Cell culture device using spatial light modulator

Spatial light modulator is introduced for cell culturing and related illumination experiment. Two kinds of designs were used. The first type put the cell along with the bio-medium directly on top of the analyzer of the microdisplay and set a cover glass on it to retain the medium environment, which turned the microdisplay into a bio-container. The second type introduced an optical lens system placed below the spatial light modulator to focus the light spots on specific position. Details of the advantages and drawbacks for the two different approaches are discussed, and the human melanocyte cell (HMC) is introduced to prove the feasibility of the concept. Results indicate that the second type is much more suitable than the first for precision required application.     

Modulation transfer function of a liquid crystal spatial light modulator

The modulation transfer function (MTF) of a pixelated liquid crystal spatial light modulator (LC-SLM) has been derived as a function of the fill factor. Based on the formula, we have investigated the dependence of the MTF on the orientations and spatial frequency of the input patterns. Furthermore, we have proposed and demonstrated a method for improving the MTF of a LC-SLM.     

Optical phase-based beamformer using MZM SSB modulation combined with crystal polarization optics and a spatial light modulator

The performance of a widely tunable phase-based beamformer for phased array antennas using a new technique to cross-polarized the carrier and the sideband, in order to allow the phase control by means of a spatial light modulator, is experimentally demonstrated. The technique relies on the combination of single sideband amplitude modulation (SSB) using a Mach-Zehnder modulator (MZM) and birefringence (to cross-polarized the carrier and the sideband). The architecture has the potential of controlling multiple independent beams simultaneously. The beamformer feeds an eight elements array showing an insertion loss and a reset speed of around 12 dB and 70 ms, respectively. Far-field antenna patterns between 7.5 GHz and 8.5 GHz for nine elevation angles within a range of ±20° have been measured showing beam steering capability, amplitude distribution weighting as well as multibeam operation.     

High resolution digital holography

In digital Fresnel holography, present specifications of charge-coupled device cameras require the incident beams to be quasi-parallel. That implies large speckle grain size and low lateral resolution in reconstructed images. Better lateral resolutions are demonstrated in our work, down to 8 μm, allowing the observation of sub-millimetre objects by digital holography. The experimental set-up built samples the incident light distribution with a definition of 500 pixels mm⁻¹. The maximum acceptable angle is then widened and the hologram recording and reconstruction distances are drastically reduced, as well as the speckle size. Interferometric holography was implemented for the measurement of the deformation of a sub-millimetre silicon cantilever and results in phase-contrasts imaging are also reported. The design of dedicated complementary metal-oxide-semiconductor active pixel sensor cameras is also discussed.     

Performance evaluation of fiber Bragg grating sensors by digital holographic technique, strain gauge measurement

In view of the growing interest for non-destructive tests of materials, geodynamical monitoring and in general remote sensing, there is a great effort to bring practical optical sensors from research labs to industrial and environmental applications. In this paper, we employ digital holographic technique as an efficient tool for evaluating the strain measurement capability of fiber Bragg gratings (FBG). A cantilever beam has been employed as a test structure under loading test. The strain measurements results obtained by fiber-based sensors have been compared to those obtained by using full-field digital holographic technique and point-wise strain gauge sensors glued on the same cantilever beam. A simple theoretical model is also presented to interpret and compare the experimental results coming from different techniques.     

Reduction of speckle noise in digital holography by using digital image processing

A fundamental problem in optical and digital holography is the presence of speckle noise in the reconstruction process. Many approaches have been carried out in order to overcome such a problem ranging from modifying the spatial coherence of the illumination (optical techniques) to image processing techniques (digital techniques). This work shows the merged use of digital image processing techniques in order to reduce the speckle noise in digital reconstruction of optically recorded Fresnel's holograms. The proposed filtering techniques are illustrated with experimental results.     

利用相位模板实现数字全息超分辨成像

为了简化数字全息超分辨记录系统,分别在其物光和参考光部分引入一块相位模板,以获得垂直和倾斜方向照明物体的光束和具有不同载波频率的参考光束.当这些具有不同照射方向的光透过物体后,可以使CCD在位置固定的情况下记录到携带低频和高频信息的物体衍射场,不同载波频率的参考光则保证了高频和低频信息在复合全息图的频谱面上能够相互分离.实验结果证明,通过将记录到的物体高频和低频信息合成,可以获得超出系统衍射极限分辨率的再现像.     

Real-time optical reconstruction of the diffused 3D object using phase information calculated by Continuous Wavelet Transform

We propose the real time optical reconstruction of a three-dimensional (3D) object from a digitally recorded hologram. Phase distribution of the recorded hologram is numerically calculated by 1-dimensional Continuous Wavelet Transform (1D-CWT) for digital reconstruction with phase only information. Also, the phase distribution of 1D-CWT transferred to the spatial light modulator (SLM) is used to obtain optically reconstructed image. It is observed that an efficient real-time analysis can be achieved, if phase of 1D-CWT is used. In the same time, optically reconstructed 3D objects obtained by only phase information are about three times brighter than bleached hologram intensity's. So the minimum power loss can be obtained. Numerical and experimental results are presented.     

Variable shape or variable diameter flattop beam tailored by using an adaptive weight FFT-based iterative algorithm and a phase-only liquid crystal spatial light modulator

The variable shape or variable diameter flattop beam shaping technique by using an adaptive weight FFT-based iterative algorithm (AWFFT-IA) and a phase-only liquid crystal spatial light modulator (PO-LCSLM) is demonstrated. The algorithm is used to design the phase distribution for tailoring central symmetric laser beam into the desired shape or diameter flattop beam, the PO-LCSLM serves as dynamically programmable phase filters, which depend on the designed phase distributions, to retard the incident wavefront as we would expect. The experimental results show that the technique can realize variable shape or variable diameter flattop beam shaping.     

Contouring of diffused objects by using digital holography

A simple method for contouring of diffused objects by using lensless Fourier transform digital holography (LFTDH) and dual-index immersion method is presented. Depth contour interval up to minimum of 0.12 mm could be achieved without making the interference phase fringes over crowded.     

Nonlinear image self-filtering with liquid crystal spatial light modulator

Nonlinear spatial filtering is realized due to the electro-optical effect in spatial light modulators (SLMs). Optical methods with nonlinear filtering are suggested for various optical applications such as optical inspection of photolithography masks, wavefront sensor, etc. A fast optical response of the SLM allows optical inspection with a video (or faster) rate. The optical system proposed is tolerant to the vibrations and temperature variations because of the dynamic properties of the SLM.     

Lensless digital holography with short-coherence light source for three-dimensional surface contouring of reflecting micro-object

In this paper, a new lensless digital holography system with short-coherence light source is reported for recording three-dimensional surface contouring of reflecting micro-objects. In the experiment, each of the layers on the inwall of a conical pore is respectively recorded by changing the path length of the object beam, instead of changing that of the reference beam, which can reduce the recording complexity and errors. In addition, the least-square-polynomial-fitting is used for the first time to carry out three-dimensional reconstruction with a series of two dimensional intensity images of a micro-object, which can be used not only to reduce obviously the complication of the three-dimensional reconstruction, but also to carry out three-dimensional reconstruction of a micro-object with strong laser speckle noise, of which the phase images can not be obtained from the conventional phase unwrapping process.     

Information reduction using lensless Fourier transform digital composite holography

An optical hologram contains substantially more information than necessary for some specific applications. Practical methodology of handling huge information contents in these holograms for such applications is cumbersome. In this paper, a method of information reduction, which aims at efficient storage and transmission of holograms, is investigated using digital composite holography in lensless Fourier transform configuration. The maximum information reduction factor obtained in our experiment is 105. The advantages/disadvantages of this method over an earlier method used in optical holography [Lin LH. A method of hologram information reduction by spatial frequency sampling. Appl Opt 1968;7:545] are also discussed.     

Phase measurements of a twisted nematic liquid crystal spatial light modulator with a common-path interferometer

We present a novel and high accuracy (±1°) technique for measuring the difference in phase delay between the positive and negative eigenvectors of a twisted nematic liquid crystal spatial light modulator (LCSLM) as a function of applied voltage. We show a new architecture for a common-path interferometer where we simultaneously generate both the positive and negative eigenvectors of the LCSLM. We use an electro-optic light modulator to provide a time varying phase shift between the two eigenvectors and measure the phase delay difference between the two eigenvectors. We compare the phase delay values with those obtained using a Mach–Zehnder interferometer and find good agreement.     

A novel three-dimensional holographic display system based on LC-R2500 spatial light modulator

A novel holographic display system is proposed in this paper. The system takes LC-R2500, a kind of reflective liquid crystal spatial light modulator, (LC-SLM) as the core display unit, which can meet the requirement of real-time reconstruction of three-dimensional (3D) objects from holograms in free space. The relationship between hologram recording and image reconstruction is discussed, and the parameters associated with the magnification of reconstructed image over original object are determined. Experimental results of holographic display using the system are also given in the end.     

Compatibility of temporal multiplexed spatial light modulator with optical image processing

The tendency today is to replace high-dynamic light modulators by high-speed binary ones (of which the micromirror is the best example). This kind of spatial light modulators (SLM) fulfils all the present needs in displays. They are used in optical communications as binary systems and also in display applications (video projectors) with temporal multiplexing, in order to generate greyscale or colour images. In optical processing, and in the majority of coherent applications, temporal dithering introduces some distortions. In this paper, this point is studied with simulations. We point out that temporal multiplexing cannot be used in the Fourier plane. In the imaging plane, the distortion is weak if the filter has a positive impulse response.     

Experimental validation of closed-loop adaptive optics based on a self-referencing interferometer wavefront sensor and a liquid-crystal spatial light modulator

A closed-loop adaptive optics system based on a self-referencing interferometer wavefront sensor (SRI WFS) using phase-shifting point-diffraction technique and an electrically addressed phase-only liquid-crystal spatial light modulator (LC SLM) is built and validated experimentally. The wavefront aberration of incoming beam is directly measured by analyzing four frames phase-shifted interferograms captured by a single CCD camera in two camera shots, and then by loading the conjugate function onto the SLM the wavefront aberration is corrected. The proposed scheme does not rely on any complicated control algorithm or wavefront-reconstruction algorithm and can achieve high-resolution and high-accuracy correction. Closed-loop correction results of single order Zernike aberrations and a Kolmogorov turbulence phase screen show that considerable improvements in the Strehl ratio (of greater than 0.94) is achieved.     

Three-dimensional dynamic optical manipulation by combining a diffractive optical element and a spatial light modulator

Since the introduction of computer-controlled spatial light modulators (SLMs), holographic optical tweezers have become an important tool for dynamic parallel optical manipulation. In this paper we clarify the usefulness of a new configuration for optical trapping that creates light patterns using the combination of a diffractive optical element (DOE) and an SLM. This configuration not only enables the use of the higher part of the SLM’s diffraction efficiency curve, because a simple hologram can be chosen for the SLM, but also achieves three-dimensional dynamic optical manipulation over a large spatial range. By switching blaze-like holograms displayed on the SLM, we demonstrated simultaneous transportation of three 6-μm-diameter polystyrene beads over a range of 90 μm in the vertical direction and 37.5 μm in the horizontal direction. Compared with the same manipulation executed using only the SLM, the range of this method is extended four-fold in the vertical direction and three-fold in the horizontal direction.     

Analysis of influence of black matrix effect in spatial light modulator on optical correlation detection

Motion detection with a joint transform optical correlator is based on the maximally correlated bright spots in a correlation image; therefore, the quality of a correlation image greatly influences optical correlation detection. As the core component of a joint transform optical correlator, a spatial light modulator has an opaque part (black matrix structure) that is an important factor that needs to be considered. In this paper, we first analyzed the effect of the fill factor on the light energy distribution in the image plane according to the mechanism of the spatial light modulator using a multiple-subpixel matrix to simulate a single pixel, while zeroing some subpixels to simulate the black matrix part in the single pixel and employing computer software to simulate the joint transform optical correlator to obtain the simulated correlation image result with a black matrix effect. In addition, we built an experimental setup to obtain an actually photographed correlation image, which was well consistent with the simulated result.     

Design of a device with wavelength multiplexing and routing functions using dynamic holography on SLMs

This work describes the theory and design of a generic multipurpose device that can operate as a tunable wavelength filter, wavelength multiplexer and wavelength router. This device could be especially useful in optical network applications based in both coarse and dense wavelength division multiplexing technology. The enabling component is a ferroelectric liquid crystal spatial light modulator (SLM) where dynamic holograms are implemented in real time. As a consequence, the device will be able to carry out different functions according to the hologram recorded onto the SLM. The great advantage of this device is a polarization insensitivity operation, allowing low crosstalk and simple handling.