HPTLC Method for Analysis of Withaferin-A in Ashwagandha (Withania somnifera)

JPC – Journal of Planar Chromatography – Modern TLC - A densitometric high-performance thin layer chromatographic (HPTLC) method has been established for analysis of withaferin-A in...     

A Rapid Densitometric Method for the Quantification of Luteolin in Medicinal Plants Using HPTLC

Luteolin, a flavonoid, is reported to occur widely in many medicinal plants. It has been shown to have important biological activities. We report sensitive HPTLC method for the quantification of luteolin from plant material. The method was validated for precision, repeatability and accuracy. The method was found to be precise with RSDs for intraday in the range of 0.77–1.29% and inter–day in the range of 1.02–2.08%. Instrumental precision and repeatability of the method were found to be 0.39 and 0.57 (%CV). Accuracy of the method was checked by recovery study conducted at two different levels and the average percentage recovery was found to be 100.92%. The method was used for quantification of luteolin in three important herbal drugs viz. fruit of Cuminum cyminum, whole plant of Bacopa monnieri, flower of Achillea millefolium. The proposed HPTLC method for the quantification of luteolin was found to be simple, precise, specific, sensitive and accurate and can be used for quality control of raw materials.Revised: 1 October 2003 and 18 February 2004     

Validated HPTLC Method for Quantification of Vitamin D3 in Fish Oil

JPC – Journal of Planar Chromatography – Modern TLC - A selective high-performance thin-layer chromatographic (HPTLC) method for the quantification of vitamin D3 was developed and...     

Development and Validation of HPTLC Densitometric Method for Identification and Quantification of Geraniol in Palmarosa Oil

JPC – Journal of Planar Chromatography – Modern TLC - India is the main producer of palmarosa oil obtained from rosha grass (Cymbopogan martini var. motia) of family Graminae. The...     

Quantification of Major Bioactive Withanolides in Withania somnifera (Ashwagandha) Roots by HPTLC for Rapid Validation of Ayurvedic Products

JPC – Journal of Planar Chromatography – Modern TLC - The high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous quantification of withaferine...     

Densitometric HPTLC Method for Simultaneous Quantification of Sennosides A and B and Gallic Acid in a Pharmaceutical Dosage Form

JPC – Journal of Planar Chromatography – Modern TLC - A densitometric HPTLC method has been established for simultaneous quantification of sennosides A and B and gallic acid in a...     

A Validated,Stability-Indicating HPTLC Method for Analysis of Doxofylline

JPC – Journal of Planar Chromatography – Modern TLC - A simple, sensitive, selective, precise, and stability-indicating high-performance thin-layer chromatographic method for analysis...     

Densitometric HPTLC Method for Qualitative and Quantitative Analysis of Piperitone

JPC – Journal of Planar Chromatography – Modern TLC -     

Quantification of the Two Pharmacologically Important Triterpenes in Various Plant Parts of Diospyros sylvatica Using Validated High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - In view of high pharmacological significance and urgent need to identify high-yielding plant sources of betulin (1) and betulinic...     

Isolation and Densitometric HPTLC Method for Quantification of Belleric Acid in the Fruit Pericarp of Terminalia bellerica and its Formulations

JPC – Journal of Planar Chromatography – Modern TLC - For the first time a simple, sensitive, rapid, precise and accurate HPTLC method has been established for quantification of...     

UV Densitometric HPTLC Method for Analysis of Nitrazepam in Pharmaceutical Formulations

JPC – Journal of Planar Chromatography – Modern TLC - A UV densitometric high-performance thin-layer chromatographic (HPTLC) method for analysis of nitrazepam in pharmaceutical...     

Application of Videodensitometric and Classical Densitometric HPTLC for Quantification of Fluoroquinolone Derivatives in Pure and Tablet Forms

JPC – Journal of Planar Chromatography – Modern TLC - A new, simple, and rapid high-performance thin layer chromato-graphic method coupled with densitometry has been developed and...     

Improved and Validated HPTLC Method for Quantification of Oenothein B and its Use for Analysis of Epilobium angustifolium L.

JPC – Journal of Planar Chromatography – Modern TLC - A selective and simplest possible high-performance TLC (HPTLC) method for quantification of oenothein B on the basis of the free...     

A Validated Stability-Indicating HPTLC Method for the Estimation of Gemcitabine HCI in its Dosage Form

JPC – Journal of Planar Chromatography – Modern TLC - Gemcitabine belongs to a class of drugs known as antimetabolites (pyrimidine analog) used in various carcinomas, such as pancreatic...     

A Validated HPTLC Method for Simultaneous Analysis of Eugenol and Piperine in a Siddha Formulation

JPC – Journal of Planar Chromatography – Modern TLC - A high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of eugenol and piperine...     

A Validated HPLC Method for Quantification and Optimization of Furocoumarins in Different Extracts of Fruits of Heracleum candicans

Heracleum candicans Wall. ex DC (Apiaceae) finds use as an aphrodisiac, nerve tonic and also in the treatment of skin diseases in the Indian system of medicines and is reported to be rich in furocoumarins which are extensively used in pharmaceutical industry for their photosensitizing activity on human skin. Thus a HPLC–PDA method has been developed for the selection of suitable solvent for extraction and quantitative estimation of these furocoumarins viz. heraclenol, heraclenin, bergapten and psoralen in fruits of H. candicans. The results indicate that heraclenol is found to be maximum in 30% aqueous methanolic extract (0.108%) while the maximum concentration of heraclenin (anti-inflammatory principle) and bergapten (melanogenesis stimulation activity) was found in the pure methanolic extract (0.386 and 0.281%, respectively). However psoralen (anti-psoriatric principle) was found in very low concentration in all the extracts. Further validation of the method was done in view to demonstrate its selectivity, linearity, precision, accuracy and robustness. The method thus developed can be applied in quality evaluation of H. candicans.     

Densitometric HPTLC Method for Analysis of Oleanolic Acid in Achyranthes aspera L.

JPC – Journal of Planar Chromatography – Modern TLC - Adensitometric HPTLC method has been established for analysis in alcohol extracts of the seeds and leaves of Achyranthes aspera L....     

TLC Densitometric Method for the Quantification of Eugenol and Gallic Acid in Clove

Eugenol and gallic acid are reported from the flower buds of Syzygium aromaticum (L.) Merr. & Perry (clove). Both the compounds have been shown to give interesting biological activities and hence serve as biomarkers. We report a simple TLC densitometric method for the quantification of eugenol and gallic acid in clove. The method was validated for precision, repeatability and accuracy. The method was found to be precise with RSD of 0.61 and 1.3 (intraday) and 0.96 and 0.24 (interday) for different concentrations of eugenol and gallic acid respectively. Instrumental precision was 0.24 and 0.21 (% CV) for eugenol and gallic acid respectively. Accuracy of the method was checked by conducting recovery study at two different levels for eugenol and gallic acid and the average percentage recoveries were found to be 99.79% and 97.90% respectively. The contents of eugenol and gallic acid in different samples of clove, as estimated by the proposed method, were found to be in the range of 12.9–14.6% and 0.31–0.61% respectively. The proposed HPTLC method for the estimation gallic acid and eugenol was found to be simple, precise, specific, sensitive and accurate and can be used for routine quality control of clove.     

Stability-Indicating Densitometric HPTLC Method for Qualitative and Quantitative Analysis of Hydroquinone in Commercial Whitening Creams

JPC – Journal of Planar Chromatography – Modern TLC - A densitometric high-performance thin-layer chromatographic (HPTLC) method for analysis of hydroquinone has been developed and...     

New Rapid Validated HPTLC Method for the Determination of Lycorine in Amaryllidaceae Plants Extracts

A rapid, accurate, specific, repeatable and robust HPTLC method for the determination of lycorine in different Amaryllidaceae plant extracts is presented in this work. No article related to the HPTLC determination of lycorine in plant extracts has been reported in literature. Lycorine, a common alkaloid of family Amaryllidaceae, moreover, there have been some recent reports which reveal the interaction of lycorine with DNA and tRNA. It has, therefore, been to the interest of phytochemists to determine the content of this alkaloid in Amaryllidaceaous plants.