Microwave-assisted extraction followed by gas chromatography-mass spectrometry for the determination of endocrine disrupting chemicals in river sediments

A method for the simultaneous measurement of trace amounts of phenolic xenoestrogens, such as 2,4-dichlorophenol (2,4-DCP), 4-tert-butyl-phenol (BP), 4-tert-octylphenol (OP), 4-nonylphenol (NP), pentachlorophenol (PCP) and bisphenol A (BPA), in water samples was developed using stir bar sorptive extraction (SBSE) with in situ derivatization followed by thermal desorption (TD)-gas chromatography-mass spectrometry (GC-MS) analysis. The conditions for derivatization with acetic acid anhydride were investigated. A polydimethylsiloxane (PDMS)-coated stir bar and derivatization reagents were added to 10 ml of water sample and stirring was commenced for 10-180 min at room temperature (25 degrees C) in a headspace vial. Then, the extract was analyzed by TD-GC-MS. The optimum time for SBSE with in situ derivatization was 90 min. The detection limits of 2,4-DCP, BP, OP, NP, PCP and BPA were 2, 1, 0.5, 5, 2 and 2 pg ml(-1), respectively. The method showed good linearity over the concentration ranges of 10, 5, 2, 20, 10 and 10-1000 pg ml(-1) for 2,4-DCP, BP, OP, NP, PCP and BPA, respectively, and the correlation coefficients were higher than 0.99. The average recoveries of those compounds in river water samples were equal to or higher than 93.9% (R.S.D. <7.2%) with correction using the added surrogate standards. This simple, accurate, sensitive and selective method can be used in the determination of trace amounts of phenolic xenoestrogens in river water samples.     

Direct analysis of trace level bisphenol A,octylphenols and nonylphenol in bottled water and leached from bottles by ultra‐high‐performance liquid chromatography/tandem mass spectrometry

A high‐throughput method was developed for the direct analysis of trace level bisphenol A (BPA), 4‐n‐octylphenol (4‐n‐OP), 4‐tert‐octylphenol (4‐t‐OP) and 4‐n‐nonylphenol (4‐n‐NP) in water samples by ultra‐high‐performance liquid chromatography/tandem mass spectrometry (uHPLC/MS/MS) using an isotope‐labeled internal standard. Aliquots of water samples were spiked with the internal standard and analyzed without sample cleanup or enrichment. All target analytes were chromatographically separated within 3 min and detected using the highly selective multiple reaction monitoring (MRM) detection mode. The method detection limits were statistically calculated and ranged from 0.040 ppb (BPA) to 0.057 ppb (4‐n‐NP). Excellent correlation of determination was achieved for each analyte with r greater than 0.995. Precision was achieved within 8% relative standard deviation (RSD) for all analytes, and recoveries from spiked samples ranged from 97% to 106.2% except for 4‐n‐OP which may be corrected by using an isotope‐labeled analog as internal standard. This method was used for analyzing eight randomly selected bottled water samples and found no detectable target analytes. This method was also used to analyze target analytes leached from bottles (6 low cost bottles and 6 brand‐name baby‐feeding bottles). Low levels of BPA were found in three bottles after they had been heated in a microwave oven, and a trace amount of 4‐n‐NP was also found in three bottles. 4‐t‐OP, which has not been reported as a leachable chemical, was found in two brand name baby‐feeding bottles from the same manufacturer, and was confirmed with bottles from different batches. Copyright © 2010 John Wiley & Sons, Ltd.     

加速溶剂萃取-液相色谱-质谱/质谱法分析动物组织中的壬基酚、辛基酚和双酚A

建立了测定内分泌干扰物质烷基酚、双酚A的液相色谱-电喷雾串联质谱(负离子模式)分析方法,优化了样品前处理方法。以二氯甲烷作提取溶剂,采用加速溶剂萃取法萃取动物组织样品,萃取液用500 mg OASIS氨基固相萃取柱进行浓缩净化。对流动相组分和流动相添加剂对质谱的离子化效率进行了考察,测得3种化合物在高、中、低3个添加水平的回收率为88%~101%,相对标准偏差小于15%;双酚A、壬基酚和辛基酚的方法检出限分别为0.3, 0.05和0.1 μg/kg。对从北京市场上采集的27份动物组织样品进行检测,结果表明壬基酚广泛存在于各种动物源性食品中,检出含量为0.49~55.98 μg/kg,其中鱼肉组织中都检出壬基酚,而且其含量也较高(9.13~55.98 μg/kg)。     

固相萃取高效液相色谱质谱法测定海水中烷基酚和烷基酚聚氧乙烯醚

建立了固相萃取-高效液相色谱-电喷雾质谱分析海水中辛基酚、壬基酚、辛基酚聚氧乙烯醚和壬基酚聚氧乙烯醚的方法。海水样品经C18固相萃取柱富集净化后,以甲醇-水为流动相,在Hypersil GOLD色谱柱上分离,电喷雾质谱在选择离子监测模式下分析目标化合物,采用外标法定量。结果表明,4种化合物的平均加标回收率为59.6%～104.4%,相对标准偏差(RSD, n=3)为1.0%～13.5%;仪器检出限为0.08～3 μg/L。将本方法用于大连海岸6个采样点海水中辛基酚、壬基酚、辛基酚聚氧乙烯醚和壬基酚聚氧乙烯醚的检测发现,样品中壬基酚和壬基酚聚氧乙烯醚均有检出,且油港和海港附近海水中的含量较高。     

Chemical monitoring and occurrence of alkylphenols, alkylphenol ethoxylates, alcohol ethoxylates, phthalates and benzothiazoles in sewage treatment plants and receiving waters along the Ter River basin (Catalonia, N. E. Spain)

This study presents a quantitative estimation of the analysis and fate of several emerging pollutants, some of them endocrine-disrupting compounds, in surface water samples collected at several locations along the Ter River and two of its tributaries. Influent and effluent waters and particulate matter from five sewage treatment plants (STP) that discharge into these rivers were also studied. The target compounds analyzed were: nonylphenol ethoxylates (NPEO), nonylphenol (NP), octylphenol (OP), bisphenol A (BPA), phthalates, alcohol ethoxylates (AEO) and benzothiazoles. Chemical analysis by liquid chromatography–mass spectrometry using an electrospray interface (LC–ESI–MS) revealed the presence of low amounts (between 0.06 and 17.5 μg L⁻¹) of the target compounds NPE₁₊₂O and NP, which were detected in 100% and 84% of the samples respectively. Maximum concentrations occurred in the STPs associated with the municipalities of Vic and Girona. From the fate and behavior data obtained for the various compounds analyzed in the STP influent and effluent, we can conclude that the STPs are effective at removing large amounts (more than 70%) of the compounds studied from the water.      

Simultaneous determination of the endocrine disrupting compounds nonylphenol, nonylphenol ethoxylates, triclosan and bisphenol A in wastewater and sewage sludge by gas chromatography-mass spectrometry

An integrated analytical method for the simultaneous determination of 4-n-nonylphenol (4-n-NP), nonylphenol monoethoxylate (NP1EO), nonylphenol diethoxylate (NP2EO), bisphenol A (BPA) and triclosan (TCS) in wastewater (dissolved and particulate phase) and sewage sludge was developed based on gas chromatography-mass spectrometry. Chromatographic analysis was achieved after derivatization with bis(trimethylsilyl)trifluoroacetamide (BSTFA). Extraction from water samples was performed by solid-phase extraction (SPE). The optimization of SPE procedure included the type of sorbent and the type of the organic solvent used for the elution. Referred to solid samples, the target compounds were extracted by sonication. In this case the optimization of the extraction procedure included the variation of the amount of the extracted biomass, the duration and the temperature of sonication and the type of the extraction organic solvent. The developed extraction procedures resulted in good repeatability and reproducibility with relative standard deviations (RSDs) less than 13% for all the tested compounds for both types of samples. Satisfactory recoveries were obtained (>60%) for all the compounds in both liquid and solid samples, except for 4-n-NP, which gave recoveries up to 35% in wastewater samples and up to 63% in sludge samples. The limits of detection (LODs) of the target compounds varied from 0.03 (4-n-NP) to 0.41 microg l(-1) (NP2EO) and from 0.04 (4-n-NP) to 0.96 microg kg(-1) (NP2EO) for liquid and solid samples, respectively. The developed methods were successfully applied to the analysis of the target compounds in real samples.     

Determination of nonylphenol and octylphenol in paper by microwave-assisted extraction coupled to headspace solid-phase microextraction and gas chromatography-mass spectrometry

A novel and simple method for the determination of active endocrine disrupter compounds (octylphenol OP, and nonylphenol NP) in paper using microwave-assisted extraction (MAE) and headspace solid-phase microextraction, coupled with gas chromatography-mass spectrometry has been developed. Parameters affecting the efficiency in the MAE process such as exposure time and extraction solvent were studied in order to determine operating conditions. The optimised method was linear over the range studied (1.25-125 microg kg(-1) for OP and 9.50-950 microg kg(-1) for NP) and showed good level of precision, with a RSD lower than 10% and detection limits at 0.10 and 4.56 microg kg(-1) for OP and NP, respectively. The results obtained from six different types of paper revealed the presence of the target compounds in all samples analysed, at levels ranging between 3 and 211 microg kg(-1).     

Occurrence of endocrine-disrupting compounds in reclaimed water from Tianjin,China

Continuous disposal of endocrine-disrupting compounds (EDCs) into the environment can lead to serious human health problems and can affect plants and aquatic organisms. The determination of EDCs in water has become an increasingly important activity due to our increased knowledge about their toxicities, even at low concentration. The EDCs in water samples from the reclaimed water plant of Tianjin, northern China, were identified by gas chromatography (GC)–mass spectrometry (MS). Important and contrasting EDCs including estrone (E1), 17β-estradiol (E2), 17α-ethynylestradiol (EE2), 4-tert-octylphenol (OP), 4-nonylphenol (NP), bisphenol A (BPA), di-n-butyl phthalate (DnBP), diisobutyl phthalate (DIBP), and di(2-ethylhexyl)phthalate (DEHP) were selected as the target compounds. Concentrations of steroid hormones, alkylphenolic compounds and phthalates ranged from below the limit of detection (LOD) to 8.1 ng L⁻¹, from <LOD to 14.2 ng L⁻¹, and from 1.00 μg L⁻¹ to 23.8 μg L⁻¹, respectively. The average removal efficiencies for target EDCs varied from 30% to 82%. These results indicate that environmental endocrine disrupting compounds are not completely removed during reclaimed water treatment and may be carried over into the general aquatic environment.     

Determination of endocrine disrupting chemicals in environmental solid matrices by extraction with a non-ionic surfactant (Tween 80)

A readily applicable method based on extraction by aqueous non-ionic surfactant solutions (Tween 80) and RP-HPLC coupled to fluorescence detection, has been developed for the simultaneous determination of the phenolic endocrine disrupting chemicals (EDCs) nonylphenol (NP), nonylphenol monoethoxylate (NP1EO) and nonylphenol diethoxylate (NP2EO) and bisphenol A (BPA) in environmental solid matrices. Clean up of sample extracts was performed on Si-C18 solid phase extraction (SPE) cartridges. The overall Tween 80 extraction-SPE-RP-HPLC procedure was validated for accuracy and precision by analyzing sediment samples spiked with known amounts of EDCs. Recoveries for NP, NP1EO, NP2EO and BPA and limits of detection are in agreement with conventional extraction methods. The developed methodology was successfully applied to the analysis of target compounds in Italian river sediments, river suspended matter and benthonic macroinvertebrate organisms (oligochaetes Lumbriculus variegatus). Results confirmed that this relatively simple procedure performed satisfactorily in the determination of phenolic EDCs in environmental solid matrices of different complexity and that it can be a suitable alternative method to conventional systems even for routine analyses.     

Determination of phenolic xenoestrogens in environmental samples by liquid chromatography with mass spectrometric detection

A method is proposed for the determination of several phenolic xenoestrogens in aqueous and solid environmental samples. The method uses solid-phase extraction (preceded by ultrasonic solvent extraction for solid samples), reversed-phase liquid chromatographic separation, and mass spectrometric detection using both atmospheric pressure chemical ionization and electrospray ionization. This method was developed to support several studies undertaken to obtain aquatic and sedimentary data for rivers and seashores in Spain that are likely to be contaminated by endocrine-disrupting compounds (EDCs) as a consequence of wastewater discharge. Nonylphenol polyethoxylates (NPEOs), nonylphenoxy carboxylates (NPECs), nonylphenol (NP), octylphenol (OP), and bisphenol A (BPA) were determined in various samples of surface water and sediment, collected at different locations upstream and downstream from outfalls of municipal wastewater treatment plants (WWTPs). Seawater and marine sediments were collected in different harbor areas in Spain. Additionally, WWTP influent and effluents were analyzed to monitor the occurrence and transformation of phenolic EDCs during physicochemical and biological treatment. Rather high concentrations of the compounds investigated were found in some samples. Concentrations of NP were < or = 590 microg/kg in sediments and < or = 15 microg/L in water samples. NPEOs and NPECs were found in water samples in concentrations < or = 41 and < or = 35 microg/L, respectively. In solid samples (river sediment), concentrations of NPEO were < or = 818 microg/kg and those of NP1EC were 95 microg/kg.     

Optimizing gas chromatographic-mass spectrometric analysis of selected pharmaceuticals and endocrine-disrupting substances in water using factorial experimental design

An analytical method based on gas chromatography-mass spectrometry (GC-MS) has been developed to simultaneously determine selected acidic and neutral pharmaceuticals and endocrine-disrupting substances in surface and tap water. Solid-phase extraction (SPE) with Oasis HLB cartridges is followed by derivatization of the target analytes in the eluted extract. Derivatization was systematically optimized by employing a factorial experimental design. More specifically a central composite design was applied to search for the optimal conditions of the derivatization process and it was demonstrated that N-methyl-N-tert-butyl-dimethysilyl-trifluoroacetamide (MTBSTFA) had a better overall performance compared to N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA). The influence of solvent ratios and elution volumes while using SPE were also studied using a factorial design. The method was developed successfully for most of the selected compounds [i.e. ibuprofen, salicylic acid, gemfibrozil, naproxen, triclosan, propranolol, diclofenac, carbamazepine, 4-octylphenol (OP), 4-nonylphenol (NP), nonylphenol-monoethoxylate (NP1EO), nonylphenoxyacetic acid (NP1EC), estrone (E1), and 17alpha-ethinyloestradiol (EE2)]. Relative recoveries for spiked river and tap water ranged from 47 to 130% and 60-109%, respectively. Typical limits of detection were less than 5 ng/L in tap water and less than 10 ng/L in river water. Twelve target compounds were detected in river and tap water samples using the developed method. This method is currently used in bench-scale drinking water treatment studies.     

Determination of bisphenol A and 4-nonylphenol in human milk using alkaline digestion and cleanup by solid-phase extraction.

A highly sensitive and selective method based on alkaline digestion for the simultaneous determination of bisphenol A (BPA) and 4-nonylphenol (NP) was developed. The method consists of digestion of the matrix with ethanolic KOH, extraction with diethyl ether under a mild alkaline condition, cleaning with successive aminopropyl (NH2) cartridges and derivatization followed by a GC-MS analysis. The assay accuracies, expressed as recoveries, were 82 - 113% for BPA and 89 - 97% for NP. The limits of detection of BPA and NP were 0.09 ng/g and 0.50 ng/g, respectively. The procedure will be reliable for the trace analysis of BPA and NP in human milk, since alkaline digestion can diminish their documented association with protein.     

Determination of Estrogenic Nonylphenol and Bisphenol a in River Water by Solid‐Phase Extraction and Gas Chromatography‐Mass Spectrometry

A simple and sensitive method is presented for the analysis of nonylphenol (NP) and bisphenol A (BPA), two well known hormonally active agents (HAAs), in the samples of river water. The method involves extraction of the sample by a graphitized carbon black (GCB) solid‐phase extraction, and determination by an ion‐trap gas chromatography‐mass spectrometry (GC‐MS). The large‐volume injection technique provides high precision and sensitivity for NP and BPA, to quantitation at < 0.05 μg/L in 200 mL of water samples. Recovery of NP and BPA in spiked water samples ranged from 80% to 85%. Relative standard deviations (RSD) of replicate analyses ranged from 1.6% to 6.9%. The concentrations of NP in rivers were in the range between 0.4 to 2.4 μg/L, which were below the threshold concentration (10 μg/L) for vitellogenin induction in fish, but 78%) of water samples from five rivers exceeded the predicted‐no‐effect concentration (PNEC) of 0.7 μg/L as proposed recently. The concentrations of BPA ranged from < 0.05 μg/L to 3.0 μg/L, which all were below the PNEC of 64 μg/L.     

Determination of various estradiol mimicking-compounds in sewage sludge by the combination of microwave-assisted extraction and LC-MS/MS

In this work, we present the development and application of a microwave assisted extraction followed by liquid chromatography-tandem mass spectrometry methodology (MAE-LC-MS/MS) for the determination of various estradiol-mimicking compounds in sewage sludge samples. For the purification of the MAE extracts, we have employed a solid phase extraction (SPE) clean-up procedure, previously optimised. The entire method provides recoveries between 71.7% and 103.1%, with relative standard deviation lower than 11.1% and limits of detection ranging from 0.6 to 3.5 ng g⁻¹. The developed method was applied to samples from three wastewater treatment plants (WWTPs) located in Las Palmas of Gran Canaria (Spain), two of which had a conventional activated sludge treatment (AST), whereas the third treatment plant had an advanced membrane bioreactor treatment (MBR). All of the analytes in the study, including (nonylphenol (NP), octylphenol (OP), and some of their ethoxylated chains AP_nEOs (n ≤ 7), 17β-estradiol (E2), estriol (E3), 17α-ethynylestradiol (EE) and bisphenol-A (BPA)), were found in almost all samples in concentrations ranging from 0.9 to 710.2 ng g⁻¹.     

Determination of endocrine-disrupting compounds in cereals by pressurized liquid extraction and liquid chromatography-mass spectrometry. Study of background contamination

A sensitive method based on pressurized liquid extraction (PLE) and liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) has been developed for the determination in cereal samples of seven endocrine-disrupting compounds: bisphenol A (BPA), 4-tert-butylbenzoic acid (BBA), 4-nonylphenol (NP), 4-tert-butylphenol (t-BP), 2,4-dichlorophenol (DCP), 2,4,5-trichlorophenol (TCP) and pentachlorophenol (PCP). For the PLE procedure, methanol was selected as the extraction solvent. An experimental design approach was applied to optimize other PLE parameters. The recoveries achieved for the all seven compounds were in the 81-104% range, with relative standard deviations of 4-9%. An additional preconcentration step, based on solid-phase extraction (SPE), after the PLE step proved to be a successful way for obtaining a more sensitive method. The detection limits achieved in corn breakfast cereals were in the 0.003-0.013 microg g(-1) range, except for BPA, with a detection limit of 0.043 microg g(-1), for a sample size of 2.5 g. These values are similar to or even lower than currently legislated limits for pesticides in cereals and cereal-based foodstuffs. We also investigated possible contamination during the experimental process by the target compounds released from purified water, plastics, syringes, peristaltic pump tubes, glassware and other laboratory materials in contact with the samples along the analytical process.     

人体尿液中双酚A与壬基酚的同位素稀释的LC-MS/MS分析

采用固相萃取、高效液相色谱-质谱/质谱仪同位素稀释技术,建立了尿中双酚A(BPA)和壬基酚(NP)的检测方法.液相色谱分离用C18柱,流动相为甲醇和水,负离子电喷雾模式电离,多反应离子监测方式检测,BPA和NP的定量离子对分别为m/z 226.9/132.9、219.3/118.8, 13C和氘代同位素化合物分别做回收率控制和内标定量.BPA和NP的基质加标回收率分别为86% ～106%、90% ～114%,定量下限分别为0.97、2.3 μg/L.实际尿样的测定验证了该方法的可行性.     

Simultaneous determination of tetrabromobisphenol A, tetrachlorobisphenol A, bisphenol A and other halogenated analogues in sediment and sludge by high performance liquid chromatography-electrospray tandem mass spectrometry

A high performance liquid chromatography-electrospray (negative) ionization-tandem mass spectrometry (HPLC-ESI(-)-MS-MS) based method has been developed for simultaneous determination of bisphenol A (BPA), tetrachlorobisphenol A (TCBPA), and tetrabromobisphenol A (TBBPA), as well as lower brominated BPA analogues in sediment and sludge samples. Samples were extracted with MTBE, target compounds were partitioned by aqueous solution of sodium hydroxide. The solution was subsequently acidified, and the enrichment and desalting were performed via solid phase extraction (SPE). After cleanup the target compounds were determined by HPLC-ESI(-)-MS-MS. The method limits of quantification (MLOQs) from sediment and sludge for BPA, monobromo-bisphenol A (mono-BBPA), dibromo-bisphenol A (di-BBPA), tribromo-bisphenol A (tri-BBPA), TBBPA and TCBPA were 0.15, 0.02, 0.02, 0.04, 0.05, and 0.03 ng/g (dry weight), respectively. Mean recovery of the analytes from spiked samples ranged from 70 to 105%, and the relative standard deviation ranged from 4.9 to 13.1%. The method was successfully applied to sediment and sludge samples analysis.     

Analysis of Six Phenolic Endocrine Disrupting Chemicals in Surface Water and Sediment

An efficient and reliable method based on gas chromatography–mass spectrometry (GC–MS) was developed for the extraction and analysis of six phenolic endocrine disrupting chemicals (EDCs), such as 4-nonylphenol (4-NP), nonylphenol-mono-ethoxylate (NP1EO), nonylphenol-di-ethoxylate (NP2EO), 4-tert-octylphenol (4-t-OP), bisphenol A (BPA) and 4-cumylphenol (4-CP) in surface water and sediment. The method was developed by using microwave-assisted extraction (MAE), solid phase extraction (SPE) and derivatization procedure. The MAE procedures were performed by optimizing three key process factors, consisted of extraction solvent, extraction temperature and holding time, affecting the extraction efficiency from sediment samples. For SPE, various parameters that may affect the recovery efficiency of water samples, such as SPE phase cartridge, elution solvent, as well as pH of water samples, were investigated. A series of derivatization conditions, such as derivatization reagent, reaction temperature and reaction time, were improved. The method achieved good repeatability and reproducibility with relative standard deviations <13% for all target EDCs in the both samples. Satisfactory recoveries for spiked water and sediment samples ranged from 85 to 101% and 74 to 105%, respectively. The limits of quantification varied from 0.20 (4-t-OP) to 11.50 ng L^?1 (NP2EO) and from 0.31 (4-t-OP) to 9.50 ng g^?1 dry weight (dw) (NP2EO) for water samples and sediment samples, respectively. The established method was successfully applied to the analysis of target EDCs in surface water and sediment samples collected from Caohai site of Dianchi Lake, China. The results showed that NP1EO, NP2EO and BPA were the three dominant phenolic EDCs in the site, reaching 114, 97 and 149 ng L^?1 in surface water, while 444, 186 and 178 ng g^?1 dw in surface sediment, respectively.     

Comparison of electrospray ionization,atmospheric pressure chemical ionization and atmospheric pressure photoionization for determining estrogenic chemicals in water by liquid chromatography tandem mass spectrometry with chemical derivatizations

This study compared the sensitivities and matrix effects of four ionization modes and four reversed-phase liquid chromatographic (LC) systems on analyzing estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), 4-nonylphenol (NP), 4-tert-octylphenol (OP), bisphenol A (BPA) and their derivatives of dansyl chloride or pentafluorobenzyl bromide (PFBBr) in water matrixes using a triple-quadrupole mass spectrometer with selected reaction monitoring (SRM). The four probes were electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI) and APCI/APPI; the four LC systems were ultra-performance liquid chromatography (UPLC) with or without post-column split, a mixed-mode column and two-dimensional LC (2D-LC). Dansylated compounds with ESI at UPLC condition had the most intense signals and less matrix effects of the various combinations of ionization and LC systems. The on-column limits of detection (LODs) of dansylated estrogens by SRM were 0.05–0.20 pg, and the LODs in sewage treatment plant effluent and in river water were 0.23–0.52 and 0.56–0.91 ng/L, respectively. The LODs using selected ion monitoring (SIM) reached low ng/L levels in real samples and measured concentrations were comparable with those of SRM.     

高效液相色谱法测定饮料类食品中的类雌激素

利用OASIS HLB固相萃取柱富集和净化样品,采用高效液相色谱分离,紫外及荧光检测,建立了不同饮料基质中的壬基酚(NP)、辛基酚(OP)和双酚A(BPA)等类雌激素的测定方法,并用该方法对市售部分产品进行了测定。不同加标水平的BPA,NP和OP的回收率为91.08%~103.19%,其相对标准偏差为0.5%~5.49%。该方法具有操作简单、灵敏度高和重现性好等优点。