多状态旋转阀的研制及其在自动分析中的应用

本文研制了在流动注射分析及其它自动分析中应用的８状态旋转阀。该阀用步进电机驱动，光电开关定位，单片微机系统控制。所研制的阀应用于自动溶剂萃取光度分析，取得满意效果。     

激光共聚焦显微技术在共定位应用中的常见问题

荧光共定位分析是当今生物显微成像中一个极为常见的技术。本文从以下4个方面详细介绍了使用Zeiss LSM880型激光扫描共聚焦显微镜(confocal laser scanning microscope,CLSM)进行共定位研究时常见的问题及解决方法:1.如何用共聚焦进行共定位实验;2.如何进行共定位分析;3.如何评价共定位;4.如何判定共定位结果。在共定位研究中,只要按照共定位实验的基本要求拍摄图像,并按照上述方法进行分析,便可得到准确可靠的共定位分析结果。     

双帽Keggin型杂多阴离子[H4As3Mo12O40]^-和Keggin型杂多酸HaPM12O40（M=Mo，W）质子化的DFT研究

选用B3LYP方法在LanL2MB水平下，对双帽α—Keggin型杂多阴离子[H4As3Mo12O40]^-的电子结构和质子的定位进行了密度泛函理论（DFT）研究．结果表明，双帽的形成大大影响了杂多阴离子[As3Mo12O40]^5-的电子结构和性质，NBO分析显示参与成帽的三桥氧上的电子密度比双桥氧上的要大，简单地从电荷密度来看，质子将首先在三桥氧上定域成键，但通过比较质子定域在几种桥氧上质子化稳定化能的大小，发现[H4As3Mo12O40]^-中的四个质子将在八个双桥氧中的其中四个氧原子上定位，而不是如文献中报道的在四个三桥氧上定域成键．对杂多酸H3PM12O40（M=Mo，W）中质子的定位也进行了理论计算并与文献进行了比较，结果显示，H3PMo12O40中质子是定位在双桥氧上；而H3PW12O40中质子将优先在双桥氧上定位，但也可在端氧上定位；这一结果与文献报道的相一致。     

3,4—亚甲二氧基正丙基苯的氯甲基化反应

在季铵盐四乙基溴化铵存在下，３，４－亚甲二氧基正丙基进行氯甲基化反应的转化率达９６．６％，定位选择性达１００％，对产物进行了气相色谱，红外光谱，核磁共振测定及元素分析。     

固定化脂肪酶活性的X射线微区分析

利用X射线微区分析的方法，对吸附交联法得到的固定化脂肪酶的微观活性进行了分析。结果表明：以合成出的大孔吸附树脂为固定化酶栽体，酶活较高，活性脂肪酶分布较均匀。并得到了固定化脂肪酶的活性定位的最佳条件。     

从春小麦喷施稀土十年定位试验看稀土的残留

本文对春小麦喷施稀土十年定位试验的小麦籽粒，土壤中的稀土和放射性含量进行了分析，证明长期使用稀土后土壤，小麦籽粒中的稀土和放射性含量与对照样相比没有明显的区别，说明稀土农用不仅效益显著，而且是安全的。     

专利

一种光谱分析样品的定位装置公开号:CN201935864U公开日:2011.08.17申请人:东莞市亿铖达焊锡制造有限公司摘要:本实用新型公开了一种光谱分析样品的定位装置,用在光谱分析仪的工作台上,其中包括一定位板;在定位板上设置有缺口;缺口的侧面为平面。由于采用了在定位板上设置有缺口,通过缺口的侧平面定位在分析面的圆形外壁上,由此在分析时,配合试样的待分析面的圆形棱边与阳极的圆     

一种光谱分析样品的定位装置

公开号：CN201935864U公开日：2011．08．17申请人：东莞市亿铖达焊锡制造有限公司摘要：本实用新型公开了一种光谱分析样品的定位装置,用在光谱分析仪的工作台上,其中包括一定位板;在定位板上设置有缺口;缺口的侧面为平面。由于采用了在定位板上设置有缺口,通过缺口的侧平面定位在分析面的圆形外壁上,由此在分析时,配合试样的待分析面的圆形棱边与阳极的圆锥形锥面相接触,从而不需要操作人员准确观察并设置激发点的准确位置,提高了试样或样品的定位准确性,缩短了分析面的取样过程和时间。     

放射增敏性导弹型卟啉的合成与性质研究

用化学方法成功地将放射增敏剂灭滴灵连接到对癌细胞具有定位作用的原卟啉上，通过红外光谱、紫外可见吸收光谱、质谱、核磁共振谱和元素分析等手段确证了其结构。经动物实验证明，放射增敏效果较好。     

ECT脑血流灌注显像、CT和MRI在难治性癫痫中的诊断价值分析

选择2013年10月~2018年12月于我院收治的53例经临床确诊的难治性癫痫患者临床资料，所有患者均于发作间期分别行常规CT、MRI、SPECT检查，观察3种方法对致痫病灶检出和定位准确率，探讨单光子发射计算机断层成像术（SPECT）、CT和MRI在难治性癫痫中的诊断价值。结果显示，SPECT检测患侧rCBF低于健侧[（46.05±7.62）vs（54.51±9.39），P<0.05]，MRI检测患侧NAA（cho+Cr）低于健侧[（0.47±0.05）vs（0.69±0.11），P<0.05]。SPECT对致痫灶检出率、定位准确率分别为81.13%、73.58%，均高于MRI和常规CT（P<0.05）。联合SPECT、CT、MRI对致痫灶检出率、定位准确率达94.34%、92.45%，高于单独诊断（P<0.05），联合rCBF+NAA（cho+Cr）预测致痫病灶位置的AUC达0.923，灵敏度和特异度达82.26%、98.91%，高于rCBF、NAA（cho+Cr）（P<0.05）。结论：PET-CT、MRI对难治性癫痫术前定位均有较高临床应用价值，PET-CT在致痫灶检率、定位定侧方面更有优势，联合诊断更有助于提高定位准确率。     

Studies on Surface Plasmon Resonance Sensors in Optical Immunosensing

A novel optical biochemical sensor based on surface plasmon resonance (SPR) has been developed for immunosensing. The new device is a relatively simple and inexpensive one designed on the basis of fixing angle of incidence and scanning wavelength in the range of 400-800 nm. While the BIAcore SPR sensing method is to fix wavelength and modulate angle of incidence light, which need an expensive and complicated apparatus.     

Synthesis and Insecticidal Activity of Nitenpyram Analogs with Tetrahydropyrimidine Fixed (Z)‐Configuration

Two series of novel (Z)‐nitenpyram analogs 2a , 2b , 2c , 2d , 2e , 2f , 2g , 2h , 3a , 3b , 3c , 3d were synthesized by introducing various amino acids benzyl ester or amino acids tetrahydrofurfur‐2‐yl ester into nitenpyram and forming a tetrahydropyrimidine ring to fix (Z)‐configuration. The structures of the target compounds were characterized by ¹HNMR, MS, IR, and elemental analysis. Preliminary bioassays against Nilaparvata lugens indicated that all the nitenpyram analogs exhibited good insecticidal activity at 100 mg/L, whereas the compounds 3b and 3d afforded the best in vitro inhibitory activities that had ≥ 90% mortality at 20 mg/L.     

Rapid simple cell suspension enzyme-linked immunosorbent assay to demonstrate and measure antibody binding

A simple cell suspension enzyme-linked immunosorbent assay system, which can be used to test the reactivity of antibodies with cells, is described. Such a system is of particular use with cells that are difficult to fix to microtitre plates or when measuring antigens which are labile to fixation techniques.     

Fixed closest distance of approach dipole potential mapping: An effective alternative to the monopole isopotential approach

It has been shown that an easier procedure to the study of molecular electrostatic potentials than the prevalent monopole isopotential approach is to use a dipole as the mapping device: allow the dipole to move on a geometrical surface enclosing the molecule such that the distance between the surface and the nearest atoms of the molecule (closest distance of approach) is fixed, say at 2 Å, calculate the electrostatic interaction energy between the molecule and the dipole at different points of the surface, and let the dipole orient itself along the minimum energy direction and treat these minimum energies as the desired electrostatic potentials. Thus the favorable binding sites of a given molecule for other species along with their relative orientations can be obtained. This procedure has been applied to the nucleic acid bases using the necessary input data from two independent sources. Thus success of the procedure in predicting important features of molecular electrostatic potentials has been demonstrated.     

表面等离子体激元共振光化学传感器的研究

自行设计并组装了一套全波长表面等离子体激元共振光化学传感器实验装置;研究了以银膜为基底的传感器的稳定性、可逆性及干扰情况;在575nm波长处,测定乙醇的灵敏度为4.6×10^-4折射率单位,RSD≤1.4%,定量测定范围为0.5%～70%.分析了一些实际样品,结果与标准方法的测定值相吻合.     

Preparation and Photophysical and Photoelectrochemical Properties of a Covalently Fixed Porphyrin–Chemically Converted Graphene Composite

Chemically converted graphene (CCG) covalently linked with porphyrins has been prepared by a Suzuki coupling reaction between iodophenyl‐functionalized CCG and porphyrin boronic ester. The covalently linked CCG–porphyrin composite was designed to possess a short, rigid phenylene spacer between the porphyrin and the CCG. The composite material formed stable dispersions in DMF and the structure was characterized by spectroscopic, thermal, and microscopic measurements. In steady‐state photoluminescence spectra, the emission from the porphyrin linked to the CCG was quenched strongly relative to that of the porphyrin reference. Fluorescence lifetime and femtosecond transient absorption measurements of the porphyrin‐linked CCG revealed a short‐lived porphyrin singlet excited state (38 ps) without yielding the porphyrin radical cation, thereby substantiating the occurrence of energy transfer from the porphyrin excited state to the CCG and subsequent rapid decay of the CCG excited state to the ground state. Consistently, the photocurrent action spectrum of a photoelectrochemical device with a SnO₂ electrode coated with the porphyrin‐linked CCG exhibited no photocurrent response from the porphyrin absorption. The results obtained here provide deep insight into the interaction between graphenes and π‐conjugated systems in the excited and ground states.     

Driving force for hydraulic and pervaporative transport in homogeneous membranes

Experiments were designed to demonstrate that the chemical potential gradient required for liquid transport through swollen network polymer membranes manifests itself as a concentration gradient and that the rate of transport is independent of how this gradient is established. The fluxes of various liquids through a crosslinked rubber membrane were measured in hydraulic and pervaporation modes of permeation. The pressure applied downstream in the latter act simply to fix the activity of the liquid in the downstream membrane surface. The experiments show the flux is a unique function of this activity, and it does not matter how it is established. Sorption data were used to convert these results into a plot of flux versus concentration differential across the membrane which was analyzed by Fick's law using a model for the concentration dependence of the diffusion coefficient. Measured ceiling fluxes for pervaporation for a number of liquids were found to be the same as those estimated from hydraulic permeation data. A simple mathematical representation for an ideal system is used as a pedagogical device to demonstrate the conclusions.     

Activation and functionalization of molecular nitrogen by metal complexes

Molecular nitrogen is intrinsically unreactive, so much so that it has confounded chemists for decades in attempts to functionalize this abundant diatomic molecule. While biological systems and industrial processes can fix nitrogen to form ammonia, the challenge is to discover a process that involves a homogeneous catalyst that can utilize N(2) as a feedstock to generate higher value organonitrogen materials. In this review, the activation of molecular nitrogen by transition metal complexes is reviewed with the view to present new kinds of transformations for coordinated dinitrogen. Moreover, some reaction types that are as yet unknown are outlined to try and stimulate further research in this area.     

应用于活性负离子聚合的高真空实验技术研究与改进

高真空实验技术在装置设计、加工制作以及操作实施方面需要实验人员具备熟练的玻璃烧接技术;细颈的制作及反应装置的退火处理是较为关键的操作;考虑到聚合反应装置的体积较大,对火封操作进行了设计改进,使其能够应用于位置固定的装置;对操作中频繁采用的易碎封口安瓿进行了设计改进,使实验操作更为便利;针对已封闭装置预留的细颈部位,设计...     

Continuous-time random walk models of DNA electrophoresis in a post array: part II. Mobility and sources of band broadening

Using the two-state, continuous-time random walk model, we develop expressions for the mobility and the plate height during DNA electrophoresis in an ordered post array that delineate the contributions due to (i) the random distance between collisions and (ii) the random duration of a collision. These contributions are expressed in terms of the means and variances of the underlying stochastic processes, which we evaluate from a large ensemble of Brownian dynamics simulations performed using different electric fields and molecular weights in a hexagonal array of 1 μm posts with a 3 μm center-to-center distance. If we fix the molecular weight, we find that the collision frequency governs the mobility. On the contrary, the average collision duration is the most important factor for predicting the mobility as a function of DNA size at constant Péclet number. The plate height is reasonably well described by a single post rope-over-pulley model, provided that the extension of the molecule is small. Our results only account for dispersion inside the post array and thus represent a theoretical lower bound on the plate height in an actual device.