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反相高效液相色谱法同时测定茶叶中六种儿茶素组分的研究 总被引:9,自引:0,他引:9
采用反相高效液相色谱法同时测定茶叶中L (+) 表儿茶素(L EC),L (-) 表儿茶素没食子酸酯(L ECG),L (-) 表没食子儿茶素没食子酸酯(L EGCG),L (-) 表没食子儿茶素(L EGC),(+) 没食子儿茶素没食子酸酯(GCG),DL 儿茶素(DL C)六种儿茶素组分,以乙醇∶乙酸∶水=14.5∶1.0∶84.5(体积比)为流动相(1.0ml·min-1),C18柱,UV检测器(278nm)。六种儿茶素组分的相对标准偏差分别为0.67%,0.45%,0.24%,0.56%,3.11%,4 41%,回收率分别为95.2%,98.2%,101.3%,97.0%,98.3%,97.5%。该法采用乙醇、乙酸等无毒溶剂作流动相,回收率高,用于实际样品测定,结果满意。 相似文献
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反相高效液相色谱同时测定对乙酰氨基酚等5组分的含量 总被引:8,自引:0,他引:8
1 引 言在速效感冒液中同时存在着 5种成分 :对乙酰氨基酚、扑尔敏、咖啡因、愈创木酚甘油醚和对氨基酚。其中的对氨基酚是对乙酰氨基酚的降解产物 ,对人体有一定的副作用。因此 ,如何控制、降解对氨基酚的含量 ,一直是研究的重要课题之一 ,但尚未见到同时测定上述 5组分含量的报道。本文建立了运用反相高效液相色谱法同时测定感冒液中 5组分含量的方法。实验结果表明 :5组分在一定浓度范围内均呈良好的线性关系 ,回收率在 96 .31%~ 10 2 .2 3%之间。该方法操作简便、快速 ,结果准确。2 实验部分2 .1 仪器与试剂 Waters 5 10… 相似文献
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反相高效液相色谱法同时测定三七药材中4种皂苷的含量 总被引:9,自引:0,他引:9
建立了以0.02%磷酸-乙腈为流动相,梯度洗脱反相高效液相色谱同时测定中药材三七中三七皂苷R1、人参皂苷Rg1、Rb1和Rd 4种皂苷的新方法。R1、Rg1、Rb1和Rd 4种皂苷的加样回收率分别为89.54%、90.08%、82.82%与84.46%;线性范围分别为0.244-6.110、0.820-20.510、0.396-9.890与0.260-6.500μg。测定了不同规格、部位和来源的三七药材里的4种皂苷R1、Rg1、Rb1和Rd。方法准确可靠,结果稳定,重现性好,可用于三七及其制剂的质控。 相似文献
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反相高效液相色谱法同时测定食品中甜蜜素、糖精钠和苯甲酸钠 总被引:9,自引:0,他引:9
采用反相高效液相色谱法快速分离和测定食品中甜蜜素、糖精钠和苯甲酸钠,在ODS柱上,以30%甲醇和70%(含离子对试剂)水溶液为流动相进行分离;分别考察了流动相中不同的甲醇配比和不同浓度的离子对试剂对分离和保留行为的影响。检测波长为205nm。采用外标法定量。该方法精密度高、准确度好,样品处理简单,实用于常规质量监测。 相似文献
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反相高效液相色谱法同时测定水样中辛硫磷和氯菊酯 总被引:9,自引:0,他引:9
采用AgilentSBC8(5μm,4.6mmi.d.×150mm)色谱柱,以V(乙腈)∶V(水)=70∶30为流动相进行分离,二极管阵列检测器(PDA)在210nm波长处检测,建立了反相高效液相色谱法同时测定辛硫磷和氯菊酯的分析方法。辛硫磷和氯菊酯的线性范围分别为0.1~10μg mL和0.02~10μg mL,检出限分别为0 05和0.01μg mL,实际水样的加标回收率为90.3%和95.3%,相对标准偏差为1.3%和3.2%(n=7)。 相似文献
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鱼子酱中的合成色素反相高效液相色谱法同时测定 总被引:2,自引:0,他引:2
建立了反相高效液相色谱法同时测定鱼子酱中的柠檬黄、日落黄、偶氮玉红、苋菜红、胭脂红、赤藓红、红色2G和诱惑红8种合成色素的方法.样品中的合成色素经甲醇-4 mol/L尿素溶液(体积比1 ∶ 1)提取,固相萃取聚酰胺柱净化后,采用XDB-C18色谱柱(4.6 mm×250 mm×5 μm),以甲醇-乙腈(体积比1 ∶ 1)和0.01 mol/L乙酸钠溶液为流动相,在最佳梯度洗脱条件下,8种合成色素在17 min内实现分离.各色素在0.05 ~50 mg/L质量浓度范围内具有良好的线性关系.将该法用于实际样品的检测,8种合成色素的加标回收率为89% ~110%,相对标准偏差为1.4% ~4.7%.方法简单、高效,可用于鱼子酱中合成色素的日常监督检测. 相似文献
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采用更简便的流动相体系,建立了高效液相色谱法同时测定多维元素片中9种水溶性维生素的快速分析方法。以酸水解与离心的方法处理样品,用C8柱分离,流动相A为0.1%三氟乙酸的水溶液,B为甲醇,梯度洗脱,二极管阵列检测器(DAD)检测。28min内实现了9种水溶性维生素的同时分离测定。各维生素线性关系、精密度、回收率均良好。并使用美国国家标准技术研究院(NIST)的SRM3280多维元素片标准物质对方法进行了确认,运用此法测定了市售多维元素片中的水溶性维生素含量。该法可作为维生素片剂中水溶性维生素分离测定的质控方法。 相似文献
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Summary A procedure for the simultaneous quantitative determination of amiloride hydrochloride and hydrochlorothiazide by high-performance
liquid chromatography is proposed. A reversed-phase LiChrosorb C8 stationary phase is used. The eluent consisted of an acetonitrile/0.1M
phosphate buffer pH3 (15∶85) mixture, containing 50mM propylamine hydrochloride. In this system amiloride hydrochloride, a
basic drug, eluted with a acceptable asymmetry factor (Asf=2.1). A simple extraction procedure with methanol is used. Relative
standard deviations of 0.87% and 1.6% were obtained for amiloride hydrochloride and hydrochlorothiazide respectively. Chlorothiazide,
a thiazide diuretic, is a suitable internal standard. Furthermore the method is also specific for other thiazide diuretics,
potassium-sparing diuretics and loop diuretics and for the respective hydrolysis productes of both drugs. Analysis time is
reduced to a minimum; the chromatographic separation is complete within 6 minutes. 相似文献
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反相高效液相色谱法同时检测食物中毒样品中的敌鼠钠盐、杀鼠醚和杀鼠灵 总被引:1,自引:0,他引:1
建立了反相高效液相色谱-二极管阵列检测器同时快速检测食物中毒样品中的敌鼠钠盐、杀鼠醚和杀鼠灵的分析方法。固体样品用乙腈提取,液体样品用乙酸乙酯提取。以V(乙腈)∶V(0.010 mol/L乙酸铵)=57∶43(pH 4.50)为流动相,经C18色谱柱分离,二极管阵列检测器分别在波长286和306 nm处同时检测,在8 min内即可完成色谱分析。敌鼠钠盐、杀鼠醚和杀鼠灵在0.05~20.0μg/mL范围内与其色谱峰面积呈良好的线性关系,方法的检出限固体样品为0.015μg/g,液体样品为0.003μg/mL,样品加标平均回收率在85.0%~109.2%之间,相对标准偏差为1.0%~9.6%。 相似文献
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Simultaneous separation of nonionic surfactants and polyethylene glycols by reversed phase high performance liquid chromatography 总被引:2,自引:0,他引:2
Summary The simultaneous separation of polyethylene glycol and its derivatives such as the lauryl alcohol and lauric acid ethoxylate
oligomers was carried out by reversed phase high performance liquid chromatography. Branched fluorinated silica gel columns
combined with evaporative light scattering detection were used for the characterization of nonionic surfactants. Lauryl alcohol
ethoxylate oligomers were separated at 10°C with an isocratic eluent according to ethoxylate number and the retention time
of the oligomers decreases with increasing ethoxylate number. The Van’t Hoff plots of retention factor of lauryl alcohol ethoxylate
gave a complex cure, which is anomalous behavior for reversed phase high performance liquid chromatography. The anomalous
Van’t Hoff plots were explained by a partial conformational change from polar to less polar conformers with increasing temperature.
The most significant features for the analysis of the lauryl alcohol ethoxylate were the use of acetonitrile as mobile phase
and operating temperature. The polyethylene glycol was separated according to ethoxylate number and the retention time of
oligomers increased with increasing ethoxylate number. The Van’t Hoff plots of retention factor of polyethylene glycol had
negative slopes. It was presumed that the polar conformation of the ethylene oxide chain decreased with increasing temperature.
The lauryl alcohol ethoxylate and polyethylene glycol were separated simultaneously in gradient elution as a result of the
conformational change of the ethylene oxide chain. As a practical example, lauric acid ethoxylate simultaneously separated
into free polyethylene glycol, ethoxylate monolaurate and ethoxylate dilaurate in gradient elution. 相似文献
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高效液相色谱法同时测定厚朴温中胶囊中的7种有效成分 总被引:1,自引:0,他引:1
建立了同时测定厚朴温中胶囊中山姜素、甘草酸、和厚朴酚、小豆蔻明、木香烃内酯、去氢木香内酯及厚朴酚含量的反相高效液相色谱法。固定相为Scienhome C18柱(250 mm×4.6 mm,5 μm),流动相为甲醇-乙腈-0.06%磷酸溶液(体积比为38∶27∶35),流速为1.0 mL/min,柱温为30 ℃,检测波长为235 nm。在上述条件下,山姜素、甘草酸、和厚朴酚、小豆蔻明、木香烃内酯、去氢木香内酯及厚朴酚的质量浓度分别在0.885~17.7,107~2140,8.85~17.7,1.035~20.7,4.85~97,5.9~118和17.5~350 mg/L时与色谱峰面积之间的线性关系良好;回收率分别为96.9%~101.1%,96.0%~100.5%,100.3%~100.8%,97.7%~101.4%,100.4%~102.3%,96.0%~102.3%和96.2%~100.6%。该方法简便、快速、准确,可用于厚朴温中胶囊的质量控制。 相似文献
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I. G. Zenkevich S. V. Gushchina 《Russian Journal of Physical Chemistry A, Focus on Chemistry》2011,85(9):1641-1646
It is shown through reversed phase high performance liquid chromatography that a characteristic feature of such abundant natural
flavonoids as flavon-3-ols is an anomalously strong antibate dependence of their retention indices (RI) on the organic solvent concentration (C) in the eluent, dRI/dC < 0. In order to interpret this anomaly, the specific optical rotation values [α]D20 of natural (+)-(2R,3R)-dihydroquercetin in different solvents are compared, confirming the reverse formation of hydrated flavonoids in aqueous
solutions. 相似文献
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K. Jinno 《Journal of separation science》1982,5(7):364-367
The chromatographic performance of the deuterated solvents, CD3OD and D2O, has been investigated in reversed-phase micro high performance liquid chromatography. The chromatographic performance of CD3OD is only slightly superior to that of CH3OH. However, the performance of D2 is significantly superior to that of H2O, separation of aromatics being improved by about 30%. D2 is a particularly powerful solvent for the separation iof deuterated and non-deuterated compounds. 相似文献