共查询到20条相似文献,搜索用时 15 毫秒
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Jan Petr 《Chromatographia》2014,77(21-22):1413-1414
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Takao TSUDA 《色谱》2000,18(5):402-411
Abstract:Capillary columns are used in both capillary liquid chromatography and capillary electrochromatography. The design for capillary liquid chromatography is discussed in comparison with capillary gas chromatography. The difference of diffusion coefficient in gas and liquid phase is a key role. The study for obtaining a high performance capillary liquid chromatography is discussed. Capillary electrochromatography is recently interesting for its instinct ability to realize a high performance chromatography. Capillary electrochromatography with and without pressurized flow is reviewed briefly. Instrumentation for capillary electrochromatography with pressurized flow is discussed. The port of splitting, and gradient elution of both solution and potential are described. The new findings of both the variation of column resistance and capacity factor according to the value of applied electric voltage are also discussed. 相似文献
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ZHANG Tian-You 《高等学校化学学报》1999,20(Z1):217
Countercurrent Chromatography (CCC) is a special form liquid-liquid partition chromatography which entirely eliminates the use of solid support matrix. Its development began in the mid-1960s and first introduction of CCC is in 1970. In its early stage of development, the method was hindered by a limited mobile flow rate since the application of higher flow rates resulted in excessive loss of the stationary phase from the column. 相似文献
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The growing interest in proteomics and genomics needs analytical techniques capable of detecting proteins and nucleic acids at the ng L?1 levels due to the small amounts of various proteins and nucleic acids in living cells. During the last decade remarkable development has occurred in the evolution of chromatographic and capillary electrophotetic methods on microchips. These methods are called nano liquid chromatography (NLC) and nano capillary electrophoresis (NCE). The present review article describes the application of NLC and NCE to proteomics and genomics research The analyses of proteins and nucleic acids at low levels is described together with the optimization and future trends of nano methods in genomics and proteomics. 相似文献
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Edward S. Yeung 《中国化学会会志》1991,38(4):307-312
Two new approaches for absorption detection in capillary columns are presented. They are applicable to columns as narrow as 10μm i.d. Both provide good sensitivity despite the very short optical pathlengths that are commonly available in commercial systems. The first involves irradiating the column along its axis rather than across the axis. The entire analyte zone can then participate in absorption. Gains up to 1000 fold can be expected. The second is a modification of laser-excited fluorescence. The absorbing analytes, even though they do not fluoresce efficiently, can transfer their energy to a fluorophore added to the mobile phase. The secondary emission can be used to detect absorption in the nanomolar range. 相似文献
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细胞是生命活动的基本单位,单细胞分析能够阐明细胞的结构与功能,揭示细胞之间的差异性,在细胞分化、生理病理研究及疾病早期诊断中具有重要作用。毛细管电泳作为一种高效的分离手段,所需样品体积小,能与高灵敏度检测器联用,特别适于单细胞分析。本文从单细胞进样技术、单细胞裂解技术、检测方法及应用等方面,对2007年以来毛细管电泳用于单细胞分析的最新进展做了相应叙述,并对未来的发展方向进行了展望。表明毛细管电泳不仅适于单细胞分析,而且仍有巨大的发展潜力和广阔的应用空间。 相似文献