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流动注射化学发光法测定利血平 总被引:9,自引:0,他引:9
研究了利血平在酸性条件下与高锰酸钾和过氧化氢产生化学发光的行为,建立了流动注射化学发光测定利血平的新方法。利血平的浓度在1.0×10^-6 ̄8.0×10^-5h/mL范围内与化学发光强度呈良好的线性关系;检出限为3×10^-7g/mL。对6×10^-6g/mL利血平进行11次平行测定,得方法的相对标准偏差为1.3%。方法用于药剂中利血平含量测定,结果与药典标准方法测得值一致。 相似文献
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基于Fe(Ⅲ)氧化硫脲生成Fe(Ⅱ)的反应和Luminol-O2-Fe(Ⅱ)化学发光反应相偶合,建立了流动注射化学发光测定硫脲的方法。方法检出限为4×10-7mol.L-1,RSD为2%(n=11),方法用于白葡萄酒中硫脲的分析测定,结果满意。 相似文献
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流动注射化学发光法测定没食子酸 总被引:4,自引:0,他引:4
基于在碱性介质中,没食子酸(Gallicacid)被过氧化氢氧化能产生化学发光,而甲醛对该体系的化学发光有较强增敏作用,据此建立了流动注射化学发光法测定没食子酸的新方法。该法简单、快速、线性范围较宽。本法测定没食子酸的线性范围为2.0×10-6~2.0×10-4g/mL,检出限达5.8×10-7g/mL,采样频率为120个/小时。对4.0×10-5g/mL没食子酸溶液连续11次测定的相对标准偏差为2.52%。该方法已成功地用于橄榄中没食子酸含量的测定。 相似文献
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A method using high-performance capillary electrophoresis with electrochemical detection (CE-ED) has been developed for content comparison of phytoestrogens in both crude and parched Flos sophorae immaturus. Genistin, genistein, rutin, kaempferol and quercetin are important active constituents in this plant. The effects of several factors, such as acidity and concentration of running buffer, separation voltage, applied potential and injection time, were investigated to find the optimum conditions. Detection limits (S/N=3) ranged from 1.1 × 10–7 to 2.8 × 10–7gmL–1 for all five analytes. This method was successfully used to analyse both crude and parched Flos sophorae immaturus after a relatively simple extraction procedure, and the assay results were satisfactory. 相似文献
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LIMin-jing YOUJing-yan YAOShuang DINGLan LIUZhong-ying ZHANGHan-qi 《高等学校化学研究》2004,20(6):703-706
Three microwave-assisted extraction(MAE) procedures were studied. The first procedure was household microwave oven dynamic extraction(HMODE). The second procedure was special microwave oven bath extraction (SMOBE). The third procedure was microwave resonant cavity dynamic extraction (MRCDE). The results obtained by the three microwave-assisted extraction procedures were compared with those obtained by using traditional Soxhlet extraction. The results indicate that the MAE not only took a shorter time, but also simplified the procedure, and made the extraction a higher yield. At the same time the results obtained by the three MAE procedures were also compared with each other. 相似文献
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In this report, carbon nanotube/polystyrene (CNT/PS) composite electrodes have been fabricated as sensitive amperometric detectors of microchip capillary electrophoresis (CE) for the determination of rutin and quercetin in Flos Sophorae Immaturus. The composite electrode was fabricated on the basis of the in situ polymerization of a mixture of CNT and styrene in the microchannel of a piece of fused silica capillary under heat. The surface morphologies of the composite in the electrodes were observed by using a scanning electron microscope. The performance of this unique system has been demonstrated by separating and detecting rutin and quercetin. The new CNT-based CE detector offered significantly lower detection potentials, yielded substantially enhanced signal-to-noise characteristics, and exhibited resistance to surface fouling and hence enhanced stability. It demonstrated long-term stability and reproducibility with a relative standard deviation of less than 5% for the peak current (n = 20) and should also find a wide range of applications in conventional CE, flowing injection analysis, and other microfluidic analysis systems. 相似文献
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Wenting Zhong Chunmiao Yang Yongze Zhang Yumeng Liu Dongsheng Yang 《Molecules (Basel, Switzerland)》2022,27(18)
Polysaccharides from Flos Sophorae Immaturus (FSI) are one of its pharmacological compounds that can perform effective activities. Aiming to extract the most effective polysaccharides against hepatocellular carcinoma (HCC), the polysaccharides were separated from FSI through ultrasonic microwave extraction, and the first comparison was carried out on the characterization of the structure and its cytotoxic properties on HCC SMMC 7721 cells of undeproteinized purified polysaccharides (PFSI-1) and papain-deproteinized polysaccharides (PFSI-2) from FSI. The findings indicated that PFSI-1 and PFSI-2 had characteristic absorption peaks of polysaccharides; PFSI-1 contained three monosaccharides and PFSI-2 contained ten; and SEM, AFM, and NMR were consistent with the verification of IR polysaccharide characteristics, suggesting probable additional latent activities. The pharmacotoxic effects of both PFSI-1 and PFSI-2 on SMMC 7721 cells (p < 0.05), attenuated the migration ability of SMMC 7721 cells (p < 0.05) and promoted apoptosis (p < 0.05), with an increase in G0/G1-phase cells and decrease in S-phase cells in the PFSI-1 as well as a decrease in G0/G1-phase cells, increase in S-phase cells, and decrease in apoptosis in the PFSI-2 (p < 0.05). The significant cytotoxic effect of PFSI-2 on SMMC 7721 cells (p < 0.05) and its protective effect on human hepatic L02 cells (HL-7702) at low concentrations (p > 0.05) could indicate its potential as a new drug for the treatment of HCC. 相似文献
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A high-performance capillary electrophoresis with electrochemical detection (CE-ED) method has been developed for the determination of phytoestrogens from the pericarps and seeds of Sophora japonica L. in this work. Genistin, genistein, rutin, kaempferol and quercetin are important bioactive constituents in these plants. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time on the CE-ED procedure were investigated. Under the optimum conditions, the five analytes could be well separated within 18 min in a 75 cm length capillary (i.d. 25 microm) at the separation voltage of 16 kV in a 50 mmol L(-1) borax running buffer (pH 9.0). A 300 microm diameter carbon disk electrode was used as the working electrode positioned carefully opposite the outlet of the capillary in a wall-jet configuration at the potential of +950 mV (vs SCE). Detection limits (S/N = 3) ranged from 1.1 x 10(-7) to 2.8 x 10(-7) g mL(-1) for all fi ve analytes. This method was successfully used to analyse dried Flos sophorae immaturus, pericarps and seeds of dried Fructus sophorae after a relatively simple extraction procedure, and the assay results were satisfactory. 相似文献
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槲皮素荧光光度测定法的研究 总被引:8,自引:2,他引:8
本详细研究了表面活性剂十二烷基磺酸钠存在下,槲皮素与锆形成三元荧光光胶束配的条件,并建立了测定槲皮素的荧光光度法。在0.9mol/L盐酸介质中,槲皮素与锆(Ⅳ),SLS形成1:4:4的黄绿色荧光配合物,激发波长432。1nm,发射波长501。3nm,线性范围为0.30×10-6-1.11×10-5mol/L,检测限为3.79×10-7mol/ L。所拟方法用于芦丁酸水解产物的测定及槐花中回收率的 相似文献
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Extraction and isolation of flavonoid glycosides from Flos Sophorae Immaturus using ultrasonic‐assisted extraction followed by high‐speed countercurrent chromatography 下载免费PDF全文
Zhisheng Xie Yongjiang Sun Shingchung Lam Mingqian Zhao Zhikun Liang Xiaoxue Yu Depo Yang Xinjun Xu 《Journal of separation science》2014,37(8):957-965
A method of ultrasonic‐assisted extraction followed by high‐speed countercurrent chromatography was established for the extraction and isolation of three flavonoid glycosides, i.e. rutin, narcissin, and nicotiflorin from Flos Sophorae Immaturus. The effects of ultrasonic‐assisted extraction factors for the main flavonoid compound (rutin) from Flos Sophorae Immaturus were optimized using Box–Behnken design combined with response surface methodology. The optimum conditions were determined as ultrasonic power 83% (600 W), solvent‐to‐material ratio 56:1, methanol concentration 82% v/v, and extraction time 60 min. Three bioactive flavonol glucosides, rutin, narcissin, and nicotiflorin were isolated from Flos Sophorae Immaturus using high‐speed countercurrent chromatography. The separation was performed with a two‐phase solvent system containing ethyl acetate/n‐butanol/methanol/water (4:0.9:0.2:5, v/v). Amounts of 87 mg of rutin, 10.8 mg of narcissin, and 1.8 mg of nicotiflorin were isolated from 302 mg of crude extract of Flos Sophorae Immaturus in a one‐step separation within 160 min with purities of 99.3, 98.0, and 95.1%, respectively, as determined by HPLC with diode array detection. Their structures were characterized by UV, MS, and NMR spectroscopy. It was demonstrated that the established method was simple, fast, and convenient, which was feasible to extract and isolate active flavonoid glycosides from Flos Sophorae Immaturus. 相似文献
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黄酮类化合物是自然界中广泛存在的一类天然产物,能使冠状动脉扩张,降低血脂,增加脑血流量,对心脏血管循环有明显的改善作用[1]. 相似文献
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微波辅助流动萃取槐花中的黄酮类成分 总被引:14,自引:1,他引:14
槐花为豆科落叶乔木槐树(Sophora japonica L.)的花蕾,槐花能降低血管的通透性,所含芸香甙(芦丁)对心脏传导系统有抑制作用,能增强收缩力及输出量,降低血压;所含槲皮素可以扩张冠状动脉,降低心肌耗氧量,并能降低血脂。 相似文献