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综述了固定化联吡啶钌[Ru(bpy)23+]电化学发光免疫传感技术的发展状况,介绍了近年来在电化学发光免疫传感领域出现的新型固相载体材料和固定方法,及其与其他分析技术联用方面的发展,并对其发展趋势进行了展望.指出电化学发光免疫分析技术在生物分子检测、药物分析及临床诊断中显示出强大的生命力.Ru(bpy)23+的电化学发光已引起广泛的关注.固定化Ru(bpy)23+电化学发光免疫分析体系具有线性范围宽、灵敏度高、装置简单、可控性强等优点,被广泛应用于分子生物学、化学、药学等领域. 相似文献
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基于分子印迹技术的电化学发光分析是近几年刚刚发展起来的新型分析方法,兼具分子印迹技术与电化学发光方法两者的优点,具有高灵敏度、高选择性、可控性好、易于微型化和操作简单等特点,在生命科学、食品安全及环境监测等领域有着广泛的应用前景。本综述简要介绍了常用的电化学发光体系和基本原理,综述了近年来分子印迹电化学发光分析的主要研究进展,对不同类型分子印迹电化学分析的构建方法、原理及所构建方法的性能(包括灵敏度、选择性、检测范围和稳定性等)进行了评述。基于分子印迹技术的电化学发光分析主要可以分为三类:制备固态发光电极、非固态发光电极构建分子印迹电化学发光传感器和分子印迹固相萃取与电化学发光分析联用,其中制备固态发光电极用于构建分子印迹电化学发光传感器最有发展前景。最后,本综述也对分子印迹电化学发光分析今后的发展趋势和方向进行了展望。 相似文献
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电化学发光免疫传感技术在生物药物分析中的研究进展 总被引:1,自引:0,他引:1
随着生物及药物分析领域的不断扩展,发展高灵敏度及高选择性的分析手段以解决复杂样品体系中低浓度待测物的分析测试问题是十分迫切的需求.电化学发光分析方法由于具有线性范围宽,灵敏度高及可控性强等优点,是处理低浓度样品的有效工具.这种方法与免疫传感技术相结合,有利于实现生物体液等复杂样品中极低含量生化物质与药物的高选择性、高灵敏度检测.本文综述了电化学发光免疫传感技术的发展状况,介绍了近年来在电化学发光免疫传感中出现的新型固相载体、电化学发光探针和共反应物、以及多组分免疫传感技术等,并对其在生物药物分析中的应用情况进行了总结. 相似文献
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指纹在法医鉴定及人身识别方面担任着重要作用,同时广泛应用于日常生活中的安全检验、访问控制、个人认证等领域。当前尽管指纹显现方法繁多,但是仍然需要一种技术简单、快速、容易操作的方法对指纹进行显现。本文基于选择性空间控制鲁米诺在电极表面的电化学发光行为,构建了一种潜在指纹的反相成像技术。考察了共反应剂种类、施加电位以及发光体浓度对指纹显现效果的影响。该成像方法具有简单、快速以及无需对基底或样本进行处理等优点,为指纹显现和电化学成像技术提供了一种新方法和新思路。 相似文献
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Fangxin Du Yequan Chen Chengda Meng Baohua Lou Wei Zhang Guobao Xu 《Current Opinion in Electrochemistry》2021
Electrochemiluminescence (ECL) has been widely applied in immunoassays because of low background, high sensitivity, and excellent spatiotemporal controllability. In order to meet the increasing demand for high efficiency and accuracy of immunoassays in complex conditions, considerable efforts have been devoted to ECL strategies with multiple-signal outputs. In this short review, we summarize the recent advances of ECL strategies based on multiple-signal outputs, which includes ratiometric ECL, ECL multiplex immunoassay (MIA), and ECL imaging. Their signal generation strategies and analysis applications for immunoassay are discussed in detail. Moreover, the challenges and prospects in this field from us are addressed. 相似文献
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In this review, the basic principles and apparatus of ECL imaging were briefly introduced at first. Then several latest and representative applications of ECL imaging based on nanomaterials and micro-/nanostructures were overviewed. Finally, the superiorities and challenges in ECL imaging for further development were discussed. 相似文献
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Electrochemiluminescence (ECL) is a kind of luminescent phenomenon caused by electrochemical reactions. Based on the advantages of ECL including low background, high sensitivity, strong spatiotemporal controllability and simple operation, ECL imaging is able to visualize the ECL process, which can additionally achieve high throughput, fast and visual analysis. With the development of optical imaging technique, ECL imaging at micro- or nanoscale has been successfully applied in immunoassay, cell imaging, biochemical analysis, single-nanoparticle detection and study of mechanisms and kinetics of reactions, which has attracted extensive attention. In this review, the basic principles and apparatus of ECL imaging were briefly introduced at first. Then several latest and representative applications of ECL imaging based on nanomaterials and micro-/nanostructures were overviewed. Finally, the superiorities and challenges in ECL imaging for further development were discussed. 相似文献
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Electrogenerated chemiluminescence, also known as electrochemiluminescence (ECL), is an electrochemically induced production of light by excited luminophores generated during redox reactions. It can be used to sense the charge transfer and related processes at electrodes via a simple visual readout; hence, ECL is an outstanding tool in analytical sensing. The traditional ECL approach measures averaged electrochemical quantities of a large ensemble of individual entities, including molecules, microstructures and ions. However, as a real system is usually heterogeneous, the study of single entities holds great potential in elucidating new truths of nature which are averaged out in ensemble assays or hidden in complex systems. We would like to review the development of ECL intensity and imaging based single entity detection and place emphasis on the assays of small entities including single molecules, micro/nanoparticles and cells. The current challenges for and perspectives on ECL detection of single entities are also discussed.We summarize the history and recent development that has been made in the ECL detection of single entities. 相似文献
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由于方法的使用范围广、光学系统简单和操作容易,电致化学发光(ECL)得到人们的广泛重视。随着对ECL研究的深入,ECL研究所涉及的领域和层面已有很大的扩展,特别是近十年来,ECL研究发展更为迅猛。除ECL理论研究外,为了适应分析检测的应用的需求,ECL在新材料、新实验技术和方法方面出现了许多的研究报道。本文综述最近几年来ECL研究在新材料应用和新实验技术的开发方面的一些进展,包括纳微米材料和量子点材料在ECL方面的研究,同时对固态ECL和基于三原色(RGB)机理的可视化ECL研究进展,进行了一些讨论。最后,综述展望纳米和量子点材料修饰电极ECL的研究和应用的前景。 相似文献
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Electrochemiluminescence (ECL) is the light production triggered by reactions at the electrode surface. Its intrinsic features based on a dual electrochemical/photophysical nature have made it an attractive and powerful method across diverse fields in applied and fundamental research. Herein, we review the combination of ECL with semiconductor (SC) materials presenting various typical dimensions and structures, which has opened new uses of ECL and offered exciting opportunities for (bio)sensing and imaging. In particular, we highlight this particularly rich domain at the interface between photoelectrochemistry, SC material chemistry and analytical chemistry. After an introduction to the ECL and SC fundamentals, we gather the recent advances with representative examples of new strategies to generate ECL in original configurations. Indeed, bulk SC can be used as electrode materials with unusual ECL properties or light-addressable systems. At the nanoscale, the SC nanocrystals or quantum dots (QDs) constitute excellent bright ECL nano-emitters with tuneable emission wavelengths and remarkable stability. Finally, the challenges and future prospects are discussed for the design of new detection strategies in (bio)analytical chemistry, light-addressable systems, imaging or infrared devices.The combination of electrochemiluminescence and semiconductor gives rise to a rich field at the interface of photoelectrochemistry, materials and analytical chemistry. It offers interesting possibilities for ultrasensitive (bio)detection, imaging and light conversion. 相似文献
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《中国化学快报》2021,32(8):2369-2379
Living-cell imaging demands high specificity,sensitivity,and minimal background interference to the targets of interest.However,developing a desirable imaging probe that can possess all the above features is still challenging.The bioorthogonal surface-enhanced Raman scattering(SERS) imaging has been recently emerged through utilizing Raman reporters with characteristic peaks in Raman-silent region of cells(1800-2800 cm~(-1)),which opens a revolutionary avenue for living-cell imaging with multiplexing capability.In this review,we focus on the recent advances in the technology development and the biological and biomedical applications of the living-cell bioorthogonal SERS imaging technique.After introduction of fundamental principles for bioorthogonal tag or label,we present applications for visualization of various intracellular components and environment including proteins,nucleic acids,lipids,pH and hypoxia,even for cancer diagnosis in tissue samples.Then,various bioorthogonal SERS imaging-guided thera py strategies have been discussed such as photothera py and surge ry.In conclusion,this strategy has great potential to be a flexible and robust tool for visualization detection and diseases diagnosis. 相似文献
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Image‐Contrast Technology Based on the Electrochemiluminescence of Porous Silicon and Its Application in Fingerprint Visualization
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Jie Tan Linru Xu Tong Li Prof. Bin Su Prof. Jianmin Wu 《Angewandte Chemie (International ed. in English)》2014,53(37):9822-9826
The electrochemiluminescence (ECL) of porous silicon (pSi) has attracted great interest for its potential application in display technology and chemical sensors. In this study, we found that pSi with a different surface chemistry displayed an apparently different dynamic ECL process. An image‐contrast technology was established on the basis of the intrinsic mechanism of the ECL dynamic process. As a proof of principle, the visualization of latent fingerprints (LFPs) and in situ detection of TNT in fingerprints was demonstrated by using the ECL‐based image‐contrast technology. 相似文献
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In recent years,there has been increasing interest in the development of electrochemi-luminence (ECL) in HPLC for the analysis of various biological substances because of its high sensitivity. ECL is a detection technique based on electrolytically generated chemilumi-narscence. 相似文献