联苯胺-H_2O_2-HRP伏安酶联免疫分析体系测定植物病毒TMV和TRSV

提出了联苯胺－Ｈ２Ｏ２－辣根过氧化物酶（ＨＲＰ）伏安酶联免疫分析体系测定植物病毒烟草花叶病毒（ＴＭＶ）和烟草环斑病毒（ＴＲＳＶ）的新方法。ＨＲＰ标记的羊抗兔酶标记抗体ＩｇＧ－ＨＲＰ可以催化Ｈ２Ｏ２氧化联苯胺的反应,其氧化产物在Ｂｒｉｔｏｎ－Ｒｏｂｉｎｓｏｎ（ＢＲ）缓冲溶液中在－０．６２Ｖ（ＳＣＥ）左右产生灵敏的线性扫描二阶导数伏安峰,可以测定ＩｇＧ－ＨＲＰ。根据ＩｇＧ－ＨＲＰ与植物病毒及其抗血清的免疫反应,可以间接测定植物病毒。本法测定ＴＭＶ的检出限为０．２５ｎｇ／ｍＬ,线性范围为０．２５～５０００ｎｇ／ｍＬ;测定ＴＲＳＶ的检出限为１．５ｎｇ／ｍＬ,线性范围为１．５～３０００ｎｇ／ｍＬ;测定ＴＭＶ烟草病叶澄清液的最高稀释比为１∶１００００。检测灵敏度高于酶联免疫吸附显色光度法（ＥＬＩＳＡ）     

化学发光单孵多层免疫技术检测粪样中轮状病毒

以酶联免疫吸附分析（ＥＬＩＳＡ）夹心测定粪样中轮状病毒（ＲＶ）实验为模型，将传统又孵式免疫操作变成单孵式，即在包被ＲＶ抗体Ａｂ的微孔中同时加入含ＲＶ的粪样上清液和根过氧化物酶（ＨＲＰ）标记抗ＲＶ抗原共同孵育，其结果在载体表面形成多层酶免疫复合物，以ＨＲＰ催化鲁米诺－对碘苯酚－过氧化氢化学体系作为最终信号检测系统，从而建立起化学发光单孵多层免疫技术（ＣＬ－ＳＩＭＩＴ）。其灵敏度是ＥＬＩＳＡ的１６倍，     

STRUCTURAL EVIDENCE FOR THE METAL ION BRIDGED LIGANDLIGAND INTERACTION：THE X－RAY CRYSTAL STRUCTURE OF ［（2，2＇－BIPYRIDYL）（PHENYLMALONATE）COPPER（Ⅱ）］ TRIHYDRATE

ＳＴＲＵＣＴＵＲＡＬＥＶＩＤＥＮＣＥＦＯＲＴＨＥＭＥＴＡＬＩＯＮＢＲＩＤＧＥＤＬＩＧＡＮＤＬＩＧＡＮＤＩＮＴＥＲＡＣＴＩＯＮ：ＴＨＥＸ－ＲＡＹＣＲＹＳＴＡＬＳＴＲＵＣＴＵＲＥＯＦ［（２，２＇－ＢＩＰＹＲＩＤＹＬ）（ＰＨＥＮＹＬＭＡＬＯＮＡＴＥ）ＣＯＰ...     

THE STACKING INERACTION OF TERNARY COMPLEXES OF Zn(ATP)~(2-)AND PYRIDINE-LIKELIGANDS

ＴＨＥ　ＳＴＡＣＫＩＮＧ　ＩＮＥＲＡＣＴＩＯＮ　ＯＦ　ＴＥＲＮＡＲＹ　ＣＯＭＰＬＥＸＥＳ　ＯＦ　Ｚｎ（ＡＴＰ）~（２－）ＡＮＤ　ＰＹＲＩＤＩＮＥ－ＬＩＫＥＬＩＧＡＮＤＳＴＨＥＳＴＡＣＫＩＮＧＩＮＴＥＲＡＣＴＩＯＮＯＦＴＥＲＮＡＲＹＣＯＭＰＬＥＸＥＳＯ?..     

邻苯二胺—过氧化氢—辣根过氧化物酶酶联免疫示差脉冲伏安法测定人血清…

建立了邻苯二胺（ＯＰＤ）－Ｈ２Ｏ２－辣根过氧化物酶（ＨＲＰ）酶联示差脉冲伏安分析体系并用于测定人血清中类风湿因子（ＲＦ），ＨＲＰ催化Ｈ２Ｏ２氧化ＯＰＤ所形成麦催化产物在ＰＨ２．０磷酸盐－枸椽酸缓冲溶液中于－０．１８Ｖ左右产生－灵敏示差脉冲伏安峰，在ＲＦ浓度在１．２５－２０．０Ｕ／ｍＬ之间与峰电流呈线性关系，应用此峰检测人血清ＲＦ的检测限低至０．２８Ｕ／ｍＬ。该法较相同条件下ＥＬＩＳＡ以光度测定法的     

邻苯二胺-过氧化氢-辣根过氧化物酶酶联免疫示差脉冲伏安法测定人血清类风湿因子抗体

建立了邻苯二胺（ＯＰＤ）－Ｈ２Ｏ２－辣根过氧化物酶（ＨＲＰ）酶联示差脉冲伏安分析体系并用于测定人血清中类风湿因子（ＲＦ），ＨＲＰ催化Ｈ２Ｏ２氧化ＯＰＤ所形成酶催化产物在ｐＨ２．０磷酸盐－枸橼酸缓冲溶液中于－０．１８ Ｖ左右产生一灵敏示差脉冲伏安峰，ＲＦ浓度在１．２５～２０．０ Ｕ／ｍＬ之间与峰电流呈线性关系，应用此峰检测人血清ＲＦ的检测限低至 ０．２８Ｕ／ｍＬ。该法较相同条件下ＥＬＩＳＡ显色光度测定法的灵敏度增加５倍，且受干扰较少。     

A NEW N－FORMYL C_（19）－DITERPENOID ALKALOID， ALDOHYPACONITINE， FROM CULTIVATED ACONITUM CARMICHAELI， DEBX．

ＡＮＥＷＮ－ＦＯＲＭＹＬＣ_（１９）－ＤＩＴＥＲＰＥＮＯＩＤＡＬＫＡＬＯＩＤ，ＡＬＤＯＨＹＰＡＣＯＮＩＴＩＮＥ，ＦＲＯＭＣＵＬＴＩＶＡＴＥＤＡＣＯＮＩＴＵＭＣＡＲＭＩＣＨＡＥＬＩ，ＤＥＢＸ．￥ＸｉａｎＫａｉＷＡＮＧ；ＴｏｎｇＦａｎｇＺＨＡＯａｎｄＳｈ?..     

LIGAND EXCHANGE RESIN FOR AMINO ACID RACEMATES

ＬＩＧＡＮＤＥＸＣＨＡＮＧＥＲＥＳＩＮＦＯＲＡＭＩＮＯＡＣＩＤＲＡＣＥＭＡＴＥＳＹｕａｎＺｈｉ；ＨｅＢｉｎｇｌｉｎ（ＩｎｓｔｉｔｕｔｅｏｆＰｏｌｙｍｅｒＣｈｅｍｉｓｔｒｙ，ＮａｎｋａｉＵｎｉｖｅｒｓｉｔｙ，Ｔｉａｎｊｉｎ３０００７１）Ａｂｓｔｒａｃｔ...     

A NEW CYCLOPEPTIDE FROM VACCARIA SEGETALIS

ＡＮＥＷＣＹＣＬＯＰＥＰＴＩＤＥＦＲＯＭＶＡＣＣＡＲＩＡＳＥＧＥＴＡＬＩＳ￥ＲｏｎｇＰｉｎｇＺＨＡＮＧ；ＣｈｅｎｇＺＯＵ；ＹｕＫｕｎＣＨＡＩ；ＪｕｎＺＨＯＵ（ＬａｂｏｒａｔｏｒｙｏｆＰｈｙｔｏｃｈｅｍｉｓｔｒｙ，ＫｕｎｍｉｎｇＩｎｓｔｉｔｕｔｅｏＦｂ...     

对氟苯酚-H_2O_2-HRP体系酶联荧光免疫分析研究ⅢF~--Al-酸性铬蓝K荧光熄灭法测定人血清中乙肝E抗原

建立了一种检测人血清中乙肝Ｅ抗原的新荧光光度法．通过酶促反应：对氟苯酚＋Ｈ２Ｏ２ＨＲＰ→苯酚＋Ｆ－＋Ｈ２Ｏ与Ａｌ－酸性铬蓝Ｋ荧光体系相偶合，测定辣根过氧化物酶（ＨＲＰ）及其标记物．测定ＨＲＰ的线性范围为０．１９～３１ｍＵ／ｍＬ，检出限为０．０４ｍＵ／ｍＬ．     

电致化学发光法测定Mn2+含量的研究

在H3 PO4溶液中,利用Ag+存在条件下,Mn2+可以被电化学氧化成KMnO4,KMnO4进而与酸性邻苯二酚或碱性鲁米诺等发生反应,得到较好的化学发光信号,构建了一种间接测定水溶液中Mn2+含量的方法.研究了Ag+对Mn2+电化学氧化的影响.Mn2+电化学氧化过程中,当Ag+浓度为1.5×10-5 mol/L,H3 PO4浓度为0.01 mol/L,电解2 min后,发光强度达到最大.探讨了不同pH条件下的化学发光强度与Mn2+浓度的关系及选择性.在酸性介质中,邻苯二酚的发光强度与Mn2+在1.82×10-7~7.27×10-5mol/L的浓度范围内呈现良好的线性关系;常见的金属离子等对Mn2+测定干扰小.将本方法用于地表水及饮用水中Mn2+的测定,与标准方法相比,结果令人满意.     

流动注射-化学发光分析法的发展

对流动注射一化学发光分析法的发展作了评述,特别叙述了在此领域中的研究工作的进展,对此方法的基本原理也作了简要介绍（引用文献67篇）。     

Europium(III)-Sensitized Chemiluminescence Determination of Ibuprofen in Pharmaceutical Preparations and Biological Fluids

Abstract

Chemiluminescence reaction of the system containing europium(III) ion, KMnO₄, Na₂SO₃, and ibuprofen was investigated for the determination of ibuprofen. The introduction of Eu(III) ion into the system of KMnO₄-Na₂SO₃-ibuprofen caused a significant increase in the chemiluminescence signal. The increment of the chemiluminescence signal is proportional to ibuprofen concentration in the range of 5.0 × 10^?8–5.0 × 10^?6 g/ml with a detection limit of 1 × 10^?8 g/ml. The relative standard deviation for 1.0 × 10^?7 g/ml ibuprofen solution was 1.7% (n = 11). The proposed method was successfully applied to determine ibuprofen in tablets and human plasma.     

Development of an Ultra-sensitive Chemiluminescence Enzyme Immunoassay for the Determination of Diethylstilbestrol in Seafood

An ultra-sensitive indirect competitive chemiluminescence enzyme immunoassay was developed for screening diethylstilbestrol in fish and shrimp samples. The concentration of diethylstilbestrol that caused 50% inhibition of the binding enzyme marker (IC₅₀) was 0.32 ng/mL and the limit of detection was 0.0068 ng/mL; the linear range was from 0.028 ng/mL to 3.60 ng/mL. The assay showed cross-reactivity of 7.1% and 2.8% with dienestrol and hexoestrol, respectively, but negligible cross-reactivity with estradiol, estrone, ethinyloestradiol, and progestin. The recovery from spiked fish and shrimp samples varied from 68.5% to 92.5%, and the mean coefficients of variation within groups and between groups were 6.2% and 8.0%, respectively. Our results indicated that the assay is a simple, sensitive, specific, and accurate method for screening fish and shrimp samples for diethylstilbestrol.     

Electrogeneration of ferrate (VI) in low concentration NaOH solution for flow injection–chemiluminescence detection

The possibility of direct analytical applications of ferrate(VI) solution, which was freshly electrogenerated in low-concentration NaOH electrolyte, was studied by a flow-injection chemiluminescence (FI–CL) system. It was found that some inorganic ions, organic molecule and biomolecule could enhance the chemiluminescence emission caused by ferrate(VI)–luminol reaction. V(V), Ca(II), Mg(II), phloroglucinol, and bovine hemoglobin (Hb) chosen as samples were successfully detected by this developed method. The analytical characteristics of the system for the analytes determination including linear ranges, correlation coefficients, limits of detection combined with FI analysis were studied.     

发光分析

评述在 2 0 0 1年 1月～ 2 0 0 2年 12月间我国发光分析的发展概况 ,内容包括荧光、磷光、化学发光和电化学发光分析等。引用文献 32 9篇。     

Reversed Flow Injection Chemiluminescence Determination of Pyridoxine Hydrochloride Based on the Cerium(IV)-Sodium Sulfite System

A rapid chemiluminescence (CL) reaction was observed when Ce(IV) was injected into the mixed solution of pyridoxine hydrochloride and sodium sulfite. A new flow-injection CL method for the determination of pyridoxine hydrochloride was established. The detection limit (3σ) was 0.5 mg/L pyridoxine hydrochloride and the relative standard deviation (RSD) was 3.3% (n = 11, γ = 5 mg/L). The CL intensity responded linearly to the concentration of pyridoxine hydrochloride in the ranges 1.0–10.0 mg/L and 10.0–100.0 mg/L with linear correlation coefficients of 0.9994 and 0.9991, respectively. The method had been applied to the determination of pyridoxine hydrochloride in tablets and injections successfully.     

Chemiluminescence immunoassay for chloramphenicol

In recent years, various chemiluminescent clinical immunoassay kits have been widely applied to the detection of hormones. However, a kit for chloramphenicol (CAP) is often absent from most commercial product lists, even though it is important to control the levels of CAP residues in foodstuffs too. Therefore, we describe a simple, solid-phase chemiluminescence immunoassay (CLIA) for the measurement of CAP in foodstuffs. A rabbit anti-CAP IgG is passively adsorbed onto the walls of polypropylene plates. The labeled antigen is horseradish peroxidase (HRP) conjugate of CAP. Luminol solution is used as the substrate of HRP. The light yield is inversely proportional to the concentration of CAP. The method has a similar sensitivity (0.05 ng/ml), specificity, precision, and accuracy to a conventional enzyme immunoassay (EIA). The intra-assay and inter-assay CVs of ten samples were <8% and <20%, respectively, and the analytical recovery of the method was 87–100%. The experimental correlation coefficient of dilution was found to be 0.999 using milk supernatant as buffer. The detection limit for the method was 0.1–10 ng/ml, and it displayed good linearity.     

Determination of ketotifen by using calcein as chemiluminescence reagent

Chemiluminescence (CL) was observed when potassium hexacyanoferrate(III) reacted with the mixture of calcein and ketotifen. Interestingly, the CL intensity would be enhanced by trace amounts of Mg²⁺ and the CL intensity was strongly dependent on ketotifen concentration. Based on this phenomenon, a flow injection CL method was established for the determination of ketotifen. The possible CL mechanism is proposed based on the kinetic characteristic of the CL reaction, CL spectrum, ultraviolet (UV) spectra and fluorescent spectra. The CL intensity was correlated linearly with concentration of ketotifen over the range of 6.0 × 10⁻⁹ to 2.0 × 10⁻⁷ g mL⁻¹ and the detection limit was 3 × 10⁻⁹ g mL⁻¹. The relative standard deviation was 1.8% for 2.0 × 10⁻⁸ g mL⁻¹ ketotifen (n = 11). This method was applied to the determination of ketotifen in the tablets successfully.     

FI automatic method for the determination of copper(II) based on coproporphyrin I-Cu(II)/TCPO/H(2)O(2) chemiluminescence reaction for the screening of waters

In this paper, an automatic method for the screening of water samples containing Cu(II) was proposed, based on peryoxalate chemiluminescence reaction using coproporphyrin I as fluorophor compound to provide selectivity and a simple flow injection (FI) chemiluminescence detector (CLD). FI system conditions were chosen in order to distinguish samples over or under legislation limit established (50 μg l⁻¹) with high reliability. The detection limit found was 9 μg l⁻¹ and the linear dynamic range was 15-125 μg l⁻¹ of Cu(II). Repeatibility and reproducibility studies gave good precision and accuracy with recovery near 100%. Under these conditions, the method resulted selective and only Fe(II), Fe(III) and Pb(II) could interfere, but at a concentration level higher than their normal concentration in waters. The proposed method was found to be highly reliable for screening purposes and it was successfully applied to the screening of a variety of real water samples.