共查询到8条相似文献,搜索用时 15 毫秒
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以铂电极支撑的磷脂双层膜(Supported Bilayer Lipid Membrane,s-BLM)作为生物膜的模型,以Fe(CN)36-和Fe(CN)64-为探针分子,利用循环伏安法(CV)和交流阻抗谱(EIS)研究两性表面活性剂十二烷基磺基甜菜碱(Dodecyl Sulfobetaine,DSB)对s-BLM相互作用。结果显示,DSB可以嵌入到s-BLM的疏水区,容易使其表面分子的排列发生变化,产生缺陷或孔洞,探针分子Fe(CN)63-和Fe(CN)64-可以通过这些微孔接近电极,产生氧化还原响应。并且作用时间、DSB的浓度以及胆固醇的存在与否对二者的相互作用有直接影响。此外作用后的双层膜在0.1mol/LKCl溶液中能够自我修复,这表明DSB与s-BLM的相互作用是可逆的。 相似文献
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以支撑磷脂双层膜(supported bilayer lipid membrane, s-BLM)作为生物膜模型, 采用循环伏安法和交流阻抗技术研究了脱氧胆酸钠(sodium deoxycholate, NaDC)与s-BLM的相互作用. 结果表明, NaDC能降低磷脂分子的有序性, 诱发s-BLM上形成孔洞或缺陷, 并且它们之间的这种相互作用对作用时间、NaDC溶液的浓度和pH值以及胆固醇的存在与否具有依赖性, 并且作用后的s-BLM在0.1 mol/L的KCl溶液中能够自我修复, 这表明NaDC与s-BLM的相互作用是可逆的. 相似文献
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《Electroanalysis》2018,30(2):207-219
Biological membranes are essential parts of living systems. They represent an interface between intracellular and extracellular space. Depending on their structure, they often perform very complex functions and play an important role in the transport of both charged and uncharged particles in any organism. Structure of the biological membranes, which play very important role in electrochemical processes inside living organisms, is very complicated and still not precisely defined and explained. Model lipid membranes are used to gain detail information about properties of real biological membranes and about associated electrochemical processes. Electrochemistry, especially electrochemical impedance spectroscopy (EIS), can play a useful role in the characterization of properties of model lipid membranes (planar and supported lipid bilayers, tethered lipid membranes, liposomes, etc.). This review is focused on model biological membranes and the possibilities and limitations of electrochemical methods and namely of EIS in this field. 相似文献
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Indr Aleknavi
ien Martynas Talaikis Rima Budvytyte Gintaras Valincius 《Molecules (Basel, Switzerland)》2021,26(22)
Tethered bilayer lipid membranes (tBLMs) have been known as stable and versatile experimental platforms for protein–membrane interaction studies. In this work, the assembly of functional tBLMs on silver substrates and the effect of the molecular chain-length of backfiller molecules on their properties were investigated. The following backfillers 3-mercapto-1-propanol (3M1P), 4-mercapto-1-butanol (4M1B), 6-mercapto-1-hexanol (6M1H), and 9-mercapto-1-nonanol (9M1N) mixed with the molecular anchor WC14 (20-tetradecyloxy-3,6,9,12,15,18,22 heptaoxahexatricontane-1-thiol) were used to form self-assembled monolayers (SAMs) on silver, which influenced a fusion of multilamellar vesicles and the formation of tBLMs. Spectroscopic analysis by SERS and RAIRS has shown that by using different-length backfiller molecules, it is possible to control WC14 anchor molecules orientation on the surface. An introduction of increasingly longer surface backfillers in the mixed SAM may be related to the increasing SAMs molecular order and more vertical orientation of WC14 at both the hydrophilic ethylenoxide segment and the hydrophobic lipid bilayer anchoring alkane chains. Since no clustering of WC14 alkane chains, which is deleterious for tBLM integrity, was observed on dry samples, the suitability of mixed-component SAMs for subsequent tBLM formation was further interrogated by electrochemical impedance spectroscopy (EIS). EIS showed the arrangement of well-insulating tBLMs if 3M1P was used as a backfiller. An increase in the length of the backfiller led to increased defectiveness of tBLMs. Despite variable defectiveness, all tBLMs responded to the pore-forming cholesterol-dependent cytolysin, vaginolysin in a manner consistent with the functional reconstitution of the toxin into phospholipid bilayer. This experiment demonstrates the biological relevance of tBLMs assembled on silver surfaces and indicates their utility as biosensing elements for the detection of pore-forming toxins in liquid samples. 相似文献
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Aybüke A. İsbir Turan Zafer Üstündağ Ali Osman Solak Emine Kılıç Ayça Avseven 《Electroanalysis》2008,20(15):1665-1670
This work describes the characterization of the grafted 2‐benzo[c]cinnoline (2BCC) molecules at a glassy carbon (GC) electrode surface by voltammetry and spectroscopy. Attachment of the molecule to the carbon substrate was achieved by the electrochemical reduction of 2‐benzo[c]cinnoline diazonium salt (2BCC‐DAS). GC electrode modification was carried out in aprotic solution with 2BCC diazonium salt. Dopamine (DA) and ascorbic acid (AA) were used to prove the surface modification to see the blockage of the electron transfer. The presence of 2BCC at the GC electrode surface was characterized by cyclic voltammetry and Raman spectroscopy. Raman spectroscopy was used to monitor molecular bound properties of the adsorbates at the 2BCC‐GC surface and confirm the attachment of 2BCC molecules onto the GC surface. The thickness of the 2BCC film on GC was also investigated by ellipsometric measurement. 相似文献