经尿道等离子电切术治疗老年良性前列腺增生的疗效与并发症分析

目的分析经尿道等离子电切(PKRP)术治疗老年良性前列腺增生(BPH)的疗效与并发症。方法选取我院收治的100例老年BPH患者,根据入院时间顺序将其分为两组,观察组(50例)使用PKRP术治疗,对照组(50例)使用TURP术治疗,对比两组的疗效及并发症。结果与对照组比较,PKRP组的平均手术时间、持续冲洗时间、留置尿管时间、住院时间明显缩短,术后6个月QOL、IPSS、Qmax及PRV值明显改善(P0.05);两组均未见发生电切综合征(TURS)等严重的并发症。结论 PKRP治疗BPH安全有效,并发症少,值得临床推广使用。     

超声评估α1-受体阻滞剂联合5α-睾酮还原酶抑制剂治疗良性前列腺增生的效果

本文以探究α1-受体阻滞剂联合5α-睾酮还原酶抑制剂治疗良性前列腺增生患者前列腺体积、内循环及组织硬度的超声评估为主要目的。选取100例良性前列腺增生患者为研究对象,随机分为对照组(50例)和联合组(50例)。两组均进行常规治疗,对照组给予患者5α-睾酮还原酶抑制剂进行治疗,联合组给予患者5α-睾酮还原酶抑制剂与α1-受体阻滞剂联合治疗。通过彩色多普勒超声检测患者的前列腺体积(PV),根据弹性成像来评估组织硬度,并检测残余尿量(PVR)、最大自由尿流率(Qmax)、前列腺特异性抗原(PSA)。观察良性前列腺增生相关指标的变化,并统计治疗效果。治疗后,联合组IPSS评分、PV、PVR、PSA均低于对照组,QOL评分、Qmax高于对照组(P<0.05)。联合组治疗效果总有效率较高(P<0.05)。α1-受体阻滞剂、5α-睾酮还原酶抑制剂结合治疗可以改善良性前列腺增生患者前列腺体积、内循环、组织硬度,促进患者康复。     

3D腹腔镜与2D腹腔镜在肾部分切除术中的临床对比研究

目的探讨研究经3D腹腔镜对肾部分切除术的应用价值。方法收集曲靖市第一人民医院2013年10月—2015年3月泌尿外科实施肾部分切除术的患者82例作为研究对象,其中44例患者选择采用2D腹腔镜技术进行了肾部分切除,设为对照组;其余38例患者选择采用3D腹腔镜技术进行了肾部分切除,设为实验组。分析并比较两组手术时间、术中出血量、术后并发症发生率,术后住院时间。结果实验组的手术时间(90.12±11.32)min、术中出血量(70.32±15.67)m L、术后并发症发生率3.2%,术后住院时间(6.5±2.5)d;对照组手术时间(114.20±10.65)min、术中出血量(100.23±15.13)m L、术后并发症发生率20.5%,术后住院时间(7.0±2.0)d。实验组的手术时间、术中出血量、术后并发症发生率、术后住院时间均少于对照组。结论与2D腹腔镜相比,高清3D腹腔镜肾部分切除术,术野清晰,手术时间短,出血量少,术后并发症少,值得在临床推广。     

TPUS检查对子宫全切术后患者PFD发生情况的评估价值

本文旨在分析经会阴超声(TPUS)检查对子宫全切术后患者盆底功能障碍(PFD)发生情况的评估价值.选取行子宫全切术的患者157例,根据术后是否出现PFD,分为未发生PFD组103例和发生PFD组54例,均行TPUS检查.结果显示,与未发生PFD组相比,发生PFD组膀胱下移距离、直肠下移距离、阴道穹窿下移距离、膀胱尿道后...     

剖宫产术中行子宫肌瘤剔除术临床疗效分析

目的通过回顾性研究剖宫产术中行子宫肌瘤剔除术的可行性和安全性。方法选择2012年1月至2014年12月在天津市武清区中医医院行剖宫产同时剔除子宫肌瘤患者26例作为研究组,同时选取同期无子宫肌瘤仅行单纯剖宫产40例作为对照组。比较两组患者间手术时间、手术出血量、住院时间、产褥病率及术后恢复情况差异。结果研究组患者手术时间显著长于对照组,P0.05。两组患者间手术出血量、术后肛门排气时间、住院时间、产褥病率、血性恶露时间、恶露排净时间等指标均无统计学差异,P0.05。结论剖宫产术中行子宫肌瘤剔除术安全可靠,具有很好的临床适用性。     

87例结节性甲状腺肿术式改进及体会分析

目的比较传统与改良甲状腺次全切除术治疗结节性甲状腺肿的疗效,探讨改良甲状腺次全切除术临床应用价值。方法选择茂名市茂南区公馆中心卫生院2010年1月—2013年12月收治的结节性甲状腺肿患者87例,其中对照组40例患者接受传统甲状腺次全切除术治疗,观察组47例患者接受改良甲状腺次全切除术治疗。比较两组患者的手术时间、术中出血量、住院时间、VAS疼痛评分及并发症、复发情况。结果观察组患者的手术时间、术中出血量、住院时间均要优于对照组,差异有统计学意义(P0.05)。观察组患者的并发症发生率、复发率及VAS疼痛评分均低于对照组,差异有统计学意义(P0.05)。结论改良甲状腺次全切除术的临床疗效更为显著,不仅减少手术及住院时间,降低术中出血量及术后并发症发生率,而且切口更为美观,提高了患者的接受度,故值得推广应用。     

预防性抗生素用于胆囊切除术对术后感染性并发症的预防作用

目的观察预防性抗生素用于胆囊切除术对术后感染性并发症的预防作用。方法选取2013年5月—2015年9月普宁市人民医院肿瘤外科收治的106例胆囊疾病患者为研究对象,按随机数字表法分为两组各53例,对照组行腹腔镜胆囊切除术治疗,观察组另行胆囊切除术及预防性抗生素治疗,观察两组围术期指标[手术时间、手术切口、术中出血量、住院时间],同时观察两组术后感染性并发症发生情况。结果观察组手术时间、手术切口、出血量、住院时间治疗指标均较对照组小,只有住院时间两组间差异显著(P0.05),其它差异均无统计学意义(P0.05)。且观察组术后感染性并发症总发生率1.89%较对照组11.32%低,但不具有统计学意义(P0.05)。结论胆囊切除术中合理使用预防性抗生素可显著减小患者住院时间,但对患者术后感染性并发症发生无显著预防作用。由于本次样本量较少,需进一步研究。     

剖宫产术前补钙预防宫缩乏力性产后出血的效果评价

目的分析探讨产妇在进行剖宫产术前补钙以预防宫缩乏力性产后出血的临床效果,总结应用体会。方法选择深圳市盐田区盐田街道计生服务所于2014年12月—2015年12月期间收治的70例进行剖宫产的产妇作为观察对象,随机分为观察组35例与对照组35例,观察组患者于手术实施前静脉滴注葡萄糖酸钙注射液,对照组患者手术实施前不使用药物,观察两组患者的术中出血量、术后出血量、手术时间及住院时间等指标。结果观察组患者的术中出血量(178.79±23.44)m L、术后出血量(216.33±32.08)m L及术后2 h累积出血量(578.04±87.39)m L均显著少于对照组,两组间比较差异有统计学意义(P0.05);观察组患者的手术时间(34.36±5.15)min及住院时间(4.02±1.46)d均显著短于对照组,两组间比较差异有统计学意义(P0.05);观察组患者对护理质量的总体满意度为97.14%,对照组对护理质量的总体满意度为80.00%,观察组显著高于对照组,两组比较差异有统计学意义(P0.05)。结论剖宫术前补钙,可以有效预防产妇产后出血,效果确切,安全可靠,值得临床推广应用。     

宫颈环形电切术联合纳米银凝胶应用于慢性宫颈炎的临床探讨

目的分析宫颈环形电切术联合纳米银凝胶应用于慢性宫颈炎的临床疗效。方法选择2014年10月至2015年10月在上栗县长平卫生院接受治疗的122例慢性宫颈炎患者作为研究对象。将122例患者随机分为常规组和实验组。常规组使用宫颈环形电切术进行治疗;实验组使用宫颈环形电切术联合纳米银凝胶进行治疗。对比两组患者的治疗效果以及阴道感染率及出血率。结果两组患者经过不同的治疗之后实验组患者的治疗总有效率(100.0%)显著高于常规组治疗总有效率(80.3%),数据差异显著,具备统计学意义(P0.05);实验组患者的阴道感染率(1.64%)及出血率(3.28%)分别明显低于常规组的阴道感染率(13.11%)与出血率(18.03%),两组数据差异显著,具备统计学意义(P0.05)。结论宫颈环形电切术联合纳米银凝胶应用于慢性宫颈炎的临床疗效显著,纳米银凝胶能够极大程度地帮助患者减少阴道出血、阴道感染的情况发生,能够很好地弥补宫颈环形电切术的不足,能够极大程度提升患者治疗效果,是慢性宫颈炎的理想治疗方式,值得临床推广。     

腹腔镜与开放手术治疗急性阑尾炎的疗效分析

目的对比分析应用腹腔镜手术治疗及开放手术治疗应对急性阑尾炎治疗的临床疗效。方法选择2014年3月至2015年3月于辽阳市弓长岭区中心医院就诊收治并确诊为急性阑尾炎的患者共计80例,进行随机分组,包括对照组40例,主要应用开放手术应对急性阑尾炎的治疗。研究组40例,主要应用腹腔镜手术应对其的治疗。观察对比两组病患围手术期的平均手术时间、术中出血量、首次肛门排气时间及手术费用及平均住院时间,并观察记录两组患者术后并发症发生情况,均作统计学分析。结果研究组40例病患围手术期的平均手术时间、术中出血量及首次肛门排气时间较之对照组均明显要低,P0.05。对照组病患手术费用较之研究组明显要高,P0.05;研究组病患平均住院时间较之对照组明显要短,P0.05。对照组病患术后并发腹腔脓肿、肠瘘,肠黏连各两例,并发切口感染5例,总并发症发生率为27.50%。研究组病患术后仅病患切口感染1例,并发症发生率为2.50%。研究组病患并发症发生率较之对照组明显要低,且P0.05。结论腹腔镜手术针对急性阑尾炎的临床治疗其疗程疗效颇优,安全性较高,具有临床推广应用价值。     

Comparative Proteomic Analysis of Human Benign Prostatic Hyperplastic Tissue Before and After Irradiation with Radioactive Nuclide

The expressed proteins were extracted from human benign prostatic hyperplastic tissues obtained with transurethral resection of the prostate before and after their irradiation with radioactive nuclide. The proteins were separated by two-dimensional gel electrophoresis and analyzed by mass spectrometry. Four proteins were differentially expressed and were identified with a database search. Three were associated with the regulation of cell motion and one was lactate dehydrogenase B, which plays an important r...     

实时超声引导经会阴/直肠前列腺系统精准活检在前列腺癌诊断中的应用价值

为探讨实时超声引导经会阴/直肠前列腺系统精准活检在前列腺癌诊断中的应用价值,本研究回顾性分析2016年1月~2018年12月于我院接受前列腺穿刺活检的150例疑似前列腺癌患者的完整临床资料,将行实时超声引导经会阴前列腺活检的74例患者纳入A组,其中前列腺特异性抗原(PSA)灰区患者26例;将行实时超声引导经直肠前列腺系统精准活检的76例患者纳入B组,其中PSA灰区患者28例,比较各组患者一般资料、前列腺癌检出率、PSA灰区前列腺癌检出率,以及术后并发症情况。结果显示,两组年龄、PSA水平、前列腺体积比较,差异无统计学意义(P>0.05);A组穿刺针数及手术时间均高于B组(P<0.05);A组PSA灰区患者前列腺癌检出率高于B组(P<0.05);A组血便发生率低于B组(P<0.05)。实时超声引导经会阴与经直肠前列腺系统精准活检在前列腺癌诊断中的检出率相当,但针对PSA灰区患者而言,经会阴穿刺活检的检出率更高,且术后血便等并发症发生率低,安全性更高。     

人性化护理对药流术效果及术后阴道出血量的影响

目的研究并分析对药流终止妊娠患者使用人性化护理后对其术后阴道出血量的影响效果。方法收集药流终止妊娠患者共130例,根据平行、单盲、随机对照的设计原则分为对照组(65例)和观察组(65例),对照组接受常规护理,观察组结合人性化护理,将两组患者的引产成功率、不良反应发生率、术后阴道出血时间以及出血量进行观察和对比。结果观察组的引产成功率明显高于对照组,观察组的不良反应发生率显著低于对照组,观察组的术后阴道出血时间显著短于对照组,观察组术后阴道出血量显著低于对照组,P0.05。结论在药流终止妊娠患者的护理过程中,人性化护理能够显著提高药流术的效果,并对术后不良反应和阴道出血量、出血时间进行干预,值得推广应用。     

Determination of prostatic androgens in 10 mg of tissue using liquid chromatography–tandem mass spectrometry with charged derivatization

A practical liquid chromatography–tandem mass spectrometric (LC-MS-MS) method for the determination of prostatic 5-dihydrotestosterone (DHT) and testosterone (T) has been developed. The prostatic androgens were extracted with MeOH–H₂O (3: 7, v/v), purified with an Oasis HLB cartridge, derivatized with the permanently charged reagent 2-hydrazino-1-methylpyridine (HMP), and subjected to LC–MS-MS analysis using electrospray ionization (ESI) operated in the positive ion mode. The derivatization with HMP was very effective at increasing the detectability using the positive-ESI-MS. The method allowed the reproducible and accurate quantification of ng g^–1 tissue levels of prostatic androgens in 10 mg of tissue. That is, the intra- and inter-assay coefficients of variation were below 8.1 and 9.3%, respectively, and the analytical recoveries of the androgens were quantitative. The limits of quantitation for DHT and T were both 1.0 ng g^–1 tissue. The developed method was used to determine DHT and T in the prostates of patients with benign prostatic hyperplasia and prostate cancer, and satisfactory results were obtained.     

Effect of β Radiation on Bcl-2 and Bax Expressions in Benign Prostate Hyperplasia Tissues

The authors chose specimens from nine normal prostate tissues（NP group）, 15 benign prostate hyperplasia（BPH） prostates（BPH group）, and 35 BPH prostates that had been treated＇with ^90Sr/^90Y Prostatic Hyperplasia Applicator（exposure group）. The expressions of bcl-2 and bax in stroma and epithelia of prostate tissues were demonstrated by means of immunohistochemical staining, and the staining positive rate was semiquantatively determined, so as to observe the expression of bcl-2 and bax genes in the prostate tissues of normal individuals and BPH patients, before and after fl radiation, and to evaluate the influence of fl radiation on bcl-2 and bax expressions. The expressions of gene bcl-2 in the prostate epithelia of NP and BPH are significantly higher than those in the prostate stroma（P〈0.01）. However, the expressions of bcl-2 in the prostate epithelia and stroma of the BPH group are obviously higher than those in the NP group（P〈0.01）. The expression of gene bax in the prostate epithelia of the NP group is higher than that in the BPH group（P〈0.05）. However, bcl-2 expressions in the prostate epithelia and stroma of the BPH group are significantly higher than the bax expressions（P〈0.01）. Compared with those of the NP group, the expressions of bcl-2 in the prostate epithelia and stroma of the exposure group decrease remarkably, even as the expressions of the bax notably increase（P〈0.01）. Thus, the administration of β radiation can remarkably affect bcl-2 and bax gene expressions, to regulate cell apoptosis, in the prostate tissues of BPH.     

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

This work aimed to immobilize the antibody anti-galectin-3 onto polysiloxane–polyvinyl alcohol (POS-PVA) support, to evaluate its capacity to capture the serum antigen galectin-3 and to quantify by ELISA the antigen levels in sera from patients with prostatic adenocarcinoma (PA) and benign prostatic hyperplasia (BPH) and healthy individuals. Also, for comparative effect, the galectin-3 expression in the prostate tissue through immunohistochemistry was evaluated. The optical density (galectin-3 level) values established for the sera from PA and BPH patients were lower compared with those found for the healthy individuals. Galectin-3 immunohistochemically showed a significant increase and reduction of the cytoplasmatic protein expression in BPH and PA, respectively, compared with the normal prostate. These results showed that POS-PVA disks could be used as solid phase to immobilize serum galectins and in immunoassays procedures for the correspondent IgG anti-galectins detection in human sera.     

Zinc-mediated intramolecular acyl and imino transfer reactions of aryl iodides

A method for the coupling of acyl and imino substituents to the sterically encumbered 5-position of a 4-aminoquinazoline was developed. Starting with a 4-amino-5-iodoquinazoline, the method employs a facile intramolecular zinc-mediated transfer from the 4-amino group to the iodo-bearing carbon. The method was found to be effective for a variety of substituents, in particular a pyridyl group required for the synthesis of Pfizer’s prostate selective α₁ antagonist candidate for the treatment of benign prostatic hyperplasia, UK-338,003.     

Protein complexes/aggregates as potential cancer biomarkers revealed by a nanoparticle aggregation immunoassay

Protein–protein interactions and protein complex/aggregate formation play an essential role in almost all biological functions and activities. Through a nanoparticle aggregation immunoassay, we discovered that some proteins are substantially more complexed/aggregated in cancer tissues than normal tissues. This study examined four biomarkers proteins, CA125, CEA (carcinoembryonic antigen), CA19-9 and PAP (prostatic acid phosphatase) in ovarian, colon and prostate tissue lysates. The most exciting results were observed from the PAP assay of prostate tissues: prostate cancer can be clearly distinguished from normal prostate and prostate with benign conditions such as BPH (benign prostate hyperplasia) based on the complex/aggregation level of PAP in prostate tissue lysates. The complex/aggregate level of a protein can be potential biomarkers for cancer detection and diagnosis.