共查询到20条相似文献,搜索用时 31 毫秒
1.
K. Watanabe H. Hattori M. Nishikawa A. Ishii T. Kumazawa H. Seno O. Suzuki 《Chromatographia》1997,44(1-2):55-58
Summary Cocaethylene together with cocaine spiked in human whole blood has been found measurable at high sensitivities by capillary
gas chromatography with surface ionization detection. The drugs could be rapidly extracted by Sep-Pak C18 cartridges with recovery of more than 60%. The calibration curves for both cocaethylene and cocaine using cocapropylene as
internal standard were linear in the range 50–300 pmol mL−1 of whole blood. The detection limits of cocaethylene and cocaine were 5–10 pmol mL−1 (0.1–0.2 pmol on column if recovery is 100%). Cocaethylene could be determined for whole blood obtained from rats (ca. 200
g body wt.), which had received subcutaneous injection of 10 mg cocaine hydrochloride and 2.0 mL of 30% (v/v) ethanol 3 h
before sampling; the mean levels of cocaethylene and cocaine were 101 and 1230 pmol mL−1, respectively. 相似文献
2.
A high-performance liquid chromatography–UV method for determining DCJW concentration in rat plasma was developed. The method
described was applied to a pharmacokinetics study of intramuscular injection in rats. The plasma samples were deproteinized
with acetonitrile in a one-step extraction. The HPLC assay was carried out using a VP-ODS column and the mobile phase consisting
of acetonitrile–water (80:20, v/v) was used at a flow rate of 1.0 mL min−1 for the effective eluting DCJW. The detection of the analyte peak area was achieved by setting a UV detector at 314 nm with
no interfering plasma peak. The method was fully validated with the following validation parameters: linearity range 0.06–10 μg mL−1 (r > 0.999); absolute recoveries of DCJW were 97.44–103.46% from rat plasma; limit of quantification, 0.06 μg mL−1 and limit of detection, 0.02 μg mL−1. The method was further used to determine the concentration–time profiles of DCJW in the rat plasma following intramuscular
injection of DCJW solution at a dose of 1.2 mg kg−1. Maximum plasma concentration (C
max) and area under the plasma concentration–time curve (AUC) for DCJW were 140.20 ng mL−1 and 2405.28 ng h mL−1. 相似文献
3.
Summary A reliable and sensitive high-performance liquid chromatographic method for the determination of the recent antidepressant
citalopram and two metabolites in human plasma has been developed. Fluorescence detection at 300 nm was used, exciting at
238 nm. Separation was obtained using a reversed-phase column (C18, 250 × 3.0 mm i.d., 5 μm) and a mobile phase. 40% acetonitrile:
60% aqueous tetramethylammonium perchlorate (pH 1.9). Calibration curves were linear over a working range: 5–300 ng mL−1 for citalopram, 2.5–150.0 ng mL−1 for desmethylcitalopram and 2.5–50.0 ng mL−1 for didesmethylcitalopram. The limits of quantitation (LOQ) were 1.5 ng mL−1 for citalopram and desmethylcitalopram and 2.0 ng mL−1 for didesmethylcitalopram. Precision data, as well as accuracy, were satisfactory and no interference from different psychotropic
drugs was found. The method was therefore suitable for therapeutic drug monitoring of citalopram and its active metabolites
in plasma of depressed patients. 相似文献
4.
Cantú MD Toso DR Lacerda CA Lanças FM Carrilho E Queiroz ME 《Analytical and bioanalytical chemistry》2006,386(2):256-263
Simple, sensitive, and reproducible off-line solid-phase microextraction and liquid chromatography (SPME/LC) methods are described
for the determination of seven anticonvulsants and tricyclic antidepressants in human plasma. Factorial design and simplex
methodology were applied in the optimization of the SPME procedure for tricyclic antidepressants analyses. Important factors
in the SPME efficiency are discussed, such as the fiber coatings (both lab-made and commercial), extraction time, pH, ionic
strength, influence of plasma proteins, and desorption conditions. The development of the lab-made fiber coatings, namely,
octadecylsilane, aminosilane, and polyurethane, are further described and applied to anticonvulsants analyses. The investigated
plasmatic range for the evaluated anticonvulsants, using CW-TPR fiber, were the following: phenylethylmalonamide (3.00–40.0 μg
mL−1), phenobarbital (5.00–40.0 μg mL−1), primidone (3.00–40.0 μg mL−1), carbamazepine and carbamazepine-epoxide (2.00–24.0 μg mL−1), phenytoin (2.00–40.0 μg mL−1), and lamotrigine (0.50–12.0 μg mL−1). The antidepressants’ linear plasmatic concentration ranged from 75.0 to 500 ng mL−1 for imipramine, amitriptyline, and desipramine, and from 50.0 to 500 ng mL−1 for nortriptyline, being in all cases, the limit of quantification represented by the lowest value. The precision (interassays)
for all investigated drugs in plasma sample spiked with different concentrations of each analyte and submitted to the described
procedures were lower than 15%. The off-line SPME/LC methodologies developed allow anticonvulsants and antidepressants analyses
from therapeutic to toxic levels for therapeutic drug monitoring. 相似文献
5.
Determination of rutin and forsythin in fruit ofForsythia suspensa(Thunb.) Vahl by capillary electrophoresis-electrochemical detection 总被引:1,自引:0,他引:1
Summary Capillary electrophoresis-amperometric detection is evaluated for simultaneous determination of rutin and forsythin. The cyclic
voltammogram, hydrodynamic voltammogram, effect of pH, buffer concentration and SDS, and percent organic modifier on separation
and detection were studied. Conditions were optimized as follows: 1.2 V detection potential; separation at 12 kV; 5 s at 15
kV for sample injection time and sample injection voltage; mobile phase 20 mM boric acid buffer; pH 8.4, containing 40 mM
SDS and 10% (v/v) acetontrile. The method gave low detection limit as 0.001 mg mL−1 and 0.0005 mg mL−1 (S/N=3), wide linear range 0.005–0.5 mg mL−1 for rutin and forsythin, respectively. The relative standard deviations of peak current and migration time for 8 consecutive
injections of the standard solution containing 0.1 mg mL−1 each compound were 4.78%, 3.63% and 6.40%, 2.95% for rutin and forsythin, respectively. In addition, levels of the two compounds
in traditional Chinese herbal drugs were easily determined. 相似文献
6.
Jie Sun Yan Liang Lin Xie An Kang Yuan Xie Wei-Dong Chen Hua Lv Guang-Ji Wang 《Chromatographia》2007,66(1-2):49-54
Olprinone is a phosphodiesterase (PDE)-3 inhibitor. This paper describes a simple, selective and sensitive method for the
quantification of olprinone in rat plasma using a liquid–liquid extraction procedure followed by liquid chromatography mass
spectrometric (LC–MS) analysis. The method had an advantage of high sensitivity. Analyses were conducted at a flow rate of
0.25 mL min−1 by a gradient elution. The detection utilized selected ion monitoring in the positive ion mode at m/z 251.0 and 344.0 for the protonated molecular ions of olprinone and the internal standard, respectively. The quantitation
limit for olprinone in rat plasma was 0.5 ng mL−1. The linearity was also excellent over the concentration range of 0.5–100 ng mL−1 of olprinone. The intra- and inter-day precision (relative standard deviation (RSD) %) was lower than 10%, and accuracy ranged
from 90 to 110%. This developed method was successfully applied to analysis of olprinone in biological fluids. 相似文献
7.
A simple, sensitive and accurate spectrophotometric method for the determination of sulphonamides (sulphamethoxazole (SMZ),
sulphaguanidine (SGD), sulphaquinoxaline sodium (SQX), sulphametrole (SMR), and sulphadimidine sodium (SDD)) has been developed.
The charge-transfer reactions between sulphonamides as n-electron donors and 7,7,8,8-tetracyanoquinodimethane (TCNQ), 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), and 2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone
(chloranilic acid, p-CLA) as π-acceptors resulting in highly coloured complexes were studied. Experimental conditions for these CT reactions were carefully
optimised. Beer’s law is valid over the concentration ranges from 4–280 μg mL−1, 4–260 μg mL−1, 4–200 μg mL−1, and 4–200 μg mL−1 of SMZ, SGD, SQX, and SDD using DDQ reagent, respectively. While the calibration curves are linear in the concentration ranges
from 4–180 μg mL−1, 4–80 μg mL−1, 4–60 μg mL−1, 4–180 μg mL−1, and 4–60 μg mL−1 of SMZ, SGD, SQX, SMR, and SDD, respectively, using TCNQ reagent and from 4–380 μg mL−1 and 4–300 μg mL−1 of SQX and SDD, respectively, using p-CLA reagent, respectively. Different analytical parameters, namely molar absorptivity
(ε), standard deviation, relative standard deviation, correlation coefficient, limit of detection, and limit of quantification,
were calculated. The results obtained by the proposed methods are in good agreement with those obtained by the official method
as indicated by the percent recovery values. 相似文献
8.
Valeria F. Soares Leda R. Castilho Elba P. S. Bon Denise M. G. Freire 《Applied biochemistry and biotechnology》2005,121(1-3):311-319
A Bacillus subtilis isolate was shown to be able to produce extracellular protease in solid-state fermentations (SSF) using soy cake as culture
medium. A significant effect of inoculum concentration and physiological age on protease production was observed. Maximum
activities were obtained for inocula consisting of exponentially growing cells at inoculum concentrations in the range of
0.7–2.0 mg g−1. A comparative study on the influence of cultivation temperature and initial medium pH on protease production in SSF and
in submerged fermentation (SF) revealed that in SSF a broader pH range (5–10), but the same optimum temperature (37°C), is
obtained when compared to SF. A kinetic study showed that enzyme production is associated with bacterial growth and that enzyme
inactivation begins before biomass reaches a maximum level for both SF and SSF. Maximum protease activity and productivity
were 960 U g−1 and 15.4 U g−1 h−1 for SSF, and 12 U mL−1 and 1.3 U mL−1 h−1 for SF. When SSF protease activity was expressed by volume of enzyme extract, the enzyme level was 10-fold higher and the
enzyme productivity 45% higher than in SF. These results indicate that this bacterial strain shows a high biotechnological
potential for protease production in solid-state fermentation. 相似文献
9.
Determination of eight penicillins in serum from cattle and pigs by generic HPLC method 总被引:1,自引:0,他引:1
Summary An HPLC method was developed for determination of amoxicillin, penicillin G, penicillin V, ampicillin, oxacillin, cloxacillin,
nafcillin and dicloxacillin in serum from pigs and cattle. Serum was cleaned up by solid-phase extraction (SPE), ultra-filtered
and derivatised. The method was linear in the range tested up to 2000 ng mL−1 of individual penicillins in serum. Limits of detection were 11–14 ng mL−1. Mean recoveries were 90–103% in the range 20–2000 ng mL−1. The relative repeatability, standard deviation was <10% at 20 ng mL−1 level and <6% in the range 100–2000 ng mL−1. 相似文献
10.
M. A. Campanero A. M. Zamarreño M. Simón M. C. Dios J. R. Azanza 《Chromatographia》1997,46(7-8):374-380
Summary A simple and rapid liquid chromatographic method has been developed for the determination of therapeutic levels of piperacillin
(I) and ceftazidime (II) in human plasma. Plasma and p-propionamidophenol (internal standard) were precipitated with methanol
(I) or 20% trichloroacetic acid (II). The supernatant was analysed on a 5 μm Spherisorb ODS C18 column with acetonitrile-0.05 M phosphate buffer pH 3.8 as mobile phase and ultraviolet detection at 254 nm. The calibration
graph was linear from 10 to 250 μg mL−1, for (I), and from 5 to 200 μg mL−1 for (II). Intra and inter-day CV did no exceed 2.29% for (I), and were 10.76–11.13%–2.00–5.62 for (II) at concentrations
of 10 μg mL−1 and 250 μg mL−1. 相似文献
11.
M. A. Raggi C. Sabbioni V. Pucci N. Ghedini N. Calonghi G. Gerra 《Chromatographia》2001,53(7-8):409-413
Summary This study deals with the development of a new HPLC method for the determination of 3-methoxy-4-hydroxyphenylglycol (MHPG),
the main noradrenaline metabolite in human plasma. A Varian reversed-phase column (C8; 250 mm×4.6 mm i.d.; 5 μm particles) was used as the stationary phase and an aqueous solution of citric acid, 1-octanesulfonic
acid, EDTA, and methanol was used as the mobile phase. Coulometric electrochemical detection (ED) was used to obtain the highest
sensitivity. Isolation of MHPG from plasma was accomplished by means of a new solid-phase extraction procedure after a protein
precipitation step. The extraction yield of MHPG from plasma was very high (>97%). Linearity was observed in the 0.5–25 ng
mL−1 concentration range; the limit of detection was 0.2 ng mL−1 and the limit of quantitation was 0.5 ng mL−1. Repeatability (RSD,%) for plasma samples was found to be <3.2% and intermediate precision was <4.3%. The method was applied to the determination
of MHPG in the plasma of healthy subjects under experimentally-induced psychological stress. 相似文献
12.
Summary A method for the determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in
plasma was developed. 5-HNMP and 2-HMSI are metabolites to the widely used organic solvent N-methyl-2pyrrolidone (NMP). The
5-HNMP and 2-HMSI were purified from plasma by C8 solid phase extraction, derivatised by bistrimethylsilyl trifluoroacetamid,
and analysed by gas chromatography with mass spectrometric detection. For 5-HNMP, the precision was 2–7 % (120 and 780 ng
mL−1) and the detection limit was 6 ng mL−1 (m/z 98). For 2-HMSI, the precision was 2–9 % (160 and 1000 ng mL−1) and the detection limit was 4 ng mL−1 (m/z 144). The method is applicable for analysis of plasma samples from workers exposed to NMP. 相似文献
13.
Russo MV Notardonato I Cinelli G Avino P 《Analytical and bioanalytical chemistry》2012,402(3):1373-1381
A solid-phase extraction (SPE) method was developed for extraction and analysis of six phthalate esters in wine samples using
Carbograph 1 sorbent. The SPE procedure allowed efficient recovery of the investigated phthalates ranging between 78% and
105% with a relative standard deviation (RSD) ≤6.5 for an ethanolic phthalic acid ester (PAE) standard solution and between
73–71% and 96–99% with a RSD ≤8.4 for red wine samples spiked with 20 and 50 ng mL−1 of PAE, respectively. The adsorption isotherms and breakthrough curves for Carbograph 1/water solution were reported. Gas
chromatography coupled with an ion-trap mass spectrometer detector (GC/IT-MS) was used for analysis. The instrumental analytical
protocol was found to yield a linear calibration in the range 0.01-10.0 μg mL−1 with R
2 values ≥0.9992. The limits of detection in GC/IT-MS (SIM mode) vary between 0.2 and 14 ng mL−1 (RSD ≤5.6) whereas the limits of quantification range between 0.5 and 25 ng mL−1 (RSD ≤5.9); the intra- and inter-day repeatabilities calculated as RSD for wine samples, were between 0.9–7.8 and 1.0–10.5,
respectively. The analytical method developed was applied to several commercial wine samples. Furthermore, the investigated
methods are simple, reliable, reproducible, and not expensive. 相似文献
14.
A sensitive and selective HPLC–UV method established for determination of picroside I in dog plasma has been used to study
the pharmacokinetics of the drug after intravenous administration of three different doses. Sample pretreatment consists in
deproteination by addition of acetonitrile; l-ascorbic acid was used to improve the stability of picroside I. The lower limit of quantification of picroside I was 0.05 μg mL−1. The recovery of the method was up to 90%. After intravenous administration to dogs picroside I was mainly distributed in
the central compartment and was rapidly eliminated from the plasma; the mean elimination half-life was 30.54 ± 4.34, 30.20 ± 3.78,
and 34.02 ± 1.88 min for doses of 2.5, 5, and 15 mg kg−1, respectively, and the respective values of AUC
0–∞ were 81.04 ± 19.95, 198.50 ± 27.77, and 586.44 ± 103.08 μg min mL−1. The different doses had no significant effect on the main pharmacokinetic data and the kinetics seemed to be linear in dosage
range 2.5–15 mg kg−1. 相似文献
15.
Neng Zhou Yi-Zeng Liang Ben-Mei Chen Ping Wang Xian Chen Feng-Ping Liu 《Chromatographia》2007,66(7-8):481-486
A rapid, simple and specific liquid chromatography-electrospray ionization mass spectrometry method has been developed and
validated for the determination of hydroxyzine hydrochloride in human plasma. Samples were separated using a Thermo Hypersil-HyPURITYC18
reversed-phase column (150 mm × 2.1 mm i.d., 5 μm). The mobile phase consisted of 50 mM ammonium acetate (pH 4.0)–methanol–acetonitrile
(45:36:19, v/v). Hydroxyzine and its internal standard were measured by electrospray ion source in positive selective ion monitoring mode.
The method was validated with a linear range of 1.56–200.0 ng mL−1 and the lowest limit of quantification was 1.56 ng mL−1 for hydroxyzine hydrochloride (r
2= 0.9991). The extraction efficiencies were about 70% and recoveries of the method were in the range of 93.5–104.4%. The intra-day
relative standard deviation (RSD) was less than 8.0% and inter-day RSD was within 7.4%. QC samples were stable when kept at
ambient temperature for 12 h at −20 °C for 30 days and after four freeze–thaw cycles. The method has been successfully applied
to the evaluation of pharmacokinetics and bioequivalence of two hydroxyzine hydrochloride formulations in 12 healthy Chinese
volunteers after an oral dose of 25 mg. 相似文献
16.
Dian-Lei Wang Yan Liang Lin Xie Tong Xie X. T. Wang Sen Yu Guang-Ji Wang Xiao-Dong Liu 《Chromatographia》2008,67(3-4):219-224
To evaluate the pharmacokinetics of a novel analogue of ginkgolide B, 10-O-dimethylaminoethylginkgolide B (XQ-1) in rat plasma in pre-clinical studies, a sensitive and specific liquid chromatographic
method with electrospray ionization mass spectrometry detection (LC–ESI–MS) was developed and validated. After a simple extraction
with ethyl acetate, XQ-1 was analyzed on a Shim-pack C18 column with a mobile phase of a mixture of 1 μmol L−1 ammonium acetate containing 0.02% formic acid and methanol (55:45, v/v) at a flowrate of 0.3 mL min−1. Detection was performed in selected ion monitoring (SIM) mode using target ions at [M + H]+
m/z 496.05 for XQ-1 and m/z 432.10 for the internal standard (lafutidine). Linearity was established for the concentration range from 2 to 1,000 ng mL−1 . The extraction recoveries ranged from 86.0 to 89.9% in plasma at concentrations of 5, 50, and 500 ng mL−1. The lower limit of quantification was 2 ng mL−1 with 100 μL plasma. The validated method was successfully applied to a pharmacokinetic study after intragastic administration
of XQ-1 mesylate in rats at a dose of 20 mg kg−1. 相似文献
17.
Qing Li Liang Xu Ting-Ting Wang Ying Jia Zhi-Wei Wang Kai-Shun Bi 《Chromatographia》2008,67(7-8):627-631
Aidi injection is a clinical medicine used in China for the treatment of cancer. Calycosin-7-O-β-d-glucoside is the main effective components of the formulas. In this study, a high performance liquid chromatographic (LC)
method was developed to quantify calycosin-7-O-β-d-glucoside in rat plasma using a liquid–liquid extraction and ultraviolet (UV) absorbance detection. LC analysis was performed
on a Diamonsil C18 column (200 × 4.6 mm i.d., 5 μm particle size) with isocratic mobile phase consisting of acetonitrile–0.05% phosphoric acid
(19.5:80.5, v/v) of a flow rate of 1.0 mL min−1. The linear range was 0.11–17.6 μg mL−1 and the low quantification limit was 0.11 μg mL−1 (S/N = 10). The intra- and inter-day relative standard deviations (RSD) in the measurement of quality control (QC) samples
0.11, 0.22, 1.32 and 8.80 μg mL−1 ranged from 4.1 to 6.3 and 4.3 to 6.2%, respectively. The accuracy was from −6.7 to 4.3% in terms of relative error (RE).
Calycosin-7-O-β-d-glucoside was stable in storage at −20 °C for 2 weeks and stable after three freeze–thaw cycles in rat plasma. This method
was validated for specificity, accuracy, precision and was successfully applied to pharmacokinetic study of calycosin-7-O-β-d-glucoside in rat plasma after intravenous administration of Aidi lyophilizer. 相似文献
18.
Alaa S. Amin Ibrahim S. Ahmed Hassan A. Dessouki Hassan A. Mohamed 《Chemical Papers》2010,64(3):278-284
Simple and rapid spectrophotometric methods have been developed for the microdetermination of fluoxetine HCl. The proposed
methods are based on the formation of ion-pair complexes between fluoxetine and bromophenol blue (BPB), bromothymol blue (BTB),
bromocresol green (BCG), and bromocresol purple (BCP) which can be measured at optimum λmax. Optimization of reaction conditions was investigated. Beerșs law was obeyed in the concentration ranges of 0.5–8.0 μg mL−1, whereas optimum concentration as adopted from the Ringbom plots was 0.7–7.7 μg mL−1. The molar absorptivity, Sandell sensitivity, and detection limit were also calculated. The most optimal and sensitive method
was developed using BCG. The correlation coefficient was 0.9988 (n = 6) with a relative standard deviation of 1.25, for six determinations of 4.0 μg mL−1. The proposed methods were successfully applied to the determination of fluoxetine hydrochloride in its dosage forms and
in biological fluids (spiked plasma sample) using the standard addition technique. 相似文献
19.
Li C Wen D Zhang J Chen Z Cong W Rao Z Liu H 《Analytical and bioanalytical chemistry》2006,386(7-8):1985-1993
Metabolism of four tobacco-specific N-nitrosamines (TSNAs), N′-nitrosonornicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB), and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) has been studied by solid-phase extraction
(SPE) and liquid chromatography–tandem mass spectrometry (LC–MS–MS). 4-(Methylnitrosamino)-4-(3-pyridyl)-1-butanol (iso-NNAL)
was used as internal standard. SPE and LC–MS–MS was found to be a rapid, simple, sensitive, and selective method for analysis
of TSNAs in rabbit serum. The relative standard deviation (R.S.D., n = 6) for analysis of 5 ng mL−1 and 0.5 ng mL−1 standards and of serum sample spiked with 5 ng mL−1 standards of five TSNAs was 2.1–11% and recovery of 5 ng mL−1 standards from serum was 100.2–112.9%. A good linear relationship was obtained between peak area ratio and concentration
in the range of 0.2–100 ng mL−1 for NNAL and 0.5–100 ng mL−1 for other four TSNAs, with correlation coefficients (R
2) >0.99 (both linear and log–log regression). Detection limits for standards in solvent were between 0.04 and 0.10 ng mL−1. Doses of TSNAs administered to rabbits via the auricular vein were 4.67 μg kg−1 and 11.67 μg kg−1, in accordance with the different levels in cigarettes. Metabolic curves were obtained for the four TSNAs and for 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol
(NNAL), a metabolite of NNK; on the basis of these curves we modeled metabolic kinetic equations for these TSNAs by nonlinear
curve fitting. 相似文献
20.
Lei Zhang Liang Xu Xiao-Jie Tan Qiong-Feng Liao Wei Guo Xiao-Hui Chen Kai-Shun Bi 《Chromatographia》2007,66(1-2):115-120
A sensitive and reliable ion-paired high-performance liquid chromatographic method has been established for the simultaneous
quantification of six major active ingredients, namely baicalin, baicalein, wogonin, oxysophocarpine, oxymatrine and matrine
in the Chinese herbal preparation, Sanwu-Huangqin-Tang. HPLC analyses were performed on a Phenomenex luna C18 column with mobile phase of methanol–acetonitrile–aqueous phosphoric acid at a flow rate of 0.9 mL min−1. The complete separation was achieved within 35 min for the six target constituents. A good linear regression relationship
between peak-areas and concentrations was obtained over the range of 12.10–242.0 μg*mL−1 for baicalin, 5.05–101.0 μg*mL−1 for baicalein, 0.95–19.0 μg*mL−1 for wogonin, 2.75–55.0 μg*mL−1 for oxysophocarpin, 2.75–55.0 μg*mL−1 for oxymatrine and 4.90–98.0 μg*mL−1 for matrine, respectively. The repeatability was evaluated by intra- and inter-day assays with relative standard deviation
(RSD) being less than 5.1%. The recoveries, measured at three concentration levels, varied from 93.8 to 102.1%. The assay
was successfully applied for determination of six bioactive compounds in Sanwu-Huangqin-Tang. The interaction of chemical
constituents was observed when the herbs were used in compatibility. The results indicated that the developed assay method
was rapid, accurate and could be readily utilized as a quality control method for Sanwu-Huangqin-Tang. 相似文献