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采用盆栽试验研究了Nd3 在不同诱导时间对番茄叶抗性指标(过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性、细胞膜透性、氧自由基(O2-·)产生速率和木质素含量)的影响.结果表明:从第3 d开始,Nd3 诱导提高番茄叶内PAL活性,第3d增幅也最大,达32.30%,维持时间为诱导后第3~5 d;Nd3 诱导后第1~2 d POD活性升高,第1 d增幅最大,维持时间是诱导后第1~2 d;Nd3 诱导后1~3 d,PPO活性升高,第1 d增幅最大,维持时间是诱导后第1~3 d;从诱导后第2 d开始,Nd3 降低细胞膜透性,第3 d时降幅最大,为54.33%,持续时间为诱导后第2~5 d;从第3 d开始,Nd3 诱导降低氧自由基产生速率,第4 d降幅最大,第3 d氧自由基产生速率最低,持续时间为诱导后第3~5 d;从第4 d开始,Nd3 诱导提高木质素含量,5 d时有继续升高的趋势.从不同诱导时间番茄叶PAL活性、细胞膜透性、氧自由基产生速率的变化来看,在Nd3 诱导的第3 d,番茄产生的抗性最强. 相似文献
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《化学分析计量》2007,16(1):80
一氧化碳和甲烷一体化光纤传感测量系统主要包括第一个DFB激光源、第二个DFB激光源、第一个波形发生器、第二个波形发生器、第一个波长选择与调制器、第二个波长选择与调制器、光纤耦合器、传感探头、光电转换模块、信号调理与数据采集模块和微处理器。连接方式如下:第一个波形发生器作用于第一个波长选择与调制器,第一个波长选择与调制器控制第一个DFB激光源,第一个DFB激光源通过光纤连接耦合器的一个输入端;第二个波形发生器作用于第二个波长选择与调制器,第二个波长选择与调制器控制第二个DFB激光源,第二个DFB激光源通过光纤连接耦合器的另一个输入端;耦合器的输出端连接传感探头;传感探头连接光电转换模块; 相似文献
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1教学内容分析本课时选自鲁科版必修1第三章第3节"硫的转化"的第2课时。在之前的第1课时学生了解了含硫物质在自然界中转化以及硫单质的性质。本节课在此基础上,引导学生运用氧化还原反应理论探究在实验室实现不同价态硫元素之间的转化。通过 相似文献
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运用UPLC-MS联用技术对空白对照组、柴胡总皂苷(SS)组大鼠在给药后第3天和第5天的尿液样本进行分析检测,获得了以质荷比和保留时间为变量的矩阵数据。据此建立各组大鼠的尿液代谢轮廓图,并利用主成分分析(PCA)建立了SS的代谢组学毒性模型。结合肝组织病理,探讨了柴胡总皂苷的急性肝毒性。对于SS组与正常组,大鼠尿液代谢轮廓图表现出了一定的差异。在代谢组学毒性模型中,给药第3天和第5天的PCA分类均偏离正常组,而第5天的偏离程度强于第3天。同时,在第5天的肝组织病理切片中发现了组织病变。由此表明,给药组大鼠的代谢组偏离了正常组,这种偏离正是肝急性毒性的表现,且给药到第5天的肝毒性强于第3天,表明肝毒性与给药累积剂量呈正相关,表现出了明显的急性和累积肝毒性。 相似文献
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The stereospecific hydrolysis of mandelate can be effectively catalyzed by hyperthermophilic acylpeptide esterase APE 1547(Aeropyrum pernix esterase 1547). APE 1547 used in this reaction showed a remarkable stereodiscrimination in favour of R-mandelic acid(99% e.e.) with an enantiomeric ratio E>200. The results of computer simulation are consistent with the experimental results. It can be inferred that the R-substrate adopted a binding mode productive of the reaction due to the formation of the hydrogen bond at the active site of APE 1547. 相似文献
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GAO Ren-jun XIE Gui-qiu ZHOU Jun FENG Yan CAO Shu-gui 《高等学校化学研究》2006,22(2):168-172
Introduction Esterases(EC3.1.1.x)representadiversegroup ofhydrolasescatalyzingthecleavageandformationof esterbonds.Theyarewidelydistributedinanimals,plantsandmicroorganisms.Becauseoftheiractivities inbothaqueousandnonaqueoussolventsystems,ester aseshavebe… 相似文献
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研究了纯化的超嗜热酯酶APE1547的稳定性. 结果表明, 该酶的稳定性非常好, 蛋白的质量浓度为0.4 mg/mL时, 90 ℃的半衰期为20 h, 0.2 mg/mL时的半衰期为12 h; 而蛋白的质量浓度为0.04 mg/mL时, 保温2.5 h时残余活力仍在50%以上. 同时还研究了热变性时该酶表面疏水氨基酸的变化. 该酶的pH稳定性也很好, pH在6.5-9.0范围内作用24 h, 酶依然很稳定, 残余酶活力大于93%; 同时该酶还具有很强的耐有机溶剂的特性. 相似文献
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To improve the activity and enantioselectivity of hyperthermophilic archaeon Aeropyrum pernix K1 esterase (APE1547) and its mutants, they were purified by acetone-treated method. It was found that the acetone treatment not only caused APE1547 and its mutants to display higher activity and enantioselectivity but also saved more than 90% of time spent in purifying them by Ni-chelating column. In hydrolysis of p-nitrophenyl caprylate, the acetone-treated APE1547 and mutant A containing the following substitutions R11G, L36P, V225A, I551L, and A564T showed 5.7- and 6.9-fold active increase, respectively. In the resolution of 2-octanol acetate, the acetone-treated mutant A had a 9-fold enantioselective increase relative to that purified by Ni-chelating column. In addition, the impact of pH, temperature, and chemical reagents on activity of APE1547 and mutant A was discussed in this paper. 相似文献
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Wang Haoran Wang Zhi Zhang Hong Chen Ge Yue Hong 《Green Chemistry Letters and Reviews》2014,7(2):145-149
The asymmetric aldol addition of 2-butanone and 4-nitrobenzaldehyde catalyzed by a novel thermophilic esterase (APE1547) from the archaeon Aeropyrum pernix K1 was successfully conducted in organic solvents. APE1547 exhibited a good enzyme activity and enantioselectivity in the reaction. The effects of organic solvent, temperature, water content, and substrate concentration were investigated. The reaction provided optically active secondary alcohol with satisfying enantioselectivity (71.2 %ee) and enzyme activity (38.1 µmol/g/h) under the optimum conditions. A high yield (68.7%) could be obtained when the reaction time was approximately 120 h. 相似文献
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XIE Xiao-na ZHANG Chun-li XUN Er-na WANG Jia-xin ZHANG Hong WANG Lei WANG Zhi 《高等学校化学研究》2012,28(2):225-229
The regioselective acylation of quercetin catalyzed by a novel thermophilic esterase(APE1547)from the archaeon Aeropyrum pernix K1 was successfully conducted in organic solvents.The effects of acyl don... 相似文献
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RAO Lang BI Yun-feng XIE Gui-qiu ZHANG Fei WANG Yan CAO Shu-gui GAO Ren-jun . Key Laboratory for Molecular Enzymology Engineering Ministry of Education Jilin University Changchun P. R. China . College of Food Science Engineering Jilin Agricultural University Changchun . School of Pharmacy Changchun 《高等学校化学研究》2009,25(3)
Hyperthermophilic enzyme APE1547 is an extremely thermostable recombinant protein from thermophilic archaeon Aeropyrum pernix K1. The Tyr444 located in the catalytic domain adjacent to the catalytic amino acid Ser445 and formed hydrogen bond with Ile567. To study the effect of Tyr444 on the activity of APE1547,site-directed mutagenesis was applied. Two mutant enzymes T444S and T444G were created. Comparison of the mutant enzymes with wide enzyme,the thermostability of mutants T444S and T444G decreased by 10... 相似文献
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B3LYP/6-311+G* theoretical calculations have been employed to investigate the complexes involving hydrogen bonding between
guanine and acrylamide. Nine stable conformers were obtained by geometry optimization without imaginary frequencies. The calculation
results revealed that the stability of these complexes was accounted for by the intensity and numbers of hydrogen bonds between
guanine and acrylamide, which was proved by the energy analysis and the topological properties at the critical points. In
these optimized complexes, the complex with three hydrogen bonds was the most stable one because it offered the biggest binding
energy. Clearly, the hydrogen bonds appear to be crucial in the stability of these complexes. This work will provide another
nosogenetic interpretation besides the covalent interactions between DNA and acrylamide, which are of interest for studying
DNA mutation. 相似文献
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采用Oniom方法(B3LYP/LANL2DZ∶PM3)对脲基取代的二(9-冠-3)杯[4]芳烃衍生物(H)与离子对Na+X-(X=F-,Cl-,Br-)的包合作用进行了理论研究.在上述体系中存在2种包合方式:一是阴、阳离子在包合物中是分离的,阴离子与主体分子的上沿主要以N—H…X-氢键作用;二是阴、阳离子在包合物中存在明显的离子配对作用,阴离子主要以阳离子的配对作用存在.通过对包合物Na+X-/H的结构参数、自然键轨道(NBO)以及结合能分析可知,第1种包合方式更有利.研究表明脲基取代的二(9-冠-3)杯[4]芳烃衍生物可成为有效的离子对识别受体,研究结果进一步加深了杯芳烃类受体对离子对识别机理的认识,为实验上设计合成新型离子对受体提供了理论依据. 相似文献