毛细管阵列电泳与规模化DNA测序

 根据 10 0 80份基因组DNA测序的结果 ,讨论了毛细管阵列电泳测序方法的技术特点 ,并对影响测序结果的一些因素进行了分析。在此基础上与平板凝胶电泳方法进行比较 ,显示了毛细管阵列电泳的优点。同时也对大规模测序技术环节之间的协调进行了探讨 。     

用于毛细管电泳分离DNA的聚合物介质的研究进展

综述了用于毛细管电泳分离DNA及测序的聚合物介质的研究进展。这类聚合物主要有均聚物、无规共聚物、嵌段共聚物、接枝共聚物、共混聚合物、准互穿聚合物网络和微交联纳米凝胶聚合物,并对各种结构的聚合物的筛分性能进行了比较。     

DNA Sequencing by Capillary Electrophoresis Using Quasi-interpenetrating Network Formed by Polyacrylamide and Poly（N-hydroxymethylacrylamide）

Capillary electrophoresis (CE) in conjunction with laser induced fluorescence (LIF) is a powerful tool for DNA sequencing1. A number of water-soluble polymers have been used for this purpose. We have recently reviewed studies on separation medium on DNA s…     

A Novel Method for Quantification of Circulating DNA in Serum by Capillary Zone Electrophoresis

In this paper, we first presented a novel method for quantification of circulating DNA in human serum based on capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). The serum was digested by proteinase to release free DNA, and then CZE-LIF system was used for the quantification of total circulating DNA. This method was successfully used to quantify the circulating DNA levels in sera from healthy individuals and certain cancer patients.We found the significantly elevated circulating DNA levels in certain prostate cancer patients. Our results demonstrated that CZE-LIF system has good linearity, excellent sensitivity (0.5 ng/mL DNA),satisfactory reproducibility (RSDs in one day and between days were both less than 5%) and reliability, and is well suitable to the quantification of the circulating DNA in human serum or plasma.     

Evaluation of The Interaction Between Netropsin and Double Stranded DNA by Capillary Zone Electrophoresis

DNAmoleculesarepolymorphic,andexistinavarietyofstructuralformsthatmightprovideuniquebindingsitesforsmallmolecules1.ManysmallmoleculesthatinteractwithDNAhavepotentialtherapeuticapplicationsininhibitingtumorgrowth,andviral,bacterial,fungalandparasiticinfections.Thesedrugscanbedividedintotwocategories:intercalatorandminorgroovebinder2-3.SoDNA-druginteractionsareaparticularlyimportantclassofintermolecularinteractions.ManymethodshavebeenperformedtostudythebasisofDNA-druginteractions4.However,i…     

样品稀释对毛细管电泳分离检测DNA片段的影响

通过理论推导和实验验证表明;适当稀释DNA样品溶液,采用流体力学进样或电动进样都不会较大地减低峰高,而DNA片段毛细管电泳的分离效率和分离度还能有所提高。采用稀释样品的方法可提高DNA样品的使用效率。采用羟乙基纤维素无胶筛分介质分离了DNA片段。用激光诱导荧光（氩离子激光器,488nm）电荷耦合器件检测。用低浓度的筛分介质（0．4％）分离了分子质量较大的ADNA－HindⅢ全部8个片段（12bp～23130bP）。用高浓度的筛分介质（1．6％）分离分子质量较小的pBR322－HaeⅢ22个片段（18bp～587bp）。     

市售保健品中7种醒酒护肝功效成分的毛细管电泳测定

采用胶束电动毛细管电泳-二极管阵列检测器同时检测了市售保健品中儿茶素、表儿茶素、表没食子儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯、二氢杨梅素和甘草酸等7种醒酒护肝功效成分.采用正交设计法对毛细管电泳条件[缓冲剂浓度、添加剂十二烷基硫酸钠(SDS)浓度、添加剂乙腈比例以及电泳缓冲溶液p H]进行了优化.在优化的条件下,7种组分在各自的线性范围内相关系数r≥0.9989,检出限和定量限分别为0.26~2.22μg/g(S/N=3)和0.87~7.39μg/g(S/N=10),日内精密度和日间精密度分别为1.3%~2.5%和1.9%~3.9%,样品加标回收率为91.4%~104.9%,加标样品的相对标准偏差(RSD)在1.4%~3.2%之间.本方法可在8 min内实现7种功效成分的同时检测,能够满足市售醒酒护肝产品的常规分析和质量评价要求.     

羟乙基纤维素及蔗糖无胶筛分毛细管电泳分离DNA片段

提出以羟乙基纤维素、蔗糖和硼酸形成“互穿网络”无胶筛分介质，显著地提高了分离效率，分离了φＸ１７４／ＨａｅⅢＤＮＡ的所有１１个ＤＮＡ片段，探讨了“互穿网络”无胶筛分机理。将该方法应有和于决定鳝鱼性别基因ＰＣＲ扩增产物的分离与鉴定，结果较好。     

The Use of Dual Cyclodextrin Chiral Selector Systems in the Enantioseparation of Pharmaceuticals by Capillary Electrophoresis: An Overview

Cyclodextrin (CD) derivatives are the most efficient and frequently used chiral selectors (CSs) in capillary electrophoresis (CE). There are situations when the use of a single CD as CS is not enough to obtain efficient chiral discrimination of the enantiomers; in these cases, sometimes this problem can be resolved using a dual CD system. The use of dual CD systems can often dramatically enhance enantioseparation selectivity and can be applied for the separation of many analytes of pharmaceutical interest for which enantioseparation by CE with another CS systems can be problematic. Usually in a dual CD system an anionic CD is used together with a neutral one, but there are situations when the use of a cationic CD with a neutral one or the use of two neutral CDs or even two ionized CDs can be an efficient solution. In the current review we present general aspects of the use of dual CD systems in the analysis of pharmaceutical substances. Several examples of applications of the use of dual CD systems in the analysis of pharmaceuticals are selected and discussed. Theoretical aspects regarding the separation of enantiomers through simultaneous interaction with the two CSs are also explained. Finally, advantages, disadvantages, potential and new direction in this chiral analysis field are highlighted.     

Rapid Capillary Electrophoresis Method for Simultaneous Determination of Abemaciclib,Ribociclib, and Palbociclib in Pharmaceutical Dosage Forms: A Green Approach

Advances in the treatment of HR+/HER2- breast cancer phenotype have been made with the introduction of abemaciclib, ribociclib, and palbociclib, inhibitors of cyclin D dependent kinases 4 and 6 (CDK4/6). Here, a novel, fast, cheap, and green CE method for the simultaneous determination of these three CDK4/6 inhibitors in less than 4 min is proposed for the first time. Separation was achieved by capillary zone electrophoresis in an acidic medium, in accordance with the structures of the analytes and their pKa values. The optimal pH of the running buffer was found to be 2.9. The optimal method conditions were 27.5 kV separation voltage, 30 °C, 5 s injection time under 50 mbar pressure, and 50 mM phosphate background buffer with benzimidazole as an internal standard. The developed method was validated with respect to robustness, selectivity, accuracy, precision, linearity, and limits of detection. The method was shown to be linear in the range of 10 to 100 µg mL⁻¹ with correlation coefficients higher than 0.9981. A greenness assessment of the proposed method was performed, and the method was shown to be green. The validated method was successfully applied to pharmaceutical dosage forms of all CDK4/6 inhibitors.