高效液相色谱梯度洗脱法测定唐威胶囊中的盐酸小檗碱及葛根素

 采用反相高效液相色谱梯度洗脱法,用ODS柱,以甲醇-醋酸-三乙胺-水为流动相,在230 nm处同时测定唐威胶囊中盐酸小檗碱及葛根素的含量。平均添加回收率(n=5)和RSD值分别为98.2%和1.6%(盐酸小檗碱);97.5%和1.8%(葛根素)。 关键词：     

高效液相色谱法测定葛粉保健品中葛根素的含量

对不同产区葛粉保健品中葛根素的含量进行分析,拟建立高效液相色谱法快速测定葛粉保健品中葛根素含量的方法.固定相为Agilent-C18柱(4.6 mm×300 mm,5μm),流动相为甲醇一水(70 30,体积比)溶液,流速为1.0 mL·min-1,波长为250 nm,进样量10μL.结果表明:葛根素的质量浓度在10.00～100.00 mg·L-1范围内与峰面积呈线性关系,加标回收率为99.0%～99.8%,检出限(3S/N)为1.0 mg·L-1,相对标准偏差分别为0.57%,0.64%,1.71%.     

单柱离子色谱法测定一价阳离子的流动相研究

对单柱离子色谱法测定一价阳离子的流动相进行了系统研究，阐述了一价阳离子的保留行为和电导检测行为与流动相之间的关系，分别对无机酸（硝酸）、有机酸（柠檬酸）和芳香碱（苯胺）为流动相测定一价阳离子进行了讨论，其中有机酸和无机酸是较为适宜的流动相。     

反相高效液相色谱法测定葛根素和大豆苷元

利用反相高效液相色谱法,配以二极管阵列检测器测定了心可舒片中的葛根素和大豆苷元,建立了一种心可舒片的质量控制方法。色谱柱为C18柱,流动相为乙腈和0．02mol／L磷酸二氢钾,柱温为25℃,流速为0．8mL／min,梯度洗脱,检测波长为250nm。葛根素和大豆苷元的平均回收率分别为97．3％和101．4％：RSD为0．6％和1．9％。     

单柱离子色谱法测定一价阳离子的流动相研究

 对单柱离子色谱法测定一价阳离子的流动相进行了系统研究，阐述了一价阳离子的保留行为和电导检测行为与流动相之间的关系，分别对无机酸（硝酸）、有机酸（柠檬酸）和芳香碱（苯胺）为流动相测定一价阳离子进行了讨论，其中有机酸和无机酸是较为适宜的流动相。     

流动注射双安培法测定葛根素

利用不可逆电对的双安培检测原理,建立了流动注射双安培直接检测葛根素的电化学新方法.使用经过恒电位预阳极化处理的双铂电极,通过耦合葛根素在一支电极上的氧化和氧化铂在另一支电极上的还原两个不可逆电极过程,构成流动注射双安培检测体系.在外加电位差为0.2 V时,pH 8.94的碳酸钠-碳酸氢钠缓冲溶液中,测得氧化电流与葛根素浓度在6.0×10-6～1.0×10-3 mol/L范围内呈线性关系(r=0.997 4,n=8),检出限为1.0×10-6 mol/L.连续40次测定6.0 ×10-4 mol/L的葛根素,RSD=1.58%.用该方法对葛根素含量进行了测定,结果令人满意.     

反相高效液相色谱法测定3种中成药中的葛根素

 建立了测定小儿清感灵片、步长新脑心通胶囊和感冒清热颗粒 3种中成药中葛根素含量的反相高效液相色谱方法。采用APEXODS色谱柱 ,以醋酸铵缓冲液 (10 0mmol/L ,pH 5 0 ) 甲醇 (体积比为 75∶2 5 )的混合溶液为流动相 ,检测波长为 2 5 0nm ,流速为 0 8mL/min。葛根素在 2mg/L～ 2 0mg/L时其色谱峰面积与质量浓度的线性关系良好 (r =0 9999) ;上述 3种中成药中的葛根素含量分别为 3 48mg/g ,1 0 8mg/ g及 1 5 2mg/ g(蔗糖型 ) ;其加样回收率分别为 99 0 % ,93 4%和 97 5 %。该法简便、快速、专属性强 ,可以作为多种中药制剂中葛根素含量的测定方法。     

高效液相色谱法测定葛根芩连片中的葛根素

建立高效液相色谱测定葛根芩连片中葛根素含量的方法。以体积分数50%的甲醇为提取液对样品超声提取20 min,采用DiamonsilTMC18(250 mm×4.6 mm,5μm)色谱柱,以甲醇–乙腈–水(体积比8∶12∶80)为流动相,流速为1.0 mL/min,检测波长为250 nm,柱温为30.0℃,进样量体积10μL。在最佳实验条件下,葛根素与其它物质能完全分离,葛根素的质量浓度在5.43~543.2μg/mL范围内与色谱峰面积呈良好的线性,线性相关系数r=0.999 9,方法检出限为3.50μg/mL(S/N=3)。方法加标回收率为100.0%,测定结果的相对标准偏差为1.6%(n=6)。该方法简单、快速、重现性好,适用于葛根芩连片中葛根素的测定。     

加压毛细管电色谱测定葛根中三种异黄酮类化合物

建立了加压毛细管电色谱(pCEC)检测葛根中异黄酮类化合物葛根素、大豆苷、大豆苷元的方法。采用C18毛细管色谱柱,以NaH2PO4缓冲盐水溶液和甲醇为流动相,优化流动相比例、流动相流速、NaH2PO4缓冲盐水溶液浓度和pH、分离电压等色谱条件。结果表明,在流动相为17.5 mmol/L NaH2PO4缓冲盐水溶液(pH 4.0):甲醇=55:45(V/V),分离电压3 kV,流动相流速80μL/min,检测波长250 nm的条件下,葛根素、大豆苷、大豆苷元质量浓度在200～1000μg/mL范围内线性关系良好,相关系数在0.9960～0.9982之间,平均回收率在98.6%～100.9%之间,RSD为3.1%～3.5%之间。该方法已用于葛根中异黄酮类物质的分离检测。     

单柱离子色谱同时测定碱土金属和过渡金属离子的研究

本文用单柱离子色谱法，以乙二胺柠檬酸作流动相，同时分离和测定１１种金属离子。探讨了离子的保留行为与流动相浓度及ＰＨ值之间的关系，选择了最佳色谱分离条件，用于人发试样的分析，得到满意结果，该法具有简便，快速、准确等优点。     

A New Pueroside from Pueraria lobata

Chemistry of Natural Compounds - A new constituent, named (4R)-6′′′′ -O-sinapoylpueroside B (1), was isolated from the dried roots of Pueraria lobata (Willd.) Ohwi. The...     

In der Knolle von Pueraria tuberosa

Ohne Zusammenfassung     

A new isoflavone glycoside from Pueraria alopecuroides

A new isoflavone glycoside, (?)-tuberosin-3-O-β-D-glucopyranoside (1), along with 10 known compounds 1a-10, was isolated from Pueraria alopecuroides. Their structures were determined on the basis of spectral data including 1D and 2D NMR and HREIMS. These compounds were isolated from this plant for the first time.     

Flavonoids from the Flowers of Pueraria Lobata

A new isoflavonoid glycoside, gehuain, together with three flavonoids and eight isoflavonoids, was isolated from the flowers of Pueraria lobata. Three of them, apigenin, apigenin 4′‐O‐β‐D‐glucoside, and wistin, were reported the first time from this plant. Their structures were established on the basis of spectral evidence.     

A new isoflavonolignan from Pueraria alopecuroides Craib

A new isoflavonolignan has been isolated from the Pueraria alopecuroides Craib. The structure was elucidated by the combination of 1D, 2D NMR analysis and mass spectrometry.     

粉葛淀粉的理化特性

粉葛淀粉的理化特性;葛淀粉;糊化特性;X射线衍射;流变     

Two New Oleanene-Type Triterpenoid Saponins from Pueraria peduncularis

Pueraria pedllncltlaris Grab. (Leguminosae) is a plant growing in the southwest of China.The plants of Pueraria DC. are important oriental crude drugs used as a perspiration,antipyretic and antispasmodic agents. Various isoflavonoids and triterpenoidsaponins"' have been discovered from them. Since P pedunculariS is toxic. it hasnever been used as a source for medicines. There are only scarce reports on its chemicalconstituents.In the preceding paper', we reported the isolation of a new …     

Antitumor activity of spinasterol isolated from Pueraria roots

We purified phytoestrogens from Pueraria root (Pueraria mirifica from Thailand and Pueraria lobata from Korea), which is used as a rejuvenating folk medicine in Thailand and China. Dried, powdered plant material was extracted with 100% ethanol and further separated by concentration, filtration, and thin layer silica gel chromatography. Using the fractions obtained during separation, we first investigated their cytotoxicity in several cancer cell lines from various tissues. The ethanol-extracted components (PE1, PE4) had significant antiproliferative effects on breast cancer cell lines, including MCF-7, ZR-75-1, MDA-MB-231, SK-BR-3, and Hs578T. Second, we compared these results with the cytotoxic effects of known flavonoids, sterols, and coumarins from Pueraria root. The known compounds were not as effective, and occurred in a different polarity region on HPLC. Third, further separation resulted in the isolation of eight different components (Sub PE-A to -H). One of these, PE-D, affected the growth of some breast cancer cell lines (MCF-7, MDA- MB-231) in a dose- and time-dependent manner, as well as the growth of ovarian (2774) and cervical cancer cells (HeLa). Finally, a transfection assay showed that this component had an estrogenic effect similar to 17beta - estradiol, which activates both estrogen receptor alpha (ERalpha) and ERbeta. The NMR analysis determined that spinasterol (stigmasta-7, 22-dien-3beta-ol) is an active cytotoxic component of Pueraria root.     

Peduncin, A New Indole Alkaloid from Pueraria peduncualris

Pueraria peduncularis (Grah. ex Benth.) Benth. is a plant of Leguminosae which grows in the southwest of China. Different from other plants of Pueraria, it could not be used as a medicine due to its toxicity1. In order to use the Pueraria plants safer, we studied the constituents of P. peduncularis. Previous phytochemical studies of an n-BuOH extract afforded three triterpenoid saponins2-3. The continuing investigation on CHCl3 extract led to the isolation of a new indole alkaloid. …     

New Isoflavone C‐Glycosides from Pueraria lobata

Three new isoflavone C‐glycosides, along with two known isoflavone O‐glycosides, were isolated from the roots of Pueraria lobata (Willd .) Ohwi . The structures of the new compounds were elucidated as 4′,7‐dihydroxy‐3′‐methoxyisoflavone 8‐C‐[β‐d‐ glucopyranosyl‐(1→6)]‐β‐d‐ glucopyranoside ( 1 ), 4′,7‐dihydroxy‐3′‐methoxyisoflavone 8‐C‐[β‐d‐ apiofuranosyl‐(1→6)]‐β‐d‐ glucopyranoside ( 2 ), and 8‐C‐β‐d‐ glucopyranosyl‐4′,7‐dihydroxy‐3′‐methoxyisoflavone 4′‐O‐β‐d‐ glucopyranoside ( 3 ) on the basis of spectroscopic methods, especially 2D‐NMR and MS analyses. The known compounds isolated were identified by comparison of their physical and spectroscopic data with those reported in the literature.