室温离子液体在色谱分离中的应用进展

室温离子液体作为21世纪的新型溶剂,近几年在分析领域应用广泛.本文重点综述了离子液体在气相色谱、液相色谱、毛细管电泳及薄层色谱等方面的应用进展,并对其在色谱分离中的研究进行了展望.     

Two‐dimensional separation techniques using supercritical fluid chromatography

High‐resolution separation systems are essential for the analysis of complex mixtures in a wide variety of application areas. To increase resolution, multidimensional chromatographic techniques have been one key solution. Supercritical fluid chromatography provides a unique opportunity in these multidimensional separations based on its potential for high solvent compatibility, rapid duty cycles, and orthogonality to other separation modes. This review focuses on two‐dimensional chromatography methods from the past decade that use supercritical fluid chromatography because of these advantages. Valving schemes and modulation strategies used to interface supercritical fluid chromatography with other liquid chromatography and gas chromatography techniques are described. Particular applications of multidimensional separations using supercritical fluid chromatography for the analysis of oils and chiral separations of pharmaceutical compounds are highlighted. Limitations of and a potential trajectory for supercritical fluid chromatography in this field are also discussed.     

离子色谱法分析金属离子的研究进展

综述了离子色谱法(IC)分析金属离子的研究进展,对目前应用于分析金属离子的阳离子交换IC、阴离子交换IC和螯合离子色谱进行了评述。阳离子交换IC是IC分析金属离子的主要形式,固定相为强酸(磺酸)型阳离子交换剂和弱酸(羧酸)型阳离子交换剂,结合适当的检测方法,阳离子交换IC可以测定碱金属、碱土金属、过渡金属、稀土离子、铵离子及低相对分子质量的有机胺类分子等。阴离子交换IC可以分析碱土金属、过渡金属、稀土离子等,对金属离子的分析具有更好的选择性,并可以实现金属离子和无机阴离子的同时测定。螯合离子色谱可以对复杂基体中的痕量金属离子进行测定。引用文献125篇。     

砷形态分析方法进展

对光变分析法,高效液相色谱法,毛细管电泳法以及气相色谱法在砷形态分析中的应用进行了评述,比较了各种方法的优缺点,引用文献82篇。     

氨基酸的分析方法及其应用进展

从衍生试剂角度,介绍了不同衍生化氨基酸的分析方法,包括离子交换色谱法、高效液相色谱法、气相谱法和毛细管电泳法,以及无需衍生化的直接分析法高效阴离子交换色谱-积分脉冲安培法,并总结了蛋白质、食品和生理体液样品中的氨基酸分析方法。     

Recent advances of online coupling of sample preparation techniques with ultra high performance liquid chromatography and supercritical fluid chromatography

Ultra high performance liquid chromatography and supercritical fluid chromatography techniques are favored because of their high efficiency and fast analysis speed. Although many sample preparation techniques have been coupled with common liquid chromatography online, the online coupling of sample preparation with the two popular chromatography techniques have gained increasing attention owing to the increasing requirements of efficiency and sensitivity. In this review, we have discussed and summarized the recent advances of the online coupling of sample preparation with ultra high performance liquid chromatography and supercritical fluid chromatography techniques. The main sample preparation techniques that have been coupled with ultra high performance liquid chromatography online are solid‐phase extraction and in‐tube solid‐phase microextraction, while solid‐phase extraction and supercritical fluid extraction are the main techniques that have been coupled with supercritical fluid chromatography online. Especially, the strategies for online coupling of sample preparation with chromatography techniques were summarized. Typical applications and growing trends of the online coupling techniques were also discussed in detail. With the increasing demands of improving the efficiency, throughput, and analytical capability toward complex samples of the analysis methods, online coupling of sample preparation with chromatography techniques will acquire further development.     

色谱专家系统的应用和发展

随着科学技术的发展,色谱专家系统作为一种人工智能方法亦在不断发展,并在实际工作中发挥着越来越重要的作用。该文综述了作者的研究小组在色谱专家系统研究方面的进展,重点介绍从研究气相色谱专家系统开始,到应用色谱专家系统的思想,运用气相色谱、液相色谱和毛细管电泳新技术解决石化、环境、疾病诊断、基因分析和药物分析等领域实际问题的工作。引用相关文献64篇。     

Recent stationary phase-based fractionation strategies in proteomic analysis

In this article, we review stationary phase-assisted sample prefractionation in proteomic analysis in 2019–2022 period. Applications are grouped according to the mode of retention used in the prefractionation step prior to the final analysis by low-pH reversed-phase liquid chromatography-tandem mass spectrometry. Online or offline instrumental configurations are also commented on, with a highlight on novel online platforms. From the articles reviewed in this period, the order of popularity of chromatographic modes for sample fractionation is affinity chromatography > size exclusion chromatography > hydrophilic interaction chromatography > high-pH reversed-phase liquid chromatography > ion exchange chromatography.     

Advances in native high‐performance liquid chromatography and intact mass spectrometry for the characterization of biopharmaceutical products

The characterization of biotherapeutics represents a major analytical challenge. This review discusses the current state‐of‐the‐art in analytical technologies to profile biopharma products under native conditions, i.e., the protein three dimensional conformation is maintained during liquid chromatographic analysis. Native liquid‐chromatographic modes that are discussed include aqueous size‐exclusion chromatography, hydrophobic interaction chromatography, and ion‐exchange chromatography. Infusion conditions and the possibilities and limitations to hyphenate native liquid chromatography to mass spectrometry are discussed. Furthermore, the applicability of native liquid‐chromatography methods and intact mass spectrometry analysis for the characterization of monoclonal antibodies and antibody–drug conjugates is discussed.     

现代分析检测技术在润滑油氧化中的应用

介绍了近20年来用于润滑油氧化测试中分析检测技术的进展情况,包括高效液相色谱、气相色谱、凝胶渗透色谱、热分析、红外光谱、核磁共振、化学发光及电化学技术,并讨论了今后发展的方向。     

离子液体在色谱中的应用

离子液体是当前化学研究领域的一个热点,它在化学的各个领域都有研究和应用。该文就离子液体在毛细管电泳、气相色谱、高效液相色谱中的应用研究进展进行了较为详细的评述,对离子液体的分离检测作了简单的介绍,并对离子液体在色谱研究应用中的发展进行了展望。     

多维高效液相色谱分离模式组合

简述了多维高效液相色谱法的特点及发展简况,重点对分子排阻色谱／反相色谱、离子交换色谱／反相色谱、正相色谱／反相色谱、分子排阻色谱／离子交换色谱、液固色谱／反相色谱、亲合色谱／反相色谱、非手性柱／手性柱等的联用模式及实际应用进行了概括和总结。     

液相色谱法分离金属螯合物的进展

综述了用薄层液相色谱法和柱液相色谱法分离金属螯合物的进展及其在金属离子分析方面的应用。     

Extraction techniques based on solid state and connected with liquid chromatography

Extraction methods applied in analysis of water samples can be named as liquid chromatography. Very often, these techniques are used as sample preparation method before another analytical method such gas chromatography or high performance liquid chromatography. The subject of this review is to compare the extraction techniques of liquid samples and discuss their characteristics in comparison with liquid chromatography. Some new extraction techniques are described, and some characteristic parameters are compared.     

茶多酚的色谱分析法

 对茶多酚的主要成分――儿茶素的色谱分析法进行了综述。重点介绍了应用最多的反相高 效液相色谱法,同时简述了平板色谱法、气相色谱法以及目前出现的新的色谱分析方法。     

氨基酸分析方法的研究进展

对目前应用于氨基酸分析的主要方法作了较详细评述。这些方法包括柱后衍生高效阳离子交换色谱法、柱前衍生反相高效液相色谱法、高效阴离子交换色谱-积分脉冲安培检测法等。引用文献73篇。     

Liquid chromatographic purification and detection of anabolic compounds.

The role of liquid chromatography within methods of analysis for steroids, related compounds and beta-agonists in biological samples is discussed. Special attention is given to the application of liquid chromatography in sample preparation and extract clean-up. Different forms of liquid chromatography, including immunoaffinity chromatography, are compared and evaluated. Methods for confirmation based on gas chromatography-mass spectrometry and cryotrapping Fourier transform infrared spectrometry are discussed.     

Determination of isocyanates in air

Procedures for the determination of isocyanates in air are considered, including procedures for air sampling with the subsequent analysis and on-line monitoring procedures. Only chemisorption is used for the sampling and preconcentration of isocyanates because of their high reactivity. The data on the reagents in use, the types of sampling devices, and operation conditions are surveyed. Presently, high-performance liquid chromatography is primarily used for the subsequent instrumental analysis of samples. Photometric techniques were used in early studies; more recently, thin-layer chromatography and gas chromatography were introduced. Examples of analysis using other techniques are very few. Methods of on-line monitoring of air (both instrumental techniques and rapidly developed biomonitoring, which is performed simultaneously with the analysis of polluted air) are briefly considered.     

Design of experiment techniques for the optimization of chromatographic analysis conditions: A review

Design of experiment (DoE) techniques have been widely used in the field of chromatographic parameters optimization as a valuable tool. A systematic literature review of the available DoE techniques applied to the development of a chromatographic analysis method is presented in this paper. First, the most common available designs and the implementation steps of DoE are comprehensively introduced. Then the studies in recent 10 years for the application of DoE techniques in various chromatographic techniques are discussed, such as capillary electrophoresis, liquid chromatography, gas chromatography, thin-layer chromatography, and high-speed countercurrent chromatography. Current problems and future outlooks are finally given to provide a certain inspiration of research in the application of DoE techniques to the different chromatographic techniques field. This review contributes to a better understanding of the DoE techniques for the efficient optimization of chromatographic analysis conditions, especially for the analysis of complex systems, such as multicomponent drugs and natural products.     

多维液相柱色谱

多维液相柱色谱分离分析复杂样品越来越受到重视。本文介绍了国内外多维液相柱色谱的近期发展,详细讨论了二维液相色谱的实现,其中包括固定相、流动相的选择、温度变量的作用以及两维间切换的实现,并对多维液相柱色谱的应用现状进行了总结。