共查询到19条相似文献,搜索用时 66 毫秒
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研究了铕-敌鼠-DL-组氨酸-CTMAB荧光体系的荧光特性,测定了体系的影响因素和测定敌鼠的实验方法,体系的激发和发射波长分别为330nm和612nm,各组分的最佳浓度分别为1.0×10^-5mol/L,DL-组氨酸,1.0×10^-4mol/L,CTMAB1.0×10^-5mol/L,pH为9.0,在此条件下,敌鼠浓度在6.0×10^-7~9.0×10^-5mol/L范围内同体系的荧光强度成线性 相似文献
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桑色素——TritonX—100—十二烷基硫酸钠荧光光度法测定痕量铍 总被引:1,自引:0,他引:1
用荧光光度法研究了桑色素-TritonX-100-十二烷基硫酸钠-Be(Ⅱ)体系的测定条件及方法,在pH12.5的硼酸-氢氧化钠缓冲溶液中,铍含量在0~48ng.ml^-1范围内呈良好的线性关系,其检出限为0.031ng.ml^-1。方法具有灵敏度高,选择性好,可不经有机溶剂萃取,于水相中直接对铝合金,铜片及河水等样品中的痕量铍进行测定。 相似文献
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PhenNO—TTA—乳化剂OP荧光光度法测定微量铕 总被引:3,自引:0,他引:3
研究了Eu(Ⅲ)-PhenNO-TTA-乳化剂OP体系的荧光性质及其用于微量铕的测定。该体系具有良好的分析特性,最低检测限可达7.0×10^-14mol/L. 相似文献
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反相高效液相色谱法同时检测食物中毒样品中的敌鼠钠盐、杀鼠醚和杀鼠灵 总被引:1,自引:0,他引:1
建立了反相高效液相色谱-二极管阵列检测器同时快速检测食物中毒样品中的敌鼠钠盐、杀鼠醚和杀鼠灵的分析方法。固体样品用乙腈提取,液体样品用乙酸乙酯提取。以V(乙腈)∶V(0.010 mol/L乙酸铵)=57∶43(pH 4.50)为流动相,经C18色谱柱分离,二极管阵列检测器分别在波长286和306 nm处同时检测,在8 min内即可完成色谱分析。敌鼠钠盐、杀鼠醚和杀鼠灵在0.05~20.0μg/mL范围内与其色谱峰面积呈良好的线性关系,方法的检出限固体样品为0.015μg/g,液体样品为0.003μg/mL,样品加标平均回收率在85.0%~109.2%之间,相对标准偏差为1.0%~9.6%。 相似文献
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铕—钆—二苯甲酰甲烷—氨体系荧光增强效应的研究 总被引:1,自引:0,他引:1
报道了Eu-DBM-NH_3的溶剂效应和共发光效应。甲醇、丙酮和乙醇等溶剂对该体系有明显的增敏作用;Gd~(3+)、Y~(3+)、Tb~(3+)、La~(3+)和Lu~(3+)等可显著增强其荧光强度,并以Gd~(3+)效果最好。该体系可用于超痕量铕的测定,检出限为3.0×10~(-11)mol/L,比无Gd~(3+)时约降低2个数量级。 相似文献
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FluorescenceEnhancementofSamarium-Thenoyl-trifluoroacetone-CetyltrimethylammoniumBromide-TritonX-100byScandiumZHUGui-yun,LIUP... 相似文献
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《Analytical letters》2012,45(12):2237-2250
Abstract An ultrasensitive electrochemical immunoassay based on biocatalytic deposition has been proposed for the detection of Schistosoma japonicum antibody (SjAb) in infected rabbit serum. Schistosoma japonicum antigen (SjAg) was immobilized on the gold electrode surface via glutaraldehyde crosslink and then incubated with infected rabbit serum containing SjAb; finally, the goat anti-rabbit IgG labeled with alkaline phosphatase was sandwiched to form the immunocomplex on the gold electrode surface. The alkaline phosphatase converted nonelectroactive substrate into the reducing agent and the latter, in turn, reduced metal ions to form electroactive metallic product on the electrode surface. Linear sweep voltammetry (LSV) was used to quantify the amount of the deposited silver and give the analytical signal for SjAb. Assay conditions such as the antigen concentration and enzymatic silver deposition time were optimized. The electrochemical immunosensor was able to realize a reliable determination of SjAb in the dilution range from 1:5000 to 1:100 with a detection limit of 1:6457 of dilution ratio. The feasibility of the proposed immunosensor for possible clinical applications was also investigated by analyzing real serum samples. 相似文献
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《Analytical letters》2012,45(5):945-951
Abstract The fluorescence enhancement of the Eu-dibenzoylmethane(DBM)- cetylpyridinium-chloride (CPC) system was studied. The fluorescence intensity of the system can be greatly increased by Gd3+ in the presence of triethanolamine(TEA). The optimum conditions for enhanced fluorescence of the Eu-Gd-DBM-CPC system were examined. It was a linear function of the Eu3+ concentration in the range of 1.0×10?10~1.0×10?8 mol dm?3 under the optimized conditions. The detection limit was 2.6 × 1011 mol dm?3 in an aqueous solution at pH=7.5~9.5. The Eu-Gd-DBM-CPC system was applied to the determination of europium in rare earth oxides with satisfactory results. The mechanism of enhanced fluorescence was discussed. 相似文献
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稀土离子对铕-2-噻吩甲酰三氟丙酮-氯化甲基三烷基铵-Triton X-100荧光体系的共发光能力的比较 总被引:1,自引:0,他引:1
本文研究了稀土离子对铕(Eu)-2-噻吩甲酰三氟丙酮(TTA)-氯化甲基三烷基铵(N263)-Triton X-100体系的共发光能力,提出用放大倍数作为共发光能力比较的定量标准,用能量转移效率和能量利用率来预测稀土离子的共发光能力。该方法适用于不同条件或不同体系之间共发光能力的定量比较。 相似文献
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The study of fluorescence enhancement has attracted attention recently. For example, the sensitivity and selectivity of Eu-thenoyltrifluoroacetone (TTA)phenan-throline and Eu-diphacinone-ammonia systems in Triton X-100 micelles are increased by the addition of La~(3+) Gd~(3+), Tb~(3+), Lu~(3+) or Y~(3+). The mechanism of this fluorescence(?) 相似文献
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测钼的荧光熄灭法多采用钼与罗丹明类荧光试剂生成不发荧光的配合物而进行测定,我们最近合成的5′-硝基-水杨基荧光酮(5′-N-SAF)结构式见右图,当水溶液中存在TritonX-100时,该配合物具有强烈的荧光;痕量钼的存在可使荧光减弱,据此建立了荧光熄灭测定痕量钼的新方法,该法的灵敏度高(检测限达4×10-3μg/mL)、选择性好、简单、快速,优于文献,本文研究了Mo(Ⅵ)--5′-N-SAF配合物的形成条件。 相似文献
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The Eu-benzoylacetone-phenanthroline system, which has strong fluorescence intensity, was studied. It was found that the fluorescence intensity can be greatly increased by La, Gd, Tb, Lu and Y, Tb giving the greatest enhancement (ca. two orders of magnitude). The maximum fluorescence intensity was obtained at pH 8.0. Beer's law was obeyed from 1.0 × 10?9 to 2.0 × 10?7 M Eu. The detection limit for europium was 2.0 × 10?11 M. 相似文献