Laser-Induced Fluorescence Bronchoscopy for Detection and Localization of Early Lung Cancer

Experimenatal results on the development of a Laser-Induced Fluorescence Bronchoscopy(LIFB) for the detection and localization of early lung cancer are reported in this paper. The system utilizes fluorescence of photosensitizer drug to provide real time video imaging for the examined lung tissue. Color filters are used to differentiate signal from background and a computer image processing technique is also applied to subtract the background. Moreover, a pseudocolor contrast enhancement method was developed to enhance the fluorescence image displayed on the vidio monitor. Suspicious areas are identified by pseudocolor image to guide biopsy, and several clinical trials show that sensitivity and contrast capability of the system should permit the detection and localization of early lung cancer.     

肺癌荧光图象的伪彩色增强处理

近年来,作者开发了肿瘤荧光成象法用于诊断和定位早期支气管癌.由于所获得的荧光图象使用了象增强器进行增益放大,因此还消除了肺癌荧光图象的彩色信息.本文给出了一种荧光图象伪彩色增强处理的方法,并证实了伪彩色增强技术用于早期肺癌荧光诊断的可行性.经增强处理后,内镜医生只要根据彩色视频图象中与黑白荧光图象强度相对应的颜色就可以鉴别肺癌存在与否.同增强处理前的黑白图象相比,医生从伪彩色图象中获得了病灶内部结构、边缘效果和立体感方面的更多信息.临床结果表明可疑组织与正常组织之间获得了足够高的颜色衬比,有助于诊断早期肺癌病灶.     

具有图象处理功能的激光荧光内窥系统的研究

内窥镜荧光图象系统是体腔内早期肿瘤诊断和定位的有效手段.但是早期开发研究的LFE荧光成家系统由于存在假阳性和假阴性误诊而限制了它的广泛开展和应用.本文仔细探讨了该系统产生误诊的原因,并在此基础上提出了使用计算机图象处理技术的荧光图象系统,而且通过实验验证了这种新技术,它能有效地克服假阳性和假阴性误诊,为体腔内肿瘤的诊断提供可靠判据.     

Hyaluronan functionalizing QDs as turn-on fluorescent probe for targeted recognition CD44 receptor

The recognition of tumor markers in living cancer cells has attracted increasing interest. In the present study, the turn-on fluorescence probe was designed based on the fluorescence of thiolated chitosan-coated CdTe QDs (CdTe/TCS QDs) quenched by hyaluronan, which could provide the low background signal for sensitive cellular imaging. This system is expected to offer specific recognition of CD44 receptor over other substances owing to the specific affinity of hyaluronan and CD44 receptor (~8–9 kcal/mol). The probe is stable in aqueous and has little toxicity to living cells; thus, it can be utilized for targeted cancer cell imaging. The living lung cancer cell imaging experiments further demonstrate its value in recognizing cell-surface CD44 receptor with turn-on mode. In addition, the probe can be used to recognize and differentiate the subtypes of lung cancer cells based on the difference of CD44 expression on the surface of lung cancer cells. And, the western blot test further confirmed that the expression level of the CD44 receptor in lung cancer cells is different. Therefore, this probe may be potentially applied in recognizing lung cancer cells with higher contrast and sensitivity and provide new tools for cancer prognosis and therapy.

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Graphical abstract ? Hyaluronan functionalizing sulfhydryl chitosan-coated CdTe QDs were designed as a “turn-on” fluorescence probe for recognizing cancer cells. ? The fabricating probe is based on the high affinity of hyaluronan and CD44 receptor for specific recognition of CD44 receptor in lung cancer cell. ? The probe can be used to recognize and differentiate the subtypes of lung cancer cells based on the difference of CD44 expression on the surface of lung cancer cells. ? The probe showed the lower background signal than that of always-on nanoprobes avoiding the non-specific adsorption on the surface of the normal cells.

     

An improved infrared dim and small target detection algorithm based on the contrast mechanism of human visual system

To achieve higher detection rate and lower false alarm rate in dim and small target detection, this paper proposed an improved algorithm based on the contrast mechanism of human visual system (HVS) for infrared small target detection in an image with complicated background. According to the contrast mechanism of HVS, Laplacian of Gaussian (LoG) filter is exploited to deal with the input image, which can not only suppress the background noise and clutter but also enhances the target intensity significantly. As a result it increases the contrast ratio between target and background. To further eliminate residual clutter, we process the filtered image with morphological method in all directions. True target is finally obtained by applying local thresholding segmentation to the pre-processed image. Experimental results demonstrate its superior and reliable detection performance by high detection rate and low false alarm rate.     

基于均匀颜色知觉空间的多光谱图像彩色合成

本文在分析现有多光谱图像彩色合成方法的基础上，提出了基于均匀颜色知觉空间的ＨＬＣ合成法，讨论了单谱段图像映射、分量图像合成、色域变换以及合成图像的色差预测和评估、彩色变换和逆变换，分析了影响合成图像视觉效果的因素。应用于伪装目标的识别获得了比ＲＧＢ、ＹＭＣ、ＩＨＳ等合成法更好的效果     

Metabolism-enhanced tumor localization by fluorescence imaging: in vivo animal studies

We present a high-sensitivity near-infrared optical imaging system for noninvasive cancer detection and localization based on molecularly labeled fluorescent contrast agents. This frequency-domain system utilizes the interferencelike pattern of diffuse photon density waves to achieve high detection sensitivity and localization accuracy for the fluorescent heterogeneity embedded inside the scattering media. A two-dimensional localization map is obtained through reflectance probe geometry and goniometric reconstruction. In vivo measurements with a tumor-bearing mouse model by use of the novel Cypate-mono-2-deoxy-glucose fluorescent contrast agent, which targets the enhanced tumor glycolysis, demonstrate the feasibility of detection of a 2-cm-deep subsurface tumor in the tissuelike medium, with a localization accuracy within 2-3 mm.     

基于PDE的前视红外成像舰船目标检测

针对复杂环境下的海面目标提取问题,提出一种基于偏微分方程（PDE）的红外舰船检测算法。首先采用基于PDE理论的滤波模型对初始图像滤噪并进行背景估计,然后结合邻域差分进行背景抑制后分割提取目标。实验表明,该算法能够有效检测强杂波背景中的目标,方法适应性强。     

Prediction of the performance of an algorithm for the detection of small targets in infrared images

Detection of small targets in infrared (IR) images is important in IR image processing. For the prediction of performance of a detection algorithm, it is necessary to calculate the probability of detection and probability of false alarm. A method is developed to calculate the probabilities in this paper. The detection is divided into two parts: the first part, which is called pre-detection, is to find out candidates for targets in a single frame of an image; and the second part is to localize the target in multiple frames of the image. Under some assumptions, the pre-detection probability, the false detection probability of single frame, detection probability and false alarm probability are derived. The algorithm for the detection of small target in IR image, which is used for the derivation of the probabilities, is contrast threshold detection based on background prediction, and a pipeline filter is used for multiframe image processing. The results show the relationship of the probabilities to the contrast of target to background, SNR, and contrast threshold.     

Cross-talk reduction in confocal images of dual fluorescence labelled cell spheroids.

Dual fluorescence labelling is an advanced method to separate two individual specimens in a biological system using confocal microscopy. An inherent problem of this method is fluorescence channel cross-talk, which causes problems for the exact spatial determination and separation of the specimens. Using a parallel fluorescence detection and an image processing technique, based on an image subtraction method, we have developed a very straight forward method for correcting the dual channel fluorescence images. We successfully applied this method to a 3-dimensional cancer spheroid invasion assay and controlled the cross-talk compensation efficiency by a quality parameter.     

Phase modulation pseudocolor encoding ghost imaging

We present a ghost imaging scheme that can obtain a good pseudocolor image of black-and-white objects.The essential idea is to use a multi-wavelength thermal light source and the phase modulation pseudocolor encoding technique,which overcomes the disadvantages of other methods involved spatial filtering.Therefore,the pseudocolor ghost image achieved by this imaging scheme is better than that obtained by other methods in terms of brightness,color,and signal-tonoise ratio.     

Frequency-domain fluorescence lifetime imaging for endoscopic clinical cancer photodetection: Apparatus design and preliminary results

We describe a new fluorescence imaging device for clinical cancer photodetection in hollow organs in which the tumor/normal tissue contrast is derived from the fluorescence lifetime of endogenous or exogenous fluorochromes. This fluorescence lifetime contrast gives information about the physicochemical properties of the environment which are different between normal and certain diseased tissues. The excitation light from a CW laser is modulated in amplitude at a radio frequency by an electrooptical modulator and delivered by an optical fiber through an endoscope to the hollow organ. The image of the tissue collected by the endoscope is separated in two spectral windows, one being the backscattered excitation light and the other the fluorescence of the fluorochrome. Each image is then focused on the photocathode of image intensifiers (II) whose optical gain is modulated at the same frequency as the excitation intensity, resulting in homodyne phase-sensitive images. By acquiring stationary phase-sensitive frames at different phases between the excitation and the detection, it is possible to calculate in quasi-real time the apparent fluorescence lifetime of the corresponding tissue region for each pixel. A result obtained by investigating the endogenous fluorochromes present in the mucous membrane of an excised human bladder is presented to illustrate this method and most of the optical parameters which are of major importance for this photodetection modality have been evaluated.     

利用全息元件处理大面积图象

本文提出了一种专门制作的全息元件作照明器的图象处理系统,它可以利用白光照明实时处理大面积图象。这种系统可以进行两张图象差异的彩色显示、黑白图象的假彩色编码和彩色合成,以及对体视对图片的体视观察等。本文最后给出了实验结果。     

基于小波分析和伪彩色处理的低对比度图像增强

提出了一种对低对比度图像进行增强处理的新技术。在对传统的伪彩色增强技术改进的基础上,将小波分析和伪彩色两种方法有机地相结合,从而克服了这两种方法的缺陷,获得了良好的增强效果。同时对实现低对比度图像增强的基本方法和基本步骤进行了详细的阐述,并将该技术应用到透视图像分析中。实验结果证明了该方法的可行性,该方法为低对比度图像增强处理提供了一种新途径。     

用于探测生物芯片的制冷型ICCD系统

利用光锥耦合的ICCD系统探测荧光染料标记的生物芯片,并对CCD芯片和像增强器制冷,以提高探测灵敏度。基于实验分析结果,指出背景噪声的主要来源为杂散光和生物芯片基底所发的荧光,指出用镜头成像限制了系统探测灵敏度的提高,可采用低荧光物质作为生物芯片的基底对系统加以改进。     

Resolution enhancement of random adsorbed single-molecule localization based on surface plasmon resonance illumination

Single-molecule localization (SML) is a powerful tool to overcome the diffraction limit in optical imaging, because the fluorescence emitted by single molecules can be observed with nanometer accuracy when the optical background and associated noise are made sufficiently small. Random adsorbed SML has been successfully demonstrated for superresolution imaging on metal surfaces. To optimize the random adsorbed SML, we developed a new illumination method based on surface plasmon resonance (SPR). The enhancement of the fluorescence signal and the reduction of background noise were achieved simultaneously. A high localization resolution of 15?nm was demonstrated with this new SPR illumination system.     

早期乳腺肿瘤的超宽带微波稳健波束形成成像检测系统

提出了一种基于自主设计小型超宽带天线的微波稳健波束形成(RCB)成像肿瘤检测系统.仿真结果表明,该检测系统对肿瘤反射信号有很高的敏感度.在简单平面模型和核磁共振成像图(MRI)导出模型中进行仿真检测实验,并将天线阵列接收信号用RCB算法进行成像处理.从乳房重构图像中能够得到正确的肿瘤位置及大小信息,实现了平面模型中最小直径3 mm和MRI导出模型中最小直径4 mm的肿瘤检测,证实了该检测系统用于早期乳腺肿瘤检测的可行性.     

基于多帧背景的泄漏气体自适应匹配滤波检测

被动傅里叶变换红外(FTIR)扫描遥测成像系统采集的红外高光谱图像具有空间、光谱等维度信息,可被用于大气环境中有毒有害气体的识别、定量及可视化。该系统具有光谱分辨率高、非接触式及远距离探测等优点,然而其单帧图像的像元数量少且部分存在气体吸收或发射特征,无法直接用于红外高光谱图像的目标检测。提出了基于多帧背景的泄漏气体自适应匹配滤波(AMF)检测方法,以短时间内、同一区域的多帧红外高光谱图像为基础,筛选出无目标气体特征的背景光谱并计算探测区域的背景最大似然估计,应用于后续帧的目标气体泄漏检测。红外高光谱图像来自于SF₆气体的遥测实验,共扫描四帧（120像元/帧）,去除前三帧内含有目标气体特征的像元光谱,剩余背景光谱被用于计算背景的最大似然估计,第四帧红外高光谱图像逐像元对SF₆气体进行的AMF检测,并与非线性最小二乘法反演的SF₆柱浓度图像比对,结果表明AMF检测高值与柱浓度高值有较强的相关性。为验证多帧背景在不同空间检测方法下的性能,分别对该帧数据进行了基于正交子空间的自适应子空间检测(ASD)、基于混合空间的自适应余弦检测(ACE)及基于斜子空间的最大似然比检测(OGLRT),并分别与SF₆柱浓度图像比对,结果表明多帧背景适用于不同空间的检测方法。此外,为验证存在目标气体吸收特征的非背景光谱对背景空间的影响,向背景空间中加入多条含有SF₆气体吸收特征的光谱,通过ROC曲线检验,结果表明背景空间中混入目标气体特征会降低AMF方法的检测性能。AMF检测值的假彩色图像也能应用于被动FTIR扫描遥测成像系统,相较于柱浓度假彩色图像,泄漏源及扩散趋势更为明显。基于红外高光谱图像的检测方法依赖于整体背景的统计特性,相较于单像元光谱波段的反演算法,极大地降低了背景的依赖性。多帧背景下的AMF泄漏气体检测方法能很好地应用于被动FTIR扫描遥测成像系统上并满足在线监测要求。     

Study of Blood Porphyrin Spectral Profile for Diagnosis of Tumor Progression

Renal cell carcinoma (RCC) accounts for approximately 3% of new cancer incidence and mortality in the United States. Unfortunately many RCC masses remain asymptomatic and nonpalpable until they are advanced. Diagnosis and localization of early carcinoma play an important role in the prevention and curative treatment of RCC. The autofluorescence of blood porphyrin of healthy and tumor induced in male SCID mice was analyzed using fluorescence and excitation spectroscopy. A significant contrast between normal and tumor blood could be established. Blood porphyrin fluorophore showed enhanced fluorescence band (around 630 nm) in function of the tumor growth. This indicates that either the autofluorescence intensity of the blood fluorescence may provide a good parameter for the “first approximation” characterization of the tumor stage.     

Intrinsic Fluorescence of Protoporphyrin IX from Blood Samples Can Yield Information on the Growth of Prostate Tumours

Prostate cancer is one of the most common types of cancer in men, and unfortunately many prostate tumours remain asymptomatic until they reach advanced stages. Diagnosis is typically performed through Prostate-Specific Antigen (PSA) quantification, Digital Rectal Examination (DRE) and Transrectal Ultrasonography (TU). The antigen (PSA) is secreted by all prostatic epithelial cells and not exclusively by cancerous ones, so its concentration also increases in the presence of other prostatic diseases. DRE and TU are not reliable for early detection, when histological analysis of prostate tissue obtained from a biopsy is necessary. In this context, fluorescence techniques are very important for the diagnosis of cancer. In this paper we explore the potential of using endogenous phorphyrin blood fluorescence as tumour marker for prostate cancer. Substances such as porphyrin derivatives accumulate substantially more in tumours than in normal tissues; thus, measuring blood porphyrin concentration by autofluorescence intensity may provide a good parameter for determining tumour stage. In this study, the autofluorescence of blood porphyrin was analyzed using fluorescence and excitation spectroscopy on healthy male NUDE mice and in those with prostate cancer induced by inoculation of DU145 cells. A significant contrast between the blood of normal and cancer subjects could be established. Blood porphyrin fluorophore showed an enhancement on the fluorescence band around 632 nm following tumour growth. Fluorescence detection has advantages over other light-based investigation methods: high sensitivity, high speed and safety. However it does carry the drawback of low specificity of detection. The extraction of blood porphyrin using acetone can solve this problem, since optical excitation of further molecular species can be excluded, and light scattering from blood samples is negligible.