Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation

We have investigated the inactivation of Saccharomyces cerevisiae (yeast cells) by ultrasonic irradiation. The amplitude on the vibration face contacting the sample solution was used as an indication of the ultrasonic power intensity. The effects of the amplitude on the vibration face and the initial cell numbers on the sonolytic inactivation of yeast cells have been investigated using a horn-type sonicator (27.5 kHz). The inactivation of the yeast cells by ultrasonic irradiation shows pseudo first-order behavior. The inactivation rate constant varied from 0.0007 to 0.145 s(-1) when the amplitude on the vibration face was in the range of 1-7 microm(p-p). The change in the inactivation rate constant as a function of the amplitude on the vibration face was similar to that of the OH radical formation rate under the same conditions. The threshold of this sonicator was 3 microm(p-p) with the amplitude on the vibration face. The initial cell numbers (from 10(2) to 10(5) mL(-1)) had an influence on the inactivation of the yeast cells by ultrasonic irradiation. The inactivation rate constants varied from 0.023 to 6.4 x 10(-3) s(-1), and the inactivation by ultrasonic irradiation was fastest at the lowest initial cell numbers. In a squeeze-film-type sonicator (26.6 kHz), 90% inactivation of the yeast cells was achieved by ultrasonic irradiation for 60 min.     

Ultrasonic butt welding of aluminum,aluminum alloy and stainless steel plate specimens

Welding characteristics of aluminum, aluminum alloy and stainless steel plate specimens of 6.0 mm thickness by a 15 kHz ultrasonic butt welding system were studied. There are no detailed welding condition data of these specimens although the joining of these materials are required due to anticorrosive and high strength characteristics for not only large specimens but small electronic parts especially. These specimens of 6.0 mm thickness were welded end to end using a 15 kHz ultrasonic butt welding equipment with a vibration source using eight bolt-clamped Langevin type PZT transducers and a 50 kW static induction thyristor power amplifier. The stainless steel plate specimens electrolytically polished were joined with welding strength almost equal to the material strength under rather large vibration amplitude of 25 microm (peak-to-zero value), static pressure 70 MPa and welding time of 1.0-3.0 s. The hardness of stainless steel specimen adjacent to a welding surface increased about 20% by ultrasonic vibration.     

近场超声悬浮启浮瞬态行为的理论分析及实验测试

超声悬浮气膜瞬态行为的认识对悬浮体运动规律及悬浮精度控制等都有着重要作用。运用气体动力学理论研究超声挤压问题,建立了自由悬浮体瞬态气膜压力及其非线性动力学模型.应用数值方法对模型进行求解,获得超声激励下气体挤压膜在启浮过程中的瞬态压力分布、承载能力以及悬浮盘位移的变化规律。分析发现激励盘振幅、频率等参数对启浮过程的瞬态行为有着显著的影响,激励盘振幅越小、激励频率越高,启浮过渡过程达到稳态所需时间越长。在理论分析的基础上还对瞬态启浮过程进行实验研究,应用激光位移传感器对瞬态悬浮位移进行测试,发现理论分析和实验测试结果一致性较好。      

Vibration characteristics of aluminum surface subjected to ultrasonic waves and their effect on wetting behavior of solder droplets

The vibration characteristics of an aluminum surface subjected to ultrasonic waves were investigated with a combination of numerical simulation and experimental testing. The wetting behavior of solder droplets on the vibrating aluminum surface was also examined. The results show that the vibration pattern of the aluminum surface is inhomogeneous. The amplitude of the aluminum surface exceeds the excitation amplitude in some zones, while the amplitude decreases nearly to zero in other zones. The distribution of the zero-amplitude zones is much less dependent on the strength of the vibration than on the location of the vibration source. The surface of the liquid solder vibrates at an ultrasonic frequency that is higher than the vibration source, and the amplitude of the liquid solder is almost twice that of the aluminum surface. The vibration of the surface of the base metal (liquid solder) correlates with the oxide film removal effect. Significant removal of the oxide film can be achieved within 2 s when the amplitude of the aluminum surface is higher than 5.4 μm or when the amplitude of the liquid solder surface is higher than 10.2 μm.     

超声引起的SrAl_2O_4:Eu,Dy薄膜的应力发光

为了实现超声探伤和应力发光探伤二者结合,研究了SrAl_2O_4∶Eu,Dy(SAOED)/硅橡胶在超声振动下的应力发光性质。薄膜的微观结构表明,SrAl_2O_4∶Eu,Dy应力发光颗粒被高弹性的硅胶包裹。当超声波作用到薄膜表面时,超声振动可以促使应力发光颗粒周围的硅橡胶发生各种形变,从而使被包裹的应力发光颗粒发生有效形变,产生高效的应力发光。薄膜厚度以及超声频率对SAOED发光薄膜的应力发光有明显的影响。薄膜的超声应力发光强度随着薄膜厚度的增加先增大,当薄膜的厚度为1 mm时达到最大,之后随着薄膜厚度的增加而降低。薄膜的超声应力发光强度与超声频率大小成正比,即使最低频率仅为500 Hz时仍能检测到信号,说明SrAl_2O_4∶Eu,Dy/硅橡胶发光薄膜是一种很有应用前途的无损检测传感器。     

A novel ultrasonic micro-dissection technique for biomedicine

Molecular techniques are transforming our understanding of cellular function and disease. However, accurate molecular analysis methods will be limited if the input DNA, RNA, or protein is not derived from pure population of cells or is contaminated by the wrong cells. A novel Ultrasonic Vibration Micro-dissection (UVM) method was proposed to procure pure population of targeted cells from tissue sections for subsequent analysis. The principle of the ultrasonic vibration cutting is analyzed, and a novel micro-tool is designed. A multilayer piezoelectric actuator is used to actuate a sharp needle vibrating with high frequency and low amplitude (approx. 16-50 kHz, and 0-3 microm) to cut the tissue. Contrast experiment was done to test the feasibility of UVM method. Experimental results show that the embedded tissue can be quickly and precisely cut with the ultrasonic vibration micro-dissection method.     

Complex vibration ultrasonic welding systems with large area welding tips

Vibration and welding characteristics of complex vibration ultrasonic welding systems of 27 and 40 kHz were studied. Complex vibration systems, which have elliptical to circular or rectangular to square locus, are effective for ultrasonic welding of various specimens including the same and different metal specimens, and for direct welding of semiconductor tips and packaging of various electronic devices without solder. The complex vibration systems consist of a one-dimensional longitudinal-torsional vibration converter with slitted part, a stepped horn and a longitudinal vibration transducer as a driving source. The complex vibration welding tips of 27 and 40 kHz have enough area of 6-8 mm square for various welding specimens. Aluminum plate specimens of 0.3-1.0 mm thickness were successfully joined with weld strengths almost equal to aluminum specimen strength, and independent to the specimen direction. Required vibration amplitude of 40 kHz is smaller than that of 27 kHz.     

A theoretical model describing the transfer characteristics of a membrane hydrophone and validation

This paper presents a theoretical model describing the transfer characteristics of a bilaminar polyvinylidene fluoride membrane hydrophone. The model applied uses a matrix formulation for one-dimensional propagation in multi-layered media. Arbitrary interconnections of piezoelectric layers are possible. The electrical transfer characteristics of the hydrophone leg and cable are modelled as transmission lines using a two-port network approach. The model is validated against measured open-circuit sensitivities for a 25 microm film thickness 0.5 mm active element diameter bilaminar hydrophone (50 microm total film thickness). Good agreement between theory and experiment is demonstrated. A 9 microm film thickness 0.5 mm active element diameter bilaminar hydrophone (18 microm total film thickness) together with amplifier is also modelled. The voltage at the output stage of the amplifier is measured and, by knowledge of the transfer characteristics of the complete system, the pressure waveform at the hydrophone position is estimated and compared with the acoustic pressure waveform derived using a laser interferometer. Good agreement is obtained.     

钢-混凝土结构弱粘接界面缺陷的超声导波检测*

钢-混凝土结构是土木工程中的一种常用结构形式,钢与混凝土粘接处可能出现弱粘接甚至完全脱粘的缺陷,严重影响结构的安全性。该文提出利用空气耦合超声导波衰减的方法实现钢-混凝土结构粘接状态的非接触无损检测方法,分析不同厚度粘接界面对超声导波衰减的影响。基于全局矩阵技术对钢-混凝土结构求解理论频散方程和衰减曲线,得到界面层不同粘接条件下的理论参数及衰减特性。建立不同粘接条件的有限元模型,定量分析不同模态对粘接缺陷的检测敏感度。研究界面层厚度分别为1 mm和2 mm两种情况下S0能量的衰减情况。研究结果表明:S0模态可有效判断粘接结构的粘接状态,对于同一界面层厚度,随着界面粘接条件变弱,S0最大幅值与A0最大幅值比不断增大;不同厚度同一粘接条件下,2 mm相较于1 mm该值更大。该方法在钢-混凝土结构粘接界面缺陷的检测方面具有良好的应用价值和发展前景。     

基于二维声子晶体结构的大尺寸超声 塑料焊接系统*

在大尺寸的圆柱形超声塑料焊接系统的工具头上加工周期性槽,形成一种二维声子晶体结构。对基于二维声子晶体结构的圆柱形工具头进行了声子晶体的带隙分析,并对优化设计后的该超声塑料焊接系统进行数值模拟。探讨了开槽前后超声塑料焊接系统振型的变化以及工具头输出端面纵振动的位移分布。研究结果表明,二维声子晶体结构能有效地抑制横向振动,增强纵向振动,并均匀输出端面的振幅,可以实现对超声塑料焊接系统的优化设计。     

Ultrasonically assisted turning of aviation materials: simulations and experimental study

Ultrasonically assisted turning of modern aviation materials is conducted with ultrasonic vibration (frequency f approximately 20 kHz, amplitude a approximately 15 microm) superimposed on the cutting tool movement. An autoresonant control system is used to maintain the stable nonlinear resonant mode of vibration throughout the cutting process. Experimental comparison of roughness and roundness for workpieces machined conventionally and with the superimposed ultrasonic vibration, results of high-speed filming of the turning process and nanoindentation analyses of the microstructure of the machined material are presented. The suggested finite-element model provides numerical comparison between conventional and ultrasonic turning of Inconel 718 in terms of stress/strain state, cutting forces and contact conditions at the workpiece/tool interface.     

Sonophotocatalytic inactivation of E. coli using ZnO nanofluids and its mechanism

The present study evaluated inactivation efficiency of a sonophotocatalytic process using ZnO nanofluids including ultrasonic parameters such as power density, frequency and time. The result showed that inactivation efficiency was increased by 20% when ultrasonic irradiation was combined with photocatalytic process in the presence of natural light. Comparison of inactivation efficiency in photocatalytic, ultrasonic and sonocatalytic processes using Escherichia coli as a model bacteria identified that inactivation efficiencies are shown in the following order: ultrasonic irradiation < sonocatalysis < photocatalysis < sonophotocatalysis. Furthermore, inactivation mechanism of sonophotocatalysis was proposed. Studies of reactive oxygen species (ROS) and zinc ions (Zn²⁺) release evaluation revealed that ROS play a key role in bacterial inactivation rather than Zn²⁺. Permeability of outer membrane (OM) and inner membrane (IM) of E. coli bacterial cells were studied and exhibited that sonophotocatalysis increased the permeability of OM and IM significantly. The enhanced bacterial inactivation effect in sonophotocatalytic process contributed to acoustic cavitation, sonocatalysis of ZnO and sonoporation phenomenon.     

Double-structured ultrasonic high frequency reactor using an optimised slant bottom

The present work has been carried out in order to design a new type of ultrasonic reactor consisting of a double-structured tank. The inner working compartment is built with a slant bottom to allow a better ultrasonic transmission. This paper reports the effect of the inclination angle on acoustic efficiency for several different plates, e.g. two plates made of glass (2 mm and 3 mm thickness) and one made of PVC (3 mm thickness). The acoustic efficiency was determined as the ratio of the signal measured by a hydrophone in the presence of the plate to that signal in the absence of the plate. Having optimised the system, the ultrasonic powers in the inner and the outer compartments of the slant bottom reactor were determined by hydrogen peroxide dosimetry.     

共晶键合与减薄技术的压电能量采集器（英）

采用微硅 锆钛酸铅（Si-PZT）悬臂梁结构并在悬臂梁末端附加镍质量块,构成可以工作于低频环境（小于1 000 Hz）的微压电能量采集器,一种利用压电效应将环境振动能转换为电能的器件。利用金薄膜作为中间层的共晶键合技术和PZT研磨减薄技术制备了微压电悬臂梁结构,PZT减薄实验最好结果为减薄至8 m。镍质量块（2 mm2 mm0.6 mm）采用微电铸工艺制备。通过对硅片与块材PZT的共晶键合工艺与PZT减薄技术的研究,制备出总厚度约为71 m的Si-PZT悬臂梁结构,其中硅梁厚约为47 m,PZT梁厚约为24 m。制备的微压电振动能量采集器样品的测试结果表明:在谐振频率为950 Hz,1.0g加速度激励条件下,其交流输出峰值电压可达958 mV。     

Dislocation structure of kcl crystals grown with vibration of the melt

Summary The amplitude at all frequencies was 0.04 mm. Three crystals were grown at each frequency, with seeds of dislocation density D = 6 × 10⁴ cm^–2. Figure 1 shows the frequency dependence of the final D. Each point in Figs. 1 and 2 is the mean from 50 measurements (50 fields of view). At all frequencies except 180 Hz, D was 2–4 times less than that without vibration, while at 100 and 160 Hz it was less by nearly an order of magnitude.The effects of amplitude (0.02 to 0.2 mm) were examined at 100 Hz, the minimum D occurring at 0.1 mm (Fig. 2). At 0.04–0.08 mm, D was less by a factor 3–4 than for crystals grown without vibration, while at 0.1 mm it was less by an order of magnitude, being 2 × 10^–4 cm^–2. The size of the etch pits on crystals grown at amplitudes up to 0.1 mm did not differ from that for crystals grown at rest, but above 0.1 mm the size increased, by more than a factor 3 at 0.2 mm. Figure 3 illustrates these effects.Optimal vibration reduces D by improving the growth conditions (reduction of temperature gradients by mixing, more uniform impurity distribution).     

超声珩磨区实际气体的单空泡动力学分析

为进一步揭示功率超声振动的珩磨机理,以珩磨液为工作介质,研究了功率超声珩磨环境中实际气体的单空泡动力学特性。基于Rayleigh-Plesset方程,应用实际气体绝热方程和范德瓦尔斯方程对其进行了修正,建立了功率超声珩磨环境中实际气体的单空泡动力学方程以及实际气体单空泡共振频率方程。并运用4~5阶RungeKutta法模拟了不同超声条件(声压幅值、空泡初始半径、振动频率)对泡壁的运动以及运动速度的影响。结果表明:较高的声压幅值,空泡理论共振半径R'0与初始半径R0的比值为102数量级以及较低的超声频率有利于超声珩磨磨削区空化效应的发生。     

超声振动珩磨作用下空化泡动力学及影响参数

为了合理利用超声振动珩磨作用下的空化效应,以磨削区单个空化泡为研究对象,考虑珩磨头合成扰动速度和珩磨压力的作用建立了磨削区空化泡的动力学模型。数值模拟了空化泡初始半径,珩磨压力,液体静压力和超声声压幅值对磨削区空化效应的影响。研究表明考虑超声振动珩磨作用时,空化泡膨胀的幅值会受到抑制,其溃灭时间也会缩短,而且较容易出现稳态空化。珩磨压力和液体静压力对磨削区空化主要起抑制作用,超声波声压幅值在一定范围内能够促进磨削区空化效果的提升。本文的研究为进一步理解超声振动珩磨的空化机理提供了理论支持。     

A radial mode ultrasonic horn for the inactivation of Escherichia coli K12

Tuned cylindrical radial mode ultrasonic horns offer advantages over ultrasonic probes in the design of flow-through devices for bacterial inactivation. This study presents a comparison of the effectiveness of a radial horn and probe in the inactivation of Escherichia coli K12. The radial horn is designed using finite element analysis and the predicted modal parameters are validated using experimental modal analysis. A validated finite element model of the probe is also presented. Visual studies of the cavitation fields produced by the radial horn and probe are carried out using luminol and also backlighting to demonstrate the advantages of radial horns in producing a more focused cavitation field with widely dispersed streamers. Microbiological studies show that, for the same power density, better inactivation of E. coli K12 is achieved using the radial horn and, also, the radial horn offers greater achievable power density resulting in further improvements in bacterial inactivation. The radial horn is shown to be more effective than the probe device and offers opportunities to design in-line flow-through devices for processing applications.     

通孔阳极氧化铝膜的制备及其性能的研究

在15% H₂SO₄阳极氧化液中添加硝酸镨制备阳极氧化铝(AAO)膜以提高AAO膜的性能,采用化学腐蚀和微波处理相结合的方法,去除AAO膜的阻挡层,制备通孔的AAO膜。分别研究镨的添加量、氧化电压对AAO膜的厚度和硬度的影响及腐蚀时间、微波处理时间分别对AAO膜的阻挡层的影响,分别用能谱和扫描电镜等对AAO膜进行了表征。在15% H₂SO₄阳极氧化液中添加硝酸镨,制备出的AAO膜具有更大的厚度和硬度,当氧化电压为23 V时,在15% H₂SO₄+0.14 Pr g·L-1混合液中制备的AAO膜的厚度和硬度分别为162 μm和275.1 HV,与在阳极氧化液为15% H₂SO₄溶液中制备的AAO膜的厚度和硬度(150 μm和224.8 HV)相比,分别提高8.0%和22.4%。当氧化电压在19～23 V范围时,AAO膜的厚度随着氧化电压的增大而增加;AAO膜的硬度随着氧化电压的增大而减小。将AAO膜在35 ℃和5% H₃PO₄溶液中腐蚀13 min,再用超声波处理10 min,可得到通孔的AAO膜。腐蚀后AAO膜表面絮状物为Al₂O₃。     

0.90(Bi1/2Na1/2)TiO3–0.05(Bi1/2K1/2)TiO3– 0.05BaTiO3 transducer for ultrasonic wirebonding applications

Lead-free piezoelectric ceramic 0.90(Bi_1/2Na_1/2)TiO₃–0.05(Bi_1/2K_1/2)TiO₃–0.05BaTiO₃ (BNKBT-5) rings (OD=12.7 mm, ID=5.1 mm and 2.3-mm thick) were fabricated and characterized. Four ceramic rings were used as the driving element in an ultrasonic wirebonding transducer and the performance of the transducer was characterized. The lead-free transducer was found to have comparable voltage rise and fall times as a lead-based PZT transducer and has a relatively large vibration amplitude, thus showing that BNKBT-5 has the potential to be used in fabricating lead-free ultrasonic wirebonding transducers. PACS 77.22.Ej; 77.84.-s; 85.50.-n