轮状病毒肠炎患儿补锌的治疗效果

目的观察分析轮状病毒(RV)肠炎患儿血锌水平的变化以及补锌治疗的临床效果。方法选择RV肠炎患儿144例,随机分为联合组和常规组,每组72例;另外,选择80例健康儿童做为对照组。常规组患儿采用常规内科治疗,联合组在常规组的基础上联合应用葡萄糖酸锌口服液。比较联合组与常规组的临床治疗效果。结果联合组治疗有效率为95.83%,明显高于常规组的84.72%(P<0.05)。结论在常规治疗的基础上联合葡萄糖酸锌口服液补锌治疗可以明显地提高治疗效果,值得推广应用。     

轮状病毒感染性腹泻患儿的微量元素锌检测结果分析

目的：检测轮状病毒感染性腹泻患儿的全血微量元素锌的水平,探讨轮状病毒感染性腹泻患儿全血微量元素锌的变化。方法对186例轮状病毒感染性腹泻患儿和675例健康对照组儿童进行全血微量元素锌测定。结果轮状病毒感染性腹泻患儿全血微量元素锌含量明显低于健康对照组儿童,差异有统计学意义（ P＜0.05）。结论轮状病毒感染性腹泻患儿全血微量元素锌水平明显下降,需要及时补充微量元素锌。     

临床护理路径用于糖尿病性白内障患者中的护理效果分析

目的探讨分析临床护理路径用于糖尿病性白内障手术患者中的护理效果。方法选取深圳市眼科医院2013—2014年收治的60例糖尿病性白内障患者,将60例患者随机分为两组,实验组患者30例,对照组患者30例。实验组患者采用临床护理路径,对照组患者采用常规护理。调查两组患者的护理满意度,并发症发病率以及住院情况。两组进行统计分析,以P0.05表示有统计学意义。结果实验组住院时间为(4.45±0.47)d,并发症发病率为23.67%,患者护理满意度为96.67%。对照组患者住院时间为(5.97±1.14)d,并发症发病率为80%,患者护理满意度为73.33%。实验组的各项指标明显优于对照组,P0.05表示有统计学意义。结论临床护理路径用于糖尿病性白内障手术患者,能够提高患者对护理的满意度,减少患者并发症的发病率,缩短患者的住院时间,保证提高患者治疗效果和生活质量,值得在临床上大力推广应用。     

高铝引起性发育成熟前人和动物的排斥性损害和主动调节保护及自身平衡稳定反应

在铝氟联合中毒的系列研究中 ,发现性发育成熟前头发和血尿高铝的骨软化畸形病人 ,肝、肾铝显著升高的大鼠和鸡的骨铝、脑铝含量在接近或明显甚至显著低于正常对照组时 ,出现了铝骨病和铝危害的其它表现 ;AlCl3 ·6H2 O对童鸡非蓄积危害阈值范围指数 >5 (2 1 40 41 9) ,较低浓度比较高浓度饲铝组病变更为显著 ,铝在一定浓度或剂量范围与反应强度可呈非正相关关系 ;一定浓度以上 ,摄铝与高氟促成铝吸收和蓄积的数倍到 1 0数倍浓度差别的病情差异并不明显。表明人和动物在地壳高铝环境中的长期进化与适应 ,于生长发育旺盛的性成熟前形成并完善了对自然条件下进入内环境高铝的排斥和主动调节保护及自身平衡稳定反应的机制 ,以避免铝危害和减轻铝的危害程度     

157例新生儿窒息的临床分析

在广州市第六人民医院分娩的１９３６例中发生新生儿窒息的高危因素依次脐带因素，产程异常，胎位异常，胎盘功能减退，早产，胎儿宫内发育迟缓及巨大儿或出头出肩困难，羊水异常，宫缩过强等，加强孕妇孕期保健，产时监护及新生儿出生后的处理可降低新生儿窒息的发生率。     

亚临床肝性脑病患者的血清锌,铜含量的测定及临床意义

检测了３０例慢性活动性肝炎患者、３０例肝硬化患者、３０例亚临床肝性脑病患者血清中的铜、锌含量，并与正常对照组进行比较分析，探讨了微量元素铜、锌的血清含量改变及其影响因素，分析其临床意义。结果表明体内的铜锌含量与肝脏功能有密切的关系。在慢性活动性肝炎时，虽然肝脏功能有一定的损害，但由于肝脏的代偿能力强，铜，锌含量仍可保持在正常水平，而在肝硬化、亚临床肝性脑病时，铜锌明显降低，而铜锌含量的降低又导致机     

用多克隆抗体构建的夹心免疫传感器检测心肌肌钙蛋白I

用表面等离子体子共振生物传感器构建对心肌肌钙蛋白I特异性的免疫传感器检测心肌肌钙蛋白I,并建立两种检测方法:直接法的最低检测限为2.5μg/L,基于传感膜上的夹心免疫法的灵敏度为0.5μg/L,检测范围为0.5～20μg/L,批内及批间精密度分别为3.5%～4.9%,6.1%～7.4%;用夹心法及国外试剂盒对40名健康献血者和20例急性心肌梗死患者血清心肌肌钙蛋白I水平进行检测,两者符合率为95%.     

门诊100例血硒临床分析

临床观察了内科门诊100例血硒化验结果。结果表明,此100例患者血硒均存在不同程度的硒缺乏。低血硒都存在各种不同程度的疾病,如:冠心病、肝病、糖尿病、胃病、癌症、反复呼吸道感染以及各种感染、反复口腔溃疡、免疫功能低下、重金属中毒等。硒缺乏已经严重影响了本地区大多数人的身体健康。     

Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics

Chronic alcohol exposure can cause myocardial degenerative diseases, manifested as cardiac insufficiency, arrhythmia, etc. These are defined as alcoholic cardiomyopathy (ACM). Alcohol-mediated myocardial injury has previously been studied through metabolomics, and it has been proved to be involved in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway concerning unsaturated fatty acids biosynthesis and oxidative phosphorylation, which tentatively explored the mechanism of ACM induced by chronic drinking. To further study alcohol-induced myocardial injury, myocardial specimens from a previously successfully established mouse model of ACM were subjected to histological, echocardiographic, and proteomic analyses, and validated by real-time quantitative polymerase chain reaction (qPCR). Results of histopathology and echocardiography showed the hypertrophy of cardiomyocytes, the dilation of ventricles, and decreased cardiac function. Proteomic results, available via ProteomeXchange with identifier PXD032949, revealed 56 differentially expressed proteins (DEPs) were identified, which have the potential to be involved in the KEGG pathway related to fatty acid biosynthesis disorders, lipid metabolism disorders, oxidative stress, and, ultimately, in the development of dilated cardiomyopathy (DCM). The present study further elucidates the underlying effects of myocardial injury due to chronic alcohol intake, laying a foundation for further studies to clarify the potential mechanisms of ACM.     

A Pilot Metabolomic Study on Myocardial Injury Caused by Chronic Alcohol Consumption—Alcoholic Cardiomyopathy

Chronic alcohol consumption leads to myocardial injury, ventricle dilation, and cardiac dysfunction, which is defined as alcoholic cardiomyopathy (ACM). To explore the induced myocardial injury and underlying mechanism of ACM, the Liber-DeCarli liquid diet was used to establish an animal model of ACM and histopathology, echocardiography, molecular biology, and metabolomics were employed. Hematoxylin-eosin and Masson’s trichrome staining revealed disordered myocardial structure and local fibrosis in the ACM group. Echocardiography revealed thinning wall and dilation of the left ventricle and decreased cardiac function in the ACM group, with increased serum levels of brain natriuretic peptide (BNP) and expression of myocardial BNP mRNA measured through enzyme-linked immunosorbent assay and real-time quantitative polymerase chain reaction (PCR), respectively. Through metabolomic analysis of myocardium specimens, 297 differentially expressed metabolites were identified which were involved in KEGG pathways related to the biosynthesis of unsaturated fatty acids, vitamin digestion and absorption, oxidative phosphorylation, pentose phosphate, and purine and pyrimidine metabolism. The present study demonstrated chronic alcohol consumption caused disordered cardiomyocyte structure, thinning and dilation of the left ventricle, and decreased cardiac function. Metabolomic analysis of myocardium specimens and KEGG enrichment analysis further demonstrated that several differentially expressed metabolites and pathways were involved in the ACM group, which suggests potential causes of myocardial injury due to chronic alcohol exposure and provides insight for further research elucidating the underlying mechanisms of ACM.     

维生素D3、钙、锌联用治疗小儿佝偻病 36例临床分析

对36例小儿佝偻病联用维生素D3，钙，锌进行了治疗，对其临床资料的分析表明。所有病例临床症状均有改善。对治疗和预防本病具有实际价值。     

儿童低锌血症临床分析

为探讨低血锌儿童的临床表现,对长沙市中心医院儿科2010年1-11月就诊的46例低血锌儿童进行症状分类统计.结果表明,在被统计的低锌血症儿童中,表现为食欲减退,消化功能障碍者24例(52.2%),表现为生长发育迟缓者12例(26.7%),表现为免疫能力降低者6例(13%),表现为脑功能障碍者4例(8.7%).提示低血锌...     

产前宫内缺氧并新生儿心肌受损临床研究

目的研究分析产前宫内缺氧并心肌受损新生儿的血清心肌酶水平与心电图动态变化。方法选择广东省英德市妇幼保健院收治产前宫内缺氧窒息的130例患儿为实验组,其中将伴有心肌损伤患儿设为实验1组(n=75),将不伴有心肌损伤的患儿设为实验2组(n=55),同时选择同期出生的60例健康新生儿为对照组,监测分析不同时间段的心电图变化以及心肌酶水平。结果实验1组与对照组比较差异有统计意义(P0.05)。对照组未出现心电图改变,而实验1组与实验2组均出现心电图变化(ST段偏移、心动过速与心动过缓等),其中实验1组1例出现房性期前收缩,2例出现Q-T间期延长;实验2组1例出现房性期前收缩,1例出现Q-T间期延长。结论与健康新生儿比较,产前宫内缺氧并心肌受损新生儿的血清心肌酶水平与心电图异常率显著升高。     

羟基自由基引起的脱氧核糖核酸损伤研究

以鲱鱼精脱氧核糖核酸(Herring sperm DNA)为研究对象,利用紫外光(UV,200～275 nm,66.4 Lx)激发纳米TiO2发生光催化作用介导产生羟基自由基(Hydroxyl radical,.OH),探讨.OH引发DNA氧化损伤特性。采用凝胶电泳和高效液相色谱(HPLC)分析法跟踪DNA损伤历程;应用电子自旋共振(Electron spinresonance,ESR)及分光光度法跟踪损伤过程氧化物种及H2O2相对浓度的变化;运用生物标准样8-羟基脱氧鸟苷(8-Hydroxy-2’-deoxyguanosine,8-OHdG)为内标物,通过HPLC分析DNA损伤产物,研究DNA损伤机理。结果表明,较单纯UV辐照或暗光(Dark)催化条件,DNA浓度10 mg/L,TiO2浓度1.5 g/L、pH 7～8,紫外光激发纳米TiO2介导产生.OH引发DNA损伤程度最大;DNA损伤为.OH氧化历程,并伴随有深度氧化过程;DNA结构中鸟嘌呤最易氧化损伤,8-OHdG为DNA氧化损伤中间产物及鸟嘌呤氧化损伤的特异产物。     

针灸治疗多囊卵巢综合征致不孕症临床疗效观察

目的就针灸治疗多囊卵巢综合征致不孕症的临床疗效进行观察与探究。方法选择广州市天河区冼村街社区卫生服务中心自2013年4月至2015年5月期间收治的多囊卵巢综合征致不孕症患者66例,依据患者来院先后顺序平均分为参照组和观察组,每组患者33例。参照组患者予以常规治疗,观察组患者采用针灸治疗,最后比对两组患者的临床治疗效果。结果经不同方法治疗后,观察组患者各项指标的改善程度好于参照组,妊娠率和排卵率也均高于参照组,组间统计后差异显著。结论在多囊卵巢综合致不孕症患者治疗中应用针灸,其临床效果显著,具有临床应用价值。