Green supported Cu nanoparticles on modified Fe3O4 nanoparticles using thymbra spicata flower extract: Investigation of its antioxidant and the anti-human lung cancer properties

In recent days, the green synthesized nanomagnetic biocomposites have been evolved with tremendous potential as the future biological agents. This has encouraged us to design and synthesis of a novel Cu NPs supported Thyme flower extract modified magnetic nanomaterial (Fe₃O₄/Thyme-Cu). It was meticulously characterized using advanced analytical techniques like FT-IR, FESEM, TEM, EDX, VSM, XRD and ICP-OES. After the characterization, the synthesized Fe₃O₄/Thyme-Cu nanocomposite was engaged in biological assays like study of anti-oxidant properties by DPPH mediated free radical scavenging test using BHT as a reference molecule. Thereafter, on having a significant IC50 value in radical scavenging assay, we extended the bio-application of the desired nanocomposite in anticancer study of A549, Calu6 and H358 human lung cell lines in-vitro through MTT assay. They had very low cell viability and high anti-human lung cancer activities dose-dependently against A549, Calu6 and H358 cell lines without any cytotoxicity on the normal cell line (MRC-5). The IC50 of Fe₃O₄/Thyme-Cu nanocomposite was 124, 265, and 181 µg/mL against A549, Calu6 and H358 cell lines, respectively. Maybe significant anti-human lung cancer potentials of Fe₃O₄/Thyme-Cu nanocomposite against common lung cancer cell lines are related to their antioxidant activities. So, these results suggest that synthesized Fe₃O₄/Thyme-Cu nanocomposite as a chemotherapeutic nanomaterial have a suitable anticancer activity against lung cell lines.     

Immobilized Au nanoparticles on chitosan-biguanidine modified Fe3O4 nanoparticles and investigation of its anti-human lung cancer activity

In current nanoscience bioengineered magnetic nanoparticles (NPs) have come into prominence with considerable impact. These advanced functional materials find outstanding applications in chemical science in catalysis, environmental issues, sensing etc, as well as in biology as drug delivery agent, chemical therapeutics and others. We have been prompted to architect and synthesize a novel Au NP adorned over chitosan-biguanidine polyplex modified core–shell type magnetic nanocomposite (Fe₃O₄/CS-biguanidine/Au NPs). The bioshells facilitate to protect the core ferrite NPs as well as provides stability to the synthesized Au NPs by capping. The material was characterized using different analytical techniques like Fourier Transformed Infra-Red spectroscopy (FT-IR), Inductively Coupled Plasma-Optical Emission Microscopy (ICP-OES), Field Emission Scanning Electron Microscopy (FE-SEM), Energy Dispersive X-ray spectroscopy (EDX), Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and X-ray Diffraction (XRD) studies. We explored the biological application of the nanocomposite in determining cytotoxicity of three adenocarcinoma cell lines (PC-14, LC-2/ad, HLC-1) through the MTT assay. The material showed very good activity by exhibiting very low % cell viability over the cell lines dose-dependently. The IC50 of Fe₃O₄/CS-biguanidine/Au NPs were observed 503, 398 and 475 µg/mL respectively against the three cell lines. The best output was observed at a concentration of 1000 µg/mL of catalyst in terms of cytotoxicity and inhibition of lung cancer growth. The anti-cancer potential was found in close relation to their antioxidant potential.     

Facile preparation of Kaolin supported silver nanoparticles mediated by Thymbra spicata extract and investigation of the anti-human lung cancer properties

The present work demonstrates the synthesis of kaolin supported Ag nanoparticles (AgNPs@Kaolin) by using an aqueous extract of Thymbra spicata as a green reductant and capping agent. Physicochemical characteristics of the synthesized nanocomposite were studied by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), elemental mapping, X-ray diffraction (XRD) and inductively coupled plasma (ICP) techniques. The biogenic AgNPs are uniformly globular. Owing to the surface modification by the plant derived phytochemicals, the NPs are found to be distributed evenly on the kaolin surface. The AgNPs@Kaolin nanocomposite has been explored biologically in the anticancer and antioxidant assays. In the cellular and molecular part of the recent study, the treated cells with AgNPs@Kaolin nanocomposite were assessed by MTT assay for 48 h about the cytotoxicity and anti-human lung adenocarcinoma properties on normal (HUVEC) and lung adenocarcinoma cell lines i.e. lung well-differentiated bronchogenic adenocarcinoma (HLC-1), lung moderately differentiated adenocarcinoma (LC-2/ad), and lung poorly differentiated adenocarcinoma (PC-14). The viability of malignant lung cell line reduced dose-dependently in the presence of AgNPs@Kaolin nanocomposite. The IC50 of AgNPs@Kaolin nanocomposite were 509, 315, and 189 µg/mL against HLC-1, LC-2/ad, and PC-14 cell lines, respectively. In the antioxidant test, the IC50 of AgNPs@Kaolin nanocomposite and BHT against DPPH free radicals were 125 and 161 µg/mL, respectively. After the clinical study, AgNPs@Kaolin nanocomposite containing T. spicata leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human lung adenocarcinoma.     

Copper nanoparticles supported on polyethylene glycol-modified magnetic Fe3O4 nanoparticles: Its anti-human gastric cancer investigation

In that work, we have described the synthesis of novel Cu NPs decorated polyethylene glycol (PEG₂₀₀₀) coated magnetic nanoparticles (Fe₃O₄/PEG2000/Cu NPs) in an eco-friendly pathway applying Green Tea extract as reducing/stabilizing agent. The morphological and physicochemical features of the prepared nanocomposite were determined using several advanced techniques like ICP-OES, FE-SEM, EDX, atomic mapping, TEM, VSM, and XRD studies. In the antioxidant test, the IC50 of Fe₃O₄/PEG₂₀₀₀/Cu nanocomposite and BHT against DPPH free radicals were 198 and 85 µg/mL, respectively. In the cellular and molecular part of the recent study, the treated cells with Fe₃O₄/PEG₂₀₀₀/Cu nanocomposite were assessed by MTT assay for 48 h about the cytotoxicity and anti-human gastric cancer properties on normal (HUVEC) and gastric cancer cell lines i.e. NCI-N87 and MKN45. The IC50 of Fe₃O₄/PEG₂₀₀₀/Cu nanocomposite were 316 and 131 µg/mL against NCI-N87 and MKN45 cell lines, respectively. The viability of malignant gastric cell line reduced dose-dependently in the presence of Fe₃O₄/PEG₂₀₀₀/Cu nanocomposite. It seems that the anti-human gastric cancer effect of recent nanoparticles is due to their antioxidant effects.     

Green synthesis of Ag/Fe3O4 nanoparticles using Mentha extract: Preparation,characterization and investigation of its anti-human lung cancer application

In this study, silver nanoparticles (Ag NPs) were decorated on the surface of magnetic nanoparticles in an eco-friendly pathway applying Mentha extract as reducing/stabilizing agent. The morphological and physicochemical features of the prepared Ag/Fe₃O₄nanocomposite were determined using several advanced techniques. Hence, our protocol is green and advantageous in terms of- i) biochemical modified biocompatible nanocomposite; ii) nanomaterial providing high surface area and larger number reactive sites; iii) very simplistic synthetic procedure; vi) very low load of metal in the composite and v) high yield in short time. In the medicinal part, the anticancer properties of Ag/Fe₃O₄ nanocomposite against lung cancer cell lines were determined. The free radical for the antioxidant effects was DPPH. The IC₅₀ of Ag/Fe₃O₄ nanocomposite was 200 µg/ml in the antioxidant test. The IC₅₀ of the Ag/Fe₃O₄ nanocomposite were 183, 176, 169, and 125 µg/mL against lung cancer (NCI-H661, NCI-H1975, NCI-H1573, and NCI-H1563) cell lines, respectively. In addition, the current study offer that Ag/Fe₃O₄ nanocomposite could be a new potential adjuvant chemopreventive and chemotherapeutic agent against cytotoxic cells.     

Pectin mediated green synthesis of Fe3O4/Pectin nanoparticles under ultrasound condition as an anti-human colorectal carcinoma bionanocomposite

This work described the one-pot synthesis of apple pectin encapsulated Fe₃O₄ nanoparticles (Fe₃O₄/Pectin NPs) which is prepared by co-precipitation of Fe(II/(III) ions in alkaline solution mediated by pectin under ultrasound condition. This process led to formation of magnetic nanoparticles within the network of pectin. Physicochemical characterization of the as-synthesized Fe₃O₄/Pectin NPs was carried out through electron microscopy (SEM and TEM), energy dispersive X-ray spectroscopy (EDX), vibrating sample magnetometer (VSM) and X-ray diffraction (XRD). The in vitro cytotoxic and anti-colorectal cancer effects of biologically synthesized Fe₃O₄/Pectin NPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29 cancer cell lines were assessed. The anti-colorectal cancer properties of the Fe₃O₄/Pectin NPs could significantly remove Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29 cancer cell lines in a time and concentration-dependent manner by MTT assay. The IC₅₀ of the Fe₃O₄/Pectin NPs were 317, 337, 187, and 300 µg/mL against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29 cancer cell lines. The antioxidant activity of Fe₃O₄/Pectin NPs was determined by DPPH method. The Fe₃O₄/Pectin NPs showed the high antioxidant activity according to the IC₅₀ value. It seems that the anti-human colorectal cancer effect of recent nanoparticles is due to their antioxidant effects.     

Biosynthesis of Au NPs over modified Fe3O4 support using Rubia Tinctorum extract for the treatment of colorectal carcinoma in the in vitro condition: A pre-clinical trial study

In this study, an eco-friendly and low-cost procedure for the synthesis of Rubia Tinctorum plant extract modified magnetic nanocomposite (RT/Fe₃O₄) has been demonstrated. Au nanoparticles (Au NPs) were further decorated in situ over the designed RT/Fe₃O₄ nanocomposite exploiting the plant derived phytochemicals as bio-reductant and stabilizer. The resulting Au NPs@RT/Fe₃O₄ nanocomposite was characterized by various analytical methods like Field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDS), energy dispersive X-ray analysis (EDX) elemental mapping, transmission electron microscopy (TEM), vibrating-sample magnetometer (VSM), X-ray diffraction analysis (XRD), and inductively coupled plasma-atomic emission spectrometry (ICP-AES) analysis. The Au NPs@RT/Fe₃O₄ nanocomposite has been explored biologically in the anticancer and antioxidant assays. In the antioxidant test, the IC50 of Au NPs@RT/Fe₃O₄ nanocomposite and butylated hydroxytoluene (BHT) against 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals were 155 and 129 µg/ml, respectively. In the cellular and molecular part of the recent study, the treated cells with Au NPs@RT/Fe₃O₄ nanocomposite were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay for 48 h about the cytotoxicity and anti-human colon carcinoma properties on normal (HUVECs), colorectal adenocarcinoma (HT-29), colorectal carcinoma (HCT 116), ileocecal colorectal adenocarcinoma (HCT-8 [HRT-18]), and Burkitt's lymphoma (Ramos.2G6.4C10) cell lines. The viability of malignant colon cell line reduced dose-dependently in the presence of Au NPs@RT/Fe₃O₄ nanocomposite. The IC50 of Au NPs@RT/Fe₃O₄ nanocomposite were 250, 256, 212, and 197 µg/ml against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29 cell lines, respectively.     

Immobilized VOsalpr on modified Fe3O4 nanoparticles as a magnetically separable epoxidation catalyst

Fe₃O₄ nanoparticles were prepared and modified with chloropropyl trimethoxysilane (Cl-PTMS). The N,N′-bis(3-salicylidenaminopropyl)amine (salpr) Schiff base ligand was then immobilized on modified Fe₃O₄, which was followed by the addition of VOSO₄ in order to complex it with immobilized sa0lpr. The obtained nanocatalyst designated as VO(Salpr)/SCMNPs was characterized by FT–IR, XRD, SEM, TEM, and VSM techniques. It was found that VO(salpr)/SCMNPs successfully catalyze the epoxidation of allyl alcohols, such as limonene, 1-octene-3-ol, trans-2-hexene-1-ol and geraniol with 50 to 100% conversion and 62 to 100% selectivity with tert-butylhydroperoxide (TBHP). The study of this catalyst's stability and reusability revealed that VO(salpr)/SCMNPs behaves heterogeneously with no desorption during the course of the epoxidation reactions.     

Formulation and characterization of a novel anti-human endometrial cancer supplement by gold nanoparticles green-synthesized using Spinacia oleracea L. Leaf aqueous extract

Gold nanoparticles as one of the productions of the chemistry field have a special place in the cure of many diseases. Many experiences in medicinal researches have indicated that the plants enhance the anticancer effects of gold nanoparticles. According to the above contents, we investigated the capacities of Spinacia oleracea L. leaf aqueous extract green-mediated gold nanoparticles (AuNPs) as a modern chemotherapeutic material in treating endometrial cancer. The physicochemical characterization tests including UV–Visible Spectroscopy (UV–Vis), Field Emission Scanning Electron Microscopy (FE‐SEM), Fourier Transformed Infrared Spectroscopy (FT‐IR), and Transmission Electron Microscopy (TEM) were used for investigating the physicochemical properties of AuNPs. To survey the antioxidant potentials of AuNPs, one of the common antioxidant techniques i.e., DPPH was used. Determination of anti-endometrial cancer effects of AuNPs was carried out by the MTT assay and against Ishikawa, KLE, HEC-1-A, and HEC-1-B cell lines. The physicochemical characterization analyses revealed that the AuNPs had been formulated as the best possible. The results of the DPPH test confirmed excellent antioxidant properties of AuNPs in comparison to the butylated hydroxytoluene. The AuNPs IC50 was 194 µg/mL in the antioxidant test. The results of the MTT assay confirmed removing Ishikawa, KLE, HEC-1-A, and HEC-1-B cell lines after treating with low concentrations of AuNPs. The IC50 of the AuNPs was 341, 335, 316, and 325 µg/mL against Ishikawa, KLE, HEC-1-A, and HEC-1-B cell lines, respectively. The best finding of anti-endometrial cancer potentials was determined in the HEC-1-A cell line. According to the above results, significant anti-endometrial cancer effects of Au nanoparticles green-mediated by S. oleracea leaf extract are confirmed. It is offered that the studies of the clinical trial are performed for approving the above findings in humans.     

Signal amplification for DNA detection based on the HRP-functionalized Fe3O4 nanoparticles

An electrochemical approach for the sensitive detection of sequence-specific DNA has been developed. Horseradish peroxidase (HRP) assembled on the Fe₃O₄ nanoparticles (NPs) were utilized as signal amplification sources. High-content HRP was adsorbed on the Fe₃O₄ NPs via layer-by-layer (LbL) technique to prepare HRP-functionalized Fe₃O₄ NPs. Signal probe and diluting probe were then immobilized on the HRP-functionalized Fe₃O₄ NPs through the bridge of Au NPs. Thereafter, the resulting DNA-Au-HRP-Fe₃O₄ (DAHF) bioconjugates were successfully anchored to the gold nanofilm (GNF) modified electrode surface for the construction of sandwich-type electrochemical DNA biosensor. The electrochemical behaviors of the prepared biosensor had been investigated by the cyclic voltammetry (CV), chronoamperometry (i-t), and electrochemical impedance spectroscopy (EIS). Under optimal conditions, the proposed strategy could detect the target DNA down to the level of 0.7 fmol with a dynamic range spanning 4 orders of magnitude and exhibited excellent discrimination to two-base mismatched DNA and non-complementary DNA sequences.     

Fe3O4 nanoparticles decorated smectite nanocomposite: Characterization,photocatalytic and electrocatalytic activities

Magnetite nanoparticles-decorated smectite nanocomposite was prepared by precipitation method and the obtained nanocomposite was used as both nanophotocatalyst for removal of rhodamine B from wastewater under UVA irradiation and electrocatalyst for the electrooxidation of chlorite ion on the carbon paste electrode. The raw smectite, bare magnetite and the synthesized nanocomposite catalyst were comparatively characterized by using XRD, FTIR, SEM, EDX, XPS, VSM, TG/DTG, DTA, DSC, electrophoretic mobility and BET techniques in detail. The XRD, SEM, electrophoretic mobility and VSM results indicated that the magnetite nanoparticles were uniformly distributed on the surface of smectite with a diameter of about 7 nm by electrostatic interactions and the prepared nanocomposite displayed well superparamagnetic behaviour with strong saturation magnetization at room temperature. The XPS, EDX, FTIR and thermal analysis data of synthesized nanocomposite further confirmed that the magnetite nanoparticles were successfully decorated on the smectite without formation of another ferric phase species. Furthermore, the surface area of magnetite smectite nanocomposite is higher than that of raw smectite, which is related to the magnetite nanoparticles decorated onto the smectite. The catalytic activities of all samples were comparatively investigated by using the degradation of aqueous rhodamine B solutions as a model pollutant in the heterogeneous photo-like-Fenton process which is well defined by the pseudo-first-order equation in kinetics. The resultant magnetite-smectite nanocomposite showed excellent magnetic separability and much better photocatalytic activity in a short period compared to the bare magnetite and smectite. The synergetic effect between magnetite and smectite showed high activity not only in photodegradation but also in electrocatalytic applications. Because the developed nanocomposite material exhibited enhanced catalytic activity towards to chlorite ion oxidation it was applied for the voltammetric quantification of chlorite ion in aqueous medium. After the optimization of the measurement parameters, the limit of detection and quantification of the method were calculated as 3.0 and 10.0 μM, respectively.     

White tea extract modified green synthesis of magnetite supported Ag nanoparticles: Evaluation of its catalytic activity,antioxidant and anti-colon cancer effects

In this study, an eco-friendly and low-cost procedure for the synthesis of White tea plant extract modified magnetic nanocomposite (Fe₃O₄@W.tea) has been demonstrated. Ag nanoparticles (Ag NPs) were further decorated in situ over the designed Fe₃O₄@W.tea nanocomposite exploiting the plant derived phytochemicals as bio-reductant and stabilizer. The resulting Fe₃O₄@W.tea/Ag nanocomposite was characterized by various analytical methods like Fourier Transformed Infra Red (FT-IR) spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), energy dispersive X-ray analysis (EDX) elemental mapping, transmission electron microscopy (TEM), vibrating-sample magnetometer (VSM), X-ray diffraction analysis (XRD), and inductively coupled plasma-atomic emission spectrometry (ICP-AES) analysis. The as-synthesized bio-nanomaterial was used as an excellent heterogeneous and magnetically retrievable catalyst in the three-component condensation of 4-hydroxycoumarin, malononitrile and various aldehydes in refluxing aqueous media. A broad range of aromatic aldehydes underwent the reaction to produce diverse pyrano[3,2-c]chromene derivatives in very good yields irrespective of the nature of bearing functional groups or their respective geometrical positions. Due to superparamagnetic character, the material was easily magnetically decanted out and recycled for 8 successive times with preservation of its catalytic activity. After the chemical applications we also explored the material biologically in the resistance of human colon cancer and thereby studied the cytotoxicity over two standard cell lines, HT-29 and Caco-2. The conventional MTT assay was carried out over them which revealed an increase in % cell viability dose dependently. The IC₅₀ values observed in the two cell lines were 384.2 μg/ml and 254.6 μg/ml respectively. In addition, DPPH radical scavenging test was performed for studying anti-oxidant activity. The results validate the administration of Fe₃O₄@W.tea/Agnanocomposite as a competent colon protective drug in the clinical trial studies over human.     

Study on the adsorption of DNA on Fe3O4 nanoparticles and on ionic liquid-modified Fe3O4 nanoparticles

We have investigated the adsorption of herring sperm DNA on Fe₃O₄ magnetic nanoparticles (NPs) before and after modification with the ionic liquid 1-hexyl-3-methylimidazolium bromide. Experiments were performed in a batch mode, and the effects of DNA concentration, pH of the sample solution, ionic strength, temperature, and contact time between reagents were optimized. An evaluation of the adsorption isotherm revealed that the Langmuir model better fits the equilibrium data than the Freundlich model. The maximum adsorption capacities of the unmodified and modified NPs, respectively, were found to be 11.8 and 19.8 mg DNA per gram of adsorbent. The adsorption of DNA onto the modified NPs was endothermic, while it was exothermic in the case of the unmodified NPs. The DNA can be desorbed from the modified surfaces of the NPs by using EDTA as the eluent. The NPs were able to adsorb about 90?±?1.5 % of DNA after being recycled for three times. The method is simple, fast, robust, and does not require organic solvents or sophisticated equipment.

Water-dispersible Fe3O4 nanoparticles stabilized with a biodegradable amphiphilic copolymer

In this work, we report a green synthetic method using water-dispersible magnetite nanoparticles containing oleic acid and poly(2-ethyl-2-oxazoline)-poly(ɛ-caprolactone) diblock copolymer as the magnetite nanoparticle dispersants. The Fe₃O₄ nanoparticles were prepared by co-precipitation and had a bilayer surface with a hydrophobic inner poly(ɛ-caprolactone) (PCL) layer and hydrophilic corona poly(2-ethyl-2-oxazoline) (POX) blocks. Also, the role of the ultrasonicating treatment's duration on the percent of magnetite in the complex and on its magnetic properties was investigated. Transmission electron microscopy (TEM) showed the average particle size to be about 10–20 nm in diameter for nanoparticles.     

Improved magnetic properties of self-composite SrFe12O19 powder prepared by Fe3O4 nanoparticles

Sintered polycrystalline strontium hexaferrite, which is one of the most widely used permanent magnetic materials, has been applied in many areas such as electrical and mechanical transductor devices. Improving the coercivity (H_cJ) of M-type strontium hexaferrite with a nominal composition SrFe₁₂O₁₉ (SrM) plays an essential role to adapt to many modern applications, but is limited by the process and purity of raw materials. In this work, we prepared SrM powders with different iron resources, sintering temperatures, and Fe/Sr atom ratios. It was found that using Fe₃O₄ nanoparticles with a Fe/Sr atom ratio of 12 at 1100 °C performed the self-composites with 70% nano-size and 30% micro-size, which had the best performance. The coercivity reaches 348.9 kA/m, which is improved compared to pure SrM obtained by other iron resources in the solid-state method. This process shows a unique formation mechanism and the resulting self-composite microstructure eventually causes enhancement in the properties, which provides a new way for high-performance hexaferrites.     

Bio-supported of Cu nanoparticles on the surface of Fe3O4 magnetic nanoparticles mediated by Hibiscus sabdariffa extract: Evaluation of its catalytic activity for synthesis of pyrano[3,2-c]chromenes and study of its anti-colon cancer properties

In this work, we have described the biogenic synthesized copper nanoparticles being supported over plant phytochemicals modified magnetic Fe₃O₄ nanoparticles. Hibiscus sabdariffa extract was used as a green reducing agent and an excellent stabilizer of the synthesized NPs. The biomolecules are adorned as a protective shell over the core ferrite NPs. Physicochemical characterization of the as-synthesized Cu-Hibiscus@Fe₃O₄ nanocomposite was carried out through Fourier transformed infrared spectroscopy (FT-IR), electron microscopy (SEM and TEM), energy dispersive X-ray spectroscopy (EDX), elemental mapping (WDX), vibrating sample magnetometer (VSM), X-ray diffraction (XRD) and inductively coupled plasma-optical emission spectroscopy (ICP-OES). The as-synthesized bio-nanomaterial was used as an excellent heterogeneous and magnetically retrievable catalyst in the three-component condensation of 4-hydroxycoumarin, malononitrile and various aldehydes in refluxing aqueous media. A broad range of aromatic aldehydes underwent the reaction to produce diverse pyrano[3,2-c]chromene derivatives in very good yields irrespective of the nature of bearing functional groups or their respective geometrical positions. Due to superparamagentic character, the material was easily magnetically decanted out and recycled for 8 successive times with preservation of its catalytic activity. After the chemical applications we also explored the material biologically in the resistance of human colon cancer and thereby studied the cytotoxicity over two standard cell lines, HT-29 and Caco-2. The conventional MTT assay was carried out over them which revealed an increase in % cell viability dose dependantly. In addition, DPPH radical scavenging test was performed for studying anti-oxidant activity, using BHT as the positive control. The IC50 values observed in the two cell lines were 490.12 μg/ml and 412.23 μg/ml respectively. The results validate the administration of Cu-Hibiscus@Fe₃O₄ as a competent colon protective drug in the clinical trial studies over human.     

α-Glucosidase immobilization on functionalized Fe3O4 magnetic nanoparticles for screening of enzyme inhibitors

α-Glucosidase was stereoscopically immobilized on the surface of Fe₃O₄ magnetic nanoparticles, which was modified with APTES, using GA as a cross-linker. This established method had a broad application prospect for screening of enzyme inhibitors.     

Decorated CuO nanoparticles over chitosan-functionalized magnetic nanoparticles: Investigation of its anti-colon carcinoma and anti-gastric cancer effects

In this study, a green protocol for supporting CuO nanoparticles over chitosan-modified amino-magnetic nanoparticles is described. The physicochemical and morphological properties of the desired nanocomposite assessed by various techniques like ICP, FT-IR, FE-SEM, EDX, TEM, XRD and VSM. In the oncological part of the recent study, the Cu(NO₃)₂, Fe₃O₄, and Fe₃O₄-NH₂@CS/CuO nanocomposite cell viability was very low against human gastric cancer cell lines i.e. MKN45, AGS, and KATO III and human colorectal carcinoma cell lines i.e. HT-29, HCT 116, HCT-8 [HRT-18], and Ramos.2G6.4C10. The IC50 of Fe₃O₄-NH₂@CS/CuO nanocomposite against MKN45, AGS, KATO III, HT-29, HCT 116, HCT-8 [HRT-18], and Ramos.2G6.4C10 cell lines were 517, 525, 544, 282, 214, 420, and 477 µg/mL, respectively. Thereby, the best anti-gastro-duodenal cancers findings of our Fe₃O₄-NH₂@CS/CuO nanocomposite was seen in the HCT 116 cell line case.     

Detection and distinguishability of leukemia cancer cells based on Au nanoparticles modified electrodes

The Au nanoparticles (Au NPs) modified interface has been fabricated by multi-potential step electrodeposition in this study. Based on the nano-Au interface, we have proposed an electrochemical approach to detect the cancer cell numbers sensitively with a detection limit of about 500 cells. More interestingly, the drug sensitive leukemia K562 cells and drug resistant leukemia K562/adriamycin could be electrochemically distinguished on the interface by the oxidation potential, which did not show any evident differences on the bare electrode. These results indicate the promising application of this nano-interface for constructing the unlabeled potential-discriminative cell biosensors.     

正癸酸修饰磁性纳米Fe3O4对溶菌酶的吸附研究

以沉淀法制备了正癸酸修饰磁性纳米Fe3O4,采用XRD、TEM和FT-IR对修饰前后的磁性纳米粒子的形态、结构进行了表征。将修饰后的磁性纳米粒子用于对溶菌酶蛋白进行吸附分离,研究了溶液的pH、温度、时间、溶菌酶初始浓度、离子强度等因素对吸附过程的影响。结果表明:pH=10.7,吸附温度为25℃,吸附时间为2.0 h,溶菌酶初始浓度为0.30 mg·mL-1,最大吸附容量为35.0 mg·g-1。修饰后的磁性纳米粒子用于从鸡蛋清中提取溶菌酶,纯化倍数为30.9,酶活力收得率为73.0%。