Separation of zirconium by thin-layer chromatography

The thin-layer Chromatographie separation of a number of metal ions [Sc, Y, Zr, La, Sm, Th, U(VI), etc.] with solvent mixtures of mesityl oxide, ethanol and 5M nitric acid on silica gel-cellulose (5:1) thin-layer plates is reported. Zirconium remains stationary whilst the other metal ions move with the solvent, thus allowing a selective separation of zirconium from about 20 metal ions in ratios ranging from 100:1 to 1:100. Mixtures of various metal ions can also be separated.     

Separation of carbohydrates by thin-layer chromatography

Summary The separation of 13 sugars by means of 8 solvents and one- and two-dimensional chromatography on cellulose and starch layers is described. Good results were obtained on cellulose layers.

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Zusammenfassung Die Trennung 13 verschiedener Zucker mit 8 Lösungsmitteln im Wege der ein- und zweidimensionalen Chromatographie auf Zelluloseund Stärkedünnschichten wurde beschrieben. Zelluloseschichten ergeben gute Resultate.

     

Separation of fat-soluble vitamins by thin-layer chromatography

Summary This paper is dealing with the evaluation of the method for qualitative analyses of fat soluble vitamins by thin-layer chromatography on impregnated starch, cellulose and talc thin layers. Impregnation was carried out with paraffin oil. Complete analyses could be obtained on two plates with two different solvents. Vitamins K₃, K₄ and K₅ were separated with solvent I (water-dioxane-acetone-formaldehyde (85∶20∶15∶25)) whereas the other remain on the start position. Vitamins A-acetate, A-palmitate, K₁, DL-alpha-tocopherol, DL-alpha-tocopherol-acetate, D₂, D₃ were separated with solvent II (acetone-concentrated acetic acid (30∶20)) while K₃, K₄ and K₅ migrate with front. Quick and simple procedures for the preparation of the plates with an impregnated thin layer is given.     

Separation of nebramycin components by thin-layer chromatography

A thin-layer chromatographic method has been developed for the detection of major nebramycin components, for the separation of tobramycin from other components and for studying the hydrolysis of carbamoyl derivatives and procedures for isolation and purification. A sensitive method has also been established for the detection of kanamycin B in tobramycin and for the assay of apramycin in kanamycin B.