共查询到14条相似文献,搜索用时 15 毫秒
1.
Jeong Gwi-Taek Park Don-Hee Ryu Hwa-Won Lee Woo-Tai Park Kyungmoon Kang Choon-Hyoung Hwang Baik Woo Je-Chang 《Applied biochemistry and biotechnology》2002,98(1-9):1129-1139
Panax ginseng hairy roots were transformed by Agrobacterium rhizogenes KTCT 2744. They showed an active branching pattern and fast growth in hormone-free medium, and good growth at 23°C, pH 5.8,
1/2 MS medium, and 3% sucrose. Sucrose provided the highest growth among seven carbon sources tested. Six complex media were
also tested. In the combined sugar study, hairy roots grew better on sucrose without glucose or fructose than with glucose
or fructose. In the 1/2 MS basal medium, 30 mM in nitrogen and 0.62 mM phosphate salt concentration was the optimum. The growth ratio was maximal at an inoculum size of 0.4% (w/v). Crude saponin
and polysaccharide levels were also measured. 相似文献
2.
Gwi-Taek Jeong Don-Hee Park Baik Hwang Je-Chang Woo 《Applied biochemistry and biotechnology》2003,107(1-3):493-503
This study investigated the effects of flask-to-liquid volume ratio on the growth of Panax ginseng hairy root, transformed by Agrobacterium rhizogenes, in flask cultures and compared the characteristics of various bioreactors for scale-up. The flask-to-liquid volume ratio
was optimum at 1.5 mL of air/mL of medium in flask cultures, and hairy root growth was not affected above the optimum ratio.
In 500-mL flask culture, hairy root showed two growth phases. After the first exponential growth, specific growth rate decreased.
The growth characteristics of P. ginseng hairy root in various bioreactors were investigated. Hairy root growth was about 55-fold of inoculum after 39 d in a 5-L
bioreactor and about 38-fold of inoculum after 40 d in a 19-L bioreactor. Carbon yield was higher in a 19-L bioreactor than
in others, but it did not show any linear relationship to the growth rate of hairy roots in bioreactors. 相似文献
3.
Gwi-Taek Jeong Don-Hee Park Hwa-Won Ryu Baik Hwang Je-Chang Woo 《Applied biochemistry and biotechnology》2004,116(1-3):1193-1203
Plants have a potential to produce a large number of important metabolites such as pharmaceuticals, food additives, pigments,
flavors, fragrances, and fine chemicals. Large-scale plant cell and tissue cultures for producing useful products has been
considered an attractive alternative to whole plant extraction for obtaining valuable chemicals. In plant cell and tissue
cultures, cell growth and metabolite production are influenced by nutritional and environmental conditions as well as physical
properties of the culture system. To obtain a high growth rate of plant cell and tissue cultures, the culture tem. To obtain
a high growth rate of plant cell and tissue cultures, the culture conditions should be maintained at an optimum level. We
studied the relationship between inoculum conditions and the growth of Panax ginseng hairy root culture, and found that the growth rate varied with the inoculum conditions such as the number of root tips, the
length of root tips, the part of root tips, and the inoculum size and age of hairy roots. 相似文献
4.
Jeong Gwi-Taek Park Don-Hee Hwang Baik Park Kyungmoon Kim Si-Wouk Woo Je-Chang 《Applied biochemistry and biotechnology》2002,98(1-9):1115-1127
The growth properties of Panax ginseng hairy roots transformed by Agrobacterium rhizogenes were compared between flask and aerated column or stirred bioreactor. In flask cultures, sucrose, initially 30 g/L, was nearly
exhausted after 45 d of culture. The pH of the medium dropped from 5.5 to 4.96 after 10 d, but afterward it gradually increased
to 6.4. After 45 d, hairy roots grew about 16-folds. The growth rate of hairy roots in air-bubble column or stirred bioreactor
cultures was 1.13 (1.11) to 1.23 (1.20) g fresh wt (dry wt)/(g of cells·d), respectively. For both bioreactors, growth was
about three times as high as in the flask cultivation. 相似文献
5.
H. Moon S. W. Kim J. Lee S. K. Rhee E. S. Choi H. A. Kang I. H. Kim S. I. Hong 《Applied biochemistry and biotechnology》2003,111(2):65-79
As a novel feeding strategy for aptomizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two
substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fedbatch culture
with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h−1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth
rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol
and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L·h), compared with methanol-only feeding. 相似文献
6.
Synthesis of 20S-protopanaxadiol 20-O-β-D-glucopyranoside, a metabolite of Panax ginseng glycosides, and compounds related to it 总被引:1,自引:0,他引:1
A preparative semi-synthetic method was developed to prepare 20S-protopanaxadiol 20-O-β-Dglucopyranoside (1), a metabolite of Panax ginseng glycosides. The 20-O-•-D-glucopyranosides of 20S-hydroxydammar-24-en-3,12-dione, 3β,20S-dihydroxydammar-24-en-12-one, and 3β,12α, 20S-trihydroxydammar-24-ene were synthesized for the first time.
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Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 364–369, July–August, 2006. 相似文献
7.
‘Fufang Danshen tablet’ (FDT), made from Radix salvia miltiorrhiza and Panax notoginseng, is a widely used botanical drug derived from traditional Chinese medicine. Quantification of the active components of Radix salvia miltiorrhiza and Panax notoginseng is very important for regulation of FDT products. In this study HPLC hyphenated with ultraviolet (UV) detection and evaporative
light-scattering detection (ELSD) was used for simultaneous determination of nine active components (three salvianolic acids,
three tanshinones, and three saponins) of FDT products. Separation was performed on a 250 mm × 4.6 mm i.d., 5.0 μm particle-size,
C18 column with linear gradient elution. UV detection at 280 and 254 nm was used for detection of the three salvianolic acids
and the three tanshinones, respectively. ELSD was used for detection of the three saponins, which were difficult to analyze
by use of UV detection. The linearity of the calibration plots was excellent over the concentration ranges investigated (values
of R
2 were >0.99 for all the analytes) and recovery measured at three concentrations was between 92.2 and 107.7%. The validated
method was successfully used for simultaneous determination of these components in FDT products. 相似文献
8.
C. Bailey H. Nicholson P. Morris N. J. Smart 《Applied biochemistry and biotechnology》1985,11(3):207-219
This paper describes the application of a simple log-linear model to suspension cultures of the species Catharanthus roseus
G. Don. Data obtained experimentally have been interpolated by a cubic spline to give data points of regular time period.
A logarithmic transformation has been applied and a linear model fitted to these transformed variables. The results show that
there is a reasonable fit to the experimental data cited.
Using this model a one-step ahead prediction is made for a series of untried experimental variables, and the results generated
are compared with the experimental data subsequently obtained. 相似文献
9.
首次以金银花和乙二胺为原料,通过水热法合成出性能优异的氮掺杂碳点(N-CDs)。制备的N-CDs具有丰富的官能团、良好的水溶性、低细胞毒性、高的荧光稳定性和良好的生物相容性。在最佳条件下,N-CDs能够高选择性地检出Co2+,N-CDs的荧光强度在0.5~3.6 nmol·L-1范围内被Co2+线性猝灭,检出限低至1.38 nmol·L-1,其猝灭机制属于内滤效应和静态猝灭。该方法也已成功应用于实际样品的精确分析。此外,N-CDs还可用于细胞成像及细胞内Co2+传感。 相似文献
10.
Lim JM Kim SW Hwang HJ Joo JH Kim HO Choi JW Yun JW 《Applied biochemistry and biotechnology》2004,119(2):159-170
Optimization of submerged culture conditions for the production of mycelial growth and exopolysaccharides (EPSs) by Collybia maculata was investigated. The optimum temperature and the initial pH for EPS production in a shake-flask culture of C. maculata were found to be 20°C and 5.5, respectively. Among the various medium’s constituents examined, glucose, Martone A-1, K2HPO4, and CaCl2 were the most suitable carbon, nitrogen, and mineral sources for EPS production, respectively. The optimum concentration
of the medium’s ingredients determined using the orthogonal matrix method was as follows: 30 g/L of glucose, 20 g/L of Martone
A-1, 1g/L of K2HPO4, and 1g/L of CaCl2. Under the optimized culture conditions, the maximum concentration of EPSs in a 5-L stirred-tank reactor was 2.4 g/L, which
was approximately five times higher than that in the basal medium. A comparative fermentation result showed that the EPS productivity
in an airlift reactor was higher than that in the stirred-tank reactor despite the lower mycelial growth rate. The specific
productivities and the yield coefficients in the airlift reactor were higher than those in the stirred-tank reactor even though
the volumetric productivities were higher in the stirred-tank reactor than in the airlift reactor. 相似文献
11.
Agar-immobilized alkaliphilic Bacillus sp. AR-009 cells were used for xylanase production using batch and continuous culture. In a batch culture, maximum enzyme
production was observed after 48 h and remained high up to 72 h. In repeated batch cultivation, immobilized cells produced
an appreciable level of xylanase activity in seven consecutive batches without any significant decline in productivity. For
continuous xylanase production, immobilized cells were packed in a jacketed glass column and sterile medium was continuously
pumped. A stable continuous production of xylanase was observed over a period of 1 mo. The volumetric productivity of the
continuous culture was 17-fold higher than the batch culture using free cells. 相似文献
12.
Batch suspension cultures ofCatharanthus roseus G. Don were grown in a 5 L LKB Ultraferm fermenter, converted to operate as an airlift bioreactor, to test the suitability of such
a system for the mass culture of plant cells. Results show that the airlift system has considerable merits as a culture vessel
for such a purpose, including: conversion rates of carbohydrate substrate to cell mass equivalent to > 50% under optimum conditions.
(Operating under these conditions, growth rates of approximately 0.4 d-1 are typical). In the absence of the mechanical shear normally associated with mechanically driven bioreactors, the gently
agitated environment of the airlift vessel proves to be an ideal system for the growth of fragile plant cells. Use of a nozzle
sparger reduces the possibility of a high mass transfer coefficient, except at very high gassing rates, thereby eliminating
any interference with the growth rate caused by high rates of gaseous exchange. 相似文献
13.
Shetty Kalidas Korus roger A. Crawford Dom L. 《Applied biochemistry and biotechnology》1989,(1):825-843
Glycine max was used as a model plant cell suspension culture to establish relationships among growth kinetics, phenolics production,
elicitor action, and peroxidase activity. Timing of elictor addition through monitoring of peroxidase provided an excellent
means of optimizing yields of phenolics and reduced the time span during which phenolics were formed, negating the need for
a secondary production medium. We have also determined that calcium and other cellular effectors like polyamines and organic
osmolytes, when used in conjunction with elicitors, enhance phenolics production. Calcium directly enhanced elicitation, whereas
polyamines and other osmolytes such as glycerol and proline extended cell viability. The study also demonstrated potential
for enhancing secondary metabolite production by a combination of elicitation, cell viability stabilizers, and by addition
of nutrients at the time of elicitation. 相似文献
14.
Theaflavin and its galloyl esters are major polyphenolic pigments of black tea. We compared the efficiency of a variety of oxidizing enzyme systems to synthesize theaflavin and its galloyl esters. Camellia sinensis cell culture efficiently synthesized theaflavin from epicatechin and epigallocatechin with 70% yield and 100% conversion in 4 min. In an administration experiment performed in mice, theaflavin inhibited the increase blood glucose levels in mice that were fed a high-fat diet and subjected to glucose or sucrose loading in mice. 相似文献