Metalloantibiotics: synthesis and antibacterial activity of ceftazidime metal complexes

Ceftazidime (Hceftaz) interacts with transition metal(II) ions to give octahedral [M(ceftaz)(H₂O)Cl] complexes [M = Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Cd(II)] which were characterized by physicochemical and spectroscopic methods. The spectra indicated that the ligand is a multidentate chelating agent. The complexes are insoluble in water and common organic solvents and probably have polymeric structures. The antibacterial activity of the metal complexes was found to be lower than that of free ceftazidime.     

Synthesis,characterization and antimicrobial studies of novel ONO donor hydrazone Schiff base complexes with some divalent metal (II) ions

Metal complexes of two general formulae [M(L)(Cl)(H₂O)₂] [M = Mn(II), Co(II), Ni(II) and Cu(II)] and [M(L)(H₂O)] [M = Zn(II) and Cd(II)] with pyrazine-2-carbohydrazone of 2-hydroxy-5-methylacetophenone (H₂L) are synthesized and characterized by microanalytical, thermal, magnetic susceptibility measurement, spectroscopic (IR, ¹H NMR, ¹³C NMR), mass, molar conductance, X-ray powder diffraction, ESR and SEM studies. While the molar conductance measurements in DMSO indicated their non-electrolytic nature, the spectroscopic studies confirmed a tridentate ONO donor behaviour of the ligand towards the central metal ion. Based on the physico-chemical studies monomeric octahedral geometry around Mn(II), Co(II), Ni(II) and Cu(II) ions (i.e. for the first series of complexes) whereas tetrahedral to Zn(II) and Cd(II) ions (i.e. for the second series of complexes) are suggested. Based on the thermal behavior of the complexes, various kinetic and thermodynamic parameters were evaluated using Coats-Redfern method. The ligand and its metal complexes were screened for in vitro antibacterial and antifungal activity against Gram +ve S. aureus, B. subtilis and Gram –ve E. coli and S. typhi. and fungal strains, C. albicans and A. niger. The observed data infer promising biological activity of some of these complexes compared the parent ligand against all bacterial and fungal species.     

Spectral,thermal and magnetic investigations of Mn(II), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) 4-methylphthalates

Conditions for the preparation of Mn(II), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) 4-methylphthalates were investigated and their composition, solubility in water at 295 K and magnetic moments were determined. IR spectra and powder diffraction patterns of the complexes prepared with molar ratio of metal to organic ligand of 1.0:1.0 and general formula: M [ CH₃C₆H₃(CO₂)₂]·nH₂o (n=1-3) were recorded and their decomposition in air were studied. During heating the hydrated complexes are dehydrated in one (Mn, Co, Ni, Zn, Cd) or two steps (Cu) and next the anhydrous complexes decompose to oxides directly (Cu, Zn), with intermediate formation of carbonates (Mn, Cd), oxocarbonates (Ni) or carbonate and free metal (Co). The carboxylate groups in the complexes studied are mono- and bidentate (Co, Ni), bidentate chelating and bridging (Zn) or bidentate chelating (Mn, Cu, Cd). The magnetic moments for paramagnetic complexes of Mn(II), Co(II), Ni(II) and Cu(II) attain values 5.92, 5.05, 3.36 and 1.96 M.B., respectively. This revised version was published online in July 2006 with corrections to the Cover Date.     

Mononuclear transition and non-transition complexes of amlodipine besylate as antihypertensive agent: synthesis,spectral, thermal,and antimicrobial studies

Mononuclear Mn(II), Co(II), Ni(II), Zn(II), Cd(II), Mg(II), Sr(II), Ba(II), Ca(II), Pt(IV), Au(III), and Pd(II) complexes of the drug amlodipine besylate (HL) have been synthesized and characterized by elemental analysis, spectroscopic technique (IR, UV–Vis, solid reflectance, scanning electron microscopy, X-ray powder diffraction, and ¹H-NMR) and magnetic measurements. The elemental analyses of the complexes are confirmed by the stoichiometry of the types [M(HL)(X)₂(H₂O)]·nH₂O [M = Mn(II), Co(II), Zn(II), Ni(II), Mg(II), Sr(II), Ba(II), and Ca(II); X = Cl^? or NO₃ ^?], [Cd(HL)(H₂O)]Cl₂, [Pd(HL)₂]Cl₂, [Pt(L)₂]Cl₂, and [Au(L)₂]Cl, respectively. Infrared data revealed that the amlodipine besylate drug ligand chelated as monobasic tridentate through NH₂, oxygen (ether), and OH of besylate groups in Mn(II), Co(II), Ni(II), Zn(II), Cd(II), Mg(II), Sr(II), Ba(II), Ca(II), and Au(III) complexes, but in Pt(IV) and Pd(II) complexes, the amlodipine besylate coordinates via NH₂ and OH (besylate) groups. An octahedral geometry is proposed for all complexes except for the Cd(II), Pt(IV), and Pd(II) complexes. The amlodipine besylate free ligand and the transition and non-transition complexes showed antibacterial activity towards some Gram-positive and Gram-negative bacteria and the fungi (Aspergillus flavus and Candida albicans).     

Structural studies of metal complexes containing amide ligands

Complexes of Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Pd(II) with di-N-phenyl pyromellitic diimide (PhPMDI) and di-N-pyridyl pyromellitic diimide (PyPMDI) were prepared and characterized based on analytical, molar conductance, magnetic, IR, PMR, electronic and ESR data. Based on analytical and molar conductance, the complexes have been formulated as [M(PhPMDA)(H₂O)₂]_n (M = Mn, Fe, Co, Ni), [Cu(PhPMDA)]_n [Pd₂(PhPMDA)Cl₂(H₂O)₂], [M(PyPMDA)]_n (M = Mn, Fe, Co, Ni and Cu) and [Pd₂(PyPMDA)Cl₂] In all these complexes PhPMDA acts as a mononegative bidentate ligand whereas PyPMDA acts as a mononegative tridentate one in the form of amide rather than imide. The geometries of the complexes have been proposed based on the electronic spectra. The various bonding parameters have been calculated from the ESR spectra of Cu(II) complexes.     

Polymer complexes. LXXI. Spectroscopic studies,thermal properties,DNA binding and antimicrobial activity of polymer complexes

Polymer complexes of Co(II), Ni(II), Mn(II), Cr(III) and Cd(II) were prepared by the reaction of 3‐allyl‐5‐[(4‐nitrophenylazo)]‐2‐thioxothiazolidine‐4‐one (HL) with metal ions. The structure of polymer complexes was characterized by elemental analysis, IR, UV–Vis spectra, X‐ray diffraction analysis, magnetic susceptibility, conductivity measurements and thermal analysis. Reaction of HL with Co(II), Ni(II), Mn(II), Cr(III) and Cd(II) ions (acetate or chloride) give polymer complexes ( 1–5 ) with general stoichiometric [M(L)(O₂CCH₃)(H₂O)₂]_n (where L = anionic of HL and M = Co(II) (1) or Ni(II) (2) ), [Mn(HL)₂(OCOCH₃)₂]_n (3) , [Cr(L)₂(Cl)(H₂O)]_n (4) and [Cd(HL)(O₂CCH₃)₂]_n (5) . The value of HOMO–LUMO energy gap (ΔE) for forms (A‐C) of monomer (HL) is 2.529, 2.296 and 2.235 eV, respectively. According to ΔE value, compound has minimum ΔE is the more stable, so keto hydrazone form (C) is more stable than the other forms (azo keto form (A), azo enol form (B)). The interaction between HL, polymer complexes of Co(II), Ni(II), Mn(II), Cr(III) and Cd(II) with Calf thymus DNA showed hypochromism effect. The HL and its polymer complexes were tested against some bacterial and fungal species. The results showed that the Cr(III) polymer complex (4) has more antibacterial activity than HL and polymer complexes (1–3 and 5) against Bacillus subtilis, Staphylococcus aureus and Salmonella typhimurium.     

Complexation of 2,3-dihydroxyquinaline with some bivalent <Emphasis Type="Italic">d</Emphasis> metals. Crystal and molecular structures of 2,3-dihydroxyquinoline

The reactions of Mn(II), Fe(II), Co(II), Ni(II), Cu(II), and Cd with 2,3-dihydroxyquinoline (H₂L) were used to synthesize and identify the complexes M(HL)₂ · 2H₂O (M = Mn, Ni, Cu), Fe(HL)OH · 2H₂O, Co(HL)OH · H₂O, Cd(H₂L)Cl₂, where H₂L participates in the coordination in the monoanionic or neutral forms with the formation of the chelate cycles. Single crystal was isolated and the crystal structure of H₂L was determined. The spectral characteristics of a neutral and anionic form of the ligand were measured, and its complexation with MCl₂ was studied in the ethanol solutions. The acidity constant of H₂L and the formation constants of the complexes in solutions were calculated.     

Spectral, magnetic and biological studies of 1,4-dibenzoyl-3-thiosemicarbazide complexes with some first row transition metal ions

The ligand 1,4-dibenzoyl-3-thiosemicarbazide (DBtsc) forms complexes [M(DBtsc-H)(SCN)] [M = Mn(II), Co(II) or Zn(II)], [M(DBtsc-H) (SCN)(H₂O)] [M = Ni(II) or Cu(II)], [M(DBtsc-H)Cl] [M = Co(II), Ni(II), Cu(II) or Zn(II)] and [Mn(DBtsc)Cl₂], which have been characterized by elemental analyses, magnetic susceptibility measurements, UV/Vis, IR,¹H and¹³C NMR and FAB mass spectral data. Room temperature ESR spectra of the Mn(II) and Cu(II) complexes yield <g> values, characteristic of tetrahedral and square planar complexes respectively. DBtsc and its soluble complexes have been screened against several bacteria, fungi and tumour cell lines.     

Coordination of carbonyl oxygen in the complexes of polymeric <Emphasis Type="Italic">N</Emphasis>-crotonyl-2-hydroxyphenylazomethine

The Schiff base N-crotonyl-2-hydroxyphenylazomethine HL, derived from the reaction of acrylamide and salicylaldehyde, was synthesised. Polymeric complexes were obtained from the reaction of polymeric HL with divalent metals. The mode of bonding and overall geometry of the complexes were determined through physico-chemical and spectroscopic methods and compared with that previously reported for the analogous monomeric ligand. These studies revealed tetrahedral geometries around the metal centres for Mn(II), Co(II), Zn(II), Cd(II) and Hg(II) complexes of general formula [M(L)Cl], octahedral for Ni(II) and Cu(II) complexes of general formula [M′(L)Cl(H₂O)₂], and square planar for Pd(II) complex of general formula [Pd(L)Cl].     

Homo‐ and heteropolynuclear copper(II) complexes containing a new diimine–dioxime ligand and 1,10‐phenanthroline: synthesis,characterization, solvent‐extraction studies,catalase‐like functions and DNA cleavage abilities

A series of homo‐, heterodinuclear and homotrinuclear copper(II) complexes containing a new Schiff base ligand and 1,10‐phenanthroline were synthesized. Based on results of elemental analyses, FTIR, ¹H‐ and ¹³C‐NMR spectra, conductivity measurements and magnetic susceptibility measurements, the complexes had general compositions {[Cu(L)(H₂O)M(phen)₂](ClO₄)₂ [M = Cu(II), Mn(II), Co(II)]} and {[Cu₃(L)₂(H₂O)₂](ClO₄)₂}. The metal:L:phen ratio is 2:1:2 for the dinuclear copper(II) complexes and the metal:L ratio was 3:2 for the trinuclear copper(II) complex. The liquid–liquid extraction of various transition metal cations [Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Pb(II), Cd(II), Hg(II)] from the aqueous phase to the organic phase was carried out using the diimine–dioxime ligand. It was concluded that the ligand can effectively be used in solvent extraction of copper(II) from the aqueous phase to the organic phase. Furthermore, catalytic activitiy of the complexes for the disproportionation of hydrogen peroxide was also investigated in the presence of imidazole. Dinuclear copper(II)–manganese(II) complex has some similarity to manganese catalase in structure and activity. The interaction between these complexes and DNA has also been investigated by agarose gel electrophoresis; we found that the homo‐ and heterodinuclear copper complexes can cleave supercoiled pBR322 DNA to nicked and linear forms in the presence of H₂O₂. Copyright © 2009 John Wiley & Sons, Ltd.     

Synthesis and antibacterial activity of cephalexin metal complexes

The interactions of cephalexin (Hcepha) with transition and d¹⁰ metal ions have been investigated. The complexes [M(cepha)Cl]nH₂O [M?=?Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II), Hg(II)] were characterized by physicochemical and spectroscopic methods. The IR and ¹H NMR spectra of the complexes suggest that cephalexin behaves as a monoanionic tridentate ligand. In vitro antibacterial activities of Hcepha and the complexes were tested.     

Investigation of mononuclear,dinuclear, and trinuclear transition metal (II) complexes derived from an asymmetric Salamo‐based ligand possessing three different coordination modes

A new asymmetric Salamo‐based ligand H₂L was synthesized using 3‐tert‐butyl‐salicylaldehyde and 6‐methoxy‐2‐[O‐(1‐ethyloxyamide)]‐oxime‐1‐phenol. By adjusting the ratio of the ligand H₂L and Cu (II), Co (II), and Ni (II) ions, mononuclear, dinuclear, and trinuclear transition metal (II) complexes, [Cu(L)], [{Co(L)}₂], and [{Ni(L)(CH₃COO)(CH₃CH₂OH)}₂Ni] with the ligand H₂L possessing completely different coordination modes were obtained, respectively. The optical spectra of ligand H₂L and its Cu (II), Co (II) and Ni (II) complexes were investigated. The Cu (II) complex is a mononuclear structure, and the Cu (II) atom is tetracoordinated to form a planar quadrilateral structure. The Co (II) complex is dinuclear, and the two Co (II) atoms are pentacoordinated and have coordination geometries of distorted triangular bipyramid. The Ni (II) complex is a trinuclear structure, and the terminal and central Ni (II) atoms are all hexacoordinated, forming distorted octahedral geometries. Furthermore, optical properties including UV–Vis, IR, and fluorescence of the Cu (II), Co (II), and Ni (II) complexes were investigated. Finally, the antibacterial activities of the Cu (II), Co (II), and Ni (II) complexes were explored. According to the experimental results, the inhibitory effect was found to be enhanced with increasing concentrations of the Cu (II), Co (II), and Ni (II) complexes.     

Metal chelates of salicyl-4-aminoantipyrine

The formation constants of salicyl-4-amino-2,3-dimethyl-1-phenyl-3-pyrazoline-5-one (SAAP) complexes with 3d transition metal ions [Cu(II), Ni(II), Co(II), Zn(II) and Mn(II)] have been determined in 60% ethanol-water medium ofμ = 0.1M (NaCl) at 25°C. It is observed that the formation constants for chelates with 3d transition metals follow the order Mn(II) < Co(II) < Ni(II) < Zn(II) < Cu(II). The effects of metal ions, ionic radii, electronegativities and ionization potentials on chelate formation constants are discussed. Complexes of UO₂(II) and Pd(II) have been synthesized and characterised by elemental analysis, electrolytic conductance, IR spectra and magnetic measurements. The ligand forms the complexes PdLCl and UO₂L₂,2H₂O, where L is a uninegatively charged tridentate ligand (ONO donor sets).     

Photoacoustic spectra of o-sulphobenzoimide transition metal complexes

The photoacoustic spectra of o-sulphobenzoimide transition metal complexes with a general formula [M(C₇H₄NO₃S)₂(H₂O)₄]·.2H₂O, [M = Mn(II), Fe(II), Co(II), Ni(II), Cu(II)] have been determined in the 300–800 nm region at room temperature. The spectra have been interpreted qualitatively by the ligand field theory. All absorption bands have been assigned.     

Mn(II), Co(II), Ni(II) and Cu(II) complexes of a tertraaza macrocyclic ligand: Synthesis, characterization and biological screening

Manganese(II), cobalt(II), nickel(II) and copper(II) complexes with 1,5,11,15-tetraaza-21,22-dioxo-tricyclo [19,3,1,I^6,10]-5,10,15-20-dicosatetraene (L), as a new macrocyclicligand, have been synthesized with and characterized by elemental analysis, molar conductance measurements, magnetic susceptibility measurements, mass, IR, electronic and EPR spectral studies. The molar conductance measurements of the complexes in DMF correspond to non-electrolytic nature of Mn(II), Co(II) and Cu(II) complexes, while showing a 1:2 electrolyte for thew Ni(II) complexe. Thus, these complexes may be formulated as [M(L)X₂] and [Ni(L)]X₂ (where M = Mn(II), Co(II) and Cu(II) and X = Cl^- and NO₃ ^-). On the basis of IR, electronic and EPR spectral studies, an octahedral geometry has been assigned for Mn(II) and Co(II), a square planar for Ni(II) and tetragonal for Cu(II) complexes. In vitro ligand and its metal complexes were also screened against the growth of some fungal and bacterial species in order to assess their antimicrobial properties.     

Metal complexes of tetradentate azo-dye ligand derived from 4,4′-oxydianiline: Preparation,structural investigation,biological evaluation and MOE studies

The Cr (III), Mn (II), Fe (III), Co (II), Ni (II), Cu (II) and Cd (II) complexes were prepared by reaction of its metal chlorides with new azo-dye ligand (H₂L). The ligand derived from 4,4′-oxydianiline and 2-amino-4-chlorophenol was synthesized in a 1:2 molar ratio. The structure of the ligand and its metal complexes was investigated using different tools such as elemental analysis (C, H, N and M), molar conductivity, IR, UV–vis, ¹H-NMR, mass spectrometry and thermogravimetric and differential thermogravimetric studies. The data showed that the ligand acted as a N,N,O,O-binegative tetradentate ligand. All metal complexes had a octahedral structure as depicted by spectral and elemental analyses. The conductivity data showed the electrolytic nature of the Cr (III) and Fe (III) complexes while the other complexes were nonelectrolytes. Thermal analysis studies showed the decomposition of the complexes in four to five steps with the weight loss of hydrated water in the first decomposition step followed by the coordinated water and ligand molecules. Biological activity was tested for the prepared compounds against four bacterial species (Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and against two fungal species (Aspergillus fumigatus and Candida albicans). Also, all complexes were screened for anticancer activities against a breast cancer (MCF-7) cell line. The [Co(L)(H₂O)₂] complex showed the lowest IC₅₀ value. Molecular docking is a key tool in computer drug design. Therefore, investigation of protein receptors and ligand interaction plays a vital role in the design of structurally based drugs. As a result, docking studies were investigated for H₂L ligand, [Mn(L)(H₂O)₂] and [Ni(L)(H₂O)₂] complexes with 5KBC, 3V7B and 4G9M receptors.     

Synthesis and genotoxicity of Schiff base transition metal complexes

A new ONNO‐type azomethine ligand, 2,2′‐(ethane‐1,2‐diylidenedinitrilo)dibenzoic acid, (YLH₂) ( 1 ) has been prepared by the condensation of 2‐aminobenzoic acid and glyoxal. The coordination compounds [Ni(YL)] ( 2 ), [Co(YL)] ( 3 ), [Cu(YL)(H₂O)] ( 4 ), [Zn(YL)] ( 5 ), and [Cd(YL)] ( 6 ) of the YLH₂ ligand with five transition metal ions, Ni(II) Co(II), Cu(II), Zn(II), and Cd(II) have been prepared. The structures of these new azomethine compounds are proposed on the basis of the elemental analyses, proton nuclear magnetic resonance, infrared, ultraviolet–visible spectroscopy, and X‐ray powder diffraction patterns. Elemental analyses indicate a ligand metal ratio of 1:1 in the coordination compounds. X‐ray powder diffraction parameters for [Cu(YL)(H₂O)] and [Cd(YL)] compounds correspond to orthorhombic and monoclinic structures, respectively. The ligand acts as a tetradentate ligand bending through oxygen atoms of the hydroxyl groups of benzoic acid and nitrogen atoms of the azomethine groups. In addition, the ligand and its metal complexes have been studied for their possible genotoxic potential. © 2011 Wiley Periodicals, Inc. Heteroatom Chem 22:119–130, 2011; View this article online at wileyonlinelibrary.com . DOI 10.1002/hc.20665     

Physicochemical and Biological Characterization of Transition Metal Complexes with a Nitrogen Donor Tetra-dentate Novel Macrocyclic Ligand

A novel tetradentate nitrogen donor [N₄] macrocyclic ligand, i.e. 3,5,13,15,21,22-hexaaza-2,6,12,16-tetramethyl-4,14-dithia-tricyclo[15.3.1.1(7–11)]docosane-1(21),2,5,7,9,11(22),12,15,17,19-decaene, has been synthesized. Mn(II), Co(II), Ni(II) and Cu(II) complexes with this ligand have been prepared and subjected to elemental analyses, molar conductance measurements, magnetic susceptibility measurements, mass, ¹H-n.m.r. (Ligand), i.r., electronic, and e.p.r. spectral studies. On the basis of molar conductance the complexes may be formulated as [M(L)X₂] and [Ni(L)]X₂ [where M = Mn(II), Co(II) and Cu(II), and X = Cl⁻ and NO₃⁻] due to their nonelectrolytic nature in dimethylsulphoxide (DMSO). All the complexes are of the high spin type and are six coordinated. On the basis of i.r., electronic and e.p.r. spectral studies an octahedral geometry has been assigned to Mn(II) and Co(II), square planar for Ni(II) complexes, and tetragonal for Cu(II) complexes. The antimicrobial activities of the ligand and its complexes, as growth inhibiting agents, have been screened in vitro against several species of bacteria and plant pathogenic fungi.     

Mass, EPR, IR and electronic spectroscopic studies on newly synthesized macrocyclic ligand and its transition metal complexes

Manganese(II), cobalt(II), nickel(II) and copper(II) complexes have been synthesized with a new tetradentate ligand viz. 1,3,7,9-tetraaza-2,4,8,10-tetraketo-6,12-diphenyl-cyclododecane (L) and characterized by the elemental analysis, molar conductance measurements, magnetic susceptibility measurements, mass, ¹H NMR, IR, electronic and EPR spectral studies. The molar conductance measurements of the complexes in DMF correspond to be nonelectrolytic nature for Mn(II), Co(II) and Cu(II) while 1:2 electrolytes for Ni(II) complexes. Thus, these complexes may be formulated as [M(L)X₂] and [Ni(L)]X₂ (where M = Mn(II), Co(II) and Cu(II) and X = Cl⁻ and NO₃⁻).On the basis of IR, electronic and EPR spectral studies an octahedral geometry has been assigned for Mn(II) and Co(II) complexes, square-planar for Ni(II) whereas tetragonal for Cu(II) complexes. The ligand and its complexes were also evaluated against the growth of bacteria and pathogenic fungi in vitro.     

Physicochemical characterization of nanobidentate ferrocene‐based Schiff base ligand and its coordination complexes: Antimicrobial,anticancer, density functional theory,and molecular operating environment studies

A novel bidentate Schiff base ligand (HL, Nanobidentate Ferrocene based Schiff base ligand L (has one replaceable proton H)) was prepared via the condensation of 2‐amino phenol with 2‐acetyl ferrocene. The ligand was characterized using elemental analysis, mass spectrometry, infrared (IR) spectroscopy, ¹proton nuclear magnetic resonance (H‐NMR) spectroscopy, scanning electron microscopy (SEM), and thermal analysis. The corresponding 1:1 metal complexes with some transition‐metal ions were additionally characterized by their elemental analysis, molar conductance, SEM, and thermogravimetric ana1ysis (TGA). The complexes had the general formula [M(L)(Cl)(H₂O)₃]xCl·nH₂O (M = Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II), and Cd(II)), (x = 0 for Mn(II), Co(II), Ni(II), Cu(II), Zn(II), and Cd(II), x = 1 for Cr(III) and Fe(III)), (n = 1 for Cr(III), n = 3 for Mn(II) and Co(II), n = 4 for Fe(III), Ni(II), Cu(II), Zn(II), and Cd(II)). Density functional theory calculations on the HL ligand were also carried out in order to clarify molecular structures by the B31YP exchange‐correlation function. The results were subjected to molecular orbital diagram, highest occupied mo1ecu1ar orbital–lowest occupied molecular orbital, and molecular electrostatic potential calculations. The parent Schiff base and its eight metal complexes were assayed against four bacterial species (two Gram‐negative and two‐Gram positive) and four different antifungal species. The HL ligand was docked using molecular operating environment 2008 with crystal structures of oxidoreductase (1CX2), protein phosphatase of the fungus Candida albicans (5JPE), Gram(?) bacteria Escherichia coli (3T88), Gram(+) bacteria Staphylococcus aureus (3Q8U), and an androgen‐independent receptor of prostate cancer (1GS4). In order to assess cytotoxic nature of the prepared HL ligand and its complexes, the compounds were screened against the Michigan cancer foundation (MCF)‐7 breast cancer cell line, and the IC₅₀ values of compounds were calculated.