Effect of Sample Pre-Contact on the Experimental Evaluation of Cartilage Mechanical Properties

Mechanical characterization of articular cartilage has been widely analyzed in the literature, both experimentally and numerically, since load support (strength) and deformability (compliance) are among the most important physiological functions of this living tissue. They are also important indicators of cartilage degradation and regeneration. Cartilage elastic properties (Young’s Modulus, Aggregate Modulus and Poisson ratio) are usually determined experimentally by uniaxial confined and unconfined compression tests. Relevant differences can be found in the literature for these variables even when similar experimental protocols are used. Before starting the actual compression test, a pre-contact protocol has to be carried out in order to obtain optimum contact between the test tool and the cartilage sample. The aim of this work is to analyze the effect of sample pre-contact on the test results comparing three different protocols available in the literature (predeformation of 4 and 10 % of the total thickness of the sample and a preload equivalent to 12.5 KPa). The implementation of these protocols achieved contact, but only the predeformation allowed normalized experiments and ensured repeatability of the tests. Additionally, under the predeformation protocols, the results and trends for uniaxial compression tests were consistent with the cartilage physiological function. However, for 10 % predeformation, the experimental data showed maximum dispersion at the final stress levels. The statistical treatment determined significant differences between the 10 % predeformation and the preload results (Aggregate Modulus and Poisson ratio), defining them as non comparable protocols. Finally, a new pre-contact protocol is proposed introducing an initial approximation point between the test tool and the cartilage sample, and followed by a predeformation of 4 % for confined and unconfined tests.     

Rheological behaviour of vitreous humour

The vitreous humour (VH) is a complex biofluid that occupies a large portion of the eyeball between the lens and the retina, and exhibits non-Newtonian rheological properties that are key for its function in the eye. It is often possible to distinguish two different phases in VH, known as liquid and gel phases (Sebag J Eye 1: 254–262, 1987). In this work, we present a detailed rheological characterisation of the two phases of the VH under shear and extensional flow conditions. Healthy New Zealand rabbit eyes were used to measure the surface tension and the shear and extensional rheological properties of VH in different phase conformations and at different times after dissection. The results show that VH liquid phase exhibits a surface tension of 47.8 mN/m, a shear thinning behaviour reaching a viscosity plateau around 10^?3 Pa s for shear rates above ~1000 s^?1, and an average relaxation time of 9.7 ms in extensional flow. Interestingly, both VH phases present higher storage modulus than loss modulus, and the measurements performed with VH gel phase 4?±?1 h after dissection exhibit the highest moduli values. The compliance measurements for the gel phase show a viscoelastic gel behaviour and that compliance values decrease substantially with time after dissection. Our results show that the two VH phases exhibit viscoelastic behaviour, but with distinct rheological characteristics, consistent with a gel phase mostly composed of collagen entangled by hyaluronan and a second phase mainly composed of hyaluronan in aqueous solution.     

Large Strain Shear Compression Test of Sheet Metal Specimens

A hybrid experimental-computational procedure to establish accurate true stress-plastic strain curve of sheet metal specimen covering the large plastic strain region using shear compression test data is described. A new shear compression jig assembly with a machined gage slot inclined at 35° to the horizontal plane of the assembly is designed and fabricated. The novel design of the shear compression jig assembly fulfills the requirement to maintain a uniform volume of yielded material with characteristic maximum plastic strain level across the gage region of the Shear Compression Metal Sheet (SCMS) specimen. The approach relies on a one-to-one correlation between measured global load–displacement response of the shear compression jig assembly with SCMS specimen to the local stress-plastic strain behavior of the material. Such correlations have been demonstrated using finite element (FE) simulation of the shear compression test. Coefficients of the proposed correlations and their dependency on relative plastic modulus were determined. The procedure has been established for materials with relative plastic modulus in the range 5?×?10^?4?<?(E _p /E)?<?0.01. It can be readily extended to materials with relative plastic modulus values beyond the range considered in this study. Nonlinear characteristic hardening of the material could be established through piecewise linear consideration of the measured load–displacement curve. Validity of the procedure is established by close comparison of measured and FE-predicted load–displacement curve when the provisional hardening curve is employed as input material data in the simulation. The procedure has successfully been demonstrated in establishing the true stress-plastic strain curve of a demonstrator 0.0627C steel SCMS specimen to a plastic strain level of 49.2 pct.     

Mesoscale modeling of nonlinear elasticity and fracture in ceramic polycrystals under dynamic shear and compression

Dynamic deformation and failure mechanisms in polycrystalline ceramics are investigated through constitutive modeling and numerical simulation. Two ceramics are studied: silicon carbide (SiC, hexagonal crystal structure) and aluminum oxynitride (AlON, cubic crystal structure). Three dimensional finite element simulations incorporate nonlinear anisotropic elasticity for behavior of single crystals within polycrystalline aggregates, cohesive zone models for intergranular fracture, and contact interactions among fractured interfaces. Boundary conditions considered include uniaxial strain compression, uniaxial stress compression, and shear with varying confinement, all at high loading rates. Results for both materials demonstrate shear-induced dilatation and increasing shear strength with increasing confining pressure. Failure statistics for unconfined loading exhibit a smaller Weibull modulus (corresponding to greater scatter in peak failure strength) in AlON than in SiC, likely a result of lower prescribed cohesive fracture strength and greater elastic anisotropy in the former. In both materials, the predicted Weibull modulus tends to decrease with an increasing number of grains contained in the simulated microstructure.     

Viscoelastic analysis of single-component and composite PEG and alginate hydrogels简

Hydrogels, three-dimensional hydrophilic polymer networks, are appealing candidate materials for study- ing the cellular microenvironment as their substantial water content helps to better mimic soft tissue. However, hydrogels can lack mechanical stiffness, strength, and tough- ness. Composite hydrogel systems have been shown to improve upon mechanical properties compared to their single- component counterparts. Poly （ethylene glycol） dimethacrylate （PEGDMA） and alginate are polymers that have been used to form hydrogels for biological applications. Single- component and composite PEGDMA and alginate systems were fabricated with a range of total polymer concentrations. Bulk gels were mechanically characterized using spherical indentation testing and a viscoelastic analysis framework. An increase in shear modulus with increasing polymer con- centration was demonstrated for all systems. Alginate hydro- gels were shown to have a smaller viscoelastic ratio than the PEGDMA gels, indicating more extensive relaxation over time. Composite alginate and PEGDMA hydrogels exhib- ited a combination of the mechanical properties of the con- stituents, as well as a qualitative increase in toughness. Additionally, multiple hydrogel systems were produced that had similar shear moduli, but different viscoelastic behaviors. Accurate measurement of the mechanical properties of hydrogels is necessary in order to determine what parameters are key in modeling the cellular microenvironment.     

Characterization of Anisotropic Polymeric Foam Under Static and Dynamic Loading

An orthotropic polymeric foam with transverse isotropy (Divinycell H250) used in composite sandwich structures was characterized at various strain rates. Uniaxial experiments were conducted along principal material axes as well as along off-axis directions under tension, compression, and shear to determine engineering constants, such as Young??s and shear moduli. Uniaxial strain experiments were conducted to determine mathematical stiffness constants, i. e., C _ij . An optimum specimen aspect ratio for these tests was selected by means of finite element analysis. Quasi-static and intermediate strain rate tests were conducted in a servo-hydraulic testing machine. High strain rate tests were conducted using a split Hopkinson Pressure Bar system built for the purpose using polymeric (polycarbonate) bars. The polycarbonate material has an impedance that is closer to that of foam than metals and results in lower noise to signal ratios and longer loading pulses. It was determined by analysis and verified experimentally that the loading pulses applied, propagated along the polycarbonate rods at nearly constant phase velocity with very low attenuation and dispersion. Material properties of the foam were obtained at three strain rates, quasi-static (10^?4 s^?1), intermediate (1 s^?1), and high (10³ s^?1) strain rates. A simple model proposed for the Young??s modulus of the foam was in very good agreement with the present and published experimental results.     

In situ determination of adhesive shear moduli using strain gages

A new, convenient and cost-effective method of determining in situ adhesive shear moduli using strain gages is proposed and evaluated. Thick-adherend lap shear specimens with stacked gage rosettes at the center of the bond line are loaded in tension for adhesive shear strain measurement. Experimental and numerical results indicate that the test specimen has a nonuniform adhesive shear stress (or strain) distribution in the test section and that this distribution (except at the center point of the bond line) is greatly affected by load eccentricity. In addition to the nonuniformity in the shear stress distribution, the issue of material nonhomogeneity in the gage-covered region affects the strain gage measurement. By taking into account these two issues and assuming linear-elastic behavior, two approaches for converting the gage-measured shear strain into the adhesive shear strain are developed and verified by experiment. It is shown that the strain gage measurement associated with either of two conversion techniques can determine the in situ adhesive shear moduli, which are comparable with moiré experiment and KRG-1 extensometer measurements.     

Identification of Damping Characteristic of a Structural Adhesive by Extended Modal Based Direct Model Updating Method

In order to identify damping characteristics (loss factors in bending and shear modes versus frequency) of an adhesive material, modal based direct model updating method used in previous paper (Jahani and Nobari, Exp Mech, 48:2008) is modified here. Two approaches adopted in the identification process. In the first one, the pre identified moduli from undamped model were used in identification of loss factors and in the second approach loss factors and moduli are identified simultaneously. The loss factors of a typical adhesive were identified using both approaches leading to acceptable results in both cases. The results show that loss factors and Young’s and shear moduli of adhesive are frequency dependent. All experiments and subsequent identifications are conducted both in bending and shear modes. The repeatability and consistency of method is proved by repeating the identification process for several times.     

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对具有不同拉压模量的厚壁球壳,采用双剪统一强度理论推导了其扩张问题的应力及位移的 统一解. 分析了不同模量、不同模型控制参数对厚壁球壳扩张时的扩张压力和应力场的影响. 结果表明：厚壁球壳弹性极限压力、应力场、位移场等均随着模量控制参数、模型参数的变 化而变化,在$\alpha<1$的情况下(即$E^ + < E^ - $),可以明显提高球壳的弹 性极限压力$p_e $; 厚壁球壳塑性极限压力与材料的拉压模量无关,与模型参数$\eta $有关,且随$\eta$的增加,先增大后减小. 因此若采用经典的弹性理论和单一的 模型参数对厚壁球壳进行设计计算,会带来较大的误差.     

High-strain-rate tests on titanium 6-6-2 utilizing a unique rate-testing machine

This paper presents the design of a unique materials-testing system capable of medium strain rates of from 10^?4 to 10²/s. The design incorporates both closed-loop hydraulic operation with that of open-loop pneumatic operation. A novel design permits accurate specimen alignment and a stiff frame which exceeds 17×10⁶ lb/in. (11.7×10⁴ MPa). The mechanine is able to perform conventional tension/compression tests, fatigue tests and, with slight modification, biaxial-stress-tube tests and triaxial-stress tests. The accurate alignment capability coupled with high frame stiffness and the pneumatic operation enables the testing of brittle materials with rigid grips. Titanium 6-6-2 was tested in both tension and compression at strain rates from 10^?4 to about 10/s at four selected temperatures. The material showed a slight strain-rate sensitivity. Yield stress was shown to increase with strain rate while ductility decreased at each test temperature.     

Scale effects on nonlocal buckling analysis of bilayer composite plates under non-uniform uniaxial loads

Scale effects are studied on the buckling behavior of bilayer composite plates under non-uniform uniaxial compression via the nonlocal theory. Each isotropic plate is composed of a material that is different from others, and the adhesive between the plates is modeled as the Winkler elastic medium. According to the symmetry, effects of the Winkler non-dimensional parameter, the thickness ratio, the ratio of Young''s moduli, and the aspect ratio are also considered on the buckling problem of bilayer plates, where only the top plate is under the uniaxial compression. Numerical examples show that the Winkler elastic coefficient, the thickness ratio, and the ratio of Young''s moduli play decisive roles in the buckling behavior. Nonlocal effect is significant when the high-order buckling mode occurs or the aspect ratio is small.     

Identification of Dynamic (Young’s and Shear) Moduli of a Structural Adhesive Using Modal Based Direct Model Updating Method

In this paper, mechanical characteristics (Young’s modulus and shear modulus) of an adhesive are identified using modal based direct model updating method and experimental modal data. The results show that both Young’s and shear moduli of adhesive are frequency dependent. Also, it is demonstrated that the thickness and length of the adhesive-line have influence on these properties. All experiments and subsequent identifications are conducted both in bending and shear modes, and it has been shown that the shear modulus of adhesive is more sensitive to length and thickness variations. The repeatability and consistency of method is proved through repeating the process several times and with different adherends.     

Strain-Rate-Dependent Failure of a Toughened Matrix Composite

The strain-rate-dependent behavior of a toughened matrix composite (IM7/8552) was characterized under quasi-static and dynamic loading conditions. Unidirectional and off-axis composite specimens were tested at strain rates ranging from 10^?4 to 10³ s^?1 using a servo-hydraulic testing machine and split Hopkinson pressure bar apparatus. The nonlinear response and failure were analyzed and evaluated based on classical failure criteria and the Northwestern (NU) failure theory. The predictive NU theory was shown to be in excellent agreement with experimental results and to accurately predict the strain-rate-dependent failure of the composite system based on measured average lamina properties.     

压缩-剪切复合应力下PMMA材料的静态力学特性研究

通过压缩具有一定倾斜角(0°,10°,15°,20°和25°)试件和双剪切模型试件,实现了单轴压缩、压缩-剪切复合应力以及纯剪切三种应力状态,得到PMMA(聚甲基丙烯酸甲酯)在相应应力状态下的应力-应变曲线,同时对不同应力状态下试件的破坏模式进行了分析。结果表明:在不同受力环境中材料的强度和破坏的机理不同;同单轴压缩状态下相比,材料在压缩-剪切复合应力状态下屈服极限、强度极限以及破坏应变均不同程度的增大,呈现明显的"剪切增强"现象。单轴压缩与压缩-剪切应力状态下试件的破坏模式均为在试件短对角面上出现明显的剪切屈服带,由应力分析得出试件剪应力在短对角面上达到最大,引起在此平面上分子链间滑动从而产生应变软化形成剪切屈服带;双剪切试件的破坏模式为与剪切面呈45°的斜面。     

Annular Pulse Shaping Technique for Large-Diameter Kolsky Bar Experiments on Concrete

The goal of this study is to design a novel annular pulse shaping technique for large-diameter Kolsky bars for investigating the dynamic compressive response of concretes. The purpose of implementing an annular pulse shaper design is to alleviate inertia-induced stresses in the pulse shaper material that would otherwise superpose unwanted oscillations on the incident wave. This newly developed pulse shaping technique led to well-controlled testing conditions enabling dynamic stress equilibrium, uniform deformation, and constant strain-rate in the testing of a chosen concrete material. The observed dynamic deformation rate of the concrete is highly consistent (8 % variation) with the stress in the specimen well equilibrated confirming the validity of this new technique. Experimental results at both quasi-static (10^?4 s^?1) and dynamic (100 s^?1, 240 s^?1) strain rates showed that the failure strength of this concrete is rate-sensitive.     

Techniques for measuring stress-strain relations at high strain rates

The qualitative dependence of the mechanical behavior of some materials on strain rate is now well known. But the quantitative relation between stress, strain and strain rate has been established for only a few materials and for only a limited range. This relation, the so-called constitutive equation, must be known before plasticity or plastic-wave-propagation theory can be used to predict the stress or strain distribution in parts subjected to impact stresses above the yield strength. In this paper, a brief review of some of the experimental techniques for measuring the stress, strain, strain-rate relationship is given, and some of the difficulties and shortcomings pointed out. Ordinary creep or tensile tests can be used at plastic-strain rates from 10^?8 to about 10^?1/sec. Special quasi-static tests, in which the stress- and strain-measuring devices as well as the specimen geometry and support have been optimized, are capable of giving accurate results to strain rates of about 10²/sec. At higher strain rates, it is shown that wave-propagation effects must be included in the design and analysis of the experiments. Special testing machines for measuring stress, strain and strain-rate relationships in compression, tension and shear at strain rates up to 10⁵/sec are described, and some of the results presented. With this type of testing machine, the analysis of the data requires certain assumptions whose validity depends upon proper design of the equipment. A critical evaluation of the accuracy of these types of tests is presented.     

Shear band analysis and shear moduli calibration

Strain localization is a well known phenomenon, generally associated with plastic deformation and rupture in solids, especially in geomaterials. In this process, deformation is observed to concentrate in narrow zones called shear bands. This phenomenon has been studied extensively in the last 20 years by different researchers, experimentally, theoretically and numerically. A criterion for the onset of localization can be predicted solely on the basis of the constitutive law of the material, using the so-called shear band analysis. This criterion gives the critical orientation, and the critical stress state and strain for a given loading history. An important point, already stressed by Vardoulakis in 1980, is that in particular, out-of-axes shear moduli play a central role in the criterion. These are the moduli involved in the response to a deviatoric stress increment with principal axes oriented at 45° from total stress principal axes. Out-of-axes shear moduli are difficult parameters to calibrate; common tests, with fixed principal stress and strain directions, do not provide any information on these moduli, as long as they remain homogeneous. Still, real civil engineering and environmental problems are definitely not simple axisymmetric triaxial tests; practical modeling involves complex stress paths, and need complex parameters to be calibrated. Only special tests, like compression–torsion on hollow cylinder tests, or even more complex tests can be used for shear moduli calibration. However, shear band initiation in homogeneous, fixed-axes tests does activate out-of-axes shear. Hence, it is natural that shear band analysis makes shear moduli enter into the analysis.Then, a typical inverse analysis approach can be used here: experimental observation of strain localization in triaxial tests can be used together with a proper shear band analysis for the model considered, in order to determine out-of-axes shear moduli.This approach has been used for a stiff marl in the framework of a calibration study on a set of triaxial tests. The steps of the method are presented, and the bifurcation surface in the stress space is exhibited.     

Fibrin network adaptation to cell-generated forces

Fibrin promotes wound healing by serving as provisional extracellular matrix for fibroblasts that realign and degrade fibrin fibers, and sense and respond to surrounding substrate in a mechanical-feedback loop. We aimed to study mechanical adaptation of fibrin networks due to cell-generated forces at the micron-scale. Fibroblasts were elongated-shaped in networks with ≤?2 mg/ml fibrinogen, or cobblestone-shaped with 3 mg/ml fibrinogen at 24 h. At frequencies f?<?10² Hz, G′ of fibroblast-seeded fibrin networks with ≥?1 mg/ml fibrinogen increased compared to that of fibrin networks. At frequencies f?>?10³ Hz, G″ of fibrin networks decreased with increasing concentration following the power-law in frequency with exponents ranging from 0.75?±?0.03 to 0.43?±?0.03 at 3 h, and of fibroblast-seeded fibrin networks with exponents ranging from 0.56?±?0.08 to 0.28?±?0.06. In conclusion, fibroblasts actively contributed to a change in viscoelastic properties of fibrin networks at the micron-scale, suggesting that the cells and fibrin network mechanically interact. This provides better understanding of, e.g., cellular migration in wound healing.

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Effective elastic shear stiffness of a periodic fibrous composite with non-uniform imperfect contact between the matrix and the fibers

In this contribution, effective elastic moduli are obtained by means of the asymptotic homogenization method, for oblique two-phase fibrous periodic composites with non-uniform imperfect contact conditions at the interface. This work is an extension of previous reported results, where only the perfect contact for elastic or piezoelectric composites under imperfect spring model was considered. The constituents of the composites exhibit transversely isotropic properties. A doubly periodic parallelogram array of cylindrical inclusions under longitudinal shear is considered. The behavior of the shear elastic coefficient for different geometry arrays related to the angle of the cell is studied. As validation of the present method, some numerical examples and comparisons with theoretical results verified that the present model is efficient for the analysis of composites with presence of imperfect interface and parallelogram cell. The effect of the non uniform imperfection on the shear effective property is observed. The present method can provide benchmark results for other numerical and approximate methods.     

Micromechanical study of elastic moduli of loose granular materials

In micromechanics of the elastic behaviour of granular materials, the macro-scale continuum elastic moduli are expressed in terms of micro-scale parameters, such as coordination number (the average number of contacts per particle) and interparticle contact stiffnesses in normal and tangential directions. It is well-known that mean-field theory gives inaccurate micromechanical predictions of the elastic moduli, especially for loose systems with low coordination number. Improved predictions of the moduli are obtained here for loose two-dimensional, isotropic assemblies. This is achieved by determining approximate displacement and rotation fields from the force and moment equilibrium conditions for small sub-assemblies of various sizes. It is assumed that the outer particles of these sub-assemblies move according to the mean field. From the particle displacement and rotation fields thus obtained, approximate elastic moduli are determined. The resulting predictions are compared with the true moduli, as determined from the discrete element method simulations for low coordination numbers and for various values of the tangential stiffness (at fixed value of the normal stiffness). Using this approach, accurate predictions of the moduli are obtained, especially when larger sub-assemblies are considered. As a step towards an analytical formulation of the present approach, it is investigated whether it is possible to replace the local contact stiffness matrices by a suitable average stiffness matrix. It is found that this generally leads to a deterioration of the accuracy of the predictions. Many micromechanical studies predict that the macroscopic bulk modulus is hardly influenced by the value of the tangential stiffness. It is shown here from the discrete element method simulations of hydrostatic compression that for loose systems, the bulk modulus strongly depends on the stiffness ratio for small stiffness ratios.