in accordance with theoretical expectations. The experiences of other CUPRAC users also are summarized. Correspondence: Reşat Apak, Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar, TR-34320 Istanbul, Turkey     

Determination of phenolic compounds in medicinal herbs by reversed-phase HPLC

An HPLC procedure is proposed for the determination of 12 phenolic compounds in plant tissues by reversed-phase HPLC with gradient elution and UV detection. The influence of pH, composition of the mobile phase, concentration of the organic modifier, and temperature on the separation of gallic, protocatechuic, trans-ferulic, trans-caffeic acid, rutin, quercetin, dihydroquercetin, and (_)-epicatechin for 30 min is studied. The lower limit of quantification of phenolic compounds is 1–2.5 μg/L. The procedure was applied to the determination of phenolic compounds in aqueous extracts of Hypericum perforatum; its sample was found to contain protocatechuic acid, (_)-epicatechin, and also rutin.     

Simultaneous determination of phenolic acids and flavonoids in rice using solid-phase extraction and RP-HPLC with photodiode array detection

An analytical method based on an optimized solid-phase extraction procedure and followed by high-performance liquid chromatography (HPLC) separation with diode array detection was developed and validated for the simultaneous determination of phenolic acids (gallic, protocatechuic, 4-hydroxy-benzoic, vanillic, caffeic, syringic, p-coumaric, ferulic, sinapic, and cinnamic acids), flavanols (catechin and epicatechin), flavonols (myricetin, quercetin, kaempferol, quercetin-3-O-glucoside, hyperoside, and rutin), flavones (luteolin and apigenin) and flavanones (naringenin and hesperidin) in rice flour (Oryza sativa L.). Chromatographic separation was carried out on a PerfectSil Target ODS-3 (250 mm × 4.6 mm, 3 μm) column at temperature 25°C using a mobile phase, consisting of 0.5% (v/v) acetic acid in water, methanol, and acetonitrile at a flow rate 1 mL min(-1) , under gradient elution conditions. Application of optimum extraction conditions, elaborated on both Lichrolut C(18) and Oasis HLB cartridges, have led to extraction of phenolic acids and flavonoids from rice flour with mean recoveries 84.3-113.0%. The developed method was validated in terms of linearity, accuracy, precision, stability, and sensitivity. Repeatability (n = 5) and inter-day precision (n = 4) revealed relative standard deviation (RSD) <13%. The optimized method was successfully applied to the analysis of phenolic acids and flavonoids in pigmented (red and black rice) and non-pigmented rice (brown rice) samples.     

Microwave-Assisted Efficient Extraction and Stability of Juglone in Different Solvents from Juglans regia: Quantification of Six Phenolic Constituents by Validated RP-HPLC and Evaluation of Antimicrobial Activity

Abstract

In the present study, microwave-assisted extraction was compared with conventional approaches for the efficient extraction of juglone and other phenolics from Juglans regia bark. The effect of different solvents was also studied and ethyl acetate was found to be a better solvent in terms of juglone yield and stability. Further, a simple and fast RP-HPLC method was developed and validated for the determination of juglone and other bioactive phenolics like gallic acid, caffeic acid, quercetin, myricetin, and quercitrin in these extracts. In addition, the extracts were tested for antimicrobial activity against 16 microorganisms where all the extracts showed broad spectrum activity.