Polymer-supported 1,4-dihydronicotinamide: Synthesis and Application in the Reduction of the Activated Olefins

s: Three new polymer-supported NAD(P)H models (I, II, III) were designed and synthesized, which can efficiently reduce many activated olefins under mild conditions.1 The most advantageous feature of the three NAD(P)H models is (i) easy work-up and separation of the reaction products and (ii) good potential for recycle use of the NAD(P)H models, which makes the three new polymer-supported NAD(P)H models a promising alternative both in research laboratories and in industrial processes.…     

ELECTRON MICROSCOPIC AUTORADIOGRAPHIC STUDIES ON DYNAMICS AND LOCATION OF DNA SYNTHESIS OF DUCK PLAGUE VIRUS

Electron microscopic autoradiographic studies on the dynamics and location of DNAsynthesis by means of incorporation of ~8H-thymidine during the replication of duck plaguevirus (DPV) revealed that the duration of DNA synthesis of DPV was rather long. The repli-cation of viral DNA occurred simultaneously with the assembly procedure of nucleocapsids, thematuration and release of viruses. DNA synthesis of DPV occurred in the matrix with lowerelectron density in the nucleus. The replicated viral DNA accumulated in the viroplast With highelectron density where the assembly of nucleocapsids occurred. The viroplast with high electrondensity was not the region of viral DNA synthesis.     

Detection of dopamine using self-assembled diazoresin/single-walled carbon nanotube modified electrodes

Ultrathin films of diazoresin(DR)/single-walled carbon nanotube(SWNT) were fabricated on thioglycollic acid(TGA) decorated gold(Au) electrodes by the self-assembly method combined with the photocrosslinlcing technique.The electrochemical behavior of dopamine(DA) at the DR/SWNT modified electrodes was studied using the cyclic voltammetry(CV) and differential pulse voltammetry(DPV) methods.Under the optimal conditions,a linear CV response to DA concentration from 1 μmol/L to 40 μmol/L was observed,and the detection limit of DA was 2.1 ×10~(-3) μmol/L via the DPV method in the presence of 10 μmol/L of uric acid(UA) or 2.5 × 10~(-3) μmol/L via the DPV method in the presence of10 μmol/L of ascorbic acid(AA).Moreover,the modified electrodes exhibited good reproducibility and sensitivity,demonstrating its feasibility for analytical purposes.     

Synthesis of NAD analogs to develop bioorthogonal redox system

Three new nicotinamide adenine dinucleotide(NAD) analogs were synthesized,and their characteristics as cofactors for Escherichia coli malic enzyme(ME) and its double mutant ME L310R/Q401C were analyzed.Each pair of the NAD analog and the double mutant showed good orthogonality to the natural pair of NAD and ME in terms of catalyzing oxidative decarboxylation of L-malic acid.Results indicated that molecular interactions between redox enzyme and cofactor could be further explored to generate new bioorthogonal redox systems.     

Study on the hydrogenation coupling of methane

At atmospheric pressure and ambient temperature, the hydrogenation coupling of methane was studied by using pulse corona plasma and its synergism with catalyst. The results showed that (i) under pulse corona plasma, the coupling of methane could be fulfilled by the addition of hydrogen, and with the increase of the amount of hydrogen, the conversion of methane and the yield of C2 hydrocarbon increased, and the deposit of carbon decreased; (ii) the conversion of methane was affected by pulse voltage and repeated frequency; (iii) in the system, the addition of Ni/y-AI203 could improve the distribution of C2 hydrocarbon; (iv) the activity of Ni/y-AI2O3 prepared by cold plasma was better than that by chemical methods. The experiment opened up a new technical route of the coupling of methane.     

An electrochemical aptasensor based on cu2+-induced signal amplification strategy for the detection of ochratoxin aEI北大核心CSCD

A novel electrochemical aptasensor based on a Cu2+-induced signal amplification strategy was constructed for the rapid, sensitive and specific detection of ochratoxin A （OTA）. The OTA aptamer with poly （T） was hybridized with the captured DNA probe on the electrode surface. In the presence of Cu2+ and ascorbic acid, the end of poly（T） was used as a template to in situ grow copper nanoclusters （Cu NCs）. In the absence of targeted OTA, the gold electrodes after decorating Cu NCs were immersed into an acidic environment to release Cu2+. After enriching Cu2+ at a potential of − 1.6 V, the strongest current value of copper was recorded by measuring differential pulse voltammetry （DPV）. In the presence of OTA, the OTA aptamer was tightly bound to the target OTA. The OTA aptamer broke away from the electrode to reduce the growth of Cu NCs, resulting in lower DPV current response. This proposed method was employed to detect OTA with linear range from 0.1 to 50.0 ng/mL, and the detection limit was 41.2 pg/mL. The Cu2+-induced electrochemical aptasensor can be further applied in the analysis of target OTA in coffee solution samples. © 2023, Youke Publishing Co.,Ltd. All rights reserved.     

基于纳米结构的电化学传感器同时测定溶液中异丙肾上腺素、尿酸和叶酸的浓度(英文)

A carbon paste electrode modified with 2‐((7‐(2,5-dihydrobenzylideneamino)heptylimino)methyl) benzene‐1,4‐diol(DHB) and carbon nanotubes were used to simultaneously determine the concen-trations of isoproterenol(IP), uric acid(UA), and folic acid(FA) in solution. First, cyclic voltammetry was used to investigate the redox properties of the modified electrode at various scan rates. Next, the mediated oxidation of IP at the modified electrode is described. At the optimum pH of 7.0, the oxidation of IP occurs at a potential about 90 mV less than that of an unmodified carbon paste elec-trode. Based on the results of differential pulse voltammetry(DPV), the oxidation of IP showed a dynamic range between 10 and 6000 μmol/L, and a detection limit of 1.24 μmol/L. Finally, DPV was used to simultaneously determine the concentrations of IP, UA, and FA in solution at the modified electrode.     

Temperature effect on the ease of aggregation

The effect of temperature on the critical aggregate concentration (CAgC) has been investigated for the first time, by measurement of the hydrolytic rate constants of p-nitrophenyl dodecanoate (C12) and hexadecanoate (C16) at different temperatures in the 30:70 V/V (Φ=0.30) and 40:60 V/V (Φ= 0.40) dioxane (DX)-H2O systems. The CAgC values of C12 and C16 increase with the increase of the temperature, i.e., high temperatures disfavor aggregation. Activation energies for the hydrolysis of C12 in the monomeric and aggregated concentration domains have also been discussed.     

Electrocatalytic Oxidation of NADH Based on Self-assembled Colloidal Gold and Nafion Matrixes and Co Complex Mediator

Nicotinamide adenine dinucleotide (NAD ) and its reduced form NADH used as coenzymes by over 300 dehydrogenases have been the subjects of numerous studies. Direct oxidation of NADH at a bare electrode only proceeds at high overpotentials, which finally le…     

Action of the Selenomorpholine Compounds on the Bacterium Growth by Microcalorimetry

The action of β-(N-selenomorpholine) ethyl phenyl ketone hydrochloride and 4-(N-selenomorpholine)-2-butanone hydro-chloride on Escherichia coli and Staphylococcus aureus was studied by microcalorimetry,Differences in their capacities to affect the metabolism of this bacterium were observed.The kinetics shows that the selenomorpholine compounds had action on the metabolism process of Escherichia coli and Staphylococcus aureus.The rate constant (k) of the studied bacterium in the presence of the drugs are concentration-dependant.The growth rate constants decrease with an increase in the mass of the selenomorpholine compounds ,but their relationship is different.As deduced from the rate constant(k) of the studied bacterium(in log phase )and the half inhibitory concentration (IC50),the experimental results reveal that the studied selenomorpholine compounds all have good antibiotic activity and better antibacterial activity on Staphylcoccus aureus than on Escherichia coli.     

A spectral study of the interaction between chelerythrine chloride and cytidine

To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV-vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculations. The binding of CHE to cytidine could be characterized by the hypochromic and bathochromic effects in the absorption bands, and the quenching of fluorescence intensity. The spectral data were fit by linear analysis, yielding a binding constant of 2.49×104 L mol-1 at 25℃of CHE and cytidine, and a van't Hoff enthalpy of -20.02 kJ/mol for the exothermic interaction in the standard state. In addition, with