Label-Free Microscopic Imaging Based on the Random Matrix Theory in Wavefront Shaping

Wavefront shaping technology has mainly been applied to microscopic fluorescence imaging through turbid media,with the advantages of high resolution and imaging depth beyond the ballistic regime. However, fluorescence needs to be introduced extrinsically and the field of view is limited by memory effects. Here we propose a new method for microscopic imaging light transmission through turbid media, which has the advantages of label-free and discretional field of view size, based on transmission-matrix-based wavefront shaping and the random matrix theory. We also verify that a target of absorber behind the strong scattering media can be imaged with high resolution in the experiment. Our method opens a new avenue for the research and application of wavefront shaping.     

Active spectral filtering through turbid media

We demonstrate controlled wavelength-dependent light focusing through turbid media using wavefront shaping. Due to the dispersion caused by multiple light scattering, light propagation through turbid media can be independently controlled between different wavelengths. Foci with various wavelengths can be generated by applying different optimized wavefronts to a highly scattering layer. Given the linearity of the transmission matrix, multiple foci with different wavelengths can also be simultaneously constructed.     

Photoacoustic-guided convergence of light through optically diffusive media

We demonstrate that laser beams can be converged toward a light-absorbing target through optically diffusive media by using photoacoustic-guided interferometric focusing. The convergence of light is achieved by shaping the wavefront of the incident light with a deformable mirror to maximize the photoacoustic signal, which is proportional to the scattered light intensity at the light absorber.     

Wavefront Shaping for Fast Focusing Light through Scattering Media Based on Parallel Wavefront Optimization and Superpixel Method

When light travels in biological tissues,it undergoes multiple scattering and forms speckles,which seriousl.y restricts the penetration depth of optical imaging in biological tissues.With wavefront shaping method,by modulating the wavefront of incident light to compensate for the wavefront aberration,light focusing and scanning imaging through scattering media can be achieved.However,wavefront shaping must be accomplished within the speckle decorrelation time.Considering the short speckle decorr...     

四元裂解位相调制实现相干光通过散射介质聚焦

光在不均匀介质中传播会受到散射的干扰,在这些散射材料中,例如粉末、生物组织、亚波长颗粒对入射光多次散射使得出射光无法聚焦,从而在接收平面形成散斑.本文提出四元裂解位相调制方法对入射相干光场进行调制,使其通过散射介质聚焦.此方法利用入射光场全场调制,充分考虑光场单元之间的干涉作用,从整个空间光调制器的调制面开始,逐层进行四元裂解及位相优化.运用此方法在实验中实现了相干光的前向散射和后向散射有效聚焦,这为生物医学领域中通过散射介质成像提供了新的思路和方法.     

Measurements of diffuse backscattering Mueller matrices of turbid media

We report on the development of a method that records spatially dependent intensity patterns of polarized light that arise from illuminating a turbid media with a polarized laser beam and being diffusely backscattered. Our technique employs polarized light from a He-Ne laser () which is focused onto the surface of the scattering medium. A surface area of approximately 2×2 cm centered on the light input point is imaged through polarization analysis optics onto a CCD camera. The Mueller matrix is reconstructed by 49 intensity measurements with various orientations of polarizer and analyzer. The measured Mueller matrix of polystyrene spheres was compared with the theory result of incoherent scattering of light by spheres. The experimental and theory results are in excellent agreement. It appears that the azimuthal patterns of the Mueller matrix are determined by the symmetry of the turbid media.     

Phase control algorithms for focusing light through turbid media

Light propagation in materials with microscopic inhomogeneities is affected by scattering. In scattering materials, such as powders, disordered metamaterials or biological tissue, multiple scattering on sub-wavelength particles makes light diffuse. Recently, we showed that it is possible to construct a wavefront that focuses through a solid, strongly scattering object. The focusing wavefront uniquely matches a certain configuration of the particles in the medium. To focus light through a turbid liquid or living tissue, it is necessary to dynamically adjust the wavefront as the particles in the medium move. Here we present three algorithms for constructing a wavefront that focuses through a scattering medium. We analyze the dynamic behavior of these algorithms and compare their sensitivity to measurement noise. The algorithms are compared both experimentally and using numerical simulations. The results are in good agreement with an intuitive model, which may be used to develop dynamic diffusion compensators with applications in, for example, light delivery in human tissue.     

Controlling single-photon Fock-state propagation through opaque scattering media

The control of light scattering is essential in many quantum optical experiments. Wavefront shaping is a technique used for ultimate control over wave propagation through multiple-scattering media by adaptive manipulation of incident waves. We control the propagation of single-photon Fock states through opaque scattering media by spatial phase modulation of the incident wavefront. We enhance the probability that a single photon arrives in a target output mode with a factor 30. Our proof-of-principle experiment shows that the propagation of quantum light through multiple-scattering media can be controlled, with prospective applications in quantum communication and quantum cryptography.     

Parallel wavefront optimization method for focusing light through random scattering media

A parallel wavefront optimization method is demonstrated experimentally to focus light through random scattering media. The simultaneous modulation of multiple phase elements, each at a unique frequency, enables a parallel determination of the optimal wavefront. Compared to a pixel-by-pixel measurement, the reported parallel method uses the target signal in a highly efficient way. With 441 phase elements, a high-quality focus was formed through a glass diffuser with a peak-to-background ratio of ～270. The accuracy and repeatability of the system were tested through experiments.     

Transmission of polarized light through turbid media

The depolarization of light in multiple-scattering media with large (larger than the light wavelength) inhomogeneities is considered. The polarization state of the scattered light is described in the principal-mode approximation. Using the Fokker-Planck model, the polarization and intensity distribution of light are calculated in the vicinity of an inhomogeneity in the shape of an absorbing half-plane. The results of the calculations agree with the experimental data on transmission of light through turbid media.     

Effects of feedback from collimating, focusing, and spot-array generating outcoupler gratings in surface-emitting semiconductor lasers

We experimentally demonstrate that a grating outcoupler used for complex beam shaping (spot-array generation) can produce unintentional optical feedback that severely disturbs the integrated in-plane laser that illuminates the coupler. Simulations show that these outcouplers, in contrast to conventional collimating or focusing outcouplers, tend to produce high levels of feedback in spite of the detuning used to suppress feedback. Further, this feedback is focused to high intensity in the laser gain medium. This focused light acts as a seed for the nonlinear self-focusing that causes wave-front distortion and filamentation, which degrades the beam quality.     

混浊大气介质调制传递函数的一般特征

基于混浊介质的调制传递函数(MTF)和在各向同性漫射光源照射下平行平面混浊介质出射光强度分布之间的等效原理,利用辐射传输算法DISORT数值计算了几种典型的均匀大气混浊介质的MTF,获得了整个空间频域内MTF的一般特征.结果显示,大气介质的MTF不但依赖于介质的光学厚度(散射和吸收),也依赖于介质的散射相函数.给出了大...     

Performance analysis of field-of-view shifted Shack–Hartmann wavefront sensor based on splitter

We report on a field-of-view shifted Shack–Hartmann wavefront sensor based on a splitter designed to measure the wavefront information of object light with strong background. The analysis results indicate that increasing the ratio of the signal beam intensity to the incoming beam intensity and reducing the sampling frequency of CCD₂ (detecting the pure background light) can properly enhance the SNR (signal-to-noise ratio) of the object light. A special noise removing algorithm was presented to enhance the centroid measuring accuracy, and it was proved to be effective against the residual noises caused by the differences between the two beam paths. The experimental results indicate that the field-of-view shifted Shack–Hartmann wavefront sensor based on a splitter can exactly measure the wavefront information from the object with strong background. PACS 42.68.Bz; 47.50.Ef; 42.30.Wb     

Near-diffraction-limited dark hollow beam generated by using a hybrid control way

We propose and demonstrate the generation of dark hollow beam with plane wavefront in the near field based on a hybrid control way by using dual phase only liquid crystal spatial light modulators (LC-SLMs). One phase only LC-SLM redistributes the intensity to the target distribution based on refractive beam shaping system and the other phase only LC-SLM adaptively compensates the wavefront of the output beam by using stochastic parallel gradient descent (SPGD) algorithm. Compared with conventional refractive beam shaping system, the hybrid control way can not only improve the far-field energy concentration of the output beam, but also can correct the phase distortion of the system. Influences of phase distortion on laser beam shaping are analyzed in detail. The theoretical and experimental results show that the near-diffraction-limited dark hollow beam can be successfully generated by using this hybrid control way. The power in the main lobe of the airy disk pattern area in the far field is 2.3 times larger than that without being adaptively compensated.     

Polarization characteristics of backscattering of turbid media based on Mueller matrix

Mueller matrix is one approach to characterizing optical polarization of the turbid media. We have simulated the two-dimensional images of Mueller matrix based on single-scattering approximation model and implemented experiments to verify the simulations. By comparing the experimental results to the theoretical simulations, we have obtained some conclusions. When the particle size is smaller than the wavelength, the linearly polarized light propagating through the turbid media of Rayleigh scatterers has better polarization-maintaining ability. Whereas when the particle size is larger than the wavelength, the circularly polarized light propagating through the turbid media of Mie scatterers has better polarization-maintaining ability. Moreover, the radial dependence of the element patterns becomes weak as the transport mean free path decreases. This study can help us understand to the fundamental principle of optical polarization.     

Longitudinal intensity distribution near the focus produced by light through scattering media

Speckle pattern is formed when coherent light passes through scattering media. It has been demonstrated that after appropriately optimizing the phase of the incident light, a bright focal spot in the target point can be obtained for the case that the light passes through the scattering medium. However, until now the focused intensity distribution near the focus seems unclear. In this paper we experimentally investigate some factors influencing the longitudinal intensity distribution near the focus in details. It is shown that the desired longitudinal focused intensity distribution can be obtained by optimizing the incident light. The results may have potential applications in particle manipulation and laser processing, etc.     

Three-dimensional spatial multi-point uniform light focusing through scattering media based on feedback wavefront shaping

We use feedback wavefront shaping technology to realize the multi-point uniform light focusing in three-dimensional(3 D) space through scattering media only by loading the optimal mask once.General 3 D spatial focusing needs to load the optimal mask multiple times to realize the spatial movement of the focal point and the uniformity of multi-point focusing cannot be guaranteed.First,we investigate the effects of speckle axial correlation and different axial distances on 3 D spatial multi-point uniform focusing and propose possible solutions.Then we use our developed non-dominated sorting genetic algorithm suitable for 3 D spatial focusing(S-NSGA) to verify the experiment of multi-point focusing in 3 D space.This research is expected to have potential applications in the fields of optical manipulation and optogenetics.     

Turbid media optical properties derived from the characteristics of propagating laser radiation beams

The possibility is studied to develop a straightforward analytical approach to the determination of the optical properties of liquid turbid media having forward-peaked scattering indicatrices. The approach is based on investigating the in-depth behavior of the radius and the axial intensity of a laser radiation beam propagating through the turbid medium. Based on the small-angle approximation, the detected forward-propagating light power spatial distribution, at relatively small or large optical depths along the beam axis, is obtained asymptotically in analytical form allowing one to derive relatively simple expressions of the extinction, reduced-scattering and absorption coefficients and the anisotropy factor of the medium through the characteristics of the propagating light beam. Preliminary experiments have also been performed, using Intralipid dilutions of different relatively low concentrations and measuring the cross-sectional radial distribution of the detected light power at different depths along the beam axis. The corresponding on-axis detected light power profiles have been measured independently as well. The experimental results are consistent with the analytical expressions obtained that allow one to estimate the optical coefficients and the anisotropy factor of the investigated media on the basis of the measured beam characteristics. The values obtained are near those predicted by other researchers.     

三能级原子系统中单光子频率失谐对光减速的影响

在Λ型三能级Rb原子介质中，观察到了由电磁感应透明（EIT）效应导致的光减速现象并测 量了光延迟对单光子频率失谐量的依赖关系. 结果表明，由于多普勒展宽效应的存在，在单 光子频率失谐±600MHz的范围内，光减速效应较为显著. 在考虑多谱勒频移的情况下，数值 计算了光延迟随单光子频率失谐量的变化曲线，实验结果与理论曲线很好地符合. 这一研 究结果为利用单光子频率失谐控制光的群速度提供了理论与实验参考. 关键词： 光减速 电磁感应透明 多普勒展宽     

基于光调制技术的混浊介质空间散射光信号检测方法研究

为提高被测混浊介质样品漫反射、漫透射和准直透射光信号的测量信噪比,首次将光调制技术运用于空间多点特征光信号的检测中。测量系统以调制盘转动频率对样品入射光强进行频率调制,然后对采集的多点光强数据通过离散傅里叶变换进行解调,提取调制频率点的谐波分量作为测量值。用该方法对浓度为8%,直径为0.7μm的聚苯乙烯小球悬浮液进行了实验测试,结果表明该方法能有效提高被测样品特征光信号的测量信噪比,为强噪声背景下混浊介质光学参数研究提供了新的途径和方法。