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用未涂敷毛细管电泳分离DNA片段 总被引:1,自引:0,他引:1
用内壁未涂敷毛细管,以羟乙基纤维素的无胶筛分介质,在6min内分离了λDNA/EcoRI+HindⅢ片段,探讨了DNA片段在未涂敷毛细管中的分离机理,讨论了羟乙基纤维素和缓冲溶液浓度及电压对分离的影响,考察了DNA片段迁移时间的重现性。 相似文献
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以羟丙甲基纤维素和非交联聚丙烯酰胺溶液为筛分介质,将毛细管电泳-激光诱导荧光法用于DNA片段及基因扩增产物的分离检测。探讨了非胶筛分介质中高分子化合物的浓度、电解质的浓度、内插试剂用量等对DNA片段分析检测的影响;考察了DNA片段迁移时间和峰面积的重现性及DNA片段定量检测的关系,建立了一种快速、灵敏的DNA片段及基因扩增物分离检测方法。 相似文献
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毛细管电泳聚乙烯吡咯烷酮与羟乙基纤维素混配无胶筛分介质分离DN … 总被引:1,自引:0,他引:1
本文报道了毛细电泳聚乙烯吡咯烷酮与羟乙基纤维素混配无胶筛分介质分离较短的pGEM7Zf(+)-HaeⅢDBA片段(DNA长度为18~675bp)。研究表明,在1%的羟乙基纤维素无胶筛分介质中,加入2%的聚乙烯吡咯迷酮能显著提高DNA片段的分辨率和分离效率。在混配无胶筛分介质中,聚乙烯吡咯烷酮有两种作用,一是动态涂渍,降低毛细管内壁对DNA片段与DNA荧光插入试剂的吸附,改善分离效率;二是两种不同长 相似文献
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羟乙基纤维素及蔗糖无胶筛分毛细管电泳分离DNA片段 总被引:4,自引:0,他引:4
提出以羟乙基纤维素、蔗糖和硼酸形成“互穿网络”无胶筛分介质,显著地提高了分离效率,分离了φX174/HaeⅢDNA的所有11个DNA片段,探讨了“互穿网络”无胶筛分机理。将该方法应有和于决定鳝鱼性别基因PCR扩增产物的分离与鉴定,结果较好。 相似文献
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高效毛细管线性聚丙烯酰胺凝电泳柱的制备,柱性能表征及操作条件考察 总被引:8,自引:4,他引:4
发展一种简便快速的线性丙聚丙烯酰胺毛细管凝胶电泳柱的新型制备方法,用于分离PolydA(40-60)和双链DNA,柱效达6百万理论板/米。提出“筛分能力”作为CGE柱评价指标,对品迁移行为随操作条件的变化规律进行考察,为毛细管凝胶电泳的理论研究和实验条件优化奠定了基础。 相似文献
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玻璃微流控芯片十二烷基硫酸钠无胶筛分电泳测定免疫球蛋白片段分子量 总被引:2,自引:1,他引:2
通过实验优化了葡聚糖筛分介质和运行缓冲溶液的浓度,采用十二烷基硫酸钠(SDS)无胶筛分电泳分离体系(10%(w/v)葡聚糖,0.1%SDS,10%甘油,0.2mol/LTris-硼砂,pH8.3的缓冲液)在自制的玻璃微流控芯片上高效分离了BODIPY衍生的蛋白质分子量标准样品,连续6次电泳所得迁移时间的相对标准偏差均小于0.50%。以6种蛋白质分子量的对数对其迁移时间作图,线性回归良好(r=0.994)。采用该芯片电泳分析体系对免疫球蛋白G不同片段的分子量进行了测定,所得结果与实际基本相符。 相似文献
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用溶胶-凝胶法以磷钼酸(MPA)的镍盐溶液水解钛酸四丁酯制备了NiPMo/TiO2催化剂.使用ICP、 XRD、 TG-DTA、 IR、 TPD-MS和微反应技术研究了催化剂的化学组成、热稳定性、化学吸附性质和催化反应性能.杂多钼酸盐与TiO2通过O2-在TiO2表面发生了键合.在623 K下,杂多阴离子仍保持原有的Keggin结构.CO2在Lewis酸位Ni(Ⅱ)和Lewis碱位Ni-O-Mo的桥氧协同作用下生成CO2卧式吸附态Ni(Ⅱ)←O-(CO)←(O--Ni).丙烯有多种吸附态在催化剂上吸附.在563 K、 1 MPa和空速1500 h-1的反应条件下,丙烯的摩尔转化率为3.2%,产物MAA选择性为95%. 相似文献
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Toward new camptothecins. Part 6: Synthesis of crucial ketones and their use in Friedländer reaction
Laurent Gavara Thomas Boisse Jean-Pierre Hénichart Adam Daïch Philippe Gautret 《Tetrahedron》2010,66(38):7544-5571
In the context of the preparation of camptothecin and luotonin A analogs, the synthesis of some key keto-precursors and their use in Friedländer condensation are described. This paper also focuses on the stability of these keto intermediates and emphasizes the major differences between indolizinones and pyrroloquinazolinones series. Noteworthy is also the report of some original structures isolated as by-products of some experiments. 相似文献
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The Langevin paramagnetic theory can’t describe the relation between magnetization of ferrofluids and applied magnetic field. The structuralization of ferrofluids, which is considered the main influence factor of the magnetization, is regarded. The part of magnetization works is deposited when the structure is forming. This action influences the magnetization of ferrofluids directly or indirectly. On the base of the “compressing” model, the Langevin function that usually describes the magnetization of ferrofluid is modified, and a well-fitted curve is obtained. An equation of the relation between the equivalent volume fraction after being “compressed” and the intensity of magnetic field is discovered, which approximately describes the process of magnetization. The relation between the approximate initial susceptibility and the volume fraction can be obtained from modified formula. 相似文献
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Rebecca J. Burton Mandy L. CrowtherNeal J. Fazakerley Shaun M. FilleryBarry M. Hayter Jason G. KettleCaroline A. McMillan Paula PerkinsPeter Robins Peter M. SmithEmma J. Williams Gail L. Wrigley 《Tetrahedron letters》2013
The highly regioselective Buchwald–Hartwig amination at C-2 of the cheap and readily accessible reagent, 2,4-dichloropyridine with a range of anilines and heterocyclic amines is described. This new methodology is robust and provides a facile access to 4-chloro-N-phenylpyridin-2-amines on 0.25 mol scale. These intermediates undergo a further Buchwald–Hartwig amination at higher temperature to enable rapid exploration of the chemical space at C-4 and to provide a library of 2,4-bisaminopyridines. 相似文献
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KMnO4-mediated oxidative CN bond cleavage of tertiary amines producing secondary amine was introduced, which was trapped by electrophiles (acyl chloride and sulfonyl chloride) to form amides and sulfonamides. The reaction could take place at mild condition, tolerating a wide range of function groups and affording products in moderate to excellent yields. 相似文献
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N. A. Nedolya 《Chemistry of Heterocyclic Compounds》2008,44(10):1165-1219
The review contains a concise historical account and information on the most significant researches undertaken by the staff
at the A. E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch of the Russian Academy of Sciences on the Chemistry
of Heterocyclic Compounds.
Dedicated to Academician of the Russian Academy of Sciences B. A. Trofimov on his 70th jubilee.
Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 10, pp. 1443–1502, October, 2008. 相似文献
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Regiospecific and direct imidation of the methyl C(sp3)–H bond of thioanisoles is realized under mild and metal-free conditions with N-fluorobis(benzenesulfonyl)imide as an oxidant and nitrogen source. Proposed mechanism suggests that thionium ion intermediates and a Pummerer-type reaction are involved. The imidation has advantages such as high step-economy, excellent functionality tolerance, and regiospecificity, giving structurally diverse imidation products. 相似文献
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《Tetrahedron》2014,70(21):3377-3384
The Rh(II)-catalyzed reaction of 2-carbonyl-substituted 2H-azirines with ethyl 2-cyano-2-diazoacetate or 2-diazo-3,3,3-trifluoropropionate provides an easy access to 2H-1,3-oxazines and 1H-pyrrol-3(2H)-ones. These compounds can be selectively prepared from the same starting material using temperature as the only varied parameter. The 2-azabuta-1,3-diene intermediate, a common precursor for both heterocyclic products, isomerizes into 2H-1,3-oxazine under kinetic control, while 1H-pyrrol-3(2H)-one is the sole product of the reaction at elevated temperatures. According to DFT-calculations a one-atom oxazine ring contraction involving ring-opening to a 2-azabuta-1,3-diene intermediate, followed by a 1,5- and 1,2-prototropic shift leads to the consecutive formation of imidoylketene and azomethine ylide, which then further undergo cyclization to the pyrrole derivative. 相似文献
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Different approaches for the synthesis of 1-benzyloxypyrazin-2(1H)-one derivatives from simple amino acids have been investigated. A library of 33 precursors for the preparation of N-hydroxy pyrazinones was obtained in moderate to good yields. 相似文献