Individual variation in the pup attraction call produced by female Australian fur seals during early lactation

Otariid seals (fur seals and sea lions) are colonial breeders with large numbers of females giving birth on land during a synchronous breeding period. Once pups are born, females alternate between feeding their young ashore and foraging at sea. Upon return, both mother and pup must relocate each other and it is thought to be primarily facilitated by vocal recognition. Vocalizations of thirteen female Australian fur seals (Arctocephalus pusillus doriferus) were recorded during the breeding seasons of December 2000 and 2001, when pups are aged from newborns to one month. The pup attraction call was examined to determine whether females produce individually distinct calls which could be used by pups as a basis for vocal recognition. Potential for individual coding, discriminant function analysis (DFA), and classification and regression tree analysis were used to determine which call features were important in separating individuals. Using the results from all three analyses: F0, MIN F and DUR were considered important in separating individuals. In 76% of cases, the PAC was classified to the correct caller, using DFA, suggesting that there is sufficient stereotypy within individual calls, and sufficient variation between them, to enable vocal recognition by pups of this species.     

Age-related differences in the acoustic characteristics of male leopard seals, Hydrurga leptonyx

During the breeding season, the underwater vocalizations and calling rates of adult male leopard seals are highly stereotyped. In contrast, sub-adult males have more variable acoustic behavior. Although adult males produce only five stereotyped broadcast calls as part of their long-range underwater breeding displays the sub-adults have a greater repertoire including the adult-like broadcast calls, as well as variants of these. Whether this extended repertoire has a social function is unknown due to the paucity of behavioral data for this species. The broadcast calls of the sub-adults are less stereotyped in their acoustic characteristics and they have a more variable calling rate. These age-related differences have major implications for geographic variation studies, where the acoustic behavior of different populations are compared, as well as for acoustic surveying studies, where numbers of calls are used to indicate numbers of individuals present. Sampling regimes which unknowingly include recordings from sub-adult animals will artificially exaggerate differences between populations and numbers of calling animals. The acoustic behavior of sub-adult and adult male leopard seals were significantly different and although this study does not show evidence that these differences reflect vocal learning in the male leopard seal it does suggest that contextual learning may be present.     

Perceptual classification based on the component structure of song in European starlings

The ability to recognize individuals based on their vocalizations is common among many species of songbirds. Examining the psychological and neural basis of this functionally relevant behavior can provide insight into the perceptual processing of acoustically complex, real-world, communication signals. In one species of songbird, European starlings (Sturnus vulgaris), males sing long and acoustically complex songs composed of small stereotyped note clusters called motifs. Previous studies demonstrate that starlings are capable of individual vocal recognition, and suggest that vocal recognition results from the association of specific motifs with specific individuals. The present study tests this possibility by examining how variation among the motifs that comprise a song affect its discrimination and classification. Starlings were trained, using operant techniques, to associate multiple songs from a single male starling with one response, and songs from four other male starlings with another response. The level of stimulus control exerted by motif variation was then measured by having subjects classify three sets of novel song bouts in which motifs from the training songs were systematically recombined. The results demonstrate a significant, and approximately linear, relationship between song classification and the relative proportions of familiar motifs from different singers that compose a bout. The results also indicate that the motif proportion effects on song classification are primary to retroactive interference in the recall for specific motifs, and independent of any biases due to the syntactic organization of motifs within a bout. Together, the results of this study suggest that starlings organize the complex vocalizations of conspecifics by memorizing large numbers of unique song components (i.e., motifs) that are then associated with different classes. Because individual starlings tend to possess unique motif repertoires, it is likely that under natural conditions such classes will correspond to individual identity. Thus, it is likely that perceptual processing mechanisms similar to those described by the results of the present study form the basis for individual vocal recognition in starlings.     

Underwater localization of pure tones by harbor seals (Phoca vitulina)

The underwater sound localization acuity of harbor seals (Phoca vitulina) was measured in the horizontal plane. Minimum audible angles (MAAs) of pure tones were determined as a function of frequency from 0.2 to 16 kHz for two seals. Testing was conducted in a 10-m-diam underwater half circle using a right/left psychophysical procedure. The results indicate that for both harbor seals, MAAs were large at high frequencies (13.5 degrees and 17.4 degrees at 16 kHz), transitional at intermediate frequencies (9.6 degrees and 10.1 degrees at 4 kHz), and particularly small at low frequencies (3.2 degrees and 3.1 degrees at 0.2 kHz). Harbor seals seem to be able to utilize both binaural cues, interaural time differences (ITDs) and interaural intensity differences (IIDs), but a significant decrease in the sound localization acuity with increasing frequency suggests that IID cues may not be as robust as ITD cues under water. These results suggest that the harbor seal can be regarded as a low-frequency specialist. Additionally, to obtain a MAA more representative of the species, the horizontal underwater MAA of six adult harbor seals was measured at 2 kHz under identical conditions. The MAAs of the six animals ranged from 8.8 degrees to 11.7 degrees , resulting in a mean MAA of 10.3 degrees .     

Near-threshold equal-loudness contours for harbor seals (Phoca vitulina) derived from reaction times during underwater audiometry: a preliminary study

Equal-loudness functions describe relationships between the frequencies of sounds and their perceived loudness. This pilot study investigated the possibility of deriving equal-loudness contours based on the assumption that sounds of equal perceived loudness elicit equal reaction times (RTs). During a psychoacoustic underwater hearing study, the responses of two young female harbor seals to tonal signals between 0.125 and 100 kHz were filmed. Frame-by-frame analysis was used to quantify RT (the time between the onset of the sound stimulus and the onset of movement of the seal away from the listening station). Near-threshold equal-latency contours, as surrogates for equal-loudness contours, were estimated from RT-level functions fitted to mean RT data. The closer the received sound pressure level was to the 50% detection hearing threshold, the more slowly the animals reacted to the signal (RT range: 188-982 ms). Equal-latency contours were calculated relative to the RTs shown by each seal at sound levels of 0, 10, and 20 dB above the detection threshold at 1 kHz. Fifty percent detection thresholds are obtained with well-trained subjects actively listening for faint familiar sounds. When calculating audibility ranges of sounds for harbor seals in nature, it may be appropriate to consider levels 20 dB above this threshold.     

How to measure information carried by a modulated vocal signature?

Acoustic signaling systems that permit individual recognition are described in an increasing number of species. Evolutionary logic predicts that the efficiency of these signatures is related to the possibilities for confusion. To test this "signature adaptation" hypothesis, one needs a standardized method to estimate and compare the efficiency of different signatures. Beecher [Am. Zool. 22, 477-490 (1989)] developed such a method by comparing scalar parameters extracted from the signals. However, vocal signatures frequently consist in the evolution of one parameter against one other, which are not comparable through Beecher's method. Here we present a method to estimate the efficiency of modulated signatures. A signature's efficiency is given by its information capacity (Hm), derived from Shannon's information theory. The measure of Hm is based on an analysis of variance and uses the Euclidian distances between the signature's contours in the population. To validate our method, simulated datasets of modulated contours were used. The predicted efficiency of those signatures, estimated from Hm, was strongly correlated to its actual efficiency given by two classification methods: a discriminant analysis and a classification by human observers. Being also untied to sample size, Hm therefore allows comparing objectively vocal, but also visual and olfactory signatures.     

Vocal Fatigue—Do Young Speech-Language Pathologists Practice What They Preach?

PurposeSpeech-Language Pathologists can be categorized as Level II professional voice users who play the roles of voice therapist or vocal coach to treat voice problems. SLPs may be at the risk of experiencing vocal fatigue due to vocal loading and other contributing factors. The present study was undertaken to find the percentage of SLPs reporting vocal fatigue, the probable factors resulting in vocal fatigue, the measures that are taken to avoid/reduce the occurrence of vocal fatigue, its effect on their professional and personal life and the measures taken to tackle it.ResultsThe results of this study are based on data collected from 142 SLPs and 50 controls using a questionnaire. 71.13% SLPs reported that they experience vocal fatigue. Some of the contributing factors that are mainly reported are long durations of voice use, voice use for recreational purposes, speaking loudly, frequent throat clearing, lack of adequate hydration and working in noisy or air-conditioned environments. The major symptoms reported were dryness in throat, tightness in neck and shoulder, choking sensation, effortful speech and pain in the throat. 59% SLPs reported that vocal fatigue affected their professional life while 44% SLPs reported that it affects their personal life also to varying degrees. Measures taken to avoid/ reduce the occurrence of vocal fatigue included vocal warm up, break in between sessions, and play activities. Only a few SLPs took intervention measures like ENT consultation, voice therapy, and home remedies.ConclusionSLPs are inevitably at high risk of experiencing vocal fatigue which, if left untreated, will lead to organic voice problems. However, many young SLPs who experience vocal fatigue reported vocal abuse, do not follow vocal hygiene program and do not follow evidence-based preventive or intervention practices to treat vocal fatigue.     

Acoustic correlates of talker sex and individual talker identity are present in a short vowel segment produced in running speech.

Although listeners routinely perceive both the sex and individual identity of talkers from their speech, explanations of these abilities are incomplete. Here, variation in vocal production-related anatomy was assumed to affect vowel acoustics thought to be critical for indexical cueing. Integrating this approach with source-filter theory, patterns of acoustic parameters that should represent sex and identity were identified. Due to sexual dimorphism, the combination of fundamental frequency (F0, reflecting larynx size) and vocal tract length cues (VTL, reflecting body size) was predicted to provide the strongest acoustic correlates of talker sex. Acoustic measures associated with presumed variations in supralaryngeal vocal tract-related anatomy occurring within sex were expected to be prominent in individual talker identity. These predictions were supported by results of analyses of 2500 tokens of the /epsilon/ phoneme, extracted from the naturally produced speech of 125 subjects. Classification by talker sex was virtually perfect when F0 and VTL were used together, whereas talker classification depended primarily on the various acoustic parameters associated with vocal-tract filtering.     

A comparison of vocal tract perturbation patterns based on statistical and acoustic considerations

The purpose of this study was to investigate the relation between vocal tract deformation patterns obtained from statistical analyses of a set of area functions representative of a vowel repertoire, and the acoustic properties of a neutral vocal tract shape. Acoustic sensitivity functions were calculated for a mean area function based on seven different speakers. Specific linear combinations of the sensitivity functions corresponding to the first two formant frequencies were shown to possess essentially the same amplitude variation along the vocal tract length as the statistically derived deformation patterns reported in previous studies.     

Model-based classification of nonstationary vocal fold vibrations

Classification of vocal fold vibrations is an essential task of the objective assessment of voice disorders. For historical reasons, the conventional clinical examination of vocal fold vibrations is done during stationary, sustained phonation. However, the conclusions drawn from a stationary phonation are restricted to the observed steady-state vocal fold vibrations and cannot be generalized to voice mechanisms during running speech. This study addresses the approach of classifying real-time recordings of vocal fold oscillations during a nonstationary phonation paradigm in the form of a pitch raise. The classification is based on asymmetry measures derived from a time-dependent biomechanical two-mass model of the vocal folds which is adapted to observed vocal fold motion curves with an optimization procedure. After verification of the algorithm performance the method was applied to clinical problems. Recordings of ten subjects with normal voice and ten dysphonic subjects have been evaluated during stationary as well as nonstationary phonation. In the case of nonstationary phonation the model-based classification into "normal" and "dysphonic" succeeds in all cases, while it fails in the case of sustained phonation. The nonstationary vocal fold vibrations contain additional information about vocal fold irregularities, which are needed for an objective interpretation and classification of voice disorders.     

Predicted Singers' Vocal Fold Lengths and Voice Classification—A Study of X-Ray Morphological Measures

Students admitted to the solo singing education at the University of Music Dresden, Germany have been submitted to a detailed physical examination of a variety of factors with relevance to voice function since 1959. In the years 1959–1991, this scheme of examinations included X-ray profiles of the singers' vocal tracts. This material of 132 X-rays of voice professionals was used to investigate different laryngeal morphological measures and their relation to vocal fold length. Further, the study aimed to investigate if there are consistent anatomical differences between singers of different voice classifications. The study design used was a retrospective analysis. Vocal fold length could be measured in 29 of these singer subjects directly. These data showed a strong correlation with the anterior-posterior diameter of the subglottis and the trachea as well as with the distance from the anterior contour of the thyroid cartilage to the anterior contour of the spine. These relations were used in an attempt to predict the 132 singers' vocal fold lengths. The results revealed a clear covariation between predicted vocal fold length and voice classification. Anterior-posterior subglottic-tracheal diameter yielded mean vocal fold lengths of 14.9, 16.0, 16.6, 18.4, 19.5, and 20.9 mm for sopranos, mezzo-sopranos, altos, tenors, baritones, and basses, respectively. The data support the assumption that there are consistent anatomical laryngeal differences between singers of different voice classifications, which are of relevance to pitch range and timbre of the voice.     

Pitch (F0) and formant profiles of human vowels and vowel-like baboon grunts: the role of vocalizer body size and voice-acoustic allometry

Key voice features--fundamental frequency (F0) and formant frequencies--can vary extensively between individuals. Much of the variation can be traced to differences in the size of the larynx and vocal-tract cavities, but whether these differences in turn simply reflect differences in speaker body size (i.e., neutral vocal allometry) remains unclear. Quantitative analyses were therefore undertaken to test the relationship between speaker body size and voice F0 and formant frequencies for human vowels. To test the taxonomic generality of the relationships, the same analyses were conducted on the vowel-like grunts of baboons, whose phylogenetic proximity to humans and similar vocal production biology and voice acoustic patterns recommend them for such comparative research. For adults of both species, males were larger than females and had lower mean voice F0 and formant frequencies. However, beyond this, F0 variation did not track body-size variation between the sexes in either species, nor within sexes in humans. In humans, formant variation correlated significantly with speaker height but only in males and not in females. Implications for general vocal allometry are discussed as are implications for speech origins theories, and challenges to them, related to laryngeal position and vocal tract length.     

Acoustic roles of the laryngeal cavity in vocal tract resonance

The acoustic effects of the laryngeal cavity on the vocal tract resonance were investigated by using vocal tract area functions for the five Japanese vowels obtained from an adult male speaker. Transfer functions were examined with the laryngeal cavity eliminated from the whole vocal tract, volume velocity distribution patterns were calculated, and susceptance matching analysis was performed between the laryngeal cavity and the vocal tract excluding the laryngeal cavity (vocal tract proper). It was revealed that the laryngeal cavity generates one of the formants of the vocal tract, which is the fourth in the present study. At this formant, the resonance of the laryngeal cavity (the 1/4 wavelength resonance) induces the open-tube resonance of the vocal tract proper (the 3/2 wavelength resonance). At the other formants, on the other hand, the vocal tract proper acts as a closed tube, because the laryngeal cavity has only a small contribution to generating these formants and the effective closed end of the whole vocal tract is the junction between the laryngeal cavity and the vocal tract proper.     

Supraglottic contribution to voice quality

Although there has been continuing interest in voice quality, much of this research has focused on the vocal folds rather than the supraglottal structures. This paper reports the use of videoendoscopy for studying supraglottal participation in various singing tasks. In a preliminary study presented last year by the present authors, CT scanning was used to corroborate videoendoscopic observation. Vocal tract activities observed included variation of laryngeal height with pitch, variation of pharyngeal wall dimension with pitch and vowel, and marked supraglottic constriction with certain vocal imitations. In order to gain a better understanding of vocal training, and its effect upon vocal tract physiology, a study was designed using videoendoscopy to observe singers with significant experience and training while performing various vocal tasks. The tasks focused on the following: (1) vocal tract activity associated with pitch changes; (2) the physiology involved in the production of “cover”; (3) the structures involved in the production of vibrato; and (4) the physiology of the singer's “ring.” It would appear that videoendoscopy will become increasingly more valuable to the voice community as our understanding of vocal tract physiology improves.     

Toward the quantification of vocal efficiency

Quantifiable aspects of vocal fold vibration may be inferred by means of the electrolaryngograph. Changes in inferred vocal fold closed quotient are considered as a possible correlate of acoustic efficiency variation; automatic measures of closed quotient compare favorably with results obtained from inverse filtering of the speech pressure waveform. This article describes closed quotient measures based on electrolaryngographic analysis of 18 trained and untrained men singers, and results show a significant difference in mean vocal fold closed quotient between trained and untrained singers.     

Complications of collagen injection of the vocal fold: report of several unusual cases and review of the literature

Injection laryngoplasty is one of the most frequently performed procedures in patients with voice complaints. Various biomaterials have been used to medialize vocal folds or to treat symptoms of vocal fold scar. The ideal biomaterial would be easily injected through a fine-gauge needle, well tolerated, and long lasting. Injectable collagen preparations fulfill at least two of these criteria, and collagen has been used widely for vocal fold injections. MATERIALS AND METHODS: We present a retrospective review of two unusual complications of collagen injection and a review of the relevant literature on the complications of medical use of collagen compounds. RESULTS: Two patients in whom collagen was injected formed firm submucosal deposits that interrupted the normal mucosal wave and produced significant dysphonia. Surgical removal of these deposits restored the mucosal wave and improved voice quality. Management of this unusual complication of human collagen injection in the vocal fold has not been reported previously. Other complications of collagen injection include hypersensitivity reactions to bovine collagen, local abscess formation at injection sites, and possibly induction of collagen vascular disease in some patients. CONCLUSIONS: Although collagen injections of the vocal fold rarely result in complications, physicians using collagen must be familiar with the types of complications that can occur. Proper diagnosis and prompt management of complications can result in good outcomes.     

Phono-articulatory variations of women in reproductive age and postmenopausal

Human verbal expression requires coordinated interaction among cortical, neuromuscular, and peripheral events. It is affected by hormonal factors and shows different characteristics in reproductive age and postmenopausal women. The present study compared phono-articulatory characteristics between women in reproductive age postmenopausal. Acoustic variations in fundamental frequency, voice sustenance, formants, vocal intensity, and verbal diadochokinesis were measured. Forty-five women in reproductive age with regular menstrual cycles and taking no hormonal contraceptives and 45 postmenopausal women receiving no hormonal replacement therapy for at least 3 years were interviewed, and their verbal productions were tape-recorded. Acoustic analyses were performed using the Kay Elemetrics Motor Speech Profile (Lincoln Park, NJ). Student t test was employed to compare data between the two groups when they presented normal distribution and Mann-Whitney test when they were asymmetrical. Results showed a greater variability in vocal sustenance, less variation in formants, and verbal diadochokinesis in postmenopausal women. There were no significant variations in fundamental frequency and vocal intensity. These results emphasize the need of a multidisciplinary integrated research, when assessing phono-articulatory processes after the menopause. A better understanding in this field will make it possible to elaborate strategies to offer a better life quality for postmenopausal women.     

Modeling the role of nonhuman vocal membranes in phonation

Although the mammalian larynx exhibits little structural variation compared to sound-producing organs in other taxa (birds or insects), there are some morphological features which could lead to significant differences in acoustic functioning, such as air sacs and vocal membranes. The vocal membrane (or "vocal lip") is a thin upward extension of the vocal fold that is present in many bat and primate species. The vocal membrane was modeled as an additional geometrical element in a two-mass model of the larynx. It was found that vocal membranes of an optimal angle and length can substantially lower the subglottal pressure at which phonation is supported, thus increasing vocal efficiency, and that this effect is most pronounced at high frequencies. The implications of this finding are discussed for animals such as bats and primates which are able to produce loud, high-pitched calls. Modeling efforts such as this provide guidance for future empirical investigations of vocal membrane structure and function, can provide insight into the mechanisms of animal communication, and could potentially lead to better understanding of human clinical disorders such as sulcus vocalis.