Phospholipids ofGoebelia seeds

The qualitative and quantitative compositions of the phospholipids of the seeds ofGoebelia pachycarpa and the fatty-acid composition of the total phospholipids and of homogeneous fractions have been studied. The molecular weights of the main fractions have been calculated.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 615–618, September–October, 1979.     

Phospholipids of the cotton plant of variety Tashkent-3. II. Structure of the main phospholipid fractions

Summary 1. Individual fractions of phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, and the X₂ and X₃ phospholipids have been obtained from the combined phospholipids, freed from carbohydrates, of the seed kernels of the cotton plant of variety Tashkent-3.2. The compositions and position distributions of the fatty acids in the main fractions of phospholipids have been studied.3. The possible molecular composition of the phosphatidylcholines, phosphatidylethanolamines, and phosphatidylinositols have been calculated.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 155–158, March–April, 1976.     

A comparative study of the phospholipids of kenaf seeds

Summary The qualitative and qualitative compositions of the phospholipids of kenaf seeds of the variety Opytnyi-1961 have been investigated. It has been established that the main components of the total phospholipids are phosphatidylcholines, phosphatidylethanolamines, and phosphatidylinositols, and minor components are N-acyl-PEs and their lyso analogs The structural analysis of these homogeneous fractions has been performed, and the molecular compositions of the main fractions have been calculated. On comparing the results obtained with those of other varieties of kenaf investigated previously, some differences have appeared in the qualitative, quantitative, molecular, and fatty-acid compositions of the total phospholipids and of individual fractions of them.The differences found are possibly due to the variety of characteristics of the plant. For the N-acyllyso-PEs the structure of 2-acylglycerophosphoryl-N-acylethanolamines has been established.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 559–562, September–October, 1978.     

Study of the fractional composition of the dioxane lignin from the cotton plant

Summary 1. The dioxane lignin of ripe cotton-plant stems has been separated into six fractions of different molecular weights which were fairly homogeneous and differed considerably in their molecular weights.2. It follows from the semiempirical formulas that in all the fractions guaiacyl structural units predominated. The chemical nonidentity of the fractions is shown by the different amounts of functional groups in the phenylpropane structural units and by the relative optical densities of the main bands in the IR spectra of the fractions.3. The low-molecular-weight fraction differed markedly from the others by a higher content of carbohydrates bound to the lignin and by a greater degree of oxidation.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 69–74, January–February, 1979.     

Phospholipids of the medium-fiber cotton plant variety 159-F

Summary 1. The qualitative and quantitative compositions of the PLs of an industrial medium-fiber cotton plant of variety 159-F (1972 and 1974 harvests) have been considered. It has been shown that the total PL amounted to 1.76 and 1.85% and consisted of 60–65% PC, 16–18% PI, 6–8% PE, and 10–14% of combined minor components, respectively.2. The distribution of the lipid phosphorus has been studied during the isolation of PLs by selective solvents. The lipid phosphorus amounted to 5.5% of the total phosphorus in the seed kernels.3. The qualitative and quantitative compositions of the fatty acids of the individual lipid fractions and of the individual PCs, PIs, and PEs have been determined. The position distribution of the fatty acids of the main groups of PLs has been established and a possible molecular composition has been given.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 198–202, March–April, 1977.     

Investigation of the protein component of the lipopolysaccharide-protein complex ofYersinia pseudotuberculosis

It has been established by electrophoresis in polyacrylamide gel that the protein component of the lipopolysaccharide-protein complex ofYersinia pseudotuberculosis consists of two poly-peptides with molecular weights of 42,000–45,000 and 18,000–20,000. These polypeptides have been detected in the cell wall ofY. pseudotuberculosis as the main components. Protein fractions B-2 and B-3, differing with respect to solubility in phenol, were isolated from the LPPC ofY. pseudotuberculosis by extraction with hot 45% phenol. These protein fractions had the same polypeptide composition, which is characteristic for membrane proteins. B-3 included the LPS component and B-2 contained lipid A or fragments of it as impurity. Fractions B-3 and B-2 precipitated with antiserum to the LPPC.Pacific Ocean Institute of Bioorganic Chemistry of the Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 92–97, January–February, 1980.     

Polysaccharides ofEremurus. X. Characteristics of the polysaccharides ofEremurus lactiflorus andE. luteus

Water-soluble polysaccharides have been isolated from two species ofEremurus — E. lactiflorus andE. luteus — with yields of 13.5% and 20.5%, respectively. They contained mainly glucose and mannose in ratios of 1:5 and 1:3.1. The polysaccharides ofE. lactiflorus were separated from a column of DEAE-cellulose. The yield of neutral fraction was 10.3%. Gel filtration of the polysaccharides on Sephadex G-200 showed their polydispersity. Homogeneous fractions were obtained by fractional precipitation with ethanol. They have been characterized with respect to monosaccharide composition, molecular weight, and IR spectra.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 604–608, September–October, 1979.     

Isolation of individual fractions of dioxane lignin from kenaf stems and their study

Summary 1. Three fractions of dioxane lignin have been isolated from kenaf stems of the variety Uzbekskii-15-74 by stepwise extraction with aqueous dioxane containing HCl.2. On the basis of the results of elementary and functional analyses, semiempirical formulas of all the fractions have been derived, and their IR and UV spectra have been recorded.3. It has been shown that an increase in the time of extraction leads to a rise in the molecular weight of the fractions and to a decrease in their degree of polydispersity.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 637–641, September–October, 1978.     

Phospholipids of kenaf of variety Opytnyi-1847

The total phospholipids of kenaf of variety Opytnyi-1847 and homogeneous fractions of them have been studied. A known law has been confirmed — positions 2 of the main fractions of phospholipids are esterified predominantly with unsaturated fatty acids and in N-aceylated phospholipids the amide-bound acids are more saturated than the O-acyls.Institute of the Chemistry of Plant Substances of the Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 775–777, November–December, 1980.     

Study of the membrane proteins of the cotton plant using monoclonal antibodies

The total membranes from cottonplant shoots have been used as the antigenic material for obtaining monoclonal antibodies (mcABs). Eleven stable hybrids producing mcABs to components of the membrane proteins have been selected. Antibodies to various membrane fractions and to fractions of lectin-like proteins have been revealed. Structural characteristics of the carbohydrate components of cottonplant glycoproteins have been determined by cross-immunochemical analysis.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Fax 627071. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 83–87, January–February, 1994.     

Kinetics of the qualitative and quantitative changes of various protein fractions in the ontogenesis of protoplast and hybrid cultures ofTrichoderma harzianum

The protein composition and the kinetics of the quantitative changes in the various protein fractions during the ontogenesis of protoplast and hybrid cultures ofTrichoderma harzianum have been studied. In the exponential growth phases of both cultures the highest amount of protein was contained in the water-soluble (33.0–33.2%) and salt-soluble (31.0–32.0%) fractions. The protein contents of the water-soluble fractions of the cultures studied were similar to those in meat and clover and were 3.5 times greater than in yeast.Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 41 71 29. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 209–211, March–April, 1998.     

Polysaccharides ofCardaria repens and a study of their IR spectra

Five fractions have been obtained by the fractional precipitation of the water-soluble polysaccharide with alcohol: water-insoluble (fractions 1 and 2) and water-soluble (fractions 3-5). Their Fourier IR spectra have been studied and, on the basis of the results obtained, it is suggested that the water solubility of the fractions examined is connected with strong hydrogen bonds.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14 75. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 202–205, March–April, 1995. Original article submitted November 14, 1994.     

Phospholipids of seeds of kenaf of the variety "Uzbekskii-1503"

Summary The qualitative and quantitative compositions of the combined phospholipids of the seeds of kenaf of the variety "Uzbekskii-1503" have been determined for the first time.The fatty-acid composition of the combined phospholipids and triglycerides and also of the main and two minor components of the phospholipids have been studied. On the basis of the results of acid hydrolysis and of IR spectroscopy, the glycerophospholipid structures of the main and minor components of the total material have been confirmed. The enzymatic hydrolysis of the main fractions of the total material has been performed: the position distribution of the fatty acids has been determined and from this the possible molecular compositions of the phosphatidylcholines, phosphatidy lethanolamines, and phosphatidy linositols have been calculated. Kenaf seeds can serve as a source of the medicinal preparation phytin.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 485–487, July–August, 1977.     

Glycolipids of the woody verdure ofLarix sibirica

The compositions of the glycolipids of the main fractions of the woody verdure of the Siberian larch — needles and shoots — have been studied. The dynamics of the amounts of individual glycolipids in the course of vegetation have been established. The qualitative and quantitativae compositions of the fatty acids of the total glycolipids of the needles and shoots have been determined.Siberian Technological Institute, 660049, Krasnoyarsk, Prospekt Mira, 82. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 183–187, March–April, 1994.     

Phytoglycolipids of the grape

The qualitative and quantitative compositions of the grape and its component parts — flesh, skin, seeds — have been studied. Ten fractions have been identified, the main ones being cerebrosides and monogalactosyldiglycerides. The compositions of the fatty acids, of the sphingosine bases, of the sterols, and of the carbohydrates of the individual fraction of phytoglycolipids have been determined.Physicochemical Institute of the Academy of Sciences of the Ukrainian SSR, Odessa. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 296–300, May–June, 1984.     

An investigation of the fractional composition of dioxane lignin of the cotton plant by the PMR method

Analysis of the PMR spectra of six fractions of the dioxane lignins of the cotton plant differing considerably in molecular weight has confirmed their chemical nonidentity. It has been established that the low-molecular-weight fractions are less condensed than the high-molecular-weight fractions. It has been found that aliphatic OH groups are approximately evenly distributed between the - and -C atoms of the lignin side chain.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 116–120, January–February, 1980.     

A study of the mineral fraction of the polysaccharide complex of the leaves ofPlantago major and Plantaglyutsid

The qualitative and quantitative compositions of the mineral fractions of the polysaccharide complexes of rippleseed plantain leaves and of Plantaglyutsid have been studied.Tashkent Pharmaceutical Institute. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 737–739, November–December, 1985.     

Phosphatase activities of fractions of the venom of Renard's viperVipera ursini renardi

The separation of the venom of Renard's viper by filtration through Sephadex G-75 gel has been performed and phosphatase activities have been determined in the fractions and in the course of separation. The bulk of the activity of the phosphatases investigated was concentrated in the high-molecular-weight fractions I and II. A correlation has been found in the distribution of the DNase and ATP-pyrophosphatase activities with the phosphodiesterase activity in the venom fractions. There is no correlation in the distribution of the RNase and phosphodiesterase activity.Institute of Biochemistry, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 783–786, November–December, 1985.     

Phospholipids of Gossypium barbadense

Summary From the total phospholipids of the cotton plant of the thin-fibered variety 5904-I(Gossypium barbadense) chromatographically homogeneous fractions of phosphatidylethanolamines, phosphatidylcholines, and phosphatidylinositols have been isolated. The well-known glycerophospholipid structure of these fractions has been established on the basis of their IR spectra and the products of their acid hydrolysis.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 558–560, September–October, 1974.     

Kinetic features of the hydrolysis of the hemicelluloses of sunflower seed husks

A kinetic analysis has been made of the hydrolysis reactions of sunflower seed husks. The presence of six kinetically homogeneous fractions in the hemicellulose complex has been shown. The kinetic characteristics and the amounts of each fraction have been determined.St. Petersburg Academy of Wood Technology. Translated from Khimiya Prirodnykh Soedeninii, No. 1, pp. 136–141, January–February, 1993.