Surface enhanced Raman scattering of biospecies on anodized aluminum oxide films

Traditionally, aluminum and anodized aluminum oxide films (AAO) are not the platforms of choice for surface-enhanced raman scattering (SERS) experiments despite of the aluminum’s large negative permittivity value. Here we examine the usefulness of aluminum and nanoporous alumina platforms for detecting soft biospecies ranging from bacterial spores to protein markers. We used these flat platforms to examine SERS of a model protein (cytochrome c from bovine heart tissue) and bacterial cells (spores of Bacillus subtilis ATCC13933 used as Anthrax simulant) and demonstrated clear Raman amplification.     

Determination of DNA using sodium 9,10-anthraquinone-2-sulfonate as an in situ photochemical fluorescence probe

An in situ photochemical fluorescence probe method for the determination of DNA with sodium 9,10-anthraquinone-2-sulfonate (AQS) as a photochemical fluorescence probe was developed. It was based on the conversion of AQS into an intensively fluorescent product by irradiating with UV radiation. The photochemical reaction is retarded by DNA. The determination can be carried out by measuring the fluorescence intensity at a fixed time. The calibration graph was linear in the range 0–80 ng ml⁻¹ calf thymus (CT) DNA (r = 0.9991), the limit of detection was 3.2 ng ml⁻¹ CT DNA (n = 9). The kinetic behaviour of the photochemical reaction and the effects of experimental conditions were investigated and discussed in detail. The results of absorption spectra and competitive binding experiments suggested the interaction between AQS and DNA to be intercalative.     

Modification of PAE-degrading Esterase(CarEW) for Higher Degradation Efficiency Through Integrated Homology Modeling,Molecular Docking,and Molecular Dynamics Simulation

Six phthalate acid esters(PAEs) priority pollutants[dimethyl phthalate(DMP), diethyl phthalate(DEP), dibutyl phthalate (DBP or DNBP), di-n-octyl phthalate(DNOP), di 2-ethyl hexyl phthalate(DEHP), and butyl benzyl phthalate(BBP)] were opted as the research object. PAE-degrading esterase CarEW(PDB ID:1C7I) isolated from Bacillus subtilis acting as a template and an iterative saturation mutation strategy was adopted to modify key amino acids to attain efficient PAE-degrading esterase substitutes with a reasonable structure constructed by homology modeling method. Present study designed a total of 285 unit-site and multi-site substitutions of PAE-degrading esterase using the homology modeling method. Among them, 207 PAE-degrading esterase substitutions, which contained the 6-site PAE-degrading esterase substitute 1C7I-6-9 with 84.21% enhancement intensity of degradation ability revealed better degradability to all the 6 PAEs after modification. Moreover, molecular dynamics simulation based on the Taguchi method reported the optimal external application environment for PAE-degrading esterase substitutes as follows:pH=6, T=35℃, the rhamnolipid concentration was 50 mg/L, the molar ratio of nitrogen to phosphorus(N:P) was 10:1, the concentration of H₂O₂ was 50 mg/L, and the voltage gradient was 1.5 V/cm. The degradation ability of PAE-degrading esterase substitutes was found to be elevated by 13.04% as compared to that of the blank control under the optimal condition. Moreover, 11 highly efficient PAE-degrading esterase substitutes with thermal stability were designed.     

Effect of gamma radiation on growth and survival of common seed-borne fungi in India

The present work describes radiation-induced effects of major seeds like Oryza sativa Cv-2233, Oryza sativa Cv-Shankar, Cicer arietinum Cv-local and seed-borne fungi like Alternaria sp., Aspergillus sp., Trichoderma sp. and Curvularia sp. ⁶⁰Co gamma source at 25 °C emitting gamma ray at 1173 and 1332 keV energy was used for irradiation. Dose of gamma irradiation up to 3 kGy (0.12 kGy/h) was applied for exposing the seed and fungal spores. Significant depletion of the fungal population was noted with irradiation at 1–2 kGy, whereas germinating potential of the treated grain did not alter significantly. However, significant differential radiation response in delayed seed germination, colony formation of the fungal spores and their depletion of growth were noticed in a dose-dependent manner. The depletion of the fungal viability (germination) was noted within the irradiation dose range of 1–2 kGy for Alternaria sp. and Aspergillus sp., while 0.5–1 kGy for Trichoderma sp. and Curvularia sp. However, complete inhibition of all the selected fungi was observed above 2.5 kGy.     

Improvement of microbiological safety of sous-vide meals by gamma radiation

Experimental batches of smoked-cured pork in stewed beans sauce were inoculated with spores of psychrotrophic Bacillus cereus, more heat and radiation resistant than spores of non-proteolytic C. botulinum. After vacuum packaging, the meals were treated with combinations of pasteurizing heat treatments and gamma irradiation of 5 kGy. Prior and after treatments, and periodically during storage at 10°C, total aerobic and total anerobic viable cell counts, and selectively, the viable cell counts of B. cereus and sulphite-reducing clostridia have been determined. The effects of the treatment order as well as addition of nisin to enhance the preservative efficiency of the physical treatments were also studied. Heat-sensitization of bacterial spores surviving irradiation occurred. The quality-friendly sous-vide cooking in combination with this medium dose gamma irradiation and/or nisin addition increased considerably the microbiological safety and the keeping quality of the meals studied. However, approx. 40% loss of thiamin content occurred as an effect of combination treatments, and adverse sensorial effects may also limit the feasible radiation doses or the usable concentrations of nisin.     

ACTION SPECTRA IN ULTRAVIOLET WAVELENGTHS (150-250 nm) FOR INACTIVATION AND MUTAGENESIS OF Bacillus subtilis SPORES OBTAINED WITH SYNCHROTRON RADIATION

Bacillus subtilis spores were exposed in vacuo to monochromatic UV radiation from synchrotron radiation in the wavelength range of 150 nm to 250 nm. Survival and frequency of mutation to histidine-independent reversion were analysed for three types of spores differing in DNA-repair capabilities. UVR spores (wild-type DNA repair capability) exhibited nearly equal sensitivity to the lethal effects of far-UV (220 nm and 250 nm) and of vacuum-UV radiation (150 and 165 nm), but showed marked resistance to 190 nm radiation. UVS spores (excision-repair and spore-repair deficient) and UVP spores (a DNA polymerase I-defective derivative of UVS) exhibited similar action spectra; pronounced sensitivity at 250 and 220 nm, insensitivity at 190 nm and a gradual increase of the sensitivity as the wavelength decreased to 165 nm. In all strains, the action spectra for mutation induction paralleled those for the inactivation, indicating that vacuum-UV radiation induced lethal and mutagenic damages in the spore DNA. The insensitivity of the spores to wavelengths around 190 nm may be explicable by assuming that radiation is absorbed by materials surrounding the core in which DNA is situated.     

Synthesis of small crystal polycrystalline mordenite membrane

Mordenite membrane was prepared on -Al₂O₃ tube by in situ hydrothermal synthesis with tetraethylammonium bromide (TEABr) as template agent. By the application of aging process of the parent solution, the size of mordenite crystals could be remarkably reduced from 20–30 (without aging) to 4–5 μm. The small crystal mordenite membrane had higher performance of pure gas permeation than big crystal mordenite membrane. The ideal selectivity of H₂/N₂ was 9.80, much higher than 3.82 of big crystals mordenite membrane. The membrane displayed high water-permselective performance in pervaporation (PV) test toward water/organic liquid mixtures. The highest separation factors achieved toward water/methanol, water/ethanol, water/n-propanol and water/i-propanol were 2600 (X_W=50%, T=323 K), 5500 (X_W=50%, T=343 K), 6000 (X_W=15%, T=343 K) and 6800 (X_W=50%, T=343 K), respectively.     

Bilayer structure of tetrasodium thiacalix[4]arene tetrasulfonate

The title calixarene, tetrasodium thiacalix[4]arene tetrasulfonate, was prepared and its crystal structure was determined. Na₄[thiacalix[4]arene sulfonate]·9H₂O·CH₃CH₂OH, belongs to triclinic system, space group P , a=10.820(5), b=14.109(6), and c=14.514(6)Å, =99.702(7), β=93.445(8), and γ=93.445(8)°, V=2174.2(16)ų, Z=2. The title calixarene exists in the solid state as bi-layer of anionic calixarene in the cone configuration. These layers alternate with inorganic regions which contain the sodium cations and the water molecules.     

Preparation and X-ray crystal structure of hexakis(1-methylimidazoline-2(3H)-thione)tetrakis(nitrato)dilead(II)

The title complex, prepared by reacting lead(II) nitrate with a stoichiometric amount of 1-methylimidazoline-2(3H)-thione (mimt) in water, crystallizes in the triclinic space group P with a = 9.334(2), b = 11.340(4), c = 11.398(3) Å, = 108.24(2), β = 95.69(2), γ = 100.27(2)° and Z = 1. In the solid state, the molecules show an unusual dimeric structure, with the formula [(mimt)₂(NO₃)₂Pb(μ-mimt)₂Pb(NO₃)₂(mimt)₂].     

Reactions and X-ray crystal structure of (3-cyano-2,4-pentanedionato-N)pentaamminecobalt(III) perchlorate chloride dihydrate

The pentaamminecobalt(III) complex with the 3-cyano-2,4-pentanedionate anion coordinated through the nitrile nitrogen has been characterized by X-ray crystallography. The crystals of [(NH₃)₅CoNCacac](Cl)(ClO₄)·2H₂O are triclinic, space group P , a = 10.245(2) Å, b = 14.071(4) Å, c = 6.971(2) Å, = 90.03(3)°, β = 109.86(2)°, γ = 108.91(2)°, V= 887.1 ų, Z = 2, D_c = 1.64 g cm⁻³, F(000) = 456, Mo-K radiation, λ = 0.71069 Å, μ(Mo-K) = 12.7 cm⁻¹. The structure was determined by the heavy-atom method, and refined by block-diagonal least-squares calculations, R = 0.0537, R_w = 0.0607, for 2499 observed reflections. Principal dimensions are: Co---N(NH₃) trans to NCacac⁻ 1.940(5), other Co---N(NH₃) 1.967(2), Co---N(NCacac⁻) 1.911(5) Å. The pendant acac moiety is best described in terms of a delocalized bond network with, for example, C---C distances in the range 1.44–1.52(1) Å. Several reactions involving this free acac group are also described including the preparation and characterization of the dimeric species pentaamminecobalt(III) - μ - (3 - cyano - 2,4 - pentanedionato) - bis(propylenediamine) cobalt(III) perchlorate.     

Structural and spectroscopic properties of Hexamethylenetetramine cobalt(II) complex: [Co(NCS)2(hmt)2(H2O)2][Co(NCS)2(H2O)4] (H2O)

Synthesis, structure, spectroscopy and thermal properties of complex [Co(NCS)₂(hmt)₂(H₂O)₂][Co(NCS)₂(H₂O)₄] (H₂O) (I), assembled by hexamethylenetetramine and octahedral Co(II) metal ions, are reported. Crystal data for I: F_w 387.34, a=9.020(8), b=12.887(9), c=7.95(1) Å, =96.73(4), β=115.36(5), γ=94.16(4)°, V=820(1) Å³, Z=2, space group=P−1, T=173 K, λ(Mo-K)=0.71070 Å, ρ_calc=1.718567 g cm⁻³, μ=17.44 cm⁻¹, R=0.088, R_w=0.148. An interesting two-dimensional network is assembled via hydrogen bonds through coordinated and free water molecules. The d–d transition energy levels of Co(II) ion are determined by UV–vis spectroscopy and calculated by ligand field theory. The calculated results agree well with experiment ones.     

INACTIVATION ACTION SPECTRA OF BACILLUS SUBTILIS SPORES IN EXTENDED ULTRAVIOLET WAVELENGTHS (50–300nm) OBTAINED WITH SYNCHROTRON RADIATION

Five types of Bacillus subtilis spores (UVR, UVS, UVP, RCE, and RCF) differing in repair and/or recombinational capabilities were exposed to monochromatic radiations at 13 wavelengths from 50 to 300 nm in vacuum. An improved biological irradiation system connected to a synchrotron radiation source was used to produce monochromatic UV radiation in this extended wavelength range with sufficient fluence to inactivate bacterial spores. From the survival curves obtained, the action spectra for the inactivation of the spores were depicted. Recombination-deficient RCE (recE) and RCF (recF) spores were more sensitive than the wild-type UVR spores in the entire range of wavelengths. This was considered to mean that DNA was the major target for the inactivation of the spores. Vacuum-UV radiations of 125-175 nm were effective in killing the spores, and distinct peaks of the sensitivity were seen with all types of the spores. Insensitivities at 190 and 100 nm were common to all five types of spores, indicating that these wavelengths were particularly impenetrant and absorbed by the outer layer materials. The vacuum-UV peaks centering at 150 nm were prominent in the spores defective in recombinational repair, while the far-UV peaks at around 235 and 270 nm were prominent in the UVS (uvrA ssp) and UVP (uvrA ssp polA) spores deficient in removal mechanisms of spore photoproducts. Thus, the profiles of the action spectra were explained by three factors; the penetration depth of each radiation in a spore, the efficiency of producing DNA damage that could cause inactivation, and the repair capacity of each type of spore.     

伊曲茶碱中间体杂质的分离与鉴定

伊曲茶碱是一种新型选择性腺苷A_2A受体拮抗剂,用于治疗帕金森氏病和改善帕金森氏病初期运动障碍。在伊曲茶碱中间体A1(6-氨基-1,3-二乙基-2,4-(1H,3H)-嘧啶二酮)的合成过程中,碱性条件或高温条件下会伴随生成至少2种副产物,在前期研究中我们已经对该中间体合成过程中的其中一种副产物进行过研究,确定其结构为(E)-N-乙基-2-氰基-3-乙氨基-2-丁烯酰胺。本文采用高效液相色谱(HPLC)对中间体A1的另外一种杂质进行分析:称取0.4 g中间体放入50 mL的烧杯中,依次加入8 mL水、8 mL乙腈,超声溶解,经0.45 μm有机膜过滤,得到测试样品溶液。采用Agilent Zorbax C18色谱柱(150 mm×4.6 mm, 5 μm)分离,柱温35 ℃,流动相为乙腈(A)和水(B),梯度洗脱(t_min/A∶B)=t₀/20∶80, t₁₅/60∶40, t₂₀~t₅₀/90∶10;流速1.0 mL/min;检测波长268 nm。然后通过Ceres B制备色谱柱分离,以乙腈-水(30/70, v/v)为流动相,流速为30 mL/min,在268 nm波长下检测,洗脱得到杂质纯品。通过高分辨率质谱(HRMS)、一维核磁共振(NMR)、二维核磁共振(2D NMR)对杂质进行了结构确认,并通过单晶X射线衍射(XRD)进行了表征。杂质经分析确认为1-(1,3-二乙基-2,6-二氧-1,2,3,6-四氢嘧啶-4-基)-3-乙基脲。根据杂质的化学结构推测其生成机理为:在碱性条件或高温条件下合成中间体A1时,过量的二乙基脲继续与中间体A1发生酰胺化反应而得到此副产物。此杂质与伊曲茶碱中间体A1结构相似,会伴随A1参与到伊曲茶碱合成的后续反应中,并对伊曲茶碱的安全性和有效性产生潜在的影响。因此,为了确保伊曲茶碱的质量,在生产过程中需要对该杂质的含量进行控制。     

Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics

We investigated femtosecond pump–repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270 nm-pump 810 nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (Bacillus subtilis, Escherechia coli and Enterococcus fæcalis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.     

EFFECT OF DIPICOLINIC ACID ON THE ULTRAVIOLET RADIATION RESISTANCE OF BACILLUS CEREUS SPORES

Abstract— The ultraviolet radiation (UV) resistance of B. cereus spores was shown to depend on their content of dipicolinic acid (DPA). Wild-type spores with decreasing amounts of DPA exhibited increased UV resistance. Similarly, spores devoid of DPA (DPA-minus), produced by a mutant strain of B. cereus unable to synthesize DPA, were more resistant to UV than mutant spores (DPA-plus) produced in the presence of exogenously supplied DPA. Resistance of both the wild type and mutant strains to ionizing radiation, however, was unaffected by DPA content. Comparison of the resistance of DPA-minus and DPA-plus mutant spores to UV of various wavelengths showed that the greater sensitivity of the latter DPA-plus spores appeared at wavelengths corresponding to the region of the first molecular absorption band of the calcium chelate of DPA. In the wild type and mutant, thymine photoproducts were produced at a greater rate and to a greater extent in spores with high levels of DPA than in spores with low DPA.
The data indicate that DPA transfers energy to DN A in vivo , which leads to the conclusion that DPA occurs in the spore protoplast.     

Crystal Structure, Nano-particle Morphology and Interaction with ATP of [ NH3 CH2 CH（ NH2 ） CH3 ]2 [ M（ Ⅵ ） O2 （ OC6 H4 O）2 ] （M = Mo0. 6 W0. 4 ） and Its Activity to Promote DNA Cleavage

The title complex [NH_3CH_2CH(NH_2)CH_3]_2 [M(Ⅵ)O_2(OC_6H_4O)_2](M= Mo_(0.6)W_(0.4))was synthesized via a simple solution-phase chemical route.The determination of single crystal X-ray diffraction revealed that the title compound is crystallized in a monoclinic system with P2(1)/n space group,a=1.0913(10)nm,b=1.0442(10)nm,c=1.8842(19)nm,α=90°,β=96.530(17)°,γ=90°,Z=4,and V=2.133(4)nm3.The mononuclear anionic unit [M(Ⅵ)O2(OC6H4O)2]2-displays chiral pseudo-octahedral [MO_6] coordination geometry and is linked by chiral cations via hydrogen bond and π…π stacking interaction.The transmission electron microscopy images show that the title complex is comprised of nano-particles with diameters ranging from 20 to 50 nm.The NMR study shows the 1H downfield chemical shifts of [NH_3CHaHbCH(NH_2)CH_3] cations in the title complex when it is mixed with adenosine-triphosphate(ATP),and the chemical shift difference between Ha and Hb is increased greatly,and most of the catecholate ligands dissociate from the central metal atoms.The DNA cleavage activity experiment reveals that DNA cleavage promoted by the title complex is lower than that by Na_2MoO_4 which possesses antitumor pro-perty,but higher than that by Na_2WO_4.     

Monitoring the moisture-related degradation of ethylene propylene rubber cable by electrical and SEM methods

Several electrical approaches were used to monitor the electrical degradation of ethylene propylene rubber (EPR) cable under (1) high temperature water submerged for long-term, (2) room temperature water submerged after thermal and radiation aging, and (3) loss-of-coolant accident simulation conditions. The moisture-related degradation behavior was investigated by the correlation of electrical measurements, moisture content analysis, and scanning electron microscope (SEM) observation. The results indicate that void size and density of insulation material strongly affect the material's water resistant capability and electrical degradation. Also, the void size of insulation material after moisture-related degradation is much bigger than that after thermal and radiation aging, and the former causes material swelling. Combining insulation resistance and dissipation factor condition monitoring (CM) techniques can provide useful means for monitoring cable degradation while the cable is exposed to moisture and high temperature/radiation environment. The Arrhenius model is applied together with appropriate acceptance criteria obtained by insulation resistance measurement to calculate cable remaining life. According to test results and the remaining life calculation, it is shown that the moisture-related degradation of EPR cable can be early assessed by insulation resistance measurement, which is a non-destructive prognostic CM technique.     

Synthesis, characterization, and X-ray crystal structures of W(VI) alkyl complexes with chelating diamide and imido co-ligands

The complex W(NPh)Cl₂[o-(NSiMe₃)₂C₆H₄] 3 was synthesized from PhN = WCl₄ · OEt₂ and N,N′-(Li₂[o-(NSiMe₃)₂C₆H₄] and reacts with Lewis bases to form the adducts W(NPh)Cl₂[o-(NSiMe₃)₂C₆H₄](L) (L = PMe₃, THF, 3-picoline, ^tBuNC, MeCN) 4a–e. Crystals of 4a are triclinic, space group P1¯, with a = 9.562(1), b = 10.277(1), c = 14.920(2) Å, = 82.15(1), β = 80.18(1), γ = 80.41(1)°, and Z = 2. The structure was solved by the heavy atom method and refined to R = 0.0408 for 4224 observed (I > 2σ(I)) reflections. The dialkyl complexes W(NPh)R₂[o-(NSiMe₃)₂C₆H₄] (R = Me, Et, CH₂Ph, CH₂CMe₃, CH₂CMe₂Ph) 5–9 are formed through subsequent reactions of 3 with the corresponding Grignard reagent. Crystals of complex 5 are monoclinic, space group P2(1)/n, with a = 10.3545(2), b = 17.9669(1), c = 13.3168(1) Å, β = 103.826(1)°, and Z = 4. The structure of complex 5 was solved by direct methods in SHELXTL5 and refined to R = 0.0247 for 4572 observed reflections. Compound 5 has a square pyramidal geometry in which the imido ligand occupies the apical position and reacts with PMe₃ to form the adduct W(NPh)Me₂[o-(NSiMe₃)₂C₆H₄](PMe₃) 5a. Crystals of complex 5a are monoclinic, space group C2/m, with a = 13.5336(1), b = 14.4291(1), c = 15.3785(1) Å, β = 110.365(1)°, and Z = 4. The structure of compound 5a was solved by direct methods in shelxtl5 and refined to R = 0.0272 for 3057 observed reflections. Crystals of the bis-neopentyl complex 8 are monoclinic, space group P2(1)/n, with a = 10.6992(4), b = 18.3144(7), c = 16.0726(6) Å, β = 92.042(1)°, and Z = 4. The structure of 8 was solved by direct methods in shelxtl5 and refined to R = 0.0261 for 5881 observed reflections. Complex 8 has a trigonal bipyramidal geometry with both neopentyl groups and one amido nitrogen in the equatorial plane.     

层状磷酸铝化合物Na4[Al4P4O18]·H2O的合成及质子电导性能研究

在无有机模板剂的条件下, 以Na⁺离子为结构导向剂, 通过水热合成法制备了一种与利用1,3-丙二胺合成的Uio-14具有相同层结构的二维磷酸铝化合物Na₄[Al₄P₄O₁₈]·H₂O(1), 通过单晶X射线衍射确定了其拓扑结构. 利用粉末X射线衍射、 扫描电子显微镜、 电感耦合等离子体( ICP)元素分析和热重分析等对其物理化学性质进行了表征. 结果表明, 化合物1属于单斜晶系, 空间群为P2₁/c, 晶胞参数a=1.00887(9) nm, b=0.86747(8) nm, c=0.97580(9) nm, V=0.77387(12) nm³, Z=2, 其阴离子层由铝氧三角双锥(AlO₅)和磷氧四面体(PO₄)构成, 层间通过Na⁺离子平衡电荷; 与Uio-14相比, 化合物1具有更高的热稳定性, 在400 ℃空气条件下煅烧后结构仍然保持完好. 对化合物1的质子电导性能测试结果表明, 相比于传统的分子筛类材料, 化合物1展现出优异的质子电导性能, 在55 ℃下质子电导率可达到1.19×10^-3 S/cm.     

The synthesis and x-ray structure of (N,N-dimethyldithiocarbamato)(n-butyl)diphenyltin(IV)

(N,N-Dimethyldithiocarbamato)(n-butyl)diphenyltin(IV), n-BuPh₂SnS₂NMe₂, crystallizes in the monoclinic space group P2₁/n with a 9.772(5), b 9.895(4), c 21.418(9) Å, β 95.81(3)⁰, V 2060 ų Z = 4, μ 14.4 cm⁻¹ The structure was determined by the heavy-atom technique from 3103 independent reflections measured at room temperature on an Enraf-Nonius four-circle CAD-4 diffractometer using monochromatized Mo-K radiation and refined to a final R value of 5.8%. The tin atom is essentially four-coordinated with a weak fifth tin-sulphur bond (Sn---S(2) 3.079(1) Å) considerably longer than the other (Sn---S(1) 2.466(1) Å). A comparison with the complex n-BuPhSn(C1)S₂CNEt₂ (Sn---S(1) 2.454(1) Å; Sn---S(2) 2.764(1) Å) suggests that enhanced steric factors are responsible for the preferential monodentate behaviour of the dithiocarbamate ligand in the title complex.