共查询到20条相似文献,搜索用时 15 毫秒
1.
《Radiation Physics and Chemistry》2004,69(5):395-400
The effect of electron beam irradiation on the microstructure of cellulose has been investigated using positron annihilation lifetime spectroscopy (PALS) and electron paramagnetic resonance (EPR) Spectroscopy. PALS studies of irradiated cellulose samples showed that ortho-positronium (o-Ps) lifetime increases with an increase in dose up to 80 kGy and decreases at higher doses. The EPR signal of the irradiated cellulose matrix showed the presence of multiple radical sites. These results are discussed on the basis of chemical and physical changes occurring at the microscopic level in the cellulose due to irradiation. 相似文献
2.
A. U. Ahmed W. H. Rapson 《Journal of polymer science. Part A, Polymer chemistry》1972,10(7):1945-1952
EPR studies were carried out on different irradiated cellulosic materials. The shape of the EPR spectrum was found to depend upon the fine structure of cellulose. The peak-to-peak distances of the large peaks on the differential curves were found to be 23.3 and 24.8 gauss, respectively, for the amorphous and recrystallized cellulose. The number of free radicals present per unit weight in the recrystallized cellulose and in the amorphous cellulose were found to be in the ratio of 2.1:1. The EPR spectrum of a sample of irradiated cotton crystallites which was soaked in acetone before being dried was markedly symmetrical, in contrast to the EPR spectra of samples of cotton linters and of cotton crystallites dried from water, and was considered to be a reflection of the lower intermolecular bonding in the former. For corresponding doses, the free radicals on cotton crystallites have the highest g value, followed by cotton linters, followed by regenerated cellulose I. The g value decreased as the irradiation dose was increased. 相似文献
3.
Yu Bai WenYue Jiang He Lin Zhiqiang Liu Zhongying Liu 《Journal of Radioanalytical and Nuclear Chemistry》2014,301(1):125-131
The feasibility of thermoluminescence (TL) to differentiate irradiated Chinese medicinal herbs from non-irradiated was investigated. Thirty different dried Chinese herbs were tested, including root, flower, ramulus, rhizome, cortex, and whole plant samples. Irradiation of Chinese herbs was associated with strong TL peaks at ~150–250 °C, while TL curves of non-irradiated herbs had very low intensities above 250 °C, which was also confirmed by the TL ratio (non-irradiated, TL1/TL2 < 0.1). The ability to determine the irradiation dose by the TL method was influenced by the amount and types of minerals in the samples. All levels of irradiation doses could be detected when between 0.1 and 1.0 kGy, except for three herbs at 0.1 kGy dose. Different blends with small quantities (0.1–10 %) of irradiated herbs were also tested in this study. Samples with powder mixtures containing 1 % irradiated components could be differentiated (TL1/TL2 > 0.1) except for sterculia lychnophora, semen cassia, flos inulae, and anemone root. TL ratios of some herbs indicated irradiation (TL1/TL2 > 0.1) even if the irradiated components were as low as 0.1 %. Thus we demonstrated that TL analysis had excellent sensitivity and reliability for the identification of irradiated Chinese herbs. 相似文献
4.
Sukdeb Pal Byeong Keun Kim Won Young Kim Min Jung Kim Hyeon A. Ki Kyeong-Hee Lee Woo Suk Kang In Ho Kang Shin Jung Kang Joon Myong Song 《Analytical and bioanalytical chemistry》2009,394(7):1931-1945
Dried herbal samples consisting of root, rhizome, cortex, fruit, peel, flower, spike, ramulus, folium, and whole plant of
20 different medicinal herbs were investigated using pulsed photostimulated luminescence (PPSL), thermoluminescence (TL),
and electron spin resonance spectroscopy (ESR) to identify γ-ray irradiation treatment. Samples were irradiated at 0–50 kGy
using a 60Co irradiator. PPSL measurement was applied as a rapid screening method. Control samples of 19 different herbs had
photon counts less than the lower threshold value (700 counts 60 s−1). The photon counts of non-irradiated clematidis radix
and irradiated evodia and gardenia fruits were between the lower and upper threshold values (700–5,000 counts 60 s−1). TL
ratios, i.e., integrated areas of the first glow (TL1)/the second glow (TL2), were found to be less than 0.1 in all non-irradiated
samples and higher than 0.1 in irradiated ones providing definite proof of radiation treatment. ESR spectroscopy was applied
as an alternative rapid method. In most of the irradiated samples, mainly radiation-induced cellulosic, sugar, and relatively
complicated carbohydrate radical ESR signals were detected. No radiation-specific ESR signal, except one intense singlet,
was observed for irradiated scrophularia and scutellaria root and artemisiae argyi folium.
Figure PPSL can be used as a rapid simple preliminary screening method and a combination of ESR and TL tests for a definite proof
of gamma irradiation treatment of medicinal herbs. 相似文献
5.
EPR as an analytical tool in assessing the mineral nutrients and irradiated food products-vegetables
Prasuna CP Chakradhar RP Rao JL Gopal NO 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2008,71(3):809-813
EPR spectral investigations of some commonly available vegetables in south India, which are of global importance like Daucus carota (carrot), Cyamopsis tetragonoloba (cluster beans), Coccinia indica (little gourd) and Beta vulgaris (beet root) have been carried out. In all the vegetable samples a free radical corresponding to cellulose radical is observed. Almost all the samples under investigation exhibit Mn ions in different oxidation states. The temperature variation EPR studies are done and are discussed in view of the paramagnetic oxidation states. The radiation-induced defects have also been assessed by using the EPR spectra of such irradiated food products. 相似文献
6.
S. I. Kuzina A. I. Mikhailov 《Russian Journal of Physical Chemistry A, Focus on Chemistry》2006,80(10):1666-1670
The secondary reactions of the oxidation and thermal transformations of gamma irradiated (at 77 K) and plasticized (with water) cellulose radicals were studied by 3 cm-and 2 mm-band EPR spectroscopy. The radiolysis of cotton cellulose was found to produce the H-C*=O formyl radical, and heating the irradiated samples to 190–200 K resulted in the formation of the ROO* peroxide radical. The EPR spectra of microcrystalline cellulose recorded at room temperature contained an individual triplet (α β H = 2.5–2.7 mT) with an additional quadruplet structure (splitting 0.5–0.7 mT) from three γ-hydrogens. This triplet was interpreted as a signal of the primary radical at C4. The main direction of thermal transformations of primary radicals was synchronous reactions of the dehydration of the polycarbohydrate complex accompanied by the dissociation of the C-H, C-OH, and C-C bonds and elimination of H2O, H2, CO, and CO2 with successive formation of allyl and then polyene radicals, which were a source of the growth of polyconjugated systems in macromolecules. 相似文献
7.
The behavior of the poly(methyl methacrylate) (PMMA) under the action of gamma radiation has been sufficiently studied. In this work, we present results from melt flow index (MFI), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and electron paramagnetic resonance (EPR) of PMMA composites with hydroxyapatite (HA) and seaweed residues (SW) irradiated with gamma rays at 1.08 kGy/h. Composites of PMMA/HA and PMMA/SW with 10%, 20% and 30% of the filler were prepared. The results show an increase in the MFI values with the integral dose of radiation, being consistent with chain-scission reactions. No EPR signal was observed in pure PMMA, while in the composites, the typical EPR signal of the PMMA radicals was observed, which increased with the amount of HA or SW. When comparing the relative intensities of the EPR signals for both types of composites, a slight increase in the concentration of free radicals generated in the sample with SW respect to that of PMMA/HA composite was obtained. A decay of the total free radical concentration was observed as time elapsed. 相似文献
8.
O. G. Duliu M. Ferdes O. S. Ferdes 《Journal of Radioanalytical and Nuclear Chemistry》2004,260(2):273-277
The EPR spectra of three types of amylases (microbial and fungal α-amylase E.C. 3.2.1.1; gluco-amylase E.C. 3.2.1.3) and one
type of pectinase irradiated with gamma-rays and 7 MeV electrons are presented and discussed. For all enzymes, a positive
correlation (r = 0.991 to 0.994) between the EPR signal amplitude and absorbed dose has been observed, while, between EPR spectra amplitudes
and enzyme in vitro activity, in terms of enzymatic activity a negative correlation (r = -0.987 to -0.995), has been noticed. These facts recommend that enzyme damage during irradiation can be monitored by means
of the free radicals detected by EPR spectroscopy.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
9.
C.P. Lakshmi Prasuna R.P.S. Chakradhar J.L. Rao N.O. Gopal 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2009,74(1):140-147
EPR spectral investigations have been carried out on four edible leafy vegetables of India, which are used as dietary component in day to day life. In Rumex vesicarius leaf sample, EPR spectral investigations at different temperatures indicate the presence of anti-ferromagnetically coupled Mn(IV)–Mn(IV) complexes. EPR spectra of Trigonella foenum graecum show the presence of Mn ions in multivalent state and Fe3+ ions in rhombic symmetry. EPR spectra of Basella rubra indicate the presence of Mn(IV)–O–Mn(IV) type complexes. The EPR spectra of Basella rubra have been studied at different temperatures. It is found that the spin population for the resonance signal at g = 2.06 obeys the Boltzmann distribution law. The EPR spectra of Moringa oliefera leaves show the presence of Mn2+ ions. Radiation induced changes in free radical of this sample have also been studied. The FT-IR spectra of Basella rubra and Moringa oliefera leaves show the evidences for the protein matrix bands and those corresponding to carboxylic CO bonds. 相似文献
10.
Nyok-Sai Hon 《Journal of polymer science. Part A, Polymer chemistry》1976,14(10):2513-2525
Free-radical formation in various modifications of celluloses, namely, wood cellulose I, II, III, and IV, rayon cellulose, amorphous cellulose, cotton linters, and absorbent cotton, irradiated with ultraviolet light, has been studied by means of ESR spectroscopy at 77°K. Various types of free radicals were generated from these samples. The line shapes and the signal intensities of the ESR spectra depended greatly upon the degree of crystallinity, the lattice type, and the arrangement of molecules in cellulose. The effect of degree of crystallinity and the amount of sensitizer (Fe3+) absorbed revealed that photochemical reactions in cellulose occurred exclusively in the amorphous regions of the polymer. Free radicals formed in these samples behaved distinctively upon a warm-up process. Phenomena of radical migration and formation of new radicals were observed from the sensitized samples of rayon cellulose and amorphous cellulose. 相似文献
11.
Nyok-Sai Hon 《Journal of polymer science. Part A, Polymer chemistry》1975,13(12):2653-2669
The ESR spectra of untreated and photosensitized celluloses irradiated with three different ultraviolet light sources, i.e., λ > 2537 Å, λ > 2800 Å, λ > 3400 Å, at 77°K under vacuum were studied. Based on the warm-up process, that is, warming the sample from 77°K to 273°K for a certain time and recorded at 77°K, the decay behavior of free radicals of celluloses was examined for changes of the pattern and the intensities of ESR spectra. For the untreated samples irradiated with light of λ > 2537 Å and λ > 2800 Å, beside the two doublet spectra originating from hydrogen atoms (508 gauss splitting) and formyl radicals (129 gauss splitting), the observed sevenline spectrum was resolved to be a superposition of a singlet (ΔHmsl = 16 gauss), a doublet (24 gauss splitting), a triplet (34 gauss splitting), and a quartet (overall width, 88 gauss) spectrum. For the photosensitized samples irradiated with light of λ > 3400 Å, the 1:1:1 three-line spectrum was resolved to be a superposition of a singlet (ΔHmsl = 27 gauss), a doublet (43 gauss splitting), and a triplet (34 gauss splitting) spectrum. The five-line spectra of the photosensitized samples irradiated with light of λ > 2537 Å and λ > 2800 Å were resolved to be a superposition of a singlet (ΔHmsl = 27 gauss), a doublet (43 gauss splitting), and a triplet (34 gauss splitting) spectrum. Based on these findings, the conclusion was drawn that at least six kinds of spectra, generated from six kinds of radical species, were formed in cellulose irradiated with ultraviolet light under appropriate experimental conditions. 相似文献
12.
Angelo Alberti Eugenio Chiaravalle Piergiorgio Fuochi Dante Macciantelli Michele Mangiacotti Giuliana Marchesani Elena Plescia 《Radiation Physics and Chemistry》2011,80(12):1363-1370
High energy radiation treatment of foodstuff for microbial control and shelf-life extension is being used in many countries. However, for consumer protection and information, the European Union has adopted the Directives 1999/2/EC and 1999/3/EC to harmonize the rules concerning the treatment and trade of irradiated foods in EU countries. Among the validated methods to detect irradiated foods the EU directives also include Electron Paramagnetic Resonance (EPR/ESR) spectroscopy.We describe herein the use of EPR for identification of four species of bivalve mollusks, i.e. brown Venus shells (Callista chione), clams (Tapes semidecussatus), mussels (Mytilus galloprovincialis) and oysters (Ostrea edulis) irradiated with 60Co γ-rays. EPR could definitely identify irradiated seashells due to the presence of long-lived free radicals, primarily CO2−, CO33−, SO2− and SO3− radical anions. The presence of other organic free radicals, believed to originate from conchiolin, a scleroprotein present in the shells, was also ascertained. The use of one of these radicals as a marker for irradiation of brown Venus shells and clams can be envisaged. We also propose a dosimetric protocol for the reconstruction of the administered dose in irradiated oysters. 相似文献
13.
Ralitsa B. Mladenova Cyril Firzov Nicola D. Yordanov 《Radiation Physics and Chemistry》2010,79(9):990-993
Nutritive supplements Enoviton, Enoviton C and Enoviton CE containing standardized anthocyanins from lyophilized red wine, vitamins (some of them) and excipients were investigated by EPR spectrometry before and after gamma-irradiation. Non-irradiated samples exhibit one singlet line with g=2.0039±0.0002, most probably due to free radicals from anthocyanins. After irradiation with 10 kGy gamma-rays, tablets of Еnoviton, Еnoviton С and Еnoviton СЕ, all exhibit complex EPR signals centered at a g-value of g=2.0034. The EPR spectrum of irradiated Enoviton is different from that of Еnoviton С or Еnoviton СЕ due to the overlap of the spectra of microcrystalline cellulose and the background singlet spectrum present in all tablets with the EPR resonance due to irradiated ascorbic acid (in Еnoviton С and Еnoviton СЕ). Gamma-induced free radicals exhibit long time stability—for a six months period the intensity of central peak decrease with 30–40%. 相似文献
14.
《Radiation Physics and Chemistry》2009,78(3):213-216
The results of electron paramagnetic resonance (EPR) study on fresh fruits (whole pulp of pears, apples, peaches, apricots, avocado, kiwi and mango) before and after gamma-irradiation are reported using two drying procedures before EPR investigation. In order to remove water from non-irradiated and irradiated samples of the first batch, the pulp of fresh fruits is pressed, and the solid residue is washed with alcohol and dried at room temperature. The fruits of the second batch are pressed and dried in a standard laboratory oven at 40 °C. The results obtained with both drying procedures are compared. All samples under study show a singlet EPR line with g=2.0048±0.0005 before irradiation. Irradiation gives rise to typical “cellulose-like” EPR spectrum featuring one intensive line with g=2.0048±0.0005 and two very weak satellite lines situated 3 mT at left and right of the central line. Only mango samples show a singlet line after irradiation. The fading kinetics of radiation-induced EPR signal is studied for a period of 50 days after irradiation. When the irradiated fruit samples are stored in their natural state and dried just before each EPR measurement, the satellite lines are measurable for less than 17 days of storage. Irradiated fruit samples, when stored dried, lose for 50 days ca. 40% of their radiation-induced radicals if treated with alcohol or ca. 70% if dried in an oven. The reported results unambiguously show that the presence of the satellite lines in the EPR spectra could be used for identification of radiation processing of fresh fruits, thus extending the validity of European Protocol EN 1787 (2000). Foodstuffs—Detection of Irradiated Food Containing Cellulose by EPR Spectroscopy. European Committee for Standardisation. Brussels for dry herbs. 相似文献
15.
Joong-Ho Kwon Jae-Jun Ahn Kashif Akram Ik-Jae Son Sang-O Lee 《Analytical and bioanalytical chemistry》2013,405(12):4225-4234
Two kinds (20 each) of gamma-irradiated (0, 5, and 10 kGy) tea samples, blended powders and packed in sachets (tea bags), were investigated using photostimulated luminescence (PSL), thermoluminescence (TL), and electron spin resonance spectroscopy (ESR) to identify their irradiation status. PSL-based rapid screening was possible for all the control samples except for one packed and two powdered samples. The irradiated samples presented a good dose-dependent PSL count except two powdered samples with very low PSL sensitivity. TL analysis provided the most reliable results, in which all the irradiated samples were identified using a well-defined high-intensity TL glow curve in a temperature range of 150–250 °C. The TL results were also confirmed by determining the TL ratio (TL1/TL2), which was <0.1 in all the non-irradiated samples and >0.1 in the irradiated ones. ESR spectroscopy was effective for only 3 packed and 6 powdered samples showing the radiation-induced cellulosic and sugar radical signals, respectively. Figure
TL-based detection of irradiated teas 相似文献
16.
《Radiation Physics and Chemistry》2007,76(6):1031-1036
The 9.50 GHz electron paramagnetic resonance (EPR) spectra of unirradiated and 60Co γ-ray irradiated cardamom (Elettaria cardamomum L. Maton, Zingiberaceae), ginger ((Zingiber officinale Rosc., Zingiberaceae), and saffron (Crocus sativus L., Iridaceae) have been investigated at room temperature. All unirradiated spices presented a weak resonance line with g-factors around free-electron ones. After γ-ray irradiation at an absorbed dose of up to 11.3 kGy, the presence of EPR spectra whose amplitude increase monotonously with the absorbed dose has been noticed with all spices. A 100 °C isothermal annealing of 11.3 kGy irradiated samples has shown a differential reduction of amplitude of various components that compose initial spectra, but even after 3.6 h of thermal treatment, the remaining amplitude represents no less then 30% of the initial ones. The same peculiarities have been noticed after 83 days storage at room temperature but after 340 days storage at ambient conditions only irradiated ginger displays a weak signal that differs from those of unirradiated sample. All these factors could be taken into account in establishing at which extent the EPR is suitable to evidence any irradiation treatment applied to these spices. 相似文献
17.
In order to obtain detailed insights into the physicochemical mechanism of DNA damage induction, “in situ” measurement of electron paramagnetic resonance (EPR) signal from DNA constituent nucleobases, guanine and adenine, has been performed in a vacuum using monochromatic synchrotron soft X-rays. We found that short-lived unpaired electron species arise only during irradiation to the evaporated thin film on a surface. The EPR spectrum of the short-lived species significantly depends on the photon energy irradiated, and the spin concentration obtained from the EPR spectra shows a similar fine structure to the X-ray photoabsorption spectra (X-ray absorption near edge structure; XANES). For the adenine sample, the spin concentration alters strikingly by water absorption on the sample surface. Trapping of photo- or Auger electrons into a newly generated potential in the nucleobases as the consequence of photoelectric effect is suggested as mechanisms of the induction of the short-lived species. 相似文献
18.
Joong-Ho Kwon Tusneem Kausar Jungeun Noh Dong-Ho Kim Myung-Woo Byun Kyong-Su Kim Kwan-Soo Kim 《Radiation Physics and Chemistry》2007,76(11-12):1833-1836
Dried seasoned filefish (Thamnaconus modestus) was irradiated at 0–10 kGy and the identification of irradiation treatment was investigated by analyzing the characteristics of thermoluminescence (TL), hydrocarbon (HC), and 2-alkylacyclobutanone (2-ACB). The TL (TL1), glow curve of the irradiated samples peaked at approximately 150 °C with high intensity, but that of the non-irradiated samples peaked at about 300 °C with little intensity, thus making it possible to discriminate between irradiated and non-irradiated samples. Moreover, TL ratio (TL1/TL2), through normalization steps, enhanced the reliability of the TL1 results. Six kinds of HCs and three kinds of 2-ACBs quantitatively determined for the samples linearly increased in proportion to irradiation doses. In particular, two HCs like 1-hexadecane and 1,7-hexadecadine, and three ACBs, such as 2-dodecylcyclobutanone, 2-(5′-tetradeceyl)cyclobutanone, and 2-tetradecylcyclobutanone, were identified only in the irradiated samples as radiation-induced markers. 相似文献
19.
Hyun-Kyu Kyung Jae-Jun Ahn Kashif Akram Joong-Ho Kwon 《Radiation Physics and Chemistry》2012,81(8):1224-1226
This research was conducted to find the most suitable parameters to separate minerals from irradiated dried shrimps and mussels (0 and 5 kGy) for thermoluminescence analysis using density separation and modified acid hydrolysis (at 50 °C with continuous agitation) methods. Nonirradiated samples gave TL glow curve of low intensity with peak after 300 °C except dried mussel sample, which gave false positive result. This problem was absent in minerals separated by acid hydrolysis. TL ratios of all nonirradiated samples were <0.1 irrespective of method used for mineral separation. Minerals separated from irradiated samples by density separation showed very high intensity of TL glow peak before 200 °C, where results from irradiated dried shrimp samples were better because of good availability of minerals. The minerals separated from irradiated samples by acid hydrolysis showed slightly low TL intensity and glow curve peak was found at about 200 °C. However, acid hydrolysis method was less laborious and required less sample weight as compared to density separation method. TL ratios of all irradiated samples were >0.1 confirming the quality of minerals on TL discs. 相似文献
20.
S. Atta A. Sattar A. Ahmad I. Ali S.A. Nagra T. Ahmad 《Journal of Radioanalytical and Nuclear Chemistry》2001,250(3):537-540
Chicken and fish have been investigated for the detection of radiation treatment using thermoluminescence (TL) technique. The samples were irradiated by 60Co gamma-source at the absorbed doses of 1, 2, 3, 4 and 5 kGy. TL response of treated and untreated samples in the temperature range of 50–300 °C was measured using TL reader with a temperature profile of 10 °C/s. The results revealed that TL values increased with temperature and maximum signal were obtained at 195 °C, in each case. It was also observed that the TL intensities enhanced with the absorbed doses (1–5 kGy) and the increase was dependent on the absorbed dose. From this study it is concluded that the TL technique is a rapid, simple and promising method for identifying chicken and fish treated with gamma-irradiation. 相似文献