葡萄球菌肠毒素B分子印迹聚合物的制备及其与蛋白质的结合性能

以葡萄球菌肠毒素B(SEB)蛋白为模板分子,以聚苯乙烯微球为基质,采用表面分子印迹法制备了SEB分子印迹聚合物.利用平衡吸附试验分析了SEB聚合物对目标蛋白的吸附能力及对类似底物的选择性;分析了该聚合物的吸附动力学,并利用扫描电镜观察了其形貌特征和颗粒尺寸.结果表明,经Scatchard模型分析求得的标题聚合物的最大表观结合量Qmax为3.23mg/g;所制备的SEB分子印迹聚合物呈微球形,粒径约为1²μm,对SEB蛋白具有较好的吸附性和特异选择性.     

核-壳型赤藓红分子印迹聚合物的制备及吸附性能的评价

以氨基化修饰的SiO_2为内核,人工合成色素赤藓红为模板,甲醇/水为溶剂,4-乙烯基吡啶为功能单体,二甲基丙烯酸乙二醇酯为交联剂,偶氮二异丁腈为引发剂,采用表面印迹技术,制备核-壳型赤藓红分子印迹聚合物。通过红外光谱对其结构进行表征,并通过动力学吸附、等温饱和吸附和实际样品加标实验对其吸附性能进行评价。结果表明,核-壳型赤藓红分子印迹聚合物具有较快的吸附能力,在15min左右达到吸附平衡,有较好的吸附容量,能够从复杂的食品样品中选择性吸附模板,且回收可达85%。     

硅胶表面亮菌甲素分子印迹聚合物的制备及其性能研究

采用光接枝印迹方法,在硅胶微球表面制备了以亮菌甲素为模板分子、2-乙烯基吡啶为功能单体的分子印迹聚合物,采用荧光法优选了功能单体及比例,进一步用荧光法对印迹聚合物的吸附特性和印迹效率进行评价.结果表明.该印迹聚合物对模板分子具有特异吸附性能,印迹效率为48.6%.     

Polyacrylamide gel beads for the recognition of staphylococcal enterotoxin B

Protein‐imprinted polyacrylamide gel beads (IPGB) were synthesized via inverse suspension polymerization, using staphylococcal enterotoxin B (SEB) as template. The adsorption capacity of SEB‐IPGB was almost three times as much as that of non‐imprinted gel beads. The Langmuir adsorption models were applied to describe the equilibrium isotherms. The results showed that an equal class of adsorption was formed in the SEB‐IPGB with the maximum adsorption capacity of 8.40 mg SEB/g imprinted beads. The selectivity test of imprinted beads shows that they exhibited good recognition for SEB as compared with the other proteins. The formation of multiple hydrogen bonds and complementary shape between the imprinting cavities and the template proteins would be the two factors that led to the imprinting effect. The obtained SEB‐IPGB would be used as a potential material for protein toxin separation, extraction, and purification. Copyright © 2014 John Wiley & Sons, Ltd.     

左氧氟沙星印迹聚合物微球及其特异吸附研究

以甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用沉淀聚合法制备了左氧氟沙星印迹聚合物(MIP)微球,用SEM和IR分别对微球形貌和结构进行表征,用静态吸附法和Scatchard分析考察了聚合物微球对模板及其结构类似物的吸附行为和选择性识别能力.结果表明,MIP对模板分子的吸附平衡速度快,40min即可达到吸附...     

环丙沙星分子印迹聚合物的合成及识别性能研究

采用分子印迹技术合成了以环丙沙星为印迹分子,以甲基丙烯酸和4-乙烯基吡啶同时为功能单体的分子印迹聚合物。运用平衡结合实验研究了印迹聚合物的吸附特性和选择识别能力。Scatchard分析表明,在所研究的浓度范围内,分子印迹聚合物中形成了两类不同的结合位点。底物选择实验表明,这种聚合物对环丙沙星呈现高的选择结合能力。     

3-氨基苯硼酸为功能单体在壳聚糖上印迹牛血清白蛋白的研究

以壳聚糖为载体, 3-氨基苯硼酸为功能单体对牛血清白蛋白进行了分子印迹的研究, 并对吸附过程进行Langmuir等温吸附模型的数据处理. 结果表明, 印迹聚合物上形成了对于模板分子有较高的吸附容量和选择性的识别位点, Langmuir等温吸附平衡常数为49.5 mL/mg, 结合位点的最大表观结合量为16.3 mg/g, 证明了该印迹聚合物对于牛血红蛋白和溶菌酶这些非模板蛋白的吸附不具有选择性.     

Synthesis of molecularly imprinted polymer with 7-chloroethyl-theophylline-immobilized silica gel as template and its molecular recognition function

By reaction of 7-chloroethyl-theophylline with aminopropylsilanized silica gel we synthesized a 7-chloroethyl-theophylline-immobilized silica gel as template molecule and prepared a molecularly imprinted polymer (MIP-Si), which had special recognition sites to 7-chloroethyl-theophylline. A conventional molecularly imprinted polymer (MIP) using 7-chloroethyl-theophylline as template was also prepared for comparison. Binding abilities to 7-chloroethyl-theophylline and its structural analogs revealed that the MIP-Si shows much higher binding speed and much more binding capacity than the MIP does.     

硅胶表面苯并噻吩分子印迹聚合物的分子识别与吸附性能

选用γ-氨丙基三乙氧基硅烷和α-甲基丙烯酸修饰的硅胶作为载体,以甲基丙烯酸为功能单体,二甲基丙烯酸乙二醇酯为交联剂,苯并噻吩为模板分子,合成一种具有选择性识别苯并噻吩分子的印迹聚合物。采用红外光谱、元素分析及N2吸附对其结构进行了表征,以模拟汽油通过静态吸附对其吸附性能进行了研究。结果表明,在硅胶载体表面成功地嫁接了多孔的分子印迹聚合物薄层。印迹聚合物对苯并噻吩具有良好的识别性能,对苯并噻吩的吸附动力学满足Langergren准一级反应动力学方程,吸附过程属于单分子层吸附。符合Langmuir吸附模型印迹聚合物对苯并噻吩的平衡吸附容量达57.4×10-3,而非印迹聚合物的吸附容量为33.1×10-3。印迹聚合物在经过多次再生后其吸附容量基本不变,从而为在汽油深度脱硫中有效脱除噻吩类硫化物提供了一种新技术途径。     

Selective recognition of 4-nitrophenol from aqueous solution by molecularly imprinted polymers with functionalized tetratitanate whisker composites as support

Three kinds of molecularly imprinted polymers (MIPs) were obtained with surface molecular imprinting technique on functionalized potassium tetratitanate whisker (F-PTW). The results of adsorption experiments indicated that MIP prepared using PTW modified with N-(2-aminoethyl)-3-(trimethoxysilyl)propylamine (AAPTS) (F-PTW A) as support [MIP(1)] was superior to the other two polymers, then MIP(1) was selected to analyze the 4-nitrophenol (4-NP) adsorption process from aqueous solution in this study. AAPTS offered hydrophilic exterior that allowed to self-assemble with the template 4-NP through intermolecular interaction rather than based on the interactions between the functional monomers and template. Equilibrium adsorption data were analyzed by the Langmuir and Freundlich isotherm models at various temperatures. Kinetic properties were successfully investigated by pseudo-first-order model, pseudo-second-order model, intraparticle diffusion equation, initial adsorption rate, half-adsorption time. A diffusion-controlled process as the essential adsorption rate-controlling step was also proposed. The performance of such imprinted polymer was further demonstrated by high-performance liquid chromatography, and the results showed that the selectivity of MIP(1) exhibited higher affinity for template 4-NP over competitive phenolic compounds than that of non-imprinted polymer NIP(1). MIP(1) could be reused four times without significant loss in the adsorption capacity.     

分子印迹固相萃取/高效液相色谱法分离和富集枳实中4种黄烷酮

采用沉淀聚合法以橙皮素为模板分子,2-乙烯基吡啶为功能单体,二甲基丙烯酸乙二醇酯为交联剂,合成了橙皮素分子印迹聚合物。利用紫外光谱法确定了最佳功能单体与配比,优化了合成条件。采用傅立叶变换红外光谱、扫描电子显微镜、静态吸附对聚合物进行表征。实验结果表明,分子印迹聚合物的吸附性能明显优于空白印迹聚合物,且此聚合物对柚皮苷、橙皮苷、柚皮素和橙皮素的相对选择系数分别为1.40,1.39,1.59和2.89,表明该分子印迹聚合物对4种黄烷酮有较好的选择性。将印迹聚合物作为固相萃取填料,对枳实提取液进行分离和富集,结果表明上述4种黄烷酮的提取率分别为72.6%,61.1%,95.4%和93.5%,分离富集效果良好,大大提高了枳实中4种黄烷酮的提取效率。     

反乌头酸分子印迹聚合物微球的制备及其分子识别功能

以乙腈为分散剂,采用沉淀聚合法合成了反乌头酸分子印迹聚合物微球。研究了合成反应条件对聚合物形貌的影响,发现聚合前主客体氢键络合物和功能单体氢键低聚体是控制微球形成及其粒径大小的关键因素。通过振荡吸附法对聚合物的结合特性进行了评价,发现印迹聚合物微球对模板分子的识别选择性优于块状印迹聚合物和非印迹聚合物。     

Via protoporphyrin to the synthesis of levofloxacin‐imprinted polymer

A new molecularly imprinted polymer (MIP) for levofloxacin was prepared by the combined use of methacrylic acid and protoporphyrin as functional monomers. The adsorption properties of resultant imprinted polymers were evaluated by equilibrium rebinding experiments. The highest binding capacity of levofloxacin achieved from the optimized imprinted polymer in acetonitrile was 246.26 µmol/g with an imprinting factor of 2.05. A ?uorescence quenching effect was observed when a protoporphyrin‐based imprinted polymer was incubated in the solutions of levofloxacin. The results indicated that the protoporphyrin‐based MIPs were able to create higher binding cavities for template compared with MIPs using only methacrylic acid as a functional monomer. It should be expected that the cooperative use of the protoporphyrin with supplemental different functional monomers may be an alternative to obtain MIP with the improvement of the selectivity. Copyright © 2010 John Wiley & Sons, Ltd.     

粉煤灰基氧氟沙星分子印迹聚合物的制备及其应用

以工业废料粉煤灰微球为基质,氧氟沙星 (OFL) 为模板分子,采用表面印迹法制备印迹材料MIP。通过紫外光谱法结合理论分析选择实验条件,并对该印迹材料结构、吸附行为进行研究。结果表明,该印迹材料对氧氟沙星具有良好的特异识别性和优良的亲和性。与以硅胶为载体制备的印迹聚合物相比,该材料吸附容量更高和印迹效果更好。将其作为固相萃取填料对鸡产品进行分离富集,与C18柱相比,分离富集效果更好。结合UPLC,对实际样品中氧氟沙星进行分析,回收率为82.0%-96.7%,相对标准偏差低于5.5%,可用于鸡产品中氧氟沙星分离分析。     

尼卡地平分子聚合物的制备及识别性能研究

采用分子印迹技术合成了以尼卡地平为模板分子,甲基丙烯酸为功能单体的分子印迹聚合物(MIP).运用平衡结合实验研究了聚合物的吸附特性和选择识别能力.通过Scatchard方程分析,结合位点的离解常数Kd=1.03 mmol·L-1,最大表观结合常数Qmax=18.76 μmol·g-1.结果表明,分子印迹聚合物对尼卡地平呈现出较高的吸附性和选择识别性,对尼卡地平药物的分离富集和检测具有实际临床意义.     

Synthesis of molecularly imprinted polymer nanoparticles for the fast and highly selective adsorption of sunset yellow

Novel molecularly imprinted polymer nanoparticles were synthesized by precipitation polymerization with sunset yellow as the template and [2‐(methacryloyloxy)ethyl] trimethylammonium chloride as the functional monomer. The molecularly imprinted polymer nanoparticles were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, and their specific surface area and thermal stability were measured. The molecularly imprinted polymer nanoparticles had a high adsorption capacity in wide pH range (pH 1–8) for sunset yellow. The adsorption equilibrium only needed 5 min, and the quantitative desorption was very fast (1 min) by using 10.0 mol/L HCl as the eluant. The maximum adsorption capacity of the molecularly imprinted polymer nanoparticles for sunset yellow was 144.6 mg/g. The adsorption isotherm and kinetic were well consistent with Langmuir adsorption model and pseudo‐second‐order kinetic model, respectively. The relative selectivity coefficients of the molecularly imprinted polymer nanoparticles for tartrazine and carmine were 9.766 and 12.64, respectively. The prepared molecularly imprinted polymer nanoparticles were repeatedly used and regenerated ten times without significant absorption capacity decrease.     

Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins

A new approach is reported on the use of poly(N-isopropylacrylamide) (PNIPAM)-coated molecularly imprinted beads (coated MIP beads) for controlling the release of protein. The coated MIP beads were composed of double layers, an internal thermosensitive lysozyme-imprinted layer, and an external PNIPAM layer. The coated MIP beads were prepared by two-step surface-initiated living-radical polymerization (SIP). In this systemic study, the coated MIP beads had good selectivity to the template protein (lysozyme) and temperature stimulus-responsive behavior, both of which were superior to those of MIP beads having a layer of thermosensitive lysozyme-imprinted polymer only. Using the coated MIP beads, reference proteins and the template lysozyme could be released separately at 38 °C and at 23 °C. The corresponding coated non-imprinted beads (coated NIP beads) did not have such double thermosensitive “gates” with specific selectivity for a particular protein. The proposed smart controlled imprinted system for protein is attractive for chemical carriers, drug-delivery system, and sensors.     

沉淀聚合制备诺氟沙星-Zn2+分子印迹聚合物及其印迹方式的研究

采用沉淀聚合的方式以诺氟沙星(NFA)-Zn2+为模板分子,乙二醇二甲基丙烯酸酯为交联剂,分别选取酸性功能单体甲基丙烯酸与碱性功能单体4-乙烯基吡啶制备了诺氟沙星-Zn2+的分子印迹聚合物.通过紫外光谱研究发现诺氟沙星与Zn2+及两种功能单体均发生了金属配位印迹作用且形成了比例不同的印迹复合物.红外光谱的功能基团的表征结果显示,甲基丙烯酸与诺氟沙星-Zn2+形成了以诱导作用占优的三元配合物,而4-乙烯基吡啶则与诺氟沙星-Zn2+形成了共轭作用占优的三元配合物.扫描电镜及粒径分布实验表征了聚合物的物理特性,结果显示印迹聚合物的表面存在孔及孔道结构而非印迹聚合物的表面较致密不存在孔且制备的印迹聚合物的粒径均在100μm以下,其平均粒径为39μm.等温结合及选择性实验的结果表明4-乙烯基吡啶为功能单体制备的印迹聚合物的选择性识别性能优于甲基丙烯酸为功能单体制备的印迹聚合物,其特异性吸附容量和印迹指数分别为66.84μmol/g和4.207.同时在混合溶液的选择性实验中以4-乙烯基吡啶(4-VP)为功能单体制备的印迹聚合物的选择识别诺氟沙星的能力优于以甲基丙烯酸为功能单体的聚合物,其识别因子分别为3.408和2.909,而非印迹聚合物对底物的吸附量较小且识别因子均接近于1,说明非印迹聚合物对底物的识别为非选择性的.     

Synthesis and Evaluation of Molecularly Imprinted Polymers as Sorbents for Selective Extraction of Coumarins

Coumarin, 7-hydroxycoumarin and dicoumarol molecularly imprinted polymers (MIP) were synthesized by bulk polymerization. Methacrylic acid and 4-vinylpyridine were tested as functional monomers and methanol, ethanol, acetonitrile, toluene and chloroform were tested as porogens. The binding capabilities of the imprinted polymers were assessed by equilibrium binding analysis. Highest binding capacity was obtained for MIP prepared for the template 7-hydroxycoumarin synthesized in methacrylic acid as functional monomer, chloroform as porogen and methanol/water as analyte solvent. Scanning electron microscopy analysis documented its appropriate morphology. ATR-FTIR spectra confirmed successful polymerization of MIP. Coumarin structural analogues were employed to evaluate the polymer selectivity and it was found that polymer prepared for 7-hydroxycoumarin was selective for its template molecule. Kinetic studies showed relatively fast adsorption of analytes to MIPs (1 h). Rebinding properties of MIPs were evaluated by adsorption isotherms. The calculated data fitted well with experimental data showing that Freundlich isotherm is suitable for modelling the adsorption of tested coumarins on prepared MIPs. Applicability of polymer prepared for 7-hydroxycoumarin was tested for the selective extraction of coumarins from the sample of chicory.     

分子印迹聚合物的制备及其对香草醛的吸附行为

以香草醛为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂,制备了香草醛分子印迹聚合物(MIP);研究了以不同致孔剂合成的MIP在水溶液中对香草醛的吸附行为.结果表明,以乙腈为致孔剂合成的MIP对香草醛具有较高的识别特性,能较简便地用于香草醛的分离和富集.