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1.
Gold nanoparticles (Au-NPs) with negative charges aggregate in the presence of Arg(6) due to electrostatic interactions resulting in the red-shift of the plasmon absorption. But, after incubation of Arg(6) with trypsin the aggregation of Au-NPs can be prohibited. Accordingly, a newly designed-Au-NPs based colorimetric assay method for trypsin activity is established. Trypsin with a concentration as low as 1.6 ng mL(-1) can be assayed with this new colorimetric assay. This colorimetric label-free assay for trypsin can be performed in aqueous solution and both Au-NPs and Arg(6) are easily accessible. Thus, this assay method is useful for screening inhibitors of trypsin. 相似文献
2.
A new convenient colorimetric sensor for fructose based on anti-aggregation of citrate-capped gold nanoparticles(Au NPs) is presented. 4-Mercaptophenylboronic acid(MPBA) induces the aggregation of Au NPs, leading to a color change from red to blue. Fructose as a potent competitor has strong affinity for MPBA and a borate ester is formed between MPBA and fructose. There is an obvious color change from blue to red with increasing the concentration of fructose. The anti-aggregation effect of fructose on Au NPs was seen by the naked eye and monitored by UV–vis spectra. Our results showed that the absorbance ratio(A_(519)/A_(640)) was linear with fructose concentration in the range of 0.032–0.96 μmol/L(R~2= 0.996), with a low detection limit of 0.01 μmol/L(S/N = 3). Notably, a highly selective recognition of fructose was shown against other monosaccharide and disaccharide(glucose, mannose, galactose,lactose and saccharose). With anti-aggregation assays higher selectivity is achievable. The results of this work provide a rapid method for evaluating the quantitative analysis of fructose in human plasma at physiologically meaningful concentrations and at neutral pH. The proposed procedure can be used as an efficient method for the precise and accurate determination of fructose. 相似文献
3.
A new luminescent europium probe is presented for the determination of phosphate (P) in microtiter plate format. The assay is based on the quenching of the luminescence of the europium-tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 μmol/L. The probe is excited at 400 nm and displays a large Stokes’ shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7 and is therefore suitable for phosphate determination in biological and biochemical systems. The linear range of the calibration plot is from 5 × 10−6 mol/L to 7.5 × 10−4 mol/L of phosphate, and the limit of detection is 3 μmol/L. 相似文献
4.
A novel biosensing principle is presented, based on the potentiometric monitoring of an indicator ion such as Ca2+, whose zero-current flux through chemically modified nanochannels is altered by biorecognition events. 相似文献
5.
《印度化学会志》2021,98(2):100029
Alkaline phosphatase (ALP) is an important biomarker in clinical diagnostics, and the abnormal level of ALP enzyme in serum is closely related to various diseases such as bone metastases, bone or liver cancer, and extrahepatic biliary obstruction. Recognizing the location and expression level of ALP in live cells has a substantial importance in early-stage cancer diagnosis, as well as an important parameter for studying the recovery of the patients after liver transplantation. With the advent of the newer and advanced fluorescence imaging techniques, small-molecule fluorescent probes have become a very powerful tool for mapping the subtle changes in the enzyme expression level in living cells and tissues in real-time. In this account, we provide an overview of recent advances in small-molecule ALP fluorescent probes, mainly during the last few years, including the design strategies and applications for biological applications. 相似文献
6.
In this study, a new type of localized surface plasmon resonance (LSPR) sensing substrate for phosphopeptides was explored.
It has been known that LSPR response for target species is larger in the near-infrared region (NIR) than in the visible region
of the electromagnetic spectrum. Several types of noble metal nanoparticles (NPs) with NIR absorption capacities have been
previously demonstrated as effective LSPR-sensing nanoprobes. Herein, we demonstrate a straightforward approach with improved
sensitivity by simply using layer-by-layer (LBL) spherical Au NPs self-assembled on glass slides as the LSPR-sensing substrates
that are responsive in the NIR region of the electromagnetic spectrum. The modified glass slide acquired an LSPR absorption
band in the NIR, which resulted from the dipole–dipole interactions between Au NPs. To enable the chip to sense phosphopeptides,
the surface of the glass chip was spin-coated with thin titania film (TiO2-Glass@Au NPs). Absorption spectrophotometry was employed as a detection tool. Tryptic digest of α-casein was used as a model
sample. The feasibility of using the new LSPR approach for detecting a potential risk factor leading to cancers (i.e., phosphorylated
fibrinopeptide A) directly from human serum samples was demonstrated. Matrix-assisted laser desorption/ionization mass spectrometry
(MALDI-MS) was used to confirm the results. 相似文献
7.
Nitroexplosives, such as 2,4,6-trinitrotoluene (TNT) which is a leading example of nitroaromatic explosives, are causing wide concern. Motivated by the urgent demand for trace analysis of explosives, novel surface-enhanced Raman spectroscopy substrates based upon highly ordered Au nanoparticles have been fabricated by a simple droplet evaporation method. It is noteworthy that an ethylhexadecyldimethyl ammonium bromide bilayer surrounding each individual nanoparticle not only is responsible for these periodic gap structures, but also tends to promote the adsorption of TNT on the composite NPs, thus resulting in a considerable increase of Raman signal. These desirable features endow the resulting SERS substrates with excellent enhancement ability and allow for a label-free detection of common plastic explosive materials even with a concentration as low as 10−9 M. 相似文献
8.
E. Babu S. Singaravadivel P. Manojkumar S. Krishnasamy G. Gnana kumar S. Rajagopal 《Analytical and bioanalytical chemistry》2013,405(21):6891-6895
We report a simple, cost-effective, and label-free detection method, consisting of a platelet-derived growth factor (PDGF) binding aptamer and hydrophobic Ru(II) complex as a sensor system for PDGF. The binding of PDGF with the aptamer results in the weakening of the aptamer–Ru(II) complex, monitored by luminescence signal. A substantial enhancement in the luminescence intensity of Ru(II) complex is observed in the presence of aptamer due to the hydrophobic interaction. Upon addition of PDGF, the luminescence intensity is decreased, due to the stronger interaction between the aptamer and PDGF resulting in the displacement of Ru(II) complex to the aqueous solution. Our assay can detect a target specifically in a complex medium such as the mixture of proteins, at a concentration of 0.8 pM. Figure
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9.
Carbon nanodots as fluorescence probes for rapid, sensitive, and label-free detection of Hg2+ and biothiols in complex matrices 总被引:1,自引:0,他引:1
In this work, unmodified carbon nanodots are demonstrated as novel and environmentally-friendly fluorescence probes for the sensing of Hg(2+) and biothiols with high sensitivity and selectivity. 相似文献
10.
Optical devices were fabricated by sandwiching a “monolithic” poly(N-isopropylacrylamide-co-N-(3-aminopropyl) methacrylamide hydrochloride) (pNIPAm-co-APMAH) microgel layer between two semitransparent Au layers. These devices, referred to as etalons, exhibit characteristic multipeak reflectance spectra, and the position of the peaks in the spectra primarily depends on the distance between the Au surfaces mediated by the microgel layer thickness. Here, we show that the positively charged microgel layer can collapse in the presence of negatively charged single stranded DNA (ssDNA) due to ssDNA induced microgel crosslinking. The collapse results in a change in the etalon's optical properties, which can be used to detect target DNA in a complex mixture. 相似文献
11.
Use of cellulose derivatives on gold surfaces for reduced nonspecific adsorption of immunoglobulin G
Sondre Volden Kaizheng Zhu Bo Nystrm Wilhelm R. Glomm 《Colloids and surfaces. B, Biointerfaces》2009,72(2):266-271
This study addresses the design of protein-repellent gold surfaces using hydroxyethyl- and ethyl(hydroxyethyl) cellulose (HEC and EHEC) and hydrophobically modified analogues of these polymers (HM-HEC and HM-EHEC). Adsorption behavior of the protein immunoglobulin G (IgG) onto pure gold and gold surfaces coated with cellulose polymers was investigated and described by quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM) and contact angle measurements (CAM). Surfaces coated with the hydrophobically modified cellulose derivatives were found to significantly outperform a reference poly(ethylene glycol) (PEG) coating, which in turn prevented 90% of non-specific protein adsorption as compared to adsorption onto pure gold. HEC and EHEC prevented around 30% and 60% of the IgG adsorption observed on pure gold, while HM-HEC and HM-EHEC were both found to completely hinder biofouling when deposited on the gold substrates. Adsorption behavior of IgG has been discussed in terms of polymer surface coverage and roughness of the applied surfaces, together with hydrophobic interactions between protein and gold, and also polymer-protein interactions. 相似文献
12.
A label-free electrochemical method is developed for simple and convenient quantification of gene-specific DNA hypermethylation in a DNA sequence without PCR amplification, bisulfite conversion or labeling processes. 相似文献
13.
Joe Sarobe Iris Miraballes Jos Antonio Molina Jacqueline Forcada Roque Hidalgo-lvarez 《先进技术聚合物》1996,7(9):749-753
Monodisperse core–shell stable latexes with reactive methylchloride surface functionalities were prepared at two different reaction temperatures. The reaction temperature played an important role in the amount of reactive functional groups. The covalent coupling had an efficiency of more than 50%. Antibodies covalently bound to functionalized polystyrene beads were used to detect corresponding antigens by nephelometry. 相似文献
14.
Lin R Yip JH Zhang K Koh LL Wong KY Ho KP 《Journal of the American Chemical Society》2004,126(48):15852-15869
A luminescent molecular rectangle [Au(4)(micro-PAnP)(2)(micro-bipy)(2)](OTf)(4) (1.(OTf)(4)) (PAnP = 9,10-bis(diphenylphosphino)anthracene, bipy = 4,4'-bipyridine, X = NO(3)(-) or OTf(-)), synthesized from the self-assembly of the molecular "clip" Au(2)(micro-PAnP)(OTf)(2) and bipy, shows a large rectangular cavity of 7.921(3) x 16.76(3) A. The electronic absorption and emission spectroscopy, and electrochemistry of the metallacyclophane, have been studied. The 1(4+) ions are self-assembled into 2D mosaic in the solid state via complementary edge-to-face interactions between the Ph groups. (1)H NMR titrations ratify the 1:1 complexation between 1(4+) and various aromatic molecules. Comparing the structures of the inclusion complexes indicates an induced-fit mechanism operating in the binding. The emission of 1(4+) is quenched upon the guest binding. The binding constants are determined by both (1)H NMR and fluorescence titrations. Solvophobic and ion-dipole effects are shown to be important in stabilizing the inclusion complexes. 相似文献
15.
We developed a novel strategy to prepare functionalized fluorescent gold nanodots (AuNDs) based on a ligand exchange reaction and demonstrated that glutathione modified AuNDs can be utilized for highly sensitive and selective Pb(2+) sensing in aqueous solution. 相似文献
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18.
Herein, we reported a cationic conjugated polymers-based new biosensor with label-free and fluorescence turn-on strategy by virtue of targets regulated aggregation and quenching ability of perylene diimide derivatives. 相似文献
19.
Carbon nanodots (C-Dots) have attracted much attention in recent years due to their low cost, ready scalability, excellent chemical stability, biocompatibility and multicolor luminescence. Here, we report a facile strategy for producing highly luminescent, surface-different nitrogen-doped carbon dots (C-Dots) by using different ionic liquids (ILs). Intriguingly, the surface-different C-Dots show different selectivity for Cu2+ and Fe3+. To the best of our knowledge, this is the first example which shows that ILs are excellent precursors for producing luminescent nanomaterial used for detection of different metal ions. The resultant nitrogen-doped C-Dots are highly photoluminescent and can be used for multicolor bioimaging. Most notable, by taking different ILs as precursors, we obtain surface-different C-Dots, which can be directly used for selective detection of Cu2+ and Fe3+ without any modification. These C-Dots based sensors exhibit high sensitivity and selectivity and the sensing process can be easily accomplished with one-step rapid operation. More importantly, compared with other method using QDs, organic dyes and organic solvent, this strategy is much more eco-friendly. This work may offer a new approach for developing low cost and sensitive C-Dots-based sensors for biological and environmental applications. 相似文献