共查询到18条相似文献，搜索用时 46 毫秒
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通过席夫碱反应将2-氨基4-甲基吡啶与4-(二乙氨基)水杨醛结合,设计并合成出一种新型的荧光探针L,该探针能特异性识别Zn2+。通过质谱、1H NMR以及13C NMR表征其结构,并利用荧光光谱、紫外-可见吸收光谱研究了探针L在CH3OH-H2O(V:V=8:2,Tris-HCl缓冲液,pH=7.4)中对各种离子的选择识别能力。实验结果显示,向探针L中加入Zn2+之后,溶液从无荧光变成蓝色荧光,且457 nm处出现一发射峰。Job’s plot工作曲线结果和密度泛函理论(DFT)计算表明探针L与Zn2+结合计量比为1:1。荧光滴定结果表明探针L对Zn2+的检测极限可低至2.7×10-8 mol·L-1,结合常数为1.32×104 L·mol-1,pH应用范围4.0～10.0。对真实水样中的Zn2+进行检测,平均回收率大于98%,RSD小于1.61%,表明探针L能够检测真实水样中的Zn2+。 相似文献
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以乙二胺与水杨醛及其衍生物为原料,构建了4种席夫碱Zn2+荧光探针,其结构均由1H NMR、13C NMR和IR进行了表征。光谱分析实验结果表明,相比于其他探针,探针L2对Zn2+具有更好的选择性和灵敏度,检出限为92.15 nmol·L-1。在0~30μmol·L-1的浓度范围内,探针L2的荧光强度与Zn2+浓度可呈良好的线性关系,在紫外灯下可用肉眼观察到明显颜色变化。通过Job曲线、核磁滴定实验、密度泛函理论计算和L2-Zn2+配合物单晶结构对识别机理进行了研究,结果表明探针L2与Zn2+以物质的量之比1∶1配位。此外,探针L2还可用于检测实际水样中的Zn2+。 相似文献
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以乙二胺与水杨醛及其衍生物为原料,构建了4种席夫碱Zn2+荧光探针,其结构均由1H NMR、13C NMR和IR进行了表征。光谱分析实验结果表明,相比于其他探针,探针L2对Zn2+具有更好的选择性和灵敏度,检出限为92.15 nmol·L-1。在0～30μmol·L-1的浓度范围内,探针L2的荧光强度与Zn2+浓度可呈良好的线性关系,在紫外灯下可用肉眼观察到明显颜色变化。通过Job曲线、核磁滴定实验、密度泛函理论计算和L2-Zn2+配合物单晶结构对识别机理进行了研究,结果表明探针L2与Zn2+以物质的量之比1∶1配位。此外,探针L2还可用于检测实际水样中的Zn2+。 相似文献
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以四苯乙烯为荧光基团,5-叔丁基-2-羟基苯作为识别基团,构建了一种新型的聚集诱导发光(AIE)效应Zn2+荧光探针L.探针结构通过NMR和ESI-MS表征,其荧光性能通过UV-vis和荧光光谱研究.在乙醇/磷酸盐(PBS)(V∶V=7∶3,pH=7.4)溶液中,探针对Zn2+表现出高灵敏度和选择性,其检测限低至34.1 nmol·L-1,在0～3.0×10-5mol·L-1范围内,探针对Zn2+表现出良好的线性关系.在自然光和紫外灯下用肉眼观察到明显颜色变化,可以实现Zn2+可视化检测.通过Job’splot,ESI-MS和密度泛函理论(DFT)理论计算对识别机理进行了研究.报道的新型探针可作为分析测定Zn2+的一种便捷工具. 相似文献
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Copper is the third most abundant essential transition metal ion in the human body. It's responsible for important activities in many living things, but excessive intake of Cu2+ can lead to a range of diseases. A colorimetric and turn-off fluorescent probe (E)-2-(5-(benzothiazol-2-yl)-2-(diethylamino)-4-hydroxybenzylidene)-N-phenylhydrazine-1-carbothioamide ( ZTR ) was designed and synthesized by thiosemicarbazone Schiff base as a specific complexes site strategy to achieve highly specific Cu2+ detection. The fluorescence of the probe ZTR solution fell dramatically when Cu2+ was added, and its appearance changed from dazzling blue to nearly colorless. The simple structure and readily available fluorescent probe provide a novel approach for the quantitative detection of Cu2+ in the linear range from 0 to 0.12 μM, with a detection limit down to 16 nM, and with high selectivity for Cu2+ over 15 other metal ions. Job’s plot analysis showed that probe ZTR and Cu2+ formed a 1:1 coordination complex. In addition, because of its low detection limits and fast response time, the created fluorescent molecule was effectively used to study the target ions on test paper strips and in water samples.)) 相似文献
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Yang Liu Haoying Yang Qian Liu Mingming Pan Danli Wang Shiyuan Pan Weiran Zhang Jinfeng Wei Xiaowei Zhao Junfeng Ji 《Molecules (Basel, Switzerland)》2022,27(4)
Chemo and siRNA synergic treatments for tumors is a promising new therapeutic trend. Selenocystine, a selenium analog of cysteine, has been considered a potential antitumor agent due to its redox perturbing role. In this study, we developed a nanocarrier for siRNA based on a selenocystine analog engineered polyetherimide and achieved traceable siRNA delivery and the synergic killing of tumor cells. Notably, we applied the label-free Schiff base fluorescence mechanism, which enabled us to trace the siRNA delivery and to monitor the selenocystine analogs’ local performance. A novel selenocystine-derived fluorescent Schiff base linker was used to crosslink the polyetherimide, thereby generating a traceable siRNA delivery vehicle with green fluorescence. Moreover, we found that this compound induced tumor cells to undergo senescence. Together with the delivery of a siRNA targeting the anti-apoptotic BCL-xl/w genes in senescent cells, it achieved a synergistic inhibition function by inducing both senescence and apoptosis of tumor cells. Therefore, this study provides insights into the development of label-free probes, prodrugs, and materials towards the synergic strategies for cancer therapy. 相似文献
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Ling-Wen Xu Xin-Tian Wang Yun-Hong Zou Xu-Ya Yu Cheng-Zhi Xie Xin Qiao Qing-Zhong Li Jing-Yuan Xu 《应用有机金属化学》2020,34(10):e5812
Excessive aluminum exposure in the human body has been held responsible for multiple adverse effects, and existing data underscore the significance of aluminum detection in environmental and biological systems. Developing high-performance Al3+ fluorescent chemosensors can revolutionize our understanding of the physiological and pathological processes of Al3+ ions. Herein, we reported a highly sensitive and selective Schiff base fluorescence sensor, bis-NAPPD (1,1'-((1E,1'E)-(pyridine-2,3-diylbis(azanylylidene))bis(methanylylidene))bis(naphthalen-2-ol)), which can recognize Al3+ ions and exhibits a remarkable turn-on dual emission response (by ~23 fold) with a low nanomolar level detection limit (1.67 × 10−8 M) in methanol. Furthermore, the binding behavior and the turn-on fluorescence probing mechanism of bis-NAPPD were illustrated in detail by UV–vis titration, 1H NMR, and ESI-MS spectroscopy as well as density functional theory calculations. Notably, bis-NAPPD showed great potential for tracing Al3+ distribution in cells and living zebrafish larvae, and can also be applied in the fluorimetric detection of aluminum in sucralfate tablets with good precision and satisfactory accuracy, which may represent a promising Al3+ probe in bioimaging and biomedical applications. 相似文献
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《Journal of Coordination Chemistry》2012,65(23):3836-3846
AbstractThe logK1 was quantified by four parameters, metal ion radii R, metal ion charge Q, metal atom polarizability P, and Hammett constants σ of substituent, where logK1 was the stability constant of complexes (179) involving 10 Schiff bases Sal-A-X (N-(5-chloro-4-6-dimethyl-benzylidene)-anilines) reacting with 18 metal ions. The effect of substituents on the coordination ability of Sal-A-X was systematically investigated, and an expression with good correlation was obtained. The result shows that the electronic effect of substituent X on the aniline ring has an important influence on the stability of the complex; its electron-donating effect enhances the stability of the complex, whereas its electron-withdrawing effect lowers the stability of the complex. The theoretical results provide a potential value for the design of coordination compounds with unique function and reactivity. Moreover, their coordination selectivity to specific metal-ions can also be evaluated according to Equation (2). 相似文献
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Ling-Yi Shen Xiao-Li Chen Xian-Jiong Yang Hong Xu Ya-Li Huang Xing Zhang Carl Redshaw Qi-Long Zhang 《Molecules (Basel, Switzerland)》2021,26(13)
A novel turn-on fluorescence probe L has been designed that exhibits high selectivity and sensitivity with a detection limit of 9.53 × 10−8 mol/L for the quantification of Zn2+. 1H-NMR spectroscopy and single crystal X-ray diffraction analysis revealed the unsymmetrical nature of the structure of the Schiff base probe L. An emission titration experiment in the presence of different molar fractions of Zn2+ was used to perform a Job’s plot analysis. The results showed that the stoichiometric ratio of the complex formed by L and Zn2+ was 1:1. Moreover, the molecular structure of the mononuclear Cu complex reveals one ligand L coordinates with one Cu atom in the asymmetric unit. On adding CuCl2 to the ZnCl2/L system, a Cu-Zn complex was formed and a strong quenching behavior was observed, which inferred that the Cu2+ displaced Zn2+ to coordinate with the imine nitrogen atoms and hydroxyl oxygen atoms of probe L. 相似文献
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4-(N,N-二乙氨基)水杨醛是染料及有机合成的重要中间体,也是具有亲水性质的生色团,其醛基常被用来制备席夫碱进而开发新颖的探针分子。本文介绍了对于一些常见离子如Al~(3+)、Cu~(2+)、Zn~(2+)、Hg~(2+)、Co~(2+)、F~-、CN~-等进行荧光识别和检测的基于4-(N,N-二乙氨基)水杨醛合成的席夫碱类荧光探针,这些探针具有较高的选择性和灵敏度,并有很好的细胞通透性和较低的细胞毒性,有一定的实用价值。本文为此类荧光探针在环境、生物、食品中的实际检测和应用研究提供了理论依据和实践基础。 相似文献
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生物体系里微量元素锌在发育、新陈代谢和疾病的发生等多个领域扮演了重要的角色,定性和定量测量锌的含量将帮助我们理解锌的生物学意义。我们偶然发现5,5-二乙烯基-2,2-联吡啶(DVBP)在二氧六环里对锌离子呈现出类似比例计量型荧光探针的特性:即有2个荧光发射峰而且长波长峰随锌离子的增加按比例增长。但DVBP难溶于水而且2个荧光发射峰距离太近从而相互干扰,我们尝试利用硅氢反应将DVBP固定在多孔硅上制备感应锌离子的光极,我们发现多孔硅固相载体上的联吡啶通过与锌离子的螯合后荧光增强约8倍,比DVBP在溶液里与锌离子螯合后的荧光增强(约4.5倍)还要多,联吡啶对锌离子的荧光响应是一个值得继续探索的领域。 相似文献