Lipoxygenase inhibiting constituents from Indigofera hetrantha

A lignan and two acylphloroglucinols have been isolated from the ethyl acetate soluble fraction of the whole plant of Indigofera hetrantha. Their structures have been assigned on the basis of spectral analysis including 1D and 2D NMR techniques. Compounds 1-3 displayed promising inhibitory potential against enzyme lipoxygenase in concentration-dependent manner.     

Hepatitis B virus inhibiting constituents from Herpetospermum caudigerum

From the ethanol extract of the seeds of Herpetospermum caudigerum wall, one new lignan compound 1, was isolated and characterized along with three known compounds 2, 3 and 4. The structure elucidation of the isolated new compound was performed on the basis of spectroscopic and chemical evidence. The structures of known compounds were determined by comparison of spectral data and physical data with those previously reported. The activity inhibiting hepatitis b virus was evaluated. Preliminary studies showed that compound 1 and 2 displayed promising inhibitory potential against hepatitis b virus.     

Chemical studies on the Philippine crude drug calumbibit (seeds of Caesalpinia bonduc): the isolation of new cassane diterpenes fused with alpha,beta-butenolide

New cassane diterpenes named neocaesalpins C and D were isolated from the Philippine crude drug calumbibit botanically originating from the seeds of Caesalpinia bonduc (Fabaceae), and their structures were elucidated on the basis of the spectroscopic evidence. These compounds are characterized by the presence of the alpha,beta-butenolide moiety. Although a number of cassane furanoditerpenes have been known to occur in the same plant species, such constituents could not be isolated from the crude drug of Philippine origin in this study. It is presumed that the chemical difference resulted from chemical differentiation of the species.     

棉籽化学成分的分析

采用索氏提取法对棉籽中棉籽油进行了提取,以气相色谱-质谱联用仪进行了分析,分离鉴定出10种脂肪酸,占棉籽油总量的99.52%。并对其主要成分进行了鉴定,主要化学成分为:棕榈酸26.68%,亚油酸66.05%。用蒸馏-萃取法提取棉花籽中挥发性物质,测得棉籽挥发油的量为0.80%,用GC/MS法从棉籽挥发油中分离并确定出16种化学成分。用峰面积归一化法通过化学工作站数据处理系统,得出各化学成分在挥发油中的质量分数。其中主要成分为:石竹烯量高达33.27%。     

Analysis of chemical constituents in Cistanche species

Species of the genus of Cistanche (Rou Cong Rong in Chinese) are perennial parasite herbs, and are mainly distributed in arid lands and warm deserts. As a superior tonic for the treatment of kidney deficiency, impotence, female infertility, morbid leucorrhea, profuse metrorrhagia and senile constipation, Cistanche herbs earned the honor of "Ginseng of the desert". Recently, there has been increasing scientific attention on Herba Cistanche for its remarkable bioactivities including antioxidation, neuroprotection, and anti-aging. The chemical constituents of Cistanche plants mainly include volatile oils and non-volatile phenylethanoid glycosides (PhGs), iridoids, lignans, alditols, oligosaccharides and polysaccharides. Pharmacological studies show that PhGs are the main active components for curing kidney deficiency, antioxidation and neuroprotection; galactitol and oligosaccharides are the representatives for the treatment of senile constipation, while polysaccharides are responsible for improving body immunity. In this paper, the advances on the chemical constituents of Cistanche plants and their corresponding analyses are reviewed.     

Anti-plasmodial and cholinesterase inhibiting activities of some constituents of Psorospermum glaberrimum

Glaberianthrone (1), a new bianthrone was isolated from the hexane extract of the stem bark of Psorospermum glaberrimum together with thirteen known compounds: 3-geranyloxyemodin anthrone (2), friedelan-3-one (3), 3-prenyloxyemodin anthrone (4), 3-geranyloxyemodin (5), 3-prenyloxyemodin (6), friedelan-3-ol (7), acetylvismione D (8), betulinic acid (9), 2-geranylemodin (10), bianthrone A2b (11), bianthrone 1a (12), emodin (13) and 2-prenylemodin (14). The structures of the isolated compounds were established by means of spectroscopic methods. The extracts and the isolated compounds were tested in vitro for their anti-plasmodial activity against Plasmodium falciparum (chloroquine resistant strain W2) and for their acetyl- and butyrylcholinesterase inhibitory properties. The n-hexane extract showed good anti-plasmodial activity against P. falciparum W2 strain, with IC(50) of 0.87 microg/ml. It also exhibited 65.5% and 98.2% of acetyl- and butyrylcholinesterase inhibition at 0.2 mg/ml, respectively. Compounds 2 and 8 showed the best potencies against P. falciparum W2 strain with IC(50) of 1.68 microM and 0.12 microM, (0.66 microg/ml and 0.054 microg/ml) respectively. All tested compounds showed good butyrylcholinesterase inhibition activities with compound 12 displaying the best potency (IC(50) 9.25+/-0.25 microM). All the tested compounds showed weak inhibitory activity against acetylcholinesterase.     

On the chemical constituents of Dipsacus asper

Bioassay-guided fractionation of 95% EtOH extract from the roots of Dipsacus asper lead to the isolation of some phenolic acids (caffeic acid, 2,6-dihydroxycinnamic acid, vanillic acid, 2'-O-caffeoyl-D-glucopyranoside ester, and caffeoylquinic acid) as the major active components, and five new iridoid glucoside dimers (1-5) and one new iridoid glucoside monomer (6), other known iridoid glycosides loganin, cantleyoside, triplostoside A, lisianthioside, 6'-O-beta-D-apiofuranosyl sweroside, as well as triterpenoids oleanic acid and akebiasaponin D. The structures of new compounds 1-6 were determined as dipsanosides C (1), D (2), E (3), F (4), G (5), and 3'-O-beta-D-glucopyranosyl sweroside (6) by spectroscopic, including 1D and 2D NMR techniques, and chemical methods.     

Anti-neuraminidase activity of chemical constituents of Balanophora involucrata

Balanophora involucrata J. D. Hooker has been known to possess potential anti-inflammatory and antibacterial activities; however, its antiviral activity has not been evaluated so far. In order to find new neuraminidase inhibitors (NAIs), the neuraminidase (NA) inhibition activity of different B. involucrata extracts was evaluated. In this study, an in vitro NA inhibition assay was performed to identify which extract of B. involucrata exhibits (maximal) inhibitory activity against NA. Ultra high performance liquid chromatography/quadrupole time-of-flight–tandem mass spectroscopy (MS/MS) and molecular docking techniques were used to identify the specific compounds responsible for the anti-influenza activity of the extract, and to explore the potential natural NAIs. The ethyl acetate extract of B. involucrata exhibited significant inhibitory activity against NA with 50% inhibitory concentration (IC₅₀) value of 159.5 μg/mL. Twenty compounds were identified according to the MS/MS spectra; among them two compounds (quercitrin and phloridzin) showed obvious inhibitory activity against NA, with IC₅₀ of 311.76 and 347.32 μmol/L, respectively. This study suggested that B. involucrata can be a potential natural source of NAIs and may be useful in the fight against ferocious influenza viruses.     

阿尔泰狗娃花化学成分研究

本文从阿尔泰狗娃花Heteropappus altaicus的乙醇提取物中分离出12个已知化合物,经过红外光谱(IR),质谱(MS),核磁共振谱(NMR)等分析方法及与标准品对照,鉴定了它们的结构。分别为:木栓酮(1),表木栓醇(2),达玛烷-20,24-二烯-3β-醇(3),达玛烷-20,24-二烯-3β-乙酰基(4),α-波甾醇(5),槲皮素(6),3'-甲氧基槲皮素(7),槲皮素-3-O-β-D-葡萄糖苷(8),阿魏酸(9),β-谷甾醇(10),β-胡萝卜苷(11),没食子酸乙酯(12)。其中化合物9和12为首次从该属植物中分离得到,化合物5,6,9和12为首次从该植物中分离得到。     

西沙仙掌藻Halimeda xishensis的化学成分研究(Ⅰ)

对西沙仙掌藻Halimeda xishaensis样品进行化学成分研究,从中分离得到了三个化合物,其结构通过MS、NMR及X射线单晶衍射等波谱方法进行鉴定,确定结构为:1-正二十三碳酸甘油酯(Ⅰ),豆甾-4-烯-3β,6β-二醇(Ⅱ),丁四醇(Ⅲ);对此样品的进一步研究正在进行中.     

Bioactive chemical constituents from Hyptis suaveolens stem

Our previous studies have found that the aerial part of Hyptis rhomboides and the seed of Hyptis suaveolens contain xanthine oxidase (XO) inhibitors, inspiring us to investigate the chemical constituents of H. suaveolens stem. The EtOH extract of H. suaveolens stem was fractionated by liquid-liquid partitioning, followed by separation over Sephadex LH-20, silica gel, and reverse-phase column chromatography, centrifugal partition chromatography, and semi-preparative RP-HPLC, to give 17 bioactive isolates. Of these, dimethyl melitrate A ( 9 ) is a new natural product, 10 is likely (7″ E)-9′-methyl melitrate A, only caffeic acid ( 2 ) is the same as those isolated from the seed of the same plant, and only nepetoidins A ( 14 ) and B ( 11 ) are the same as those from H. rhomboides stem. Nepetoidin B ( 11 ) showed the best anti-XO activity. Rosmarinic acid ( 3 ) is the most abundant (>661 ppm; w/w, dried stem), while melitric acid ( 12 ) (>277 ppm), caffeic acid ( 2 ; >125 ppm), and methyl rosmarinate ( 4 ; >81 ppm) are the major ones. This outcome could serve as a good basis for chemotaxonomy of the Hyptis plants and is beneficial for developing H. suaveolens stem as anti-hyperuricemic nutraceutical.     

不同茯苓化学成分的研究

本文测定了天然茯苓、发酵茯苓、药性发酵茯苓和复合药性发酵茯苓的多糖、总糖、氨基酸和微量元素的含量。研究结果表明不同茯苓的化学成分有较大差异,探讨了上述差异产生的原因。上述研究为茯苓相关保健食品和药品的开发提供了科学依据和参考。     

见血飞化学成分的研究 I

从见血飞根茎的乙醇提取物中的脂溶性部分, 分得6个化合物, 它们经鉴定为pimpincollin(1), toddaloactone(2), 5-hydroxy-4,8-dimethoxy-furoquinoline(3),skimmianino(4), isopimpinellin(5)和11-acetonyl-dihydronitidine(6), 其中3,6为新生物碱。     

Studies on the chemical constituents of Phyllanthus emblica

Phytochemical investigations on Phyllanthus emblica have resulted in the isolation of the two new flavonoids, kaempferol-3-O-alpha-L-(6'-methyl)-rhamnopyranoside (1) and kaempferol-3-O-alpha-L-(6'-ethyl)-rhamnopyranoside (2). Their structures were determined on the basis of extensive spectroscopic studies including 2D-NMR experiments.     

云南大花红景天化学成分研究

从云南丽江产民间药物大花红景天(Rhodiola crenulata)根的乙醇提取物中分离得到10个化合物, 经化学方法和光谱数据分析, 10个化合物结构鉴定为: 山奈酚(1), 山奈酚7-O-α-L-鼠李糖苷(2), 大花红景天苷(3), 草质素7-O-α-L-鼠李糖苷(4), 草质素7-O-(3"-O-β-D-葡萄糖基)-α-L-鼠李糖苷(5), 大花红景天素(6),鞣花酸(7), 红景天苷(8), 没食子酸(9), 没食子酸乙酯(10)。其中化合物1, 2,3, 6, 7是首次从大花红景天中得到, 化合物3-为一新的山奈酚苷, 用13^C NMR,1^H-1^H COSY, 13^C-1^H COSY谱确证6为一种结构罕见的黄酮醇与苯丙素结合而成的化合物。     

细穗玄参的化学成分

细穗玄参(Scrofella chinensis Maxim)为玄科细穗玄参属独种植物,它是我国特有种,被用作传统中药.从细穗玄参全草中分离并鉴定了7个化合物.利用光谱(MS、NMR、UV)和化学方法分别鉴定为对苯酚(t),对甲氧基苯甲酸(2),5,7,3＇-三羟基-4-甲氧基黄酮(3),3,7-二羟基5,4＇-二甲氧基黄酮(4),5,6,7-三羟基-4＇-甲氧基黄酮(5),熊果甙(6),玉叶金花糖甙酸(7).7个化合物均为首次从该植物中发现.     

New chemical constituents of roots of Urtica triangularis HAND-MASS

Studies on the chemical constituents of roots of Urtica triangularis HAND-MASS have led to the isolation of four new compounds. The structures, including the absolute configurations, of these constituents have been elucidated through spectral studies including (1)H-NMR, (13)C-NMR, 2D-NMR experiments (heteronuclear single-quantum coherence, heteronuclear multiple bonding connectivity and nuclear Overhauser effect spectroscopy), high resolution mass spectroscopy (HR-MS) and circular dichroism as (-)-4-methoxy-8'-acetyl olivil, (-)-4-methoxy-8'-acetyl olivil-4-O-alpha-arabinopyronosyl-(1-->6)-beta-glucopyranoside, (-)-olivil-9-O-beta-glucopyranoside and cyclo-olivil-9-O-beta-glucopyranoside.     

The chemical constituents of Capparis spinosa of Jordanian origin

Investigation of Capparis spinosa of Jordanian origin lead to isolation of two new compounds beta-sitosterylglucoside-6'-octadecanoate (1) and 3-methyl-2-butenyl-beta-glucoside (2). Linked Scan MS measurements were used to propose a mass fragmentation pattern for the alkaloid Cadabicine isolated here for the second time from nature.