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本文建立了高效液相色谱法(HPLC)测定千金止带丸中芍药苷含量的方法。采用Reliasil C18柱(150×4.6 mm i.d., 5μm),以乙腈水(12∶88,V/V)为流动相,在流速0.8 mL/min,检测波长230 nm,柱温40℃条件下,芍药苷在0.139 8~0.699 0μg范围内具有良好的线性关系,r =0.999 9,平均回收率为99. 09%, RSD = 0. 91%(n=5)。本法简便,准确,灵敏度高,重复性好,可用于该药品成分的含量测定。 相似文献
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HPLC法测定百合固金颗粒中芍药苷和甘草苷 总被引:2,自引:0,他引:2
建立同时测定百合固金颗粒中芍药苷和甘草苷含量的高效液相色谱法。采用Welch Ultimate XB–C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈–0.1%磷酸(体积比为14∶86),流速为1.0 m L/min,柱温为30℃,检测波长为230 nm。百合固金颗粒中芍药苷在14.91~149.12 ng,甘草苷在34.75~347.52 ng范围内均有良好线性,线性相关系数分别为0.999 5,0.999 2,平均回收率分别为96.7%,95.9%,测定结果的相对标准偏差小于3%(n=6)。该方法操作简单,专属性强,准确度高,重复性好,可以有效地控制百合固金颗粒的质量。 相似文献
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Qiao Wang Rongxia Liu Hongzhu Guo Min Ye Changhong Huo Kaishun Bi Dean Guo 《Chromatographia》2005,62(11-12):581-588
A reversed phase high performance liquid chromatography method was established for the simultaneous determination of eight major constituents, namely gallic acid, paeoniflorin sulfonate, catechin, albiflorin, paeoniflorin, benzoic acid, pentagalloylglucose and benzoylpaeoniflorin in red and white peony roots, the two commonly used traditional Chinese medicinal herbs. The optimal conditions of separation and detection were achieved on a C18 analytical column with a gradient mobile phase consisting of acetonitrile and 0.015% phosphoric acid at the flow rate of 1.0 mL min?1 and detection wavelength set at 230 nm. All calibration curves showed good linear regression (r>0.9995) within test ranges. This method provided good reproducibility with overall intra-and inter-day precision of less than 5% and 4% and good accuracy with recovery of more than 93%, respectively. The method was successfully applied to determine 71 samples of red and white peony roots collected from different areas. The results indicated that the contents of eight compounds varied significantly among the samples determined, which mainly resulted from processing procedure and habitat variation. The roots of Paeonia veitchii Lynch. contained a much higher amount of gallic acid and pentagalloylglucose than that of Paeonia lactiflora Pall., which could be used to distinguish the two similar species. 相似文献
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针对采用团体标准T/CBMF 49-2019&T/CWA 301-2019测定沥青基耐根穿刺防水卷材中聚合物类阻根剂含量分析时间长(约12 h)、检出率低等问题,提出了题示方法。取1 g样品,加入25 mL甲基叔丁基醚,在室温下超声溶解30 min后加入25 mL乙醇,超声萃取15 min,用0.45μm疏水聚四氟乙烯(PTFE)滤头过滤,用高效液相色谱法(HPLC)测定滤液中游离4-氯-2-甲基苯氧基丙酸(MCPP酸)质量分数(w游)。另取1 g样品,加入甲基叔丁基醚25 mL,在室温下超声溶解30 min,加入含0.1 mol·L-1氢氧化钠的乙醇溶液25 mL,继续超声15 min,取出后于40℃水浴中皂化水解1 h,用0.45μm疏水PTFE滤头过滤,用HPLC测定滤液中总MCPP酸质量分数(w总)。在HPLC分析时,流动相采用0.5%(体积分数)磷酸溶液-乙腈体系,以w总-w游/0.6计算聚合物类阻根剂的含量。结果表明:方法的分析时间可缩短至约3 h,MCPP酸标准曲线的线性范围为5~100 mg·L-1,检出限(3S/N)为0.03‰。方法用于来自不同厂家的不同类型样品的分析,测定值和产品标签值基本一致,测定值的相对标准偏差(n=6)均小于2.0%,加标回收率为95.1%~108%。 相似文献
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环境水体中亚硝态氮、硝态氮和总氮的液相色谱测定 总被引:2,自引:0,他引:2
建立了环境水体中NO2-、NO3-及总氮含量的液相色谱测定方法.采用Hypersil ODS(5μm,250mm×4.6mm i.d.)色谱柱;流动相:17.5mmoL/L KH2PO4-2mmol/L H3PO4缓冲液(pH3.5)-乙腈(体积比92.5:7.5);流速:0.8mL/min;柱温:30℃;紫外检测器:波长204nm.结果表明:水体中NO2-和NO3-的线性范围(以N计):1~80ng,r=0.999 9;方法检出限:NO2-0.4ng、NO3-0.09ng;回收率为NO2-99.2%~102.4%、NO3-98.7%~99.3%,RSD为0.79%和0.25%. 相似文献
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A capillary zone electrophoretic method has been developed for the quantitative analysis of five phenolic glucosides, 6′-O-vanilloylarbutin (VA), 7-O-feruloylorientin (FE), lutonarin (LN), isoorient (IO) and luteolin (LL), in Gentiana piasezkii with UV detection at 270 nm. 7-O-β-D-glucosyl-coumarin was selected as the internal standard. The applied voltage was 15 kV and the capillary temperature was kept constant at 25 °C. The effect of pH, the concentration of methanol and boric acid on migration were studied systematically. Optimum separation was achieved with 200 mM boric acid buffer at pH 9.50 containing 10% (v/v) methanol. Regression equations revealed good linear relationship between the peak area ratio of each compound and internal standard and its concentration. The correlation coefficients were 0.9975, 0.9997, 0.9998, 0.9998 and 0.9988 for VA, FE, LN, IO and LL, respectively. The relative standard deviations of migration time and the peak area ratio of each analyte and internal standard were <1.78% and 4.93%, respectively. The contents of the five compounds in Gentiana piasezkii were successfully determined with satisfactory repeatability and recovery. 相似文献
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以甲醇-水(95:5,V/V)为流动相,用ODS柱以高效液相色谱法测定非诺贝特含量。紫外检测波长为286nm。非诺贝特在浓度为0.04-0.20g/L间线线性关系良好。重复进样RSD=0.14%,最低检出浓度为0.10mg/L,平均回收率为99.66%。 相似文献
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《理化检验(化学分册)》2015,(9)
提出了一种测定氮丙啶的高效液相色谱分析方法。将自制氮丙啶单体与福林试剂(1,2-萘醌-4-磺酸钠盐)反应得到衍生物,通过NMR和IR表征验证衍生物结构。衍生物在Agilent ODB C18色谱柱(250mm×4.6mm,5μm)上分离,以乙腈-0.04mol·L-1磷酸氢二钠混合溶液为流动相进行梯度洗脱,检测波长为258nm。氮丙啶的线性范围为1.00~20.0mg·L-1,检出限(3S/N)为0.05mg·L-1,测定值的相对标准偏差(n=6)小于2%。 相似文献
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提出了高效液相色谱法测定苯并三氮唑产品以及工业循环冷却水中苯并三氮唑含量的方法。色谱分离采用Kromasil C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水(25+75)为流动相,流量为1.0 mL.min-1,检测波长为260 nm。苯并三氮唑的质量浓度在3.84~76.8 mg.L-1范围内与峰面积呈线性关系,平均回收率为96.4%。 相似文献
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饲料中生物素的高效液相色谱测定 总被引:10,自引:0,他引:10
介绍了用液相色谱测定饲料中生物素的方法,探讨了流动相中缓冲液、pH值、有机溶剂等对分离的影响,确定的较佳色谱条件:HypersilODS柱,流动相为甲醇-0.1mol·L-1KH2PO4(H3PO4调pH=3.5)(体积比25∶75),流速1.0mL/min,紫外210nm检测;方法的相对标准偏差在3.5%以内,回收率在92%~102%,检出限为1mg/kg;实验表明该法简便、快速,适应性好;方法应用于饲料样品中生物素的测定,取得了很好的结果。 相似文献
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Utha Hellmann Ingo Lüderwald Monika Neuhäuser 《Fresenius' Journal of Analytical Chemistry》1986,325(3):290-292
Summary The determination of N-acetyl-l-glutamine (AC-GLN) in urine of rats after derivatization with xanthydrol by HPLC is described. The urine samples were collected from two groups of rats, one group fed normal nutrition, the other treated with AC-GLN. About 5 mM/l AC-GLN was detected in urine of untreated animals, whereas the excretion of the other group was in the range of 50 mM/l corresponding to about 50 wt.-% of the given AC-GLN.
Bestimmung von N-Acetyl-l-glutamin in Urin mit Hilfe der HPLC
Zusammenfassung Ein Verfahren wird beschrieben zur HPLC-Bestimmung von N-Acetyl-l-glutamin (AC-GLN) in Rattenurin nach Derivatisierung mit Xanthydrol. Die Urinproben wurden von zwei Gruppen von Ratten gesammelt, einer Gruppe mit normaler Ernährung und einer Gruppe mit AC-GLN-Behandlung. Im Urin der unbehandelten Tiere wurden 5 mM/l AC-GLN nachgewiesen; die Ausscheidung bei der anderen Gruppe lag im Bereich von 50 mM/l, entsprechend etwa 50 Gew.% des eingesetzten AC-GLN.相似文献
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高效液相色谱法测定葛粉保健品中葛根素的含量 总被引:2,自引:0,他引:2
孙林超 《理化检验(化学分册)》2008,44(10):945-946,949
对不同产区葛粉保健品中葛根素的含量进行分析,拟建立高效液相色谱法快速测定葛粉保健品中葛根素含量的方法.固定相为Agilent-C18柱(4.6 mm×300 mm,5μm),流动相为甲醇一水(70 30,体积比)溶液,流速为1.0 mL·min-1,波长为250 nm,进样量10μL.结果表明:葛根素的质量浓度在10.00~100.00 mg·L-1范围内与峰面积呈线性关系,加标回收率为99.0%~99.8%,检出限(3S/N)为1.0 mg·L-1,相对标准偏差分别为0.57%,0.64%,1.71%. 相似文献