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1.
The individual antioxidants of spices (gallic and rosmarinic acids, capsaicin, thymol, and eugenol) are irreversibly oxidized at 0.88–1.25 V at a glassy carbon electrode in 0.1 M LiClO4 solution in ethanol. Corresponding electrode reactions are proposed. A linear dependence of the oxidation step area on the concentration is observed for all the analytes. The detection limits and the lower limits of quantification vary from 0.57–12 and 1.8–40 μM, respectively. Distinct steps and peaks of oxidation are observed on cyclic voltammograms of the methanolic extracts of spices; the potentials and areas of these peaks depend on the type of spice. The contribution of individual antioxidants to integral responses of spice extracts is evaluated. A method is developed for the voltammetric evaluation of the antioxidant capacity (AOC) of spices based on the oxidation of their antioxidants. The total area of the oxidation steps was selected as a parameter characterizing the antioxidant properties. The AOC of spices was expressed as a weight of gallic acid in milligrams per 1 g of a dry spice. Seventeen different spices were tested. The AOC decreases in the series of clove > juniper berries > nutmeg > cinnamon > rosemary > anise ≥ oregano > black pepper ≥ ginger ≥ basil > turmeric > red pepper ≈ bay leaf ≥ coriander ≈ red sweet pepper > cumin > caraway. A correlation between the AOC obtained by voltammetry and the total AOC, ferric reducing power, antiradical activity, and total content of phenolic compounds and these parameters with each other is found; the correlation coefficients vary in the range 0.8886–0.9615. 相似文献
2.
Antioxidants play a major role in protecting biological systems against many incurable diseases. The biological activity of 12 plant aqueous-alcohol extracts, some standard antioxidants (vitamin C, glucose, resorcinol, and catechol), Na(2)SO(3), humic acids, phthalocyanines, and chlorophyll have been investigated in this work together with evaluation of their influence on the kinetics of the oxygen electroreduction. Finally the use of these substances for prophylactic purposes has been recommended. 相似文献
3.
Study of antioxidant properties of a water-soluble Vitamin E derivative-tocopherol monoglucoside (TMG) by differential pulse voltammetry 总被引:1,自引:0,他引:1
Study of antioxidant properties of tocopherol monoglucoside (TMG), a water-soluble Vitamin E derivative, by differential pulse voltammetry has been carried out in this work. The pH influence on the antioxidant properties of TMG has been also investigated. It was observed that the antioxidant activity of TMG is greater at 6.90<pH<9.18. The reactions between the TMG and reactive oxygen species have been considered. Antioxidant activity of some standard antioxidants including Vitamin E was given for comparison. The results indicate that the TMG is an effective antioxidant in neutral solutions. The oxidation potential for TMG at pH 7.31 has been found (E=0.55±0.03 V versus Ag|AgCl|KClsat electrode). Finally the use of TMG for protection against oxidative stress has been recommended. 相似文献
4.
The electrochemical behavior of nine pyrrolidinofullerenes has been investigated by cyclic voltammetry on a gold microdisk electrode. Four reversible reduction peaks and two irreversible reduction peaks are observed for each fullerene derivative. The half-wave potentials of all pyrrolidinofullerenes are more negative than those of C60 itself. The diffusion coefficient of these compounds is measured by their steady-state voltammograms. 相似文献
5.
Van Hong NT Rivière C Hong QT Chataigné G Hoai NN Dejaegher B Tistaert C Kim TN Van Kiem P Vander Heyden Y Van MC Quetin-Leclercq J 《Natural product communications》2011,6(6):813-818
Several Mallotus species (Euphorbiaceae) are used in Vietnam as edible plants or as traditional medicines for different indications, some related to the treatment of inflammatory diseases. This study investigated the antioxidant activities of 33 samples from 17 Vietnamese Mallotus species. We also evaluated potential cytotoxic activity against human cervix carcinoma HeLa and human lung fibroblast WI-38 cells. Our aim is to develop safe dietary supplements with a protective effect against various diseases caused by tissue damage and the acceleration of the aging process linked to reactive oxygen species. These tests allowed the identification of non-cytotoxic plant species exhibiting significant antiradical properties. These antioxidant properties may be explained by their polyphenol composition. The antioxidant activity of the most active Mallotus species was further analyzed with and without tannins removal. We also identified by LC-ESI-MS some flavonoids responsible for a part of this activity. 相似文献
6.
Nafisi Sh Shadaloi A Feizbakhsh A Tajmir-Riahi HA 《Journal of photochemistry and photobiology. B, Biology》2009,94(1):1-7
Flavonoids are an interesting group of natural polyphenolic compounds that exhibit extensive bioactivities such as scavenging free radical, antitumor and antiproliferative effects. The anticancer and antiviral effects of these natural products are attributed to their potential biomedical applications. While flavonoids complexation with DNA is known, their bindings to RNA are not fully investigated. This study was designed to examine the interactions of three flavonoids; morin (Mor), apigenin (Api) and naringin (Nar) with yeast RNA in aqueous solution at physiological conditions, using constant RNA concentration (6.25 mM) and various pigment/RNA (phosphate) ratios of 1/120 to 1/1. FTIR, UV-visible spectroscopic methods were used to determine the ligand binding modes, the binding constant and the stability of RNA in flavonoid-RNA complexes in aqueous solution. Spectroscopic evidence showed major binding of flavonoids to RNA with overall binding constants of K(morin) = 9.150 x 10(3) M(-1), K(apigenin)=4.967 x 10(4) M(-1), and K(naringin)=1.144 x 10(4) M(-1). The affinity of flavonoid-RNA binding is in the order of apigenin>naringin>morin. No biopolymer secondary structural changes were observed upon flavonoid interaction and RNA remains in the A-family structure in these pigment complexes. 相似文献
7.
Okumura LL Regasini LO Fernandes DC da Silva DH Zanoni MV Bolzani Vda S 《Journal of AOAC International》2012,95(3):773-777
A fast, low-cost, convenient, and especially sensitive voltammetric screening approach for the study of the antioxidant properties of isoquercitrin and pedalitin from Pterogyne nitens is suggested in this work. These flavonoids were investigated for their redox properties using cyclic voltammetry in nonaqueous media using N,N-dimethylformamide and tetrabutylammonium tetrafluorborate as the supporting electrolyte, a glassy carbon working electrode, A6(see symbol in text)AgCI reference electrode, and Pt bare wire counter electrode. The comparative analysis of the activity of rutin has also been carried out. Moreover, combining HPLC with an electrochemical detector allowed qualitative and quantitative detection of micromolecules (e.g., isoquercitrin and pedalitin) that showed antioxidant activities. These results were then correlated to the inhibition of beta-carotene bleaching determined by TLC autographic assay and to structural features of the flavonoids. 相似文献
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9.
The non-Faradaic preconcentration behaviour of nine flavonoids (six flavones: fisetin, galangin, morin, quercetin, rhamnetin, rutin, and three flavanones: hesperidin, hesperitin, naringin) at a carbon-paste (nujol/graphite) electrode and the factors affecting it (pH, accumulation potential, presence of various surfactants) for their subsequent differential pulse voltammetric determination are examined. All flavones tested are readily accumulated on the carbon paste electrode resulting in a considerable signal enhancement making determinations feasible down to 10−8 − 10−7 M after preconcentration for 1–4 min. Flavanones are not preconcentrated so their lower determination limits are of the order of 10-6 M. A simple voltammetric procedure for the determination of rutin in a multivitamin preparation is presented. 相似文献
10.
Summary Antioxidant flavonoids from the plantsSolidago gigantea Ait.,Taraxacum officinale Wiggers and Webers (Asteraceae) andMorus nigra L. (Moraceae) have been analysed by capillary electrophoresis (CE).Solidago gigantea was investigated because of its diuretic, spasmolytic, antiphlogistic, and wound-healing effect,Taraxacum officinale because it has been shown to have good diuretic and choleretic activity, andMorus nigra because it is also widely regarded as a diuretic and antidiabetic agent. Aqueous and methanolic extracts of these plants
have antioxidant properties. Because their flavonoid composition might be important in their free-radical-scavenging activity,
a capillary electrophoretic method was developed for characterization of the flavonoids present. We identified quercetin-3-O-β-rutinoside (rutin), quercetin-3-O-β-d-glucoside (isoquercitrin), and chlorogenic acid as the most abundant compounds inSolidago gigantea andMorus nigra, and apigenin-7-O-β-glucoside, luteolin-7-O-β-glucoside, and chlorogenic acid inTaraxacum officinale. We also discovered that quercetin-3-O-α-rhamnoside (quercitrin) and quercetin-3-O-β-galactoside (hyperoside) were absent from our sample ofSolidago gigantea and quercitrin fromMorus nigra. Quantitative analysis of these extracts was performed by high-performance liquid chromatography (HPLC).
Presented at Balaton Symposium '01 on High-Performance Separation Methods, Siófok, Hungary, September 2–4, 2001 相似文献
11.
In this study the antioxidant and cytotoxicity activity of the Adonidia merrillii fruits were investigated using different solvent polarities (methanol, ethyl acetate and water). The results showed that the total phenolic and flavonoid contents of the methanolic extract was higher compare with other extract with respective values of 17.80 ± 0.45 mg gallic acid equivalents/g dry weight (DW) and 5.43 ± 0.33 mg rutin equivalents/g DW. Beside that The RP-HPLC analyses indicated the presence of gallic acid, pyrogallol, caffeic acid, vanillic acid, syringic acid, naringin and rutin. In the DPPH, NO2 and ABTS scavenging assays, the methanolic extract exhibited higher antioxidant activity as compared to the ethyl acetate and water extracts. The extracts exhibited moderate to weak cytotoxic activity in the assays using human hepatocytes (Chang liver cells) and NIH/3T3 (fibroblasts cell) cell lines. The findings showed the Adonidia merrillii fruit extracts to possess considerable antioxidant and cytotoxicity properties. The fruit, therefore, is a potential candidate for further work to discover antioxidant and cytotoxic drugs from natural sources. 相似文献
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13.
The inclusion complexes of a series of organometallic compound-cyclodextrin and aromatic compound-cyclodextrin have been studied by cyclic voltammetry using glassy carbon electrode. The variations of peak potential and peak current are showed on cyclic voltammogram when the electroactive guest molecules are complexed by cyclodextrins. Dissociation constants of cyclodextrin inclusion complexes have been calculated on the basis of this variation by both potential and current methods. According to the magnitude of dissociation constants the relationship between the stability of cyclodextrin inclusion complex and the degree of matching host molecule with guest molecule has been discussed. 相似文献
14.
The possibility of determination of twelve flavonoids (fisetin, galangin, hesperetin, hesperidin, kaempherol, morin, myricetin, naringin, quercetin, quercitrin, rutin, rhamnetin) using adsorptive stripping voltammetry in a flow injection system is examined. Carbon paste electrodes based on mixtures of nujol-graphite and diphenylether-graphite were used and compared as working electrodes and measurements were made using both 'direct' and 'medium-exchange' procedures. The effect of experimental parameters such as pH, accumulation potential and period, presence of various compounds was examined. Sample size (typically 0.33 ml) is greatly reduced by applying a reciprocating flow of the sample plug through the detector during the preconcentration step. The instrumental sensitivities and lower detection limits were calculated for all flavonoids in all cases. In some cases the differences are so high that determination of certain flavonoids in the presence of excess of others is allowed. Depending on the flavonoid, the electrode type, and the measurement procedure the analytical range and the reproducibility (RSD%) were 10(-5)-10(-8) M and 2.5-10%, respectively. A simple procedure is developed for the electrochemical determination of rutin in a multivitamin preparation. 相似文献
15.
E. P. Kemertelidze V. G. Tsitsishvili M. D. Alaniya T. G. Sagareishvili 《Chemistry of Natural Compounds》2000,36(1):54-59
The recombination kinetics of a series of flavonoids with stable DPPH radicals are studied. Flavonols are the most reactive. Polarization of the carbonyl in flavones reduces the reactivity. Substitution on C-7 and the B ring has practically no effect on the reactivity. Glycosylation of C-3 reduces the reactivity if the sugar can form two intramolecular H-bonds. The reactivity of the flavonoid phenol hydroxyls, with the exception of the hydroxyl on C-5, is proportional to their number. The most promising antioxidants are lespedin, kaempferitrin, kaempferol, kaempferol-7-rhamnoside, kaempferol-3-robinobioside, and robinin.I. G. Kutateladze Institute of Pharmacochemistry, Georgian Academy of Sciences, Tbilisi, fax (99532)-25-00-26. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 42–46, January–February, 2000. 相似文献
16.
Cyclopentadienylcyclooctatetraenetitanium and some other substituted cyclopentadienyl compounds of the same structure have been studied by cyclic voltammetry. They show a perfectly reversible one-electron oxidation wave at relatively low potentials. They are not reducible to 18-electron species. 相似文献
17.
环糊精包络物的循环伏安法研究 总被引:23,自引:0,他引:23
本文采用玻碳电极以循环伏安法研究了水溶液体系中二茂铁衍生物及芳香族衍生物与环糊精(α-CD, α-CD)的包络行为. 当电活性客体分子被包络时, 在循环伏安图上表现为峰电流和峰电位的变化, 用电流和电位法测定了包络物的解离常数, 并根据解离常数的大小次序探讨了主体分子与客体分子之间的匹配情况同包络物稳定性之间的关系. 相似文献
18.
Zheleva-Dimitrova D Nedialkov P Girreser U Kitanov G 《Natural product research》2012,26(17):1576-1583
The occurrence of three known benzophenones, namely annulatophenonoside, acetylannulatophenonoside and annulatophenone as well as a flavonol O-glycoside guajaverin in the aerial parts of Hypericum maculatum Crantz was established. In addition, hyperoside, isoquercitrin and miquelianin were isolated from this plant, as well. Radical scavenging and antioxidant activities of the isolated compounds were examined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) free radicals, ferric reducing antioxidant power (FRAP) assay and inhibition of lipid peroxidation in linoleic acid system by the ferric thiocyanate method. Isoquercitrin demonstrates the highest DPPH radical scavenging (96.6?±?0.3%), FRAP (23.8?±?0.2 Trolox equivalent, TE?mol?1) and antioxidant activity in linoleic acid system. Guajaverin and acetylannulatophenonoside show significantly strong ABTS radical scavenging activity (93.9?±?0.4% and 93.4?±?0.6%, respectively), which is comparable to that of ascorbic acid (96.2?±?0.4%). 相似文献
19.
The present study was undertaken to investigate the individual and combined antioxidant or prooxidant effects of genistein, daidzein and quercetin in human erythrocytes and rat microsomes in vitro. Their reducing potential against oxidation of a redox sensitive fluorescent probe, their protective effect against H2O2-induced membrane lipid peroxidation and their inhibitory effect on AAPH-induced hemolysis were evaluated. Genistein and daidzein were prooxidant in erythrocytes but antioxidant in microsomes where their metabolites might have been formed which suggests the importance of metabolic capacity in in vitro models to predict the physiological situation. Quercetin showed antioxidant effects in all models and conditions. Prooxidant effect of ‘genistein–daidzein mixture’, at their concentrations reflecting the real life, was suppressed by addition of quercetin to the mixture. Our study shows that flavonoids can exert prooxidant effects depending on the conditions, but the mixture effect should be considered while assessing their effects and safety in humans. 相似文献
20.
Estimation of the antioxidant activities of flavonoids from their oxidation potentials. 总被引:7,自引:0,他引:7
A simple electrochemical method for estimating the antioxidant activity (AA) of flavonoids has been developed. The proposed method is based on a measurement of the half-wave potential (E1/2) of the first oxidation wave of flavonoids by using flow-through column electrolysis. At the same time, the lipid peroxidation (LPO) inhibiting effects of these flavonoids were determined. A quantitative structure-activity relationship was obtained to describe the AA of flavonoids: IC50(microM) = 30.36 + 151.50 E1/2 (V) -12.63 log P (r = 0.852), where IC50 represents the concentration for 50% inhibition of LPO, and P represents the octanol/water partition coefficient. This method is expected to be useful for the quick screening of flavonoid antioxidants, and evaluating the AA of flavonoid-containing foods and medicinal plants. 相似文献