STUDIES ON TRANSPLANTATION OF THE LAMININ RECEPTOR AND ITS ROLES IN EXPERIMENTAL METASTASIS OF MURINE LEWIS LUNG CARCINOMA CELLS

The isolated laminin receptor (LN-R) labeled by ~(125)I was reconstituted into liposomes.~(125)I-LN-R-liposomes and free ~(125)I-LN-R were separated by Sepharose 4B column chromatogra-phy. The LN-R-liposomes showed affinity for laminin (LN) and were capable of binding toimmobilized LN substrate. In order to make transplantation of LN-R, LN-R-liposomes werefused with cultured murine Lewis lung carcinoma cells with the help of polyethylene glycol(PEG) induction. The radiation with the fused cells was not removed by salt sclution. Thebinding of the fused cells enriched in foreign LN-R to LN substrate increased by 87.5%.Furthermore, the murine Lewis lung carcinoma cells with and without transplanted LN-Rwere injected into C_(57)BL/6J mice through tail veins (5×10~5 cells/each mouse)respectively.The mice in the test group died earlier than those in the control group. The total weight oflung tumor in the test group remarkably increased in comparison with those in the controlgroup. The results taken together     

PROMOTIONAL EFFECTS OF ULTRAVIOLET RADIATION ON HUMAN BASAL AND SQUAMOUS CELL CARCINOMA

Abstract— Recent data on the incidence of basal and squamous cell skin cancer among Caucasians in eight regions of the United States have been analyzed. Principal conclusions are that (1) ultraviolet radiation is an effective promoter of non-melanoma skin tumors; (2) the probability of a skin tumor becoming observable in an individual of some given age is well described by the log-normal distribution: (3) the fluence-response relation for both basal and squamous cell tumors is linear with a positive intercept, although the parameter values are clearly different for the two types; (4) a 1% ozone layer depletion would lead to an eventual 1.7% increase in basal cell carcinoma, and a 2.3% increase in squamous cell carcinoma.     

单克隆抗体免疫吸附剂的制备及对乙型肝炎表面抗原的吸附

通过环氧氯丙烷活化（１，４） ２ 氨基 ２ 脱氧 β Ｄ 葡萄糖（壳聚糖）制备免疫吸附剂，详细讨论了环氧氯丙烷活化过程的影响因素，确定了活化的最佳条件．活化（１，４） ２ 氨基 ２ 脱氧β Ｄ 葡萄糖树脂对单克隆抗体的偶联率为１７６％，对含乙型肝炎表面抗原阳性血清的吸附实验表明，制备的免疫吸附剂能使阳性转为阴性，吸附率为４４％．