Estimation of Bluish Appearance of Veins in Skin Tissue Using Spectrocolorimetry

The bluish appearance of veins in the skin tissue was experimentally investigated by in vivo and in vitro spectrophotometric measurements. Color of the skin surface including the veins was quantitatively evaluated by employing color perception on the basis of CIEXYZ and CIELAB colorimetric systems. For in vivo measurements, the bluish appearance of veins was successfully estimated using a dominant wavelength, excitation purity, and color difference. Results of in vitro experiments using a simple skin tissue model demonstrated that the degree of bluish appearance depends on the vessel depth, diameter, and the blood content in the surrounding medium.     

Three Dimensionality of the Recognized Visual Space of Illumination Proved by Hidden Illumination

We hypothesized that the recognized visual space of illumination (RVSI) was constructed in our brain when we grasped the state of illumination of a space. The importance about the RVSI is that it is three dimensional and is valid not only at the surfaces of the existing objects in the space, but also for the entire portion in the space where no objects exist. With this property of RVSI we are able to predict the appearance of an object surface in terms of lightness as well as of color when the object shifts from one place to the other in the space. The three dimensionality of the RVSI is proved by giving a hidden illumination within a space and by asking a subject to judge the lightness or color of a test patch placed in the area of the hidden illumination. In spite of the additional light on the test patch the subject did not recognize that the light was added but simply felt that the surface was made of a higher lightness or colored by transferring the light into an increase of the reflectance factor of the test patch. The results can be interpreted if we assume that a same RVSI exists throughout the entire space including the area of the hidden illumination.     

基于图像亮度特性统计的水下距离选通成像自动增益控制方法

对水下激光距离选通成像质量与系统增益的关系进行了分析,提出了一种基于图像亮度特性统计的普适性自动增益控制方案。该方案通过设定不同灰度统计阈值,甄别目标图像的亮度特性,并以此作为控制系统增益的主要依据。在对水下大动态范围照度目标或慢速运动目标等进行成像的特殊场合,该方案也具备较强的适用性,能实时反馈控制系统增益,使成像质量优化。     

Phenomena of Apparent Lightness Interpreted by the Recognized Visual Space of Illumination

Many experimental results have been reported which demonstrated deviation of the apparent lightness from the calculated lightness based on spectral reflectance, and these have caused debate among researchers as to the models to explain them. The judgement of lightness of objects that we see in the outside world is one of the most important tasks in our daily life. We proposed the recognized visual space of illumination, RVSI, as a three dimensional recognition constructed in the brain for the outside world, and showed that the apparent lightness was determined in relation to the size of the RVSI. In the present paper the concept was applied to various results of lightness experiments such as the White effect and simultaneous contrast, and based on the proposition that an observer first builds a three dimensional RVSI from a two dimensional pattern and the lightness of a test patch was judged in relation to the size of this RVSI, the results were then globally and nicely explained. A new demonstration of a pattern was proposed to give different apparent lightness for patches with the same physical lightness to strengthen the proposition. The importance of distinguishing between a test patch and a surrounding field was emphasized when one does a lightness experiment and interprets the results.     

Detection of Label Area by Comb-Shaped Illumination

We have developed a non-contacting technique to detect small steps on a surface such as label edges on a letter by using a spatially modulated illumination. Steps can be detected correctly with this new technique even if there are printed patterns on the same surface. Thus the technique is effective for determining label areas that are often seen on pieces of direct mail.This paper was originally presented at the 2nd International Conference on Optical Design and Fabrication, ODF2000 which was held on November 15–17, at the International Conference Center, Tokyo, Waseda University, Japan.     

分波段照明法实现单透镜大景深成像

基于透镜色散原理,提出了运用分波段照明法扩大单透镜成像景深的方法.理论推导出物距和照明光波长之间存在正相关关系,据此选择相应波段的照明光源,通过照明光学设计,使不同物距的物体均能清晰成像在同一像面上.ZEMAX软件模拟结果与理论分析吻合.采用自行设计的图像采集系统,只用一个35mm焦距普通双凸透镜进行实验测试.人眼主观和专业软件测试结果均表明:采用分波段照明方式比用白光照明景深扩大81.8%,分辨率最大提升200.3%;比只用单色光照明景深扩大了93.6%,分辨率最大提升189.7%.     

Color Property of the Recognized Visual Space of Illumination Controlled by Interior Color as the Initial Visual Information

It was shown that the color property of the recognized visual space of illumination, RVSI was controlled by changing the initial visual information by arranging objects in the room all shifting toward orange direction. We constructed two miniature rooms, D and I, both illuminated by the same daylight type fluorescent lamps but arranged with furniture of different color, those in room I shifting toward color as if they were illuminated by an incandescent lamp. Subjects felt as if room I were illuminated by an incandescent lamp. A test patch was placed midair in each room and its color was judged. When the test patches were placed in room I their colors were all perceived to be shifted toward greenish blue compared to those of test patches placed in room D, in spite of having the same illumination. The results imply that the apparent color of an object is determined not by its chromaticity, but in relation to the color property of the RVSI of the room where the object is observed.     

Color Appearance Explained,Predicted and Confirmed by the Concept of Recognized Visual Space of Illumination

Ten years have passed since we proposed a new concept called recognized visual space of illumination (RVSI). The central idea of the concept assumes that our brain first recognizes how a space is illuminated and then judges colors of anything seen in the space in relation to the RVSI constructed for the space. In another expression we say that the space is recognized first and the color perception next. In this paper some of our experiments that proved the appropriateness of the concept will be introduced. When a white paper was seen through a colored filter we could perceive the paper as white at the same instant we recognized a space through and beyond the filter. When an achromatic patch independent from the room illumination was observed under colored illumination its appearance immediately changed to that roughly opponent to the illumination color. When two gray patches of the same lightness were drawn in a picture of a white grating on a black background on a way that one appeared to locate on this side of the grating and the other in the other side, the former appeared brighter. These all confirmed the predictions based on the RVSI concept.     

Assessment of tissue viability by polarization spectroscopy

A new and versatile method for tissue viability imaging based on polarization spectroscopy of blood in superficial tissue structures such as the skin is presented in this paper. Linearly polarized light in the visible wavelength region is partly reflected directly by the skin surface and partly diffusely backscattered from the dermal tissue matrix. Most of the directly reflected light preserves its polarization state while the light returning from the deeper tissue layers is depolarized. By the use of a polarization filter positioned in front of a sensitive CCD-array, the light directly reflected from the tissue surface is blocked, while the depolarized light returning from the deeper tissue layers reaches the detector array. By separating the colour planes of the detected image, spectroscopic information about the amount of red blood cells (RBCs) in the microvascular network of the tissue under investigation can be derived. A theory that utilizes the differences in light absorption of RBCs and bloodless tissue in the red and green wavelength region forms the basis of an algorithm for displaying a colour coded map of the RBC distribution in a tissue. Using a fluid model, a linear relationship (cc. = 0.99) between RBC concentration and the output signal was demonstrated within the physiological range 0–4%. In-vivo evaluation using transepidermal application of acetylcholine by the way of iontophoresis displayed the heterogeneity pattern of the vasodilatation produced by the vasoactive agent. Applications of this novel technology are likely to be found in drug and skin care product development as well as in the assessment of skin irritation and tissue repair processes and even ultimately in a clinic case situation.     

Medical application of diffraction enhanced imaging in mouse liver blood vessels

Neovascularization is correlative with many processes of diseases, especially for tumor growth, invasion, and metastasis. What is more, these tumor microvessels are totally different from normal vessels in morphology. Therefore, observation of the morphologic distribution of microvessels is one of the key points for many researchers in the field. Using diffraction enhanced imaging (DEI), we observed the mirocvessles with diameter of about 40 μm in mouse liver. Moreover, the refraction image obtained from DEI shows higher image contrast and exhibits potential use for medical applications.

     

Medical application of diffraction enhanced imaging in mouse liver blood vessels

Neovascularization is correlative with many processes of diseases, especially for tumor growth, invasion, and metastasis. What is more, these tumor microvessels are totally different from normal vessels in morphology. Therefore, observation of the morphologic distribution of microvessels is one of the key points for many researchers in the field. Using diffraction enhanced imaging （DEI）, we observed the mirocvessles with diameter of about 40 μm in mouse liver. Moreover, the refraction image obtained from DEI shows higher image contrast and exhibits potential use for medical applications.     

Color Appearance of a Patch Explained by RVSI for the Conditions of Various Colors of Room Illumination and of Various Luminance Levels of the Patch

Whenever we enter a space illuminated differently from a previous space whether in color or in illuminance, we can quickly adapt to the new atmosphere and can again perceive white for the originally white object; this is known as color constancy. This phenomenon is explained by rotation of the recognition axis of the recognized visual space of illumination (RVSI) toward the illumination color. The explanation then predicts that the color appearance of a test patch changes radically toward the opposite direction from the color of illumination when the physical property of the test patch is kept unchanged at a neutral white. This prediction was confirmed by Experiment 1, where eight different colors of illumination were employed. The test patch appeared very vivid in color and shifted toward the opposite direction from the color of the illumination. In RVSI theory the light source color mode is explained by the release of the test patch from the restriction of RVSI. The release can be achieved by increasing the luminance of the test patch and the color appearance of the patch should then return to its own color as it is no longer controlled by RVSI. In Experiment 2 these predictions were investigated by increasing the luminance of the test patch to a much higher level than that of the objects in the lit room fixed at an illuminance of about 1001x. The color appearance of the test patch indeed became the light source color and returned to the original neutral white. Emphasis was given in the course of the experiments that the subjects were observing the test patch presented in a real 3D space where the subjects also stayed inside so that they could properly construct RVSI for the space.     

FT‐Raman spectroscopic study of thoracic aortic wall subjected to uniaxial stress

The combination of Fourier transform‐Raman spectroscopy and uniaxial tensile tests (in MTS Synergie 100 testing machine) was used to investigate microstructural changes in the secondary protein structure of the aortic wall under different levels of stress. The spectroscopic analysis clearly shows differing tension thresholds for material excised in two directions: circumferential and longitudinal. This is confirmed by the results of macroscopic mechanical analyses. The application of strain does not lead to any noticeable change in the bandwidths of the Raman bands. The stress‐controlled Raman band analysis shows that the modes at 938 cm⁻¹ assigned as C_α C of the α‐helix, 1660 cm⁻¹ amide I (the unordered structure of elastin) and 1668 cm⁻¹ amide I (the collagen triple helix) undergo wavenumber shifting, but the bands at 1004 cm⁻¹ assigned to the phenyl ring breathing mode and 2940 cm⁻¹ to the ν (CH₃) and ν (CH₂) modes are not affected during the elastic behaviour. A clear correlation between Raman band shifting and the level of mechanical stress has been established. Elastin alone participates in the transmission of low stresses in the circumferential direction, whereas both elastin and collagen take part in the transmission of physiological and higher stresses. Copyright © 2009 John Wiley & Sons, Ltd.     

Shape Measurement from Endoscopic Images: Determination of Dimensional Scale Factor by a Photometric Method

We propose a new method to measure surface topography using a monocular endoscope. The surface structure is obtained up to a scale factor from an image sequence taken through a conventional CCD endoscope. The scale factor is determined by a photometric method, and the actual size of the object can be determined. The principle and experimental results are presented.     

隐失驻波全内反射荧光显微术的数值计算

从理论上详细计算了在全内反射的条件下，两束入射光产生的隐失干涉场(即隐失驻波)的强度分布，并分析了其不同于传统传播波干涉场的特点。同时使用数值模拟证明了利用隐失干涉场，即隐失驻波的激发方式，可以提高系统的分辨力，在横向实现超经典分辨的荧光成像。具体的分析表明，两束光以相等的角度入射，同时振幅相等，偏振态相同，所形成干涉条纹的反衬度最高，此时成像系统的有效点扩展函数最优化；入射介质1的折射率越大，隐失干涉场的空间周期越短(空间频率越高)，其对应的调制点扩展函数中心瓣的半峰全宽越小，可能分辨更小的物体，但同时旁瓣的强度也增强，最终成像的分辨力受两者的共同制约。     

乳腺组织的拉曼光谱线性模型研究

本研究甄选10个代表乳腺组织拉曼活性成分的基谱,在国内首次构建一种乳腺组织拉曼谱的线性回归模型。用2 000多个正常和非正常乳腺组织拉曼谱对该模型进行统计检验,模型显著性F检验的置信度全部为1,多元决定系数的平均值为0.95,表明线性模型假设成立、拟合效果良好。10个基谱代表脂肪、细胞质、细胞间质、DNA、血液、β-胡萝卜素、胆固醇等的拉曼谱,基谱的归一化拟合系数间接反映出这些成分的相对含量。用该模型拟合正常和肿瘤乳腺组织的拉曼宏观谱,病变前后细胞质和DNA的拟合系数增大、脂肪拟合系数减小,这反映出它们相对含量的增减,与已知的病理学结果一致。该研究有助于理解乳腺肿瘤组织的生化变化,并可能为分析该变化提供了有效手段。     

HPLC-UV对人组织激态释放酶的测定方法研究

HPLC-UV结合的方法是一种灵敏度高、分辨率高的分离检测方法。本文利用HPLC-UV测定了人组织激肽释放酶的最大吸收波长,结果表明人组织激肽释放酶在200nm具有最大紫外吸收;并对昆虫细胞培养液中的人组织激肽释放酶进行定性检测,具有灵敏度高,所用的分析时间短等特点。     

Blood Flow Analyses of Skin Tissue Under the Sacrum Using Laser Speckle Flowgraphy

A new version of the Laser Speckle Flowgraphy was developed to study the dependence of the blood flow of the skin tissue under the sacrum and the supporting pressure against the sacrum. The measuring unit consists of the laser diode, imaging system, line sensor, scanning mirror, and one-board microcomputer. The speckle signal is analyzed and sent to a personal computer, where the blood flow in a tissue area of 19 cm × 25 cm is evaluated and displayed on a 2-D color map. It was found that the blood flow in the skin tissue sharply decreased under the sacrum when it was supported by a hard surface, while the decrease became negligible when the surface was replaced by a soft airbag.     

Optical Imaging and Tissue Characterization with Polarization Discrimination of Time-Gated Signals

In this paper we demonstrated the effectiveness of imaging in a tissue phantom with isotropic scattering using polarization discrimination combined with the time gating method. This simple polarization discrimination technique was shown ineffective when it was applied to filamentous tissues. In this situation, we utilized the time-gated degree of polarization (DOP) imaging technique to show that the DOP measurement was quite effective for high-quality imaging of objects in filamentous tissues. We also applied this technique to the characterization of myocardial tissues and showed the difference between normal and abnormal tissues. In addition, we demonstrated a novel method for target depth determination in a turbid medium based on co-polarized light measurements. This method relied on the strong dependence of transmitted co-polarized intensity on target depth.     

基于漫反射光谱法下层组织信息的最佳探测位置的研究

由于人体组织结构的复杂性和个体差异性,采用光谱分析技术实现组织内部信息的检测方法中,仍存在着检测位置不明确的问题。采用多位置空间分辨漫反射光谱法对脂肪肌肉组织中内部信息的最佳探测位置进行了研究。由于目标组织为肌肉组织,为了消除由于不同人体皮下脂肪层厚度的差异以及目标层中多次后向散射光对测量结果引起的较大误差,并根据光在复杂生物组织中的传输模型,通过增加约束条件——两条理想的“香蕉型”路径—来定义有效光子比SNR,并以SNR作为评价最佳探测位置SDS_best的标准,对优化后的Monte Carlo仿真结果进行统计分析,分别研究了脂肪层厚度h_f、脂肪层吸收系数μ_af和肌肉层吸收系数μ_am与光源探测器间距SDS之间的关系,并以h_f为自变量与SDS_best建立线性回归模型。研究表明,(1)当0<h_f<0.6 cm时,μ_af和μ_am对SDS_best几乎没有影响,而h_f与SDS_best的建模相关系数R2为0.991 8;(2)任取脂肪层厚度值0.12与0.22 cm作预测,得到预测误差分别为0.030 14和0.020 16,预测误差均能控制在5%以内。该方法能更方便、快速地为测量组织内部信息确定最佳检测位置,并有效削弱浅表组织以及目标层中多次背向散射光的干扰。