共查询到20条相似文献,搜索用时 46 毫秒
1.
Carol L. K. Sabourin Abbie G. Freeman Donna F. Kusewitt Ronald D. Ley 《Photochemistry and photobiology》1992,55(3):417-424
Chronic exposure of the gray, short-tailed oppossum, Monodelphis domestica to ultraviolet radiation (UVR) induces mesenchymal tumors of the cornea. High molecular weight DNA samples from 6 UVR-induced corneal tumors were assayed for their ability to transform NIH 3T3 cells to tumorigenicity. NIH 3T3 cells transfected with DNA from 5 of the corneal tumors produced 14 tumors in nude mice. Cell lines were established from these tumors. DNA from 13 of 14 tumor cell lines contained repetitive opossum DNA sequences. Southern blot analysis revealed that DNA from 3 of 4 cell lines derived from tumorigenic NIH 3T3 cells transfected with DNA from a single oppossum tumor contained opossum Ki-ras oncogene sequences in addition to the murine Ki-ras gene. Northern blot analysis of mRNA from a mouse tumor cell line containing opossum Ki-ras gene sequences showed mRNA species identical in size to opossum Ki-ras mRNA, as well as murine Ki-ras mRNA species. These results suggest that an activated Ki-ras oncogene was present in one of the original opossum corneal tumors tested. Thus, activation of Ki-ras may play a role in the development of UVR-induced corneal tumors in Monodelphis domestica. Further characterization of ras oncogenes in these opossum tumors may provide information on the molecular mechanisms by which UVR induces corneal tumors in this species. 相似文献
2.
B. D. Hirsch N. C. Walz B. E. Meeker M. R. Arnfield J. Tulip M. S. McPhee J. D. Chapman 《Photochemistry and photobiology》1987,46(5):847-852
Abstract The administration of misonidazole (MISO) to Fischer x Copenhagen rats whose R3327-H prostate tumors were treated with photodynamic therapy (PDT) produced enhanced tumor growth delays and cures. This potentiation of PDT by MISO was previously observed with R3327-AT tumors and was postulated to result from drug cytotoxicity of naturally-occurring and PDT-induced hypoxic cells. Radioactively-labelled MISO has been developed as a marker for tissue p02 at the cellular level and [3 H]MISO was administered to R3327-AT and R3327-H tumor-bearing rats before and after standard PDT treatments. The amount of 3 H in tissues 24 h after drug administration was a measure of'bound MISO'which reflects average tissue oxygenation. [3 H]MISO retained in R3327-AT tumors was ˜4x and in liver tissue ˜2x that retained in muscle, heart, brain and R3327-H tumors (1x). Tumors treated with Photofrin II and lased with 1000 J showed a 6-fold increase in retained [3 H]MISO in R3327-H tumors and a 2-fold increase in retained [3 H]MISO in R3327-AT tumors. The absolute levels of retained 3 H in both tumors after PDT were similar. These data provide direct evidence that PDT induces rapid hypoxia in both tumors. When the gastrocnemius muscle of the rat leg was similarly treated, the amount of [3 H]MISO retained was ˜4x greater than that in untreated muscle. This result suggests that PDT-induced hypoxia is not selective to just tumor tissue. These data suggest that the hypoxia-inducing property of PDT might be exploited in combination with hypoxic cell cytotoxins to produce improved tumor responses and cures. 相似文献
3.
OPTICAL PROPERTIES OF EXPERIMENTAL PROSTATE TUMORS in vivo 总被引:2,自引:0,他引:2
M. R. Arnfield J. D. Chapman J. Tulip M. C. Fenning M. S. McPhee 《Photochemistry and photobiology》1993,57(2):306-311
The optical properties of tumor tissue provide important information for optimizing treatment plans in photodynamic therapy, especially when intertitial application by multiple fibers is planned. Near infrared light, required to activate novel photosensitizers, should facilitate improved light penetrance of tumor tissue compared with 630 nm light used for activating Photofrin II. We have measured light energy fluence rates for 630 and 789 nm light along radial tracks from a single laterally diffusing optical fiber centrally implanted into Dunning R3327-AT and R3327-H rat tracks from a single laterally diffusing optical fiber centrally implanted into Dunning R3327-AT and R3327-H rat prostate tumors in anesthetized rats. A total of 20 R3327-AT and 10 R3327-H tumors were used in this study with volumes from 2.6 to 13.3 cm3. Light track data were analyzed by an empirical model that described light attenuation. At 630 nm, light attenuation coefficients (LAC) were T1.9 × higher than those at 789 nm for both tumors with the well-differentiated, well-perfused tumor (R3327-H) attenuating to a greater extent than did the rapidly growing anaplastic tumor (R3327-AT). The intertumor variation of LAC was greater than the spatial variations observed within individual tumors. LAC were a function of tumor volume for only 630 nm light in the R3327-AT tumors. 相似文献
4.
E. Lukevics D. Jansone L. Leite J. Popelis G. Andreeva I. Shestakova I. Domracheva V. Bridane I. Kanepe 《Chemistry of Heterocyclic Compounds》2009,45(10):1226-1234
A series of phenylvinyl derivatives of 4,6,6-trimethyl-2-oxo-1,2,5,6-tetrahydropyridine-3-carbonitriles has been synthesized
and their cytotoxic activity towards HT-1080 (human fibrosarcoma) and MG 22A (mouse hepatoma) tumor cells studied. It was
found that the 2-nitro-, 2- and 3-chloro, 2-fluoro, and 2-bromophenyl derivatives showed high cytotoxic activity towards both
cell lines. The toxicity towards NIH 3T3 normal mouse embryonic fibroblasts depends on the nature and position of the substituent
in the phenyl ring. The greatest selectivity of cytotoxic effect was seen in the 2-bromophenyl derivative. 相似文献
5.
Kim SH Kim MO Lee SR Kim KS Lee TH Lee HT Ha JH Kim TY Ryoo ZY 《Experimental & molecular medicine》2006,38(3):196-202
We previously reported that transgenic mice produced with a transgene consisting of the SV40 T antigen and vasopressin without the 3'-flanking region exhibit brain tumors and lymphoma. In this study, transgenic mice were produced with the fusion gene containing the SV40 T antigen and the whole vasopressin gene with the 3'-flanking region. Six transgenic mice were generated, five which died after 2-6 weeks. The remaining founder mouse was investigated for fusion gene expression and tumor progression at the age of 6 weeks. Brain tumor cells were characterized for phenotypes and transgene expression. During in vitro cell cultures, the phenotypic appearances at 10, 20, and 30 passages were as a uniform monolayer with similar growth rates. The site of SV40 T antigen integration was in the A2 region of chromosome 11, and SV40 T antigen was expressed at the same level in cells of both earlier and later passages. Thirty passages were probably insufficient to reach crisis and immortalization. These cells enriched brain tumor cell compositions with astrocytes and neuronal cells. 相似文献
6.
Findlater M Schultz KM Bernskoetter WH Cartwright-Sykes A Heinekey DM Brookhart M 《Inorganic chemistry》2012,51(8):4672-4678
A series of iridium and rhodium pincer complexes have been synthesized and characterized: [(POCOP)Ir(H)(H(2))] [BAr(f)(4)] (1-H(3)), (POCOP)Rh(H(2)) (2-H(2)), [(PONOP)Ir(C(2)H(4))] [BAr(f)(4)] (3-C(2)H(4)), [(PONOP)Ir(H)(2))] [BAr(f)(4)] (3-H(2)), [(PONOP)Rh(C(2)H(4))] [BAr(f)(4)] (4-C(2)H(4)) and [(PONOP)Rh(H(2))] [BAr(f)(4)] (4-H(2)) (POCOP = κ(3)-C(6)H(3)-2,6-[OP(tBu)(2)](2); PONOP = 2,6-(tBu(2)PO)(2)C(5)H(3)N; BAr(f)(4) = tetrakis(3,5-trifluoromethylphenyl)borate). The nature of the dihydrogen-metal interaction was probed using NMR spectroscopic studies. Complexes 1-H(3), 2-H(2), and 4-H(2) retain the H-H bond and are classified as η(2)-dihydrogen adducts. In contrast, complex 3-H(2) is best described as a classical dihydride system. The presence of bound dihydrogen was determined using both T(1) and (1)J(HD) coupling values: T(1) = 14 ms, (1)J(HD) = 33 Hz for the dihydrogen ligand in 1-H(3), T(1)(min) = 23 ms, (1)J(HD) = 32 Hz for 2-H(2), T(1)(min) = 873 ms for 3-H(2), T(1)(min) = 33 ms, (1)J(HD) = 30.1 Hz for 4-H(2). 相似文献
7.
Dr. Yu Cao Dr. David T. Rodgers Dr. Juanjuan Du Insha Ahmad Eric N. Hampton Dr. Jennifer S. Y. Ma Dr. Magdalena Mazagova Dr. Sei‐hyun Choi Dr. Hwa Young Yun Dr. Han Xiao Dr. Pengyu Yang Xiaozhou Luo Dr. Reyna K. V. Lim Holly M. Pugh Dr. Feng Wang Dr. Stephanie A. Kazane Dr. Timothy M. Wright Dr. Chan Hyuk Kim Prof. Peter G. Schultz Dr. Travis S. Young 《Angewandte Chemie (International ed. in English)》2016,55(26):7520-7524
Chimeric antigen receptor T (CAR‐T) cells have demonstrated promising results against hematological malignancies, but have encountered significant challenges in translation to solid tumors. To overcome these hurdles, we have developed a switchable CAR‐T cell platform in which the activity of the engineered cell is controlled by dosage of an antibody‐based switch. Herein, we apply this approach to Her2‐expressing breast cancers by engineering switch molecules through site‐specific incorporation of FITC or grafting of a peptide neo‐epitope (PNE) into the anti‐Her2 antibody trastuzumab (clone 4D5). We demonstrate that both switch formats can be readily optimized to redirect CAR‐T cells (specific for the corresponding FITC or PNE) to Her2‐expressing tumor cells, and afford dose‐titratable activation of CAR‐T cells ex vivo and complete clearance of the tumor in rodent xenograft models. This strategy may facilitate the application of immunotherapy to solid tumors by affording comparable efficacy with improved safety owing to switch‐based control of the CAR‐T response. 相似文献
8.
E. Lukevics I. Shestakova I. Domracheva A. Nesterova D. Zaruma J. Ashaks 《Chemistry of Heterocyclic Compounds》2006,42(6):761-764
High cytotoxicity has been established for the 8-quinolinethiolates of copper, cadmium, indium, antimony, bismuth, ruthenium,
rhodium, palladium, osmium, iridium, and platinum on HT-1080 (human fibrosarcoma), MG-22A (mouse hepatoma), and B-16 (mouse
melanoma) tumor cells. The greatest activity against HT-1080 was possessed by the iridium complex, and against MG-22A by the
osmium complex. All the investigated metal 8-quinolinethiolates were highly toxic in relation to NIH 3T3 normal mouse embryo
fibroblasts.
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Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 6, pp. 870–873, June, 2006. 相似文献
9.
在缺失了3'LTR U3区内病毒的启动子/增强子序列的逆转录病毒载体pLXSNd中,用血管内皮生长因子受体KDR的特异性启动子调控了TNFa在血管内细胞ECV304中的靶向表达。将构建的载体pLXSN-TNFa,pLXSNd-KDRp-TNFa和空载体pLXSN用PA317细胞包装后获得重组病毒,并用重组病毒分别感染NIH3T3细胞和ECV304细胞,培养物上清的ELISA结果证明,KDR启动子指导的TNFa在KDR阳性细胞ECV304中的表达量为在KDR阴性细胞NIH3T3中的表达量的8倍;而TR指导的TNFa在这两种细胞中的表达无明显差异,实现了TNFa在血管内皮细胞中的靶向表达,这可能为肿瘤基因治疗提供新途径。 相似文献
10.
Revzin A Rajagopalan P Tilles AW Berthiaume F Yarmush ML Toner M 《Langmuir : the ACS journal of surfaces and colloids》2004,20(8):2999-3005
In this study, robotic protein printing was employed as a method for designing a cellular microenvironment. Protein printing proved to be an effective strategy for creating micropatterned co-cultures of primary rat hepatocytes and 3T3 fibroblasts. Collagen spots (ca. 170 microm in diameter) were printed onto amino-silane- and glutaraldehyde-modified glass slides. Groups of 15-20 hepatocytes attached to collagen regions in a highly selective manner forming cell clusters corresponding in size to the printed collagen domains. Fibroblasts, seeded onto the same surface, adhered and spread around arrays of hepatocyte islands creating a heterotypic environment. The co-cultured hepatocytes produced and maintained high levels of liver-specific biomarkers, albumin and urea, over the course of 2 weeks. In addition, protein printing was combined with poly(ethylene glycol) photolithography to define intercellular contacts within the clusters of hepatocytes residing on individual collagen islands. Glass slides, treated with 3-acryloxypropyl trichlorosilane and imprinted with 170 m diameter collagen spots, were micropatterned with a high-density array of 30 microm x 30 microm poly(ethylene glycol) (PEG) wells. As a result, discrete groups of ca. 9 PEG microwells became functionalized with the cell-adhesive ligand. When exposed to micropatterned surfaces, hepatocytes interacted exclusively with collagen-modified regions, attaching and becoming confined at a single-cell level within the hydrogel wells. Micropatterning strategies proposed here will lead to greater insights into hepatocellular behavior and will benefit the fields of hepatic tissue engineering and liver biology. 相似文献
11.
12.
Expression of foreign genes transferred into mammalian cells by electroporation has been studied. The pX1TK gene, pSV2Neo gene and pUCEJ oncogene have been introduced into MLTK-cells and NIH/3T3 cells, respectively. Stable transformation transient expression of TK gene by MLTK-cells as well as stable and malignant transformation of NIH/3T3 cells have been obtained. Transient expression frequency is about 80% and stable transformation frequency is about 10~(-4). Integration of foreign genes into the cellular genome was verified with molecular hybridization. Tumor development was observed after inoculation of transformed celts into nude mice. 相似文献
13.
E. Lukevics D. Zaruma J. Ashaks I. Shestakova I. Domracheva A. Gulbe V. Bridane 《Chemistry of Heterocyclic Compounds》2008,44(5):559-564
It has been found that the nature of the substituent, its position in the quinoline ring, and the nature of the metal significantly
affect the antitumor activity and toxicity of metal 8-quinolinethiolates. The most cytotoxic towards human fibrosarcoma HT-1080
and mouse hepatoma MG-22A tumor cells are the 6-methoxy-8-quinolinethiolates of rhodium, osmium, iridium, indium, antimony,
and bismuth, however these are highly toxic towards normal mouse embryonic NIH 3T3 fibroblasts. The iridium 5-methyl-8-quinolinethiolate
is somewhat less active to MG-22A cells but shows quite good selectivity of action because of its markedly lower toxicity.
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Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 5, pp. 711–717, May, 2008. 相似文献
14.
J T Yang X Q Wang M Wu 《Science in China. Series B, Chemistry, life sciences & earth sciences》1989,32(5):543-550
Expression of foreign genes transferred into mammalian cells by electroporation has been studied. The pX1TK gene, pSV2Neo gene and pUCEJ oncogene have been introduced into MLTK- cells and NIH/3T3 cells, respectively. Stable transformation transient expression of TK gene by MLTK- cells as well as stable and malignant transformation of NIH/3T3 cells have been obtained. Transient expression frequency is about 80% and stable transformation frequency is about 10(-4). Integration of foreign genes into the cellular genome was verified with molecular hybridization. Tumor development was observed after inoculation of transformed cells into nude mice. 相似文献
15.
ZHANG Shu-zi ZHANG Hai-hong ZHANG Wa SHI He-liang YU Xiang-hui KONG Wei LI Wei 《高等学校化学研究》2008,24(4):505-510
Two eukaryotic vectors expressing 9 tandem repeats of human MUCI(VNTR), VR1012-VNTR, and pEGFP-VNTR, were constructed by cloning VNTR gene into VR1012 and pEGFP, respectively. VNTR stably expressing murine Lewis lung carcinoma(LLC) cell line(VNTR^+ LLC) was established by Lipofectamine-mediated transfection of pEGFP-VNTR into LLC cells. The EGFP expression was observed under a fluorescent microscope and VNTR expression in VNTR^+ LLC cells was confirmed by means of Western blotting. A syngenic graft tumor model was generated by subcutaneous injection of VNTR^+ LLC cells into C57/BL6 mice and tumor size increased rapidly with time and in a cell qumber dependent manner. VNTR mRNA expression in the tumor formed was confirmed by RT-PCR. After the third immunization mice were challenged subcutaneously with 5×10^5 VNTR^+ LLC cells, a significant reduction of subcutaneous tumor growth was observed in the groups immunized with VNTR plasmid DNA compared with that in the groups immunized with the vector DNA alone. Thus, the suppression of subcutaneous tumor was antigen-specific. This model is useful for the development of tumor vaccines targeting MUCI VNTRs. 相似文献
16.
Block Copolymer Brushes for Completely Decoupled Control of Determinants of Cell–Surface Interactions
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Inga Lilge Prof. Dr. Holger Schönherr 《Angewandte Chemie (International ed. in English)》2016,55(42):13114-13117
The known determinants for cell–surface interactions, comprising biochemical cues, patterns, passivating functionality, and control of tether mechanical properties, are fully decoupled in tailored block copolymer brushes synthesized by surface‐initiated atom transfer radical polymerization. Exploiting sequential polymerization of a passivating underlying polyacrylamide (PAAm) block with defined cross‐linking followed by a second poly(acrylic acid) block, which can be conjugated with a selective adhesion peptide, hierarchically structured brushes that can be micro‐patterned by soft lithography were obtained. The interaction of NIH 3T3 fibroblasts and PaTu 8988t pancreatic tumor cells with brushes that differed only in the stiffness of the hidden PAAm block or only in the peptide ligand, while keeping all other parameters constant, revealing profound differences in cell adhesion and morphology. In particular, cells could only attach well to stiff RGD presenting brushes. 相似文献
17.
There has been considerable interest in the use of botanical supplements to protect skin from the adverse effects of solar UV radiation, including photocarcinogenesis. We and others have shown that topical application of (-)-epigallocatechin-3-gallate (EGCG) from green tea prevents photocarcinogenesis in mice; however, the chemopreventive mechanism of EGCG in an in vivo tumor model is not clearly understood. In this study, UV-B-induced skin tumors with and without treatment of EGCG ( approximately 1 mg/cm(2)) and age-matched skin biopsies from SKH-1 hairless mice were used to identify potential molecular targets of skin cancer prevention by EGCG. These biopsies were analyzed for various biomarkers of angiogenesis and antitumor immune response using immunostaining, Western blotting and gelatinolytic zymography. We report that compared to non-EGCG-treated tumors, topical application of EGCG in UV-induced tumors resulted in inhibition of protein expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9, which play crucial roles in tumor growth and metastasis. In contrast, tissue inhibitor of MMP-1 (TIMP-1), which inhibits MMP activity, was increased in tumors. With respect to the tumor vasculature, EGCG decreased the expression of CD31, a cell surface marker of vascular endothelial cells, and inhibited the expression of vascular endothelial growth factor in tumors, which are essential for angiogenesis. EGCG inhibited proliferating cell nuclear antigen in UV-B-induced tumors as well. Additionally, higher numbers of cytotoxic T lymphocytes (CD8(+) T cells) were detected in EGCG-treated tumors compared with non-EGCG-treated tumors. Together, these in vivo tumor data suggested that inhibition of photocarcinogenesis in mice by EGCG is associated with inhibition of angiogenic factors and induction of antitumor immune reactivity. 相似文献
18.
E. Lukevics I. Shestakova I. Domracheva A. Nesterova J. Ashaks D. Zaruma 《Chemistry of Heterocyclic Compounds》2006,42(1):53-59
A series of 2-methyl-, 4-methyl-, and 2,4-dimethyl-8-quinolineselenolates of zinc, cadmium, mercury, nickel, palladium, platinum,
arsenic, antimony, and bismuth has been synthesized and their cytotoxicity has been studied on HT-1080 (human fibrosarcoma),
MG-22A (mouse hepatoma), B16 (mouse melanoma), and Neuro 2A (mouse neuroblastoma) tumor cells. Mercury complexes were distinguished
by high cytotoxicity on all the cell lines. Palladium complexes possessed somewhat lower activity and were significantly less
toxic in relation to normal NIH 3T3 mouse embryo fibroblasts. All the studied metal 2-methyl-8-quinolineselenolates displayed
high cytotoxicity on B16 melanoma, arsenic 4-methyl-8-quinolineselenolate acted most effectively on HT-1080 and MG-22A cells.
Di(4-methyl-8-quinolyl) diselenide also possessed high cytotoxicity on these same cells.
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Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 1, pp. 59–66, January, 2006. 相似文献
19.
In this study the antioxidant and cytotoxicity activity of the Adonidia merrillii fruits were investigated using different solvent polarities (methanol, ethyl acetate and water). The results showed that the total phenolic and flavonoid contents of the methanolic extract was higher compare with other extract with respective values of 17.80 ± 0.45 mg gallic acid equivalents/g dry weight (DW) and 5.43 ± 0.33 mg rutin equivalents/g DW. Beside that The RP-HPLC analyses indicated the presence of gallic acid, pyrogallol, caffeic acid, vanillic acid, syringic acid, naringin and rutin. In the DPPH, NO2 and ABTS scavenging assays, the methanolic extract exhibited higher antioxidant activity as compared to the ethyl acetate and water extracts. The extracts exhibited moderate to weak cytotoxic activity in the assays using human hepatocytes (Chang liver cells) and NIH/3T3 (fibroblasts cell) cell lines. The findings showed the Adonidia merrillii fruit extracts to possess considerable antioxidant and cytotoxicity properties. The fruit, therefore, is a potential candidate for further work to discover antioxidant and cytotoxic drugs from natural sources. 相似文献
20.
Patterned poly(acrylic acid) (PAA)/poly(allylamine hydrochloride) (PAH) multilayer films with line structures of different lateral size and vertical height were fabricated by a room-temperature imprinting technique, and their cell adhesion properties were investigated. The nonimprinted PAA/PAH multilayer films are cytophilic toward NIH/3T3 fibroblasts and HeLa cells whether PAA or PAH is the outer most layer. In contrast, the PAA/PAH multilayer films with a 6.5-microm-line/3.5-microm-space pattern structure are cytophobic toward NIH/3T3 fibroblasts and HeLa cells when the height of the lines is 1.29 microm. By either increasing the lateral size of the patters to 69-microm-line/43-mum-space or decreasing the height of the imprinted lines to approximately 107 nm, imprinted PAA/PAH multilayer films become cytophilic. This kind of transition of cell adhesion behavior derives from the change of the physical pattern size of the PAA/PAH multilayer films and is independent of the chemical composition of the films. The easy patterning of layer-by-layer assembled polymeric multilayer films with the room-temperature imprinting technique provides a facile way to tailor the cellular behavior of the layered polymeric films by simply changing the pattern dimensions. 相似文献