共查询到17条相似文献,搜索用时 93 毫秒
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荧光可逆调控研究CdTe量子点-吖啶橙-小牛胸腺DNA的相互作用及分析应用 总被引:1,自引:0,他引:1
水相合成了谷胱甘肽(GSH)修饰的CdTe 量子点(QDs). 在PH=7.4的Tris-HCl缓冲溶液中, 吖啶橙(AO)通过静电引力吸附到GSH-CdTe QDs 的表面, 与GSH-CdTe QDs形成了基态复合物, 导致GSH-CdTe QDs的荧光猝灭. 在GSH-CdTe QDs-AO体系中加入小牛胸腺DNA (ctDNA), ctDNA诱导AO从GSH-CdTe QDs表面脱落嵌入其双螺旋结构中, 导致GSH-CdTe QDs的荧光恢复. 根据GSH-CdTe QDs荧光的猝灭和恢复, 实现了量子点荧光的可逆调控. ctDNA引起GSH-CdTe QDs-AO体系荧光恢复强度与ctDNA浓度成良好的线性关系, 检出限为0.13 ng•mL-1, 据此提出了简便快捷、准确、高灵敏测定ctDNA的新方法. 还结合共振瑞利散射(RRS)光谱、吸收光谱和原子力显微镜照片研究了GSH-CdTe QDs-AO-ctDNA三者之间的相互作用, 对相互作用机理进行了讨论并提出了相应的作用模型. 相似文献
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采用共沉淀法制备了掺杂过渡金属元素(Fe,Mn,Cu)的铈锆复合氧化物。利用XRD研究了不同掺杂量的高温稳定性,用BET法测定了样品的比表面积,用TPR研究了掺杂后复合氧化物的还原性能。结果表明,Mn12%的掺杂即使在1000℃也能稳定存在,而Fe和Cu在该温度下均容易析出。同时掺杂Fe,Mn或Fe,Cu两种元素起始还原温度分别为140和100℃,而在宽泛的温度范围内显示了还原活性,这是两种元素还原峰重叠的结果。 相似文献
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光谱法研究盐酸小檗碱与DNA的相互作用 总被引:3,自引:1,他引:3
采用荧光和紫外(UV)光谱等手段,研究了中药有效成分小檗碱(Ber)与脱氧核糖核酸(DNA)的键合作用。结果发现,小檗碱能插入到DNA双螺旋碱基对之间的空腔中,使小檗碱在eλm=530 nm处较弱的荧光强度显著提高;酸度显著影响其相互之间的作用;随着DNA浓度的增大,Ber的荧光强度增大,显示了很好的光敏性能。偏振、荧光猝灭实验等也进一步表明:Ber与DNA的作用方式主要是嵌插结合;离子强度的大小会影响Ber与DNA之间的作用。在pH=3.0适宜酸度条件下,建立了以Ber为探针定量测定DNA的分析方法。方法线性范围为0~5.2×10-5mol/L,精密度(RSD)为2.7%(n=7),检出限为1.43×10-7mol/L。 相似文献
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采用荧光光谱及紫外-可见吸收光谱研究了不同条件下磁性纳米氧化铁(MION)与CdTe量子点的相互作用, 发现MION对CdTe量子点荧光有猝灭作用. 由Stern-Volmer方程分析得到MION与CdTe量子点结合反应的荧光猝灭速率常数Kq值为7.68×1015 mol•L-1•s-1, 结合紫外-可见吸收光谱进一步证实此过程为静态猝灭过程. 并由Lineweaver-Burk方程得到MION与CdTe量子点结合的热力学焓变(?H?)值为21.6 kJ•mol-1、熵变(?S?)值为210.3 J• mol-1•K-1和自由能变(?G?)值为-41.1 kJ•mol-1 (298 K). 对其相互作用机理进行探讨, 结果表明MION对CdTe量子点作用为自发过程, 主要存在静电作用. 相似文献
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炭-/石墨烯量子点作为新兴的炭纳米材料,因具有独特的小尺寸效应和丰富的边缘活性位点而在高性能超级电容器电极材料的研发方面展现出巨大潜力。针对目前炭-/石墨烯量子点在超级电容器电极材料方面的应用优势和存在的关键问题,本文以炭-/石墨烯量子点、量子点/导电炭复合材料、量子点/金属氧化物复合材料、量子点/导电聚合物复合材料以及量子点衍生炭这些电极材料为脉络,梳理了近年来该领域的发展状况,尝试阐释炭-/石墨烯量子点在电极材料、复合材料和衍生炭电极材料中所起到的关键作用,最后对炭-/石墨烯量子点电极材料的发展进行了展望。本综述以期为炭-/石墨烯量子点基电极材料的研究提供一定参考和依据。 相似文献
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Ya-ping Liu Yu-biao Li Prof. Da-jian Huang Prof. Hu Zhang Prof. Ke Chu 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(51):11933-11939
Electrochemical reduction of N2 to NH3 is a promising method for artificial N2 fixation, but it requires efficient and robust electrocatalysts to boost the N2 reduction reaction (NRR). Herein, a combination of experimental measurements and theoretical calculations revealed that a hybrid material in which ZnO quantum dots (QDs) are supported on reduced graphene oxide (ZnO/RGO) is a highly active and stable catalyst for NRR under ambient conditions. Experimentally, ZnO/RGO was confirmed to favor N2 adsorption due to the largely exposed active sites of ultrafine ZnO QDs. DFT calculations disclosed that the electronic coupling of ZnO with RGO resulted in a considerably reduced activation-energy barrier for stabilization of *N2H, which is the rate-limiting step of the NRR. Consequently, ZnO/RGO delivered an NH3 yield of 17.7 μg h−1 mg−1 and a Faradaic efficiency of 6.4 % in 0.1 m Na2SO4 at −0.65 V (vs. RHE), which compare favorably to those of most of the reported NRR catalysts and thus demonstrate the feasibility of ZnO/RGO for electrocatalytic N2 fixation. 相似文献
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Yuichiro Uemura Kairi Yamato Prof. Dr. Ryo Sekiya Prof. Dr. Takeharu Haino 《Angewandte Chemie (International ed. in English)》2018,57(18):4960-4964
Graphene quantum dot (GQD)–organic hybrid compounds (GQD‐ 2 b – e ) were prepared by introducing 3,4,5‐tri(hexadecyloxy)benzyl groups (C16) and linear chains terminated with a 2‐ureido‐4‐[1H]‐pyrimidinone (UPy) moiety onto the periphery of GQD‐ 1 . GQD‐ 2 b – e formed supramolecular assemblies through hydrogen bonding between the UPy units. GPC analysis showed that GQDs with high loadings of the UPy group formed larger assemblies, and this trend was confirmed by DOSY and viscosity measurements. AFM images showed the polymeric network structures of GQD‐ 2 e on mica with flat structures (ca. 1.1 nm in height), but no such structures were observed in GQD‐ 2 a , which only carries the C16 group. GQD‐ 2 c and GQD‐ 2 d formed organogels in n‐decanol, and the gelation properties can be altered by replacing the alkyl chains in the UPy group with ethylene glycol chains (GQD‐ 3 ). GQD can thus be used as a platform for supramolecular polymers and organogelators by suitable chemical functionalization. 相似文献
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Ziyi Liu Fei Li Yi Luo Ming Li Guanghui Hu Xianjuan Pu Tao Tang Jianfeng Wen Xinyu Li Weitao Li 《Molecules (Basel, Switzerland)》2021,26(13)
High-photoluminescence (PL) graphene quantum dots (GQDs) were synthesized by a simple one-pot hydrothermal process, then separated by dialysis bags of different molecular weights. Four separated GQDs of varying sizes were obtained and displayed different PL intensities. With the decreasing size of separated GQDs, the intensity of the emission peak becomes much stronger. Finally, the GQDs of the smallest size revealed the most energetic PL intensity in four separated GQDs. The PL energy of all the separated GQDs shifted slightly, supported by density functional theory calculations. 相似文献
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Prof. Dr. Ryo Sekiya Yuichiro Uemura Prof. Dr. Hiroyoshi Naito Prof. Dr. Kensuke Naka Prof. Dr. Takeharu Haino 《Chemistry (Weinheim an der Bergstrasse, Germany)》2016,22(24):8198-8206
Chemical modification of graphene quantum dots (GQDs) can influence their physical and chemical properties; hence, the investigation of the effect of organic functional groups on GQDs is of importance for developing GQD–organic hybrid materials. Three peripherally functionalised GQDs having a third‐generation dendritic wedge (GQD‐ 2 ), long alkyl chains (GQD‐ 3 ) and a polyhedral oligomeric silsesquioxane group (GQD‐ 4 ) were prepared by the CuI‐catalysed Huisgen cycloaddition reaction of GQD‐ 1 with organic azides. Cyclic voltammetry indicated that reduction occurred on the surfaces of GQD‐ 1 – 4 and on the five‐membered imide rings at the periphery, and this suggested that the functional groups distort the periphery by steric interactions between neighbouring functional groups. The HOMO–LUMO bandgaps of GQD‐ 1 – 4 were estimated to be approximately 2 eV, and their low‐lying LUMO levels (<?3.9 eV) were lower than that of phenyl‐C61‐butyric acid methyl ester, an n‐type organic semiconductor. The solubility of GQD‐ 1 – 4 in organic solvents depends on the functional groups present. The functional groups likely cover the surfaces and periphery of the GQDs, and thus increase their affinity for solvent and avoid precipitation. Similar to GQD‐ 2 , both GQD‐ 3 and GQD‐ 4 emitted white light upon excitation at 360 nm. Size‐exclusion chromatography demonstrated that white‐light emission originates from the coexistence of differently sized GQDs that have different photoluminescence emission wavelengths. 相似文献
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Compared with natural enzymes, nanozymes have the advantages of good catalytic performance, high stability, low cost, and can be used under extreme conditions. Preparation of highly active nanozymes through simple methods and their application in bioanalysis is highly desirable. In this work, a nanozyme based on dispersion of hemin by graphene quantum dot (GQD) is demonstrated, which enables colorimetric detection of glutathione (GSH). GQD was prepared by a one-step hydrothermal synthesis method. Hemin, the catalytic center of heme protein but with low solubility and easy aggregation that limits its catalytic activity, can be dispersed with GQD by simple sonication. The as-prepared Hemin/GQD nanocomplex had excellent peroxidase-like activity and can be applied as a nanozyme. In comparison with natural horseradish peroxidase (HRP), Hemin/GQD nanozyme exhibited a clearly reduced Michaelis–Menten constant (Km) when tetramethylbenzidine (TMB) was used as the substrate. With H2O2 being the substrate, Hemin/GQD nanozyme exhibited a higher maximum reaction rate (Vmax) than HRP. The mechanisms underlying the nanozyme activity were investigated through a free radical trapping experiment. A colorimetric platform capable of sensitive detection of GSH was developed as the proof-of-concept demonstration. The linear detection range was from 1 μM to 50 μM with a low limit of detection of 200 nM (S/N = 3). Determination of GSH in serum samples was also achieved. 相似文献